Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 603,393 T→A 5.1% intergenic (+58/+23) pheP → / ← ybdG phenylalanine transporter/mechanosensitive channel protein, miniconductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913603,3930TA 5.1% 675.4 / 18.0 190intergenic (+58/+23)pheP/ybdGphenylalanine transporter/mechanosensitive channel protein, miniconductance
Reads supporting (aligned to +/- strand):  ref base T (85/94);  new base A (2/8);  total (87/103)
Fisher's exact test for biased strand distribution p-value = 1.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.36e-01

Only 100 of 195 total aligned reads displayed.

GTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTCCTCGGCATGATTTTGCTGCTGATGTGCACGATGGATGATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCATATTCCAGCCACACCACGGTGTTAGTAAACGCGTAGATCTCGAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCAT  >  NC_000913/603130‑603631
                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              
                                             aGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTCCTCGGCATGATTTTGCTGCTGATGTGCACGATGGATGATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTa                                                                                                                                                                                                                       <  2:259905/244‑1 (MQ=255)
                                                         aTCCGTTCGGCAACTATCTCTGCATTGCCTTCCTCGGCATGATTTTGCTGCTGATGTGCACGATGGATGATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTa                                                                                                                                                                                                                       <  4:277425/232‑1 (MQ=255)
                                                               tCGGCAACTATCTCTGCATTGCCTTCCTCGGCATGATTTTGCTGCTGATGTGCACGATGGATGATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATAAGAGCGGTCTTTTTAATTACTGCTTAAATGCACCCGCAAGAGAGCGAATATCATTGCTGGTTGGCgtc                                                                                                                                                                      >  2:183680/1‑273 (MQ=255)
                                                                                                   ttttGCTGCTGAGGTGTACGATGGGTGATATGCGCTTGTCATCGATTCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGTGATTTAAAGCGCTGCGTCGGAAATAAGGCAGTCACGCTACATCCGACAAATCGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTGTTTATTACTGCTTAAATGCACCCCGAGGGAGCGAATATCATTGACGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTACGACAGTCGCaa                                                                                                                                 <  4:117176/275‑1 (MQ=255)
                                                                                                            tgatttgagcGATGGATGCTATGCGCTTGTCAGTGATCCTGCTTCCGGTGTTGATTGTATTCCTGTTTATGGCATTTAAAACGATGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATAtgtg                                                                                                                          <  1:4210/265‑1 (MQ=255)
                                                                                                                   cACGATGGATGATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCAt                                                                                                                                                                                                                                                >  8:444023/1‑149 (MQ=255)
                                                                                                                   cACGATGGATGATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCAGTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCAt                                                                                                                                                                                                                                                >  6:738913/1‑149 (MQ=255)
                                                                                                                    aCGATGGATGATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCAtt                                                                                                                                                                                                                                               <  7:169514/149‑1 (MQ=255)
                                                                                                                      gatggatgATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCg                                                                                                                                                                                                                                             >  8:878158/1‑149 (MQ=255)
                                                                                                                      gatggatgATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCg                                                                                                                                                                                                                                             >  8:1338392/1‑149 (MQ=255)
                                                                                                                        tggatgATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGAt                                                                                                                                                                                                                                           <  5:520888/149‑1 (MQ=255)
                                                                                                                          gatgatATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAATGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGc                                                                                                           <  2:149979/275‑1 (MQ=255)
                                                                                                                          gatgatATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATga                                                                                                                                                                                                                                         >  8:461916/1‑149 (MQ=255)
                                                                                                                          gatgatATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATga                                                                                                                                                                                                                                         >  5:854291/1‑149 (MQ=255)
                                                                                                                          gatgatATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATga                                                                                                                                                                                                                                         <  5:74315/149‑1 (MQ=255)
                                                                                                                            tgatATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCGAATGGATgaga                                                                                                                                                                                                                                       <  7:551168/149‑1 (MQ=255)
                                                                                                                             gatATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGagag                                                                                                                                                                                                                                      >  8:732649/1‑149 (MQ=255)
                                                                                                                              atatGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCGAATGGATGAGAGc                                                                                                                                                                                                                                     <  5:1371821/149‑1 (MQ=255)
                                                                                                                               tatGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCGAATGGATGAGAGCg                                                                                                                                                                                                                                    <  5:672217/149‑1 (MQ=255)
                                                                                                                               tatGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCg                                                                                                                                                                                                                                    <  8:1416642/149‑1 (MQ=255)
                                                                                                                                 tGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGt                                                                                                                                                                                                                                  >  7:581001/1‑149 (MQ=255)
                                                                                                                                   cgcTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGttt                                                                                                                                                                                                                                <  5:1299527/149‑1 (MQ=255)
                                                                                                                                    gcTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGtttt                                                                                                                                                                                                                               <  6:1022128/149‑1 (MQ=255)
                                                                                                                                       tGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCCTCCGAAACCGCTCTCATCCATTCGATGAGAGCGGttttttt                                                                                                                                                                                                                            <  7:951845/149‑1 (MQ=255)
                                                                                                                                       tGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGttttttt                                                                                                                                                                                                                            <  8:451433/149‑1 (MQ=255)
                                                                                                                                         tCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTaa                                                                                                                                                                                                                          <  5:1254377/149‑1 (MQ=255)
                                                                                                                                          cAGCTATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAAt                                                                                                                                                                                                                         <  7:732649/149‑1 (MQ=255)
                                                                                                                                          cAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAAt                                                                                                                                                                                                                         <  6:1441578/149‑1 (MQ=255)
                                                                                                                                             cGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTAc                                                                                                                                                                                                                      <  7:1334137/149‑1 (MQ=255)
                                                                                                                                                tCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGc                                                                                                                                                                                                                   >  6:706156/1‑149 (MQ=255)
                                                                                                                                                tCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGc                                                                                                                                                                                                                   <  6:1065536/149‑1 (MQ=255)
                                                                                                                                                  ctgctgCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCtt                                                                                                                                                                                                                 <  6:1280605/149‑1 (MQ=255)
                                                                                                                                                       gcCGGGGTGAATTGTTTTCCAGTTTATGGAATTAAAAACGCTGCATCGAAAATAAGGCATTAACGGTAAATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCAATTCGATGAGAGCGGTTTGTTTAAGTACTGCTTAAAGGCACACGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCATAGTCCAGCCAcacca                                                                              <  4:50034/275‑1 (MQ=255)
                                                                                                                                                           gtgtGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCAcc                                                                                                                                                                                                        >  7:743379/1‑149 (MQ=255)
                                                                                                                                                              tgGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGc                                                                                                                                                                                                     <  7:384524/149‑1 (MQ=255)
                                                                                                                                                                gATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCa                                                                                                                                                                                                   >  5:1115793/1‑149 (MQ=255)
                                                                                                                                                                 aTTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCag                                                                                                                                                                                                  >  6:752647/1‑149 (MQ=255)
                                                                                                                                                                 aTTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCag                                                                                                                                                                                                  >  7:393690/1‑149 (MQ=255)
                                                                                                                                                                      aTTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGa                                                                                                                                                                                                                                            <  5:602543/102‑1 (MQ=255)
                                                                                                                                                                      aTTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGa                                                                                                                                                                                                                                            >  6:602543/1‑102 (MQ=255)
                                                                                                                                                                      aTTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGc                                                                                                                                                                                             <  7:527971/149‑1 (MQ=255)
                                                                                                                                                                       ttCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCg                                                                                                                                                                                            <  5:706156/149‑1 (MQ=255)
                                                                                                                                                                         ccTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAGCCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGaa                                                                                                                                                                                          <  5:457319/149‑1 (MQ=255)
                                                                                                                                                                             tttATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGAt                                                                                                                                                                                                                                           >  8:635197/1‑96 (MQ=255)
                                                                                                                                                                             tttATGGCATTTAAAAAGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCCTTCGAt                                                                                                                                                                                                                                           <  7:635197/96‑1 (MQ=255)
                                                                                                                                                                               tATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCAt                                                                                                                                                                                    <  8:153769/149‑1 (MQ=255)
                                                                                                                                                                               tATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATAAATCAGCCTGAATGCTTTCATATTCCAGCCACACCACGGGTTTAGTAAACGCGTAGAttc                                                      >  4:215743/1‑273 (MQ=255)
                                                                                                                                                                                aTGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCAtt                                                                                                                                                                                   <  8:393690/149‑1 (MQ=255)
                                                                                                                                                                                     aTTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGcc                                                                                                                                                                                                    <  8:314876/127‑1 (MQ=255)
                                                                                                                                                                                      tttAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGt                                                                                                                                                                             >  7:747977/1‑149 (MQ=255)
                                                                                                                                                                                      tttAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGt                                                                                                                                                                             >  8:1037587/1‑149 (MQ=255)
                                                                                                                                                                                       ttAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGtt                                                                                                                                                                            <  8:581001/149‑1 (MQ=255)
                                                                                                                                                                                       ttAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGtt                                                                                                                                                                            <  8:407568/149‑1 (MQ=255)
                                                                                                                                                                                          aaaCGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTaaa                                                                                                                                                                                                              <  5:558275/112‑1 (MQ=255)
                                                                                                                                                                                                tGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCATATTCCAGCCACACCACGGTGTTAGTAAACGCGTAGATCTCGAGCGGTAAACCGTTa                                     <  2:215743/275‑1 (MQ=255)
                                                                                                                                                                                                 gCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGTGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACtgat                                                                                                                                                                  <  6:51423/149‑1 (MQ=255)
                                                                                                                                                                                                  cGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTATCATCCATTCGATGAGAGCGGTTTTTTTAATTTCCGCTTAAATGCACCCGCCAGAGAGCGAATATCAATGCCGGTTGGCGACtgatg                                                                                                                                                                 <  8:1238630/149‑1 (MQ=255)
                                                                                                                                                                                                    tCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGaa                                                                                                                                                               >  7:805319/1‑149 (MQ=255)
                                                                                                                                                                                                    tCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGaa                                                                                                                                                               >  6:488777/1‑149 (MQ=255)
                                                                                                                                                                                                         aaTAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGc                                                                                                                                                          >  7:129783/1‑149 (MQ=255)
                                                                                                                                                                                                          aTAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCa                                                                                                                                                         <  7:305002/149‑1 (MQ=255)
                                                                                                                                                                                                            aaGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGAt                                                                                                                                                                                                                                           >  6:982350/1‑65 (MQ=255)
                                                                                                                                                                                                            aaGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGa                                                                                                                                                       <  6:906400/149‑1 (MQ=255)
                                                                                                                                                                                                            aaGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGa                                                                                                                                                       >  7:350190/1‑149 (MQ=255)
                                                                                                                                                                                                              ggCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGAcc                                                                                                                                                     >  8:1237378/1‑149 (MQ=255)
                                                                                                                                                                                                                cATTCATGCTACATCCGAAAAAACGAAGTCAACCTTCCGAAACCACTCTCATCCTGTGGATGAGGGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCATATTCCAGCCACACCACGGTGTTAGTAAACGCGTAGATCTCGAGCGGTAAACCGTTATCACCTGGGGCCAGt                      <  4:183680/274‑1 (MQ=255)
                                                                                                                                                                                                                 aTTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCCGACCaaa                                                                                                                                                  <  6:806072/149‑1 (MQ=255)
                                                                                                                                                                                                                 aTTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCaaa                                                                                                                                                  <  5:1037836/149‑1 (MQ=255)
                                                                                                                                                                                                                      ccgtACATCCGACAAAAAGATGTCAACCATCCGAAACCGCTCTCTTCCATTCGATGAGGGCGGTTTTTTTAATTCCTGCTGAAATGCACCCGCCATAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCATATTCCAGCCACACCACGGTGTTAGTAAACGCGTAGATCTCGAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGt                <  2:210090/271‑1 (MQ=255)
                                                                                                                                                                                                                       gCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTc                                                                                                                                            <  6:458563/149‑1 (MQ=255)
                                                                                                                                                                                                                             tCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAAt                                                                                                                                      <  6:669429/149‑1 (MQ=255)
                                                                                                                                                                                                                              ccGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATc                                                                                                                                     >  5:396366/1‑149 (MQ=255)
                                                                                                                                                                                                                                 aCAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAatat                                                                                                                                                                                       >  7:1077262/1‑96 (MQ=255)
                                                                                                                                                                                                                                   aaaaCGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTc                                                                                                                                                            <  7:457921/121‑1 (MQ=255)
                                                                                                                                                                                                                                   aaaaCGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCaaa                                                                                                                                <  6:1126826/149‑1 (MQ=255)
                                                                                                                                                                                                                                    aaaCGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAat                                                                                                                               >  8:194460/1‑149 (MQ=255)
                                                                                                                                                                                                                                     aaCGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAata                                                                                                                              >  5:259561/1‑149 (MQ=255)
                                                                                                                                                                                                                                     aaCGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCATATTCCAGCCACACCACGGTGTTAGTAAACGCGTAGATCTCGAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCat  <  2:4967/273‑1 (MQ=255)
                                                                                                                                                                                                                                      aCGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAatat                                                                                                                             >  7:1149118/1‑149 (MQ=255)
                                                                                                                                                                                                                                        gATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATAtgt                                                                                                                           >  5:77382/1‑149 (MQ=255)
                                                                                                                                                                                                                                            tCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATc                                                                                                                       <  8:129783/149‑1 (MQ=255)
                                                                                                                                                                                                                                             cAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCg                                                                                                                      >  6:746920/1‑149 (MQ=255)
                                                                                                                                                                                                                                              aaCCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGa                                                                                                                     >  8:75934/1‑149 (MQ=255)
                                                                                                                                                                                                                                              aaCCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGa                                                                                                                     >  5:33280/1‑149 (MQ=255)
                                                                                                                                                                                                                                                 cATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAata                                                                                                                  >  6:292922/1‑149 (MQ=255)
                                                                                                                                                                                                                                                    ccGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCATATACCAGCcac                                                                                  >  4:377635/1‑178 (MQ=255)
                                                                                                                                                                                                                                                     cGAAACCGCTCTCATCCATTGGATGAGAGCGGATTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTACCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATc                                                                                                              >  8:956458/1‑149 (MQ=255)
                                                                                                                                                                                                                                                          ccGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCt                                                                                                         <  7:237723/149‑1 (MQ=255)
                                                                                                                                                                                                                                                             ctctCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGc                                                                                                   >  2:320634/1‑152 (MQ=255)
                                                                                                                                                                                                                                                              tctcATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAata                                                                                                                  <  8:424594/136‑1 (MQ=255)
                                                                                                                                                                                                                                                              tctcATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAata                                                                                                                  >  7:424594/1‑136 (MQ=255)
                                                                                                                                                                                                                                                              tctcATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGc                                                                                                           >  5:778690/1‑143 (MQ=255)
                                                                                                                                                                                                                                                              tctcATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAAt                                                                                                     >  8:649435/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                 cATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCt                                                                                                  <  8:363385/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                 cATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCt                                                                                                  <  7:674157/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                   tCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCATCCGCCAGAGAGCGAATATAATTGCCGGTTGTCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAAGATGTGATCGAATATATAAGCCTAAATGCttt                                                                                                >  7:903196/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                   tCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCttt                                                                                                <  6:1243037/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                      aTTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCat                                                                                             >  5:510316/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                      aTTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCat                                                                                             >  8:1006747/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                       ttCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCata                                                                                            >  8:1130265/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              
GTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTCCTCGGCATGATTTTGCTGCTGATGTGCACGATGGATGATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCATATTCCAGCCACACCACGGTGTTAGTAAACGCGTAGATCTCGAGCGGTAAACCGTTATCACCTGGAGCCAGTTGGCGTACCATTAAGGTCAT  >  NC_000913/603130‑603631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: