Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 644,030 A→G 5.1% intergenic (‑63/+167) rnk ← / ← rna regulator of nucleoside diphosphate kinase/ribonuclease I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913644,0300AG 5.1% 862.6 / 20.1 269intergenic (‑63/+167)rnk/rnaregulator of nucleoside diphosphate kinase/ribonuclease I
Reads supporting (aligned to +/- strand):  ref base A (118/132);  new base G (9/6);  total (130/139)
Fisher's exact test for biased strand distribution p-value = 4.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-02

Only 100 of 272 total aligned reads displayed.

TTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGAAGACGAATTAGCGTGTTTTGTAAATCAGATGATTAATAACCCGCTTTATCAATCACAAAGGTTTTGCCACAGTTACCTGGGTGAGGTTGTGGCAAGAATGAGTTTGCAGAAAGCGGAGCGTTGATGGCGTCAGCTTTGAT  >  NC_000913/643761‑644304
                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                  
  cGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAg                                                                                                                                                                                                                                                                             >  2:224434/1‑275 (MQ=255)
                   cGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTAGACA‑TTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCAAAAAGAAAACCCTCACCGGCAGGCGGCGAGGGTTTAACTc                                                                                                                                                                                                                                                           >  2:152076/1‑275 (MQ=255)
                          tAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACatga                                                                                                                                                                                                                                                     <  2:214676/275‑1 (MQ=255)
                                  ttGTTCTTTGTCACCACGTGTTGTGGCGACTCATGTGGTGCATCAATTTGGGGGCGTGCCAGCTCTGCGTTTAACCNTTGGGTGGTTGGCAAACCATCATAGGCGGGTTGCTCCAGCAGAATGTCGCTGCGTTCGGCATCAAGGTCGTTAATGATGATAGTTGGTCTGGACATTGTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATact                                                                                                                                                                                                                                              <  3:70362/273‑1 (MQ=255)
                                                                          aaCACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATACCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATGTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACTGTCAGGCGGCGAGGGTTTAACTCACACGATGATACTGACTGTTGCTCACTCGTTGAAGTGATTTGCGTCCCATTcc                                                                                                                                                                                                      >  2:301606/1‑273 (MQ=255)
                                                                              cgtttGGGGGCGATCCAAATCTGCCTTTAACGCGTGGGGGATTGGAAAAGCAGCATAGGCGGGTTGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTGATCCATGTCGTAGGTACTGAGCGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCGCCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCGCTCTTTGAAGTGATTTGCGTCACATTCAGGGaa                                                                                                                                                                                                 <  4:229895/273‑1 (MQ=255)
                                                                                                  ttGCGTTGAACGGGTCGGGGATTGGCAAACCAGCATGGGGGGGTTGCGCCAGCGGAATTTCGAGGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCAtt                                                                                                                                                                              <  4:1066/273‑1 (MQ=255)
                                                                                                     cGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGttt                                                                                                                                                                                                                                                                >  4:172865/1‑189 (MQ=255)
                                                                                                       tttAAGGCGGCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCCGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGtat                                                                                                                                                                        <  2:179249/275‑1 (MQ=255)
                                                                                                                     gATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGcc                                                                                                                                                          <  2:53719/275‑1 (MQ=255)
                                                                                                                      aTTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTaa                                                                                                                                                                                                                                                              >  2:71682/1‑174 (MQ=255)
                                                                                                                            aaaCCAGCATAGGCGGGTTGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCg                                                                                                                                                                                                                                                                                 <  6:1206230/149‑1 (MQ=255)
                                                                                                                             aaCCAGCATATGCGGGTTGTGCCAGCAGAATCACGATGCGTTCGGCATCCAGGGCGTTAATGAGGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTa                                                                                                                                                  <  4:304733/275‑1 (MQ=255)
                                                                                                                             aaCCAGCATAGGCGGGTTGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGt                                                                                                                                                                                                                                                                                <  7:735208/149‑1 (MQ=255)
                                                                                                                               ccAGCATAGGCGGGTTGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCa                                                                                                                                                                                                                                                                              <  5:96729/149‑1 (MQ=255)
                                                                                                                                cAGCATAGGCGGGTTGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAg                                                                                                                                                                                                                                                                             >  6:73660/1‑149 (MQ=255)
                                                                                                                                  gCATAGGCGGGTTGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCa                                                                                                                                                                                                                                                                              >  7:85905/1‑146 (MQ=255)
                                                                                                                                    aTAGGGGGGGTGCTACAGCAGAATAGCGATGCGTTCGGCATCCAGGTCGGTAATGATGAGAGGTGGTCTGGACATTTTTACTCCACGTCGTCGGTGCTGCGCGTGTGGAAGATAAACATACCCAAAAGAAGACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGTTACTGACTGTTGCTCACGCTTTGAAGTGATTTGCGTAACATTCAGGGAATTCCTCAAAGCAGCGCATTATGTATAAATATTAATCGCCTTGGTTTATGGAAg                                                                                                                                            <  4:74961/274‑1 (MQ=255)
                                                                                                                                     tAGGCGGGTTGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAggcggc                                                                                                                                                                                                                                                                        >  6:383346/1‑149 (MQ=255)
                                                                                                                                       ggCGGGTTGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGa                                                                                                                                                                                                                                                                      <  6:247116/149‑1 (MQ=255)
                                                                                                                                            gTTGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGtt                                                                                                                                                                                                                                                                 <  6:612577/149‑1 (MQ=255)
                                                                                                                                             ttGCTCCATCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGttt                                                                                                                                                                                                                                                                >  7:1025950/1‑149 (MQ=255)
                                                                                                                                              tGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCGCCGCCTGACGGTGAGGGtttt                                                                                                                                                                                                                                                               <  5:28719/149‑2 (MQ=255)
                                                                                                                                               gCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTaa                                                                                                                                                                                                                                                              <  8:1399105/149‑1 (MQ=255)
                                                                                                                                                 tCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACt                                                                                                                                                                                                                                                            >  6:1097825/1‑149 (MQ=255)
                                                                                                                                                 tCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACt                                                                                                                                                                                                                                                            >  8:465016/1‑149 (MQ=255)
                                                                                                                                                 tCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACt                                                                                                                                                                                                                                                            >  8:148131/1‑149 (MQ=255)
                                                                                                                                                 tCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGAGGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACt                                                                                                                                                                                                                                                            >  6:193671/1‑149 (MQ=255)
                                                                                                                                                    agcagAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATACACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTcac                                                                                                                                                                                                                                                         >  6:1305259/1‑149 (MQ=255)
                                                                                                                                                      cagAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACt                                                                                                                                                                                                                                                            <  4:356127/144‑1 (MQ=255)
                                                                                                                                                          atatCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACatgatg                                                                                                                                                                                                                                                   <  5:380927/149‑1 (MQ=255)
                                                                                                                                                           tatCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACAtgatga                                                                                                                                                                                                                                                  <  5:1097825/149‑1 (MQ=255)
                                                                                                                                                           tatCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACAtgatga                                                                                                                                                                                                                                                  <  7:48157/149‑1 (MQ=255)
                                                                                                                                                            atCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACatgatg                                                                                                                                                                                                                                                   <  8:1034697/147‑1 (MQ=255)
                                                                                                                                                            atCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATgatgat                                                                                                                                                                                                                                                 >  6:1052840/1‑149 (MQ=255)
                                                                                                                                                               gATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATact                                                                                                                                                                                                                                              >  7:192142/1‑149 (MQ=255)
                                                                                                                                                                aTGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATactg                                                                                                                                                                                                                                             >  7:598893/1‑149 (MQ=255)
                                                                                                                                                                  gCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAACAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATactgac                                                                                                                                                                                                                                           <  5:193671/149‑1 (MQ=255)
                                                                                                                                                                    gTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATactgactg                                                                                                                                                                                                                                         >  5:985390/1‑149 (MQ=255)
                                                                                                                                                                     ttCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGAAGACGAATTAGCGTGTTTTGTAAATCAGATGATtaa                                                                                                          >  2:32544/1‑275 (MQ=255)
                                                                                                                                                                         gCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCT‑‑CTCTTTGAAGTGATTTGCGt                                                                                                                                                                                                              <  1:56242/169‑1 (MQ=255)
                                                                                                                                                                           aTCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATact                                                                                                                                                                                                                                              <  7:67062/137‑1 (MQ=255)
                                                                                                                                                                           aTCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCa                                                                                                                                                                                                                                  <  7:635246/149‑1 (MQ=255)
                                                                                                                                                                           aTCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCa                                                                                                                                                                                                                                  <  5:1265178/149‑1 (MQ=255)
                                                                                                                                                                           aTCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCa                                                                                                                                                                                                                                  >  5:560488/1‑149 (MQ=255)
                                                                                                                                                                               aGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCActct                                                                                                                                                                                                                              >  5:826626/1‑149 (MQ=255)
                                                                                                                                                                                ggccGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCACGTCGTCGGTGCTGCTAGTGTCGCAGATAAACATACCCAAAAGAAAACCATCACCGACGCGCGGCGAGGGTTTTACTCACATGATGATACTGACTGTTGCTCACTCtt                                                                                                                                                                                                                             >  6:826101/4‑149 (MQ=255)
                                                                                                                                                                                    gTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGaa                                                                                                                                                                                                                         <  8:702555/149‑1 (MQ=255)
                                                                                                                                                                                     ttAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGt                                                                                                                                                                                                                                        <  5:624851/133‑1 (MQ=255)
                                                                                                                                                                                      tAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGAAGACGAATTAGCGTGTTTTGTAAATCAGATGATTAATAACCCGCTTTatcaa                                                                                          >  2:25215/1‑274 (MQ=255)
                                                                                                                                                                                        atgatgATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGa                                                                                                                                                                                                                                                                      <  5:1136312/100‑1 (MQ=255)
                                                                                                                                                                                        atgatgATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATact                                                                                                                                                                                                                                              >  5:894899/1‑124 (MQ=255)
                                                                                                                                                                                         tgatgaTAGTTGGTCTGGACATTTTTACTCCATCTCGTCGGTGCTGCGAGTGTCGCAGAAAAACATACCAAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGAt                                                                                                                                                                                                                    >  8:216204/1‑149 (MQ=255)
                                                                                                                                                                                               tAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGt                                                                                                                                                                                                              <  7:1106811/149‑1 (MQ=255)
                                                                                                                                                                                                     gTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACAtt                                                                                                                                                                                                        >  6:67660/1‑149 (MQ=255)
                                                                                                                                                                                                     gTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTCATTTGCGTCACAtt                                                                                                                                                                                                        <  7:318350/149‑1 (MQ=255)
                                                                                                                                                                                                      tCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTc                                                                                                                                                                                                       >  8:1348273/1‑149 (MQ=255)
                                                                                                                                                                                                       cTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCa                                                                                                                                                                                                      <  6:301864/149‑1 (MQ=255)
                                                                                                                                                                                                        tGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAg                                                                                                                                                                                                     >  6:590324/1‑149 (MQ=255)
                                                                                                                                                                                                         ggACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAgg                                                                                                                                                                                                    <  5:1216933/149‑1 (MQ=255)
                                                                                                                                                                                                         ggACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAgg                                                                                                                                                                                                    <  7:42402/149‑1 (MQ=255)
                                                                                                                                                                                                          gACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACCTACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAGCTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGAttt                                                                                                                                                                                                                  <  8:1213280/134‑1 (MQ=255)
                                                                                                                                                                                                          gACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGAttt                                                                                                                                                                                                                  >  7:1213280/1‑134 (MQ=255)
                                                                                                                                                                                                          gACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAggg                                                                                                                                                                                                   >  6:93322/1‑149 (MQ=255)
                                                                                                                                                                                                          gACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAggg                                                                                                                                                                                                   >  8:377259/1‑149 (MQ=255)
                                                                                                                                                                                                          gACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGAAGACGAATTAGCGTGTTTTGTAAATCAGATGATtaataa                                                                                                       >  4:66655/1‑241 (MQ=255)
                                                                                                                                                                                                             aTTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCActct                                                                                                                                                                                                                              <  5:723207/119‑1 (MQ=255)
                                                                                                                                                                                                                    cTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCaa                                                                                                                                                                                         <  6:1394469/149‑1 (MQ=255)
                                                                                                                                                                                                                     tCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGtt                                                                                                                                                                                                                                                                 <  8:652937/76‑1 (MQ=255)
                                                                                                                                                                                                                     tCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAAt                                                                                                                                                                                        >  7:1416198/1‑149 (MQ=255)
                                                                                                                                                                                                                     tCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAAt                                                                                                                                                                                        >  6:1262890/1‑149 (MQ=255)
                                                                                                                                                                                                                        aTGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCa                                                                                                                                                                                     >  5:872937/1‑149 (MQ=255)
                                                                                                                                                                                                                         tGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCAc                                                                                                                                                                                                                                 <  5:1450765/104‑1 (MQ=255)
                                                                                                                                                                                                                         tGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAg                                                                                                                                                                                    >  7:932490/1‑149 (MQ=255)
                                                                                                                                                                                                                             gtcgGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGAAGACGAATTAGCGTGTTTTGTAAATCAGATGATTAATAACCCGCTTTATCAATCACAAAGGTTTTg                                                                            <  4:259529/249‑1 (MQ=255)
                                                                                                                                                                                                                                 gTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTAt                                                                                                                                                                            <  6:542158/149‑1 (MQ=255)
                                                                                                                                                                                                                                     tgcGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGTAATTCCTCAATGCAGCGCATTATGtat                                                                                                                                                                        <  7:1162783/149‑1 (MQ=255)
                                                                                                                                                                                                                                        gAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGAttt                                                                                                                                                                                                                  <  7:135717/104‑1 (MQ=255)
                                                                                                                                                                                                                                           tgtCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCt                                                                                                                                                                  <  8:271349/149‑1 (MQ=255)
                                                                                                                                                                                                                                            gtCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCa                                                                                                                                                                                                                                  >  3:25638/1‑84 (MQ=255)
                                                                                                                                                                                                                                                cAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATc                                                                                                                                                             <  8:356738/149‑1 (MQ=255)
                                                                                                                                                                                                                                                  gATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGc                                                                                                                                                           >  8:1082310/1‑149 (MQ=255)
                                                                                                                                                                                                                                                  gATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGc                                                                                                                                                           >  8:616372/1‑149 (MQ=255)
                                                                                                                                                                                                                                                       aCATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTgg                                                                                                                                                      >  5:1019203/1‑149 (MQ=255)
                                                                                                                                                                                                                                                          tACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGttt                                                                                                                                                   >  7:1327720/1‑149 (MQ=255)
                                                                                                                                                                                                                                                             ccAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATg                                                                                                                                                >  5:1062731/1‑149 (MQ=255)
                                                                                                                                                                                                                                                              cAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATgg                                                                                                                                               >  6:77898/1‑149 (MQ=255)
                                                                                                                                                                                                                                                              cAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATgg                                                                                                                                               >  5:1392926/1‑149 (MQ=255)
                                                                                                                                                                                                                                                              cAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACAGTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATgg                                                                                                                                               >  7:1060147/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                  gttaaaCCCTCGCCGCCTGACGGTGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGAAGa                                                                                                                                           >  7:282197/4‑149 (MQ=255)
                                                                                                                                                                                                                                                                  aGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCtt                                                                                                                                                        >  7:527277/1‑136 (MQ=255)
                                                                                                                                                                                                                                                                  aGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCtt                                                                                                                                                        <  8:527277/136‑1 (MQ=255)
                                                                                                                                                                                                                                                                   ttaaaCCCTCGCCGCCTGACGGTGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGAAGAc                                                                                                                                          >  6:113881/3‑149 (MQ=255)
                                                                                                                                                                                                                                                                   ttaaaCCCTCGCCGCCTGACGGTGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGAAGAc                                                                                                                                          >  7:377009/3‑149 (MQ=255)
                                                                                                                                                                                                                                                                      aaCCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTAt                                                                                                                                                 >  5:527209/1‑139 (MQ=255)
                                                                                                                                                                                                                                                                        cccTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGAAGACGAAtt                                                                                                                                     >  8:1335344/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                           tCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATg                                                                                                                                                <  8:297070/135‑1 (MQ=255)
                                                                                                                                                                                                                                                                           tCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGAAGACGAATTAGc                                                                                                                                  >  8:84819/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                             aCCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGAAGACGAATTAGCgt                                                                                                                                <  7:622919/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                             aCCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGAAGACGAATTAGCGTGTTTTGTAAATCAGATGATTAATAACCCGCTTTATCAATCACAAAGGTTTTGCCACAGTTACCTGGGTGAGGTTGTGGCAAGAATGAGTTTGCAGAAAGCGGCGCGTTGATGGCGTCAGCTTTGAt  >  1:15351/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                  
TTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGAAGACGAATTAGCGTGTTTTGTAAATCAGATGATTAATAACCCGCTTTATCAATCACAAAGGTTTTGCCACAGTTACCTGGGTGAGGTTGTGGCAAGAATGAGTTTGCAGAAAGCGGAGCGTTGATGGCGTCAGCTTTGAT  >  NC_000913/643761‑644304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑