Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,235,581 T→A 6.2% intergenic (+174/‑325) pgi → / → yjbE glucosephosphate isomerase/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,235,5810TA 6.2% 403.1 / 11.1 113intergenic (+174/‑325)pgi/yjbEglucosephosphate isomerase/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand):  ref base T (49/57);  new base A (2/5);  total (51/62)
Fisher's exact test for biased strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 119 total aligned reads displayed.

CTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTT  >  NC_000913/4235327‑4235832
                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                           
cTGAAAGATGATAAAGAAATCAGCAGACACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAGTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCa                                                                                                                                                                                                                                         <  2:94495/275‑1 (MQ=255)
          atAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATACCGAAGTTATGTCGGCATTTTTTTATCAGATAAATacc                                                                                                                                                                                                                               >  4:308734/1‑275 (MQ=255)
                           cACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAAc                                                                                                                                                                                                              <  2:35706/275‑1 (MQ=255)
                                                   agtAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGCTATGTAGGCCGGATAAGGCGTTCACGCCGCAACCGGCAACCGATGCCTGATGCGACTCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAAt                                                                                                                                                                                       <  2:308734/272‑1 (MQ=255)
                                                                        cgcgGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGAttt                                                                                                                                                                         >  4:311013/1‑267 (MQ=255)
                                                                            gTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTaa                                                                                                                                                                     <  2:311013/267‑1 (MQ=255)
                                                                                                                 cACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCgg                                                                                                                                                                                                                                                      >  6:1448603/1‑149 (MQ=255)
                                                                                                                    gccgcATCCGGGAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCAt                                                                                                                                                                                                                                                   >  7:1146283/1‑149 (MQ=255)
                                                                                                                    gccgcATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCAt                                                                                                                                                                                                                                                   >  6:168854/1‑149 (MQ=255)
                                                                                                                    gccgcATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACATAACGTCGGCAt                                                                                                                                                                                                                                                   <  7:1090469/149‑1 (MQ=255)
                                                                                                                     ccgcATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACATAACGTCGGCAtt                                                                                                                                                                                                                                                  <  7:1109006/149‑1 (MQ=255)
                                                                                                                       gcATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCAtttt                                                                                                                                                                                                                                                >  5:839795/1‑149 (MQ=255)
                                                                                                                         aTCAGGCATCGGTTGCCGGATGCGGCGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGCTTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCAtttttt                                                                                                                                                                                                                                              >  5:388279/1‑149 (MQ=255)
                                                                                                                         aTCAGGCATCGGTTGCCGGATGCGACGCGGTCGCGTCTTATCAGGCCTACACGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCAtttttt                                                                                                                                                                                                                                              >  5:1012818/1‑149 (MQ=255)
                                                                                                                           ccGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTa                                                                                                                                                                                                                                            <  5:458557/149‑1 (MQ=255)
                                                                                                                           ccGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTa                                                                                                                                                                                                                                            <  8:1416886/149‑1 (MQ=255)
                                                                                                                            cGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTAt                                                                                                                                                                                                                                           >  7:841818/1‑149 (MQ=255)
                                                                                                                              gCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACGTAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCa                                                                                                                                                                                                                                         <  5:1152141/149‑1 (MQ=255)
                                                                                                                                          tGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCt                                                                                                                                                                                                                             >  7:309965/1‑149 (MQ=255)
                                                                                                                                           gataagACGCGACCGCGTCGCATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCa                                                                                                                                                                                                                                         <  5:1112577/131‑1 (MQ=255)
                                                                                                                                             taagACGCGACCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGt                                                                                                                                                                                                                          >  8:794175/4‑149 (MQ=255)
                                                                                                                                             tGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGt                                                                                                                                                                                                                          <  7:699970/149‑1 (MQ=255)
                                                                                                                                                gACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTa                                                                                                                                                                                         >  2:330506/1‑179 (MQ=255)
                                                                                                                                                 aCGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGt                                                                                                                                                                                                                      <  7:1369559/149‑1 (MQ=255)
                                                                                                                                                   gcgGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTaa                                                                                                                                                                                                                    <  6:99204/149‑1 (MQ=255)
                                                                                                                                                   gcgGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGCCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTaa                                                                                                                                                                                                                    <  5:259235/149‑1 (MQ=255)
                                                                                                                                                    cgGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATcc                                                                                                                                                                                     <  4:330506/179‑1 (MQ=255)
                                                                                                                                                         gcgTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAAc                                                                                                                                                                                                              <  8:825037/149‑1 (MQ=255)
                                                                                                                                                          cgTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACg                                                                                                                                                                                                             <  5:346501/149‑1 (MQ=255)
                                                                                                                                                           gTCTTATCAGGCCTACAGGTCGATGCCGATATGTACCTCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTAATGTCGGCATTTTTTTATCAGATAAATCCCCGTGTCTGTAATTTAACgg                                                                                                                                                                                                            >  8:1230740/1‑149 (MQ=255)
                                                                                                                                                             cTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGt                                                                                                                                                                                                                          >  7:1365622/1‑133 (MQ=255)
                                                                                                                                                             cTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGt                                                                                                                                                                                                                          <  8:1365622/133‑1 (MQ=255)
                                                                                                                                                                 tCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTc                                                                                                                                                                                                                         <  7:1456021/130‑1 (MQ=255)
                                                                                                                                                                 tCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTc                                                                                                                                                                                                                         >  8:1456021/1‑130 (MQ=255)
                                                                                                                                                                   aGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGa                                                                                                                                                                                                                                       >  7:1453463/1‑114 (MQ=255)
                                                                                                                                                                   aGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGa                                                                                                                                                                                                                                       <  8:1453463/114‑1 (MQ=255)
                                                                                                                                                                   aGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATa                                                                                                                                                                                                    <  5:172509/149‑1 (MQ=255)
                                                                                                                                                                   aGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATa                                                                                                                                                                                                    <  5:168854/149‑1 (MQ=255)
                                                                                                                                                                   aGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATa                                                                                                                                                                                                    >  5:868646/1‑149 (MQ=255)
                                                                                                                                                                   aGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATa                                                                                                                                                                                                    <  6:1297502/149‑1 (MQ=255)
                                                                                                                                                                    ggCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATAc                                                                                                                                                                                                   <  5:403133/149‑1 (MQ=255)
                                                                                                                                                                        tACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATAc                                                                                                                                                                                                   >  5:226744/1‑145 (MQ=255)
                                                                                                                                                                        tACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATAc                                                                                                                                                                                                   <  6:226744/145‑1 (MQ=255)
                                                                                                                                                                        tACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTg                                                                                                                                                                                               >  7:1397359/1‑149 (MQ=255)
                                                                                                                                                                          cAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGAt                                                                                                                                                                           >  2:107078/1‑167 (MQ=255)
                                                                                                                                                                              tCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGAttttt                                                                                                                                                                       <  4:107078/167‑1 (MQ=255)
                                                                                                                                                                                gATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAAt                                                                                                                                                                                       >  7:1387681/1‑149 (MQ=255)
                                                                                                                                                                                 aTGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATc                                                                                                                                                                                      <  5:1330987/149‑1 (MQ=255)
                                                                                                                                                                                       atatGTACATCGAATTCAACAATTAATACCTAACCCGATTGATTAAATAACCTTAATAACAATGCTGACGTTATGTCGGCATTTTTTTATCAGATAAGGCCCCTTGGCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTaaaa                                                                                                                                                                                <  6:1012818/149‑1 (MQ=255)
                                                                                                                                                                                         atGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATa                                                                                                                                                                              <  6:199337/149‑1 (MQ=255)
                                                                                                                                                                                           gTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGa                                                                                                                                                                            <  8:81408/149‑1 (MQ=255)
                                                                                                                                                                                           gTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGa                                                                                                                                                                            <  5:1448603/149‑1 (MQ=255)
                                                                                                                                                                                            tACATCGTATTCGGCAATTAATATATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGAt                                                                                                                                                                           >  8:728092/1‑149 (MQ=255)
                                                                                                                                                                                            tACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAGCCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGAt                                                                                                                                                                           <  5:1205800/149‑1 (MQ=255)
                                                                                                                                                                                             aCATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGAtt                                                                                                                                                                          >  8:1287269/1‑149 (MQ=255)
                                                                                                                                                                                               aTCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGAtttt                                                                                                                                                                        <  6:839795/149‑1 (MQ=255)
                                                                                                                                                                                               aTCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGAtttt                                                                                                                                                                        >  5:607701/1‑149 (MQ=255)
                                                                                                                                                                                                tCGTATTCGGCAACTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGAttttt                                                                                                                                                                       <  8:117026/149‑1 (MQ=255)
                                                                                                                                                                                                        ggCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCgttg                                                                                                                                                               >  6:578015/1‑149 (MQ=255)
                                                                                                                                                                                                         gCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCgttgt                                                                                                                                                              <  7:1235505/149‑1 (MQ=255)
                                                                                                                                                                                                           aaTTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGttt                                                                                                                                                            <  7:622753/149‑1 (MQ=255)
                                                                                                                                                                                                             ttAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTAt                                                                                                                                                          >  8:1069159/1‑149 (MQ=255)
                                                                                                                                                                                                                 tACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCg                                                                                                                                                      >  6:393116/1‑149 (MQ=255)
                                                                                                                                                                                                                 tACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCg                                                                                                                                                      >  5:925972/1‑149 (MQ=255)
                                                                                                                                                                                                                    aTAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTc                                                                                                                                                       >  6:1387676/1‑145 (MQ=255)
                                                                                                                                                                                                                      aGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCtt                                                                                                                                                                                                                            >  5:641560/1‑74 (MQ=255)
                                                                                                                                                                                                                         acagttgattAAATAAACTTAATAAAAAAGCCGACGGTATGTTCGGATTTTTTTTACAGATAAATCCCCTTTGCTTTTATTTTACGGAAATTATTCCCTTAGGGTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATAc                                                                                                                                              <  5:618451/145‑1 (MQ=255)
                                                                                                                                                                                                                         aCGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTaaaa                                                                                                                                                                                <  8:270099/115‑1 (MQ=255)
                                                                                                                                                                                                                         aCGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTaaaa                                                                                                                                                                                >  7:270099/1‑115 (MQ=255)
                                                                                                                                                                                                                                 ttAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATc                                                                                                                                                                                                       <  2:73048/84‑1 (MQ=255)
                                                                                                                                                                                                                                 ttAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATAc                                                                                                                                                                                                   <  8:1446257/88‑1 (MQ=255)
                                                                                                                                                                                                                                 ttAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATAc                                                                                                                                              >  8:432543/1‑141 (MQ=255)
                                                                                                                                                                                                                                 ttAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATAc                                                                                                                                              <  7:432543/141‑1 (MQ=255)
                                                                                                                                                                                                                                    aaTAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATc                                                                                                                                                                                                                                          >  6:653916/1‑46 (MQ=255)
                                                                                                                                                                                                                                    aaTAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATc                                                                                                                                                                                                                                          <  5:653916/46‑1 (MQ=255)
                                                                                                                                                                                                                                    aaTAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAAtt                                                                                                                                   <  8:488205/149‑1 (MQ=255)
                                                                                                                                                                                                                                     aTAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTg                                                                                                                                  >  6:264077/1‑149 (MQ=255)
                                                                                                                                                                                                                                        aCCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATcccc                                                                                                                                                                                                                              <  8:864525/54‑1 (MQ=255)
                                                                                                                                                                                                                                          cTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGt                                                                                                                                                                                                                          >  7:1340347/1‑56 (MQ=255)
                                                                                                                                                                                                                                          cTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGt                                                                                                                                                                                                                          <  8:1340347/56‑1 (MQ=255)
                                                                                                                                                                                                                                          cTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGAttttt                                                                                                                                                                       <  5:707738/107‑1 (MQ=255)
                                                                                                                                                                                                                                          cTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGAttttt                                                                                                                                                                       >  6:707738/1‑107 (MQ=255)
                                                                                                                                                                                                                                          cTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCtttt                                                                                                                             >  6:173159/1‑149 (MQ=255)
                                                                                                                                                                                                                                           ttAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTg                                                                                                                            >  7:1198034/1‑149 (MQ=255)
                                                                                                                                                                                                                                            taataaCAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGt                                                                                                                           >  8:294106/1‑149 (MQ=255)
                                                                                                                                                                                                                                              atcaCAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGTAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGttt                                                                                                                         <  6:388279/146‑1 (MQ=255)
                                                                                                                                                                                                                                              ataaCAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGt                                                                                                                                                                                                <  2:146551/78‑1 (MQ=255)
                                                                                                                                                                                                                                              ataaCAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGttt                                                                                                                         <  5:1308583/149‑1 (MQ=255)
                                                                                                                                                                                                                                                  cAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTttttat                                                                                                                     <  7:794175/149‑1 (MQ=255)
                                                                                                                                                                                                                                                   aaTGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTttttatt                                                                                                                    >  7:1183327/1‑149 (MQ=255)
                                                                                                                                                                                                                                                       ccGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGaaaa                                                                                                                                                 >  4:365777/1‑116 (MQ=255)
                                                                                                                                                                                                                                                       ccGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTaa                                                                                                                <  5:1464954/149‑1 (MQ=255)
                                                                                                                                                                                                                                                          aCGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCAt      >  4:282686/1‑252 (MQ=255)
                                                                                                                                                                                                                                                           cGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACg                                                                                                                                             <  2:365778/116‑1 (MQ=255)
                                                                                                                                                                                                                                                             ttATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTAt                                                                                                          <  6:607701/149‑1 (MQ=255)
                                                                                                                                                                                                                                                             ttATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTAt                                                                                                          >  6:861492/1‑149 (MQ=255)
                                                                                                                                                                                                                                                             ttATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTAt                                                                                                          >  7:571614/1‑149 (MQ=255)
                                                                                                                                                                                                                                                             ttATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTAt                                                                                                          <  8:309965/149‑1 (MQ=255)
                                                                                                                                                                                                                                                              tATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATg                                                                                                         <  8:1146283/149‑1 (MQ=255)
                                                                                                                                                                                                                                                              tATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCtt  <  2:282686/252‑1 (MQ=255)
                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                           
CTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTT  >  NC_000913/4235327‑4235832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: