Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,479,020 A→T 7.0% intergenic (+131/+14) rraB → / ← yjgM protein inhibitor of RNase E/GNAT family putative N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,479,0200AT 7.0% 677.0 / 26.6 196intergenic (+131/+14)rraB/yjgMprotein inhibitor of RNase E/GNAT family putative N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (98/84);  new base T (7/7);  total (105/91)
Fisher's exact test for biased strand distribution p-value = 7.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 200 total aligned reads displayed.

AACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGTTTCCAGATAGCAGCGTTTGAAACCCATCTCTCGCGCCTGCTCCATCGCCATTAAGGCCAGTTTTTTTGCCAGCCCTTTGCCGCGGATAGCCGGAAGAAAATACATCTTTTGCAGTTCGCAAATATCCGACTCAC  >  NC_000913/4478756‑4479290
                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                              
aacaacTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGTGAAGATGGCGACGATGAAGATTTTGTCGATGACGACGATGACGGAGTTCGCAACTACTTAANCGTAGCGCATGAGGACCAGGTAGTTCCGGCAACCTGGTGAATTTGTAGCACGGAAAAGGAATTCATTTAACATCNGGAATAAAAAAACAGCAAGTGGTATCGGTTNNAATGCCCGAGAGTTTAACACACAGGCAtg                                                                                                                                                                                                                                                                      >  3:48298/1‑274 (MQ=255)
                                                                            cGAAGATGGCGACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACCTGGCCCGTGCAGCCATGCGCAGACTCGATTCGCTCa                                                                                                                                                                                          >  2:307420/1‑275 (MQ=255)
                                                                            cGAAGATGGCGACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCa                                                                                                                                                                                          >  4:196755/1‑275 (MQ=255)
                                                                                   ggCGACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCa                                                                                                                                                                                   <  2:196755/275‑1 (MQ=255)
                                                                                                                    gACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAaca                                                                                                                                                                                                                                                                                >  7:101060/1‑149 (MQ=255)
                                                                                                                       ggAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACAcgc                                                                                                                                                                                                                                                                             >  8:1029625/1‑149 (MQ=255)
                                                                                                                       ggAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACAcgc                                                                                                                                                                                                                                                                             >  8:696275/1‑149 (MQ=255)
                                                                                                                        gAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGGTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAg                                                                                                                                               <  2:161433/274‑1 (MQ=255)
                                                                                                                         aGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCgg                                                                                                                                                                                                                                                                           <  5:565332/149‑1 (MQ=255)
                                                                                                                         aGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTATAGCCCGGATAAGGAATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGTGTTCAACTCGCgg                                                                                                                                                                                                                                                                           <  5:18966/149‑1 (MQ=255)
                                                                                                                           ttCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGt                                                                                                                                           <  2:55715/275‑1 (MQ=255)
                                                                                                                               ccACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAaca                                                                                                                                                                                                                                                                                >  8:1397854/1‑138 (MQ=255)
                                                                                                                               ccACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAaca                                                                                                                                                                                                                                                                                <  7:1397854/138‑1 (MQ=255)
                                                                                                                                 aCTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCAttttt                                                                                                                                                                                                                                                                   >  8:1060660/1‑149 (MQ=255)
                                                                                                                                  cTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGTGTTCAACTCGCgg                                                                                                                                                                                                                                                                           <  8:760027/140‑1 (MQ=255)
                                                                                                                                  cTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGTGTTCAACTCGCgg                                                                                                                                                                                                                                                                           >  7:760027/1‑140 (MQ=255)
                                                                                                                                        aaGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGt                                                                                                                                                                                                                                                            >  6:214647/1‑149 (MQ=255)
                                                                                                                                           gggaGCGAAGCAGGACCAGGTAGTGCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCAGTCATGTCACATCAGGCATAAGCAAAACGGACGTTGTTAATGTTCGAATGCACGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGTTTCCAGATagcag                                                                                                                           <  2:44903/272‑1 (MQ=255)
                                                                                                                                             tAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCCTAACGCGCACTGGCTTCCTTTAAAAAAGCGGTCGTTTCCAGCTagcagc                                                                                                                          >  4:60196/1‑274 (MQ=255)
                                                                                                                                               gcgcATCAGGCCCAGGTAGTTCCTGCAACCTGGTGGATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCa                                                                                                                                                                                                                                                     <  7:538188/149‑1 (MQ=255)
                                                                                                                                               gcgcATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTACAAAACAGGTCGTTTCCAGATAGCAGCGt                                                                                                                        >  1:46966/1‑274 (MQ=255)
                                                                                                                                                 ggaTCAGGCCCAGATAGTTCCTGCAACCTGGTGAGTTAGTAGCCCGGATAATGCAGTCATGTCACATCCGGCATAAAAAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCAATTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCAGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGTTTCCAGATAGCAGCGTTTg                                                                                                                     <  2:72613/273‑1 (MQ=255)
                                                                                                                                                   aTCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTaga                                                                                                                                                                                                                                                              >  7:1162521/1‑136 (MQ=255)
                                                                                                                                                         cccAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCAc                                                                                                                                                                                                                                           <  6:637429/149‑1 (MQ=255)
                                                                                                                                                         cccAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCAc                                                                                                                                                                                                                                           <  8:101060/149‑1 (MQ=255)
                                                                                                                                                           cAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACtt                                                                                                                                                                                                                                         <  5:1115741/149‑1 (MQ=255)
                                                                                                                                                             ggTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTca                                                                                                                                                                                                                                       <  5:803537/149‑1 (MQ=255)
                                                                                                                                                                  ttCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTGATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATc                                                                                                                                                                                                                                  <  8:209021/149‑1 (MQ=255)
                                                                                                                                                                  ttCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATc                                                                                                                                                                                                                                  >  7:969574/1‑149 (MQ=255)
                                                                                                                                                                  ttCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATc                                                                                                                                                                                                                                  <  5:1162754/149‑1 (MQ=255)
                                                                                                                                                                  ttCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATc                                                                                                                                                                                                                                  >  6:252992/1‑149 (MQ=255)
                                                                                                                                                                  ttCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAATCCCAAATGCTCCTAAAGCGCAATGGCTTCCTTTTAAACAGCGGTCGTTTCCAGATAGCAGCGTTTGAAACCCATCTCTCgcgc                                                                                                    >  2:330545/1‑275 (MQ=255)
                                                                                                                                                                     cTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATc                                                                                                                                                                                                                                  >  8:789654/1‑146 (MQ=255)
                                                                                                                                                                     cTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGAc                                                                                                                                                                                                                               >  6:671893/1‑149 (MQ=255)
                                                                                                                                                                       gCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACAt                                                                                                                                                                                                                             <  5:312654/149‑1 (MQ=255)
                                                                                                                                                                             tGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATc                                                                                                                                                                                                                                  >  8:973171/1‑138 (MQ=255)
                                                                                                                                                                                    tttGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATc                                                                                                                                                                                                                                  >  5:994765/1‑131 (MQ=255)
                                                                                                                                                                                    tttGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATc                                                                                                                                                                                                                                  <  6:994765/131‑1 (MQ=255)
                                                                                                                                                                                      tGTAGCCCGGATAAGGCACTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCaa                                                                                                                                                                                                              <  6:1366000/149‑1 (MQ=255)
                                                                                                                                                                                        tAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAgc                                                                                                                                                                                                            <  8:416528/149‑1 (MQ=255)
                                                                                                                                                                                         aGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAACCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGTTTCCAGATAGCAGCGTTTGAAACCCATCTCTCGCGCCTGCTCCATCGCCATTAAGGcc                                                                              >  4:312946/1‑274 (MQ=255)
                                                                                                                                                                                            ccGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCAt                                                                                                                                                                                                        >  6:1314700/1‑149 (MQ=255)
                                                                                                                                                                                             cGGATAAGGCGTTCATGTCACATCCGGTATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATa                                                                                                                                                                                                       <  7:1055381/149‑1 (MQ=255)
                                                                                                                                                                                               gATAAGGCATTGATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGTCCCGTGCAGCCAAGCGCATAGt                                                                                                                                                                                                     >  7:826100/1‑149 (MQ=255)
                                                                                                                                                                                                aTAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTc                                                                                                                                                                                                    <  7:1201944/149‑1 (MQ=255)
                                                                                                                                                                                                aTAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCAAATGCCCGCGTGTTCAACTCGCGGGCAttc                                                                                                                                                                                                                                                                     >  6:1133468/1‑83 (MQ=37)
                                                                                                                                                                                                 tAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCg                                                                                                                                                                                                   >  5:1217757/1‑149 (MQ=255)
                                                                                                                                                                                                  aaGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGa                                                                                                                                                                                                  <  7:213130/149‑1 (MQ=255)
                                                                                                                                                                                                  aaGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGa                                                                                                                                                                                                  <  6:857952/149‑1 (MQ=255)
                                                                                                                                                                                                  aaGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGCTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGa                                                                                                                                                                                                  >  5:743603/1‑149 (MQ=255)
                                                                                                                                                                                                    ggCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATa                                                                                                                                                                                                       >  7:362497/1‑142 (MQ=255)
                                                                                                                                                                                                    ggCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATa                                                                                                                                                                                                       <  8:362497/142‑1 (MQ=255)
                                                                                                                                                                                                    ggCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGata                                                                                                                                                                                                >  8:1161935/1‑149 (MQ=255)
                                                                                                                                                                                                     gCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATGTTATTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATTGCCCGTGCAGCCAAGCGCATAGTCGatat                                                                                                                                                                                               >  8:1453725/1‑149 (MQ=255)
                                                                                                                                                                                                       aTTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGc                                                                                                                                                                                             >  5:127021/1‑149 (MQ=255)
                                                                                                                                                                                                       aTTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGc                                                                                                                                                                                             >  7:1323072/1‑149 (MQ=255)
                                                                                                                                                                                                           aTGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTaga                                                                                                                                                                                                                                                              >  7:1015749/1‑80 (MQ=255)
                                                                                                                                                                                                           aTGTCACATCCGGCATAAACAAAACGTACGTGGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCaa                                                                                                                                                                                         >  8:1130556/1‑149 (MQ=255)
                                                                                                                                                                                                            tGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCaaa                                                                                                                                                                                        >  5:694129/1‑149 (MQ=255)
                                                                                                                                                                                                             gTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAg                                                                                                                                                                                       >  8:118741/1‑149 (MQ=255)
                                                                                                                                                                                                              tCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTcaca                                                                                                                                                                                                                                     >  6:689204/1‑102 (MQ=255)
                                                                                                                                                                                                              tCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGc                                                                                                                                                                                      >  6:71887/1‑149 (MQ=255)
                                                                                                                                                                                                              tCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGc                                                                                                                                                                                      <  5:966020/149‑1 (MQ=255)
                                                                                                                                                                                                               cacaTCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGcc                                                                                                                                                                                     <  6:560670/149‑1 (MQ=255)
                                                                                                                                                                                                                   tCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGc                                                                                                                                                                                      >  6:411968/1‑144 (MQ=255)
                                                                                                                                                                                                                   tCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGc                                                                                                                                                                                      <  5:411968/144‑1 (MQ=255)
                                                                                                                                                                                                                   tCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCaaa                                                                                                                                                                                 <  6:1412905/149‑1 (MQ=255)
                                                                                                                                                                                                                   tCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCaaa                                                                                                                                                                                 <  6:990587/149‑1 (MQ=255)
                                                                                                                                                                                                                     cGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATg                                                                                                                                                                               >  5:901440/1‑149 (MQ=255)
                                                                                                                                                                                                                        cATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTc                                                                                                                                                                            <  6:1201201/149‑1 (MQ=255)
                                                                                                                                                                                                                         aTAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCa                                                                                                                                                                           <  5:452753/149‑1 (MQ=255)
                                                                                                                                                                                                                                aaaCGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAgcg                                                                                                                                                                    >  7:1369384/1‑149 (MQ=255)
                                                                                                                                                                                                                                    gtacgtTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAAt                                                                                                                                                                <  7:668532/149‑1 (MQ=255)
                                                                                                                                                                                                                                     tacgtTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCaaa                                                                                                                                                                                 <  2:228106/131‑1 (MQ=255)
                                                                                                                                                                                                                                     tacgtTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATg                                                                                                                                                               <  7:1444837/149‑1 (MQ=255)
                                                                                                                                                                                                                                      acggttttAAGGTTCGATGGCCCGGGAGTGTAACACCCGGGCATTGTTTTAGAGTTCGCGCAACAGCCGAACTTCCAAACCGACATGGCCCGTGAAGCAAAGCGCATAGTCGATATGCTAAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGTTTCCAGATAGCAGCGTTTGAAACCCATCTCTCGCGCCTGCTCCATCGCCATTAAGGCCAGTTTTTTTGCCAGCCCTTTGCCGCGGATAGCCGGAAGAAAATAc                                 <  4:306519/268‑1 (MQ=255)
                                                                                                                                                                                                                                        gttgttAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGCGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGat                                                                                                                                                                                                 <  5:52704/112‑1 (MQ=255)
                                                                                                                                                                                                                                        gttgttAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGATATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCAt                                                                                                                                                                          <  7:790016/135‑1 (MQ=255)
                                                                                                                                                                                                                                        gttgttAATGTTCGAATGCACGCGAGTAGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGat                                                                                                                                                                                                 >  6:52704/1‑112 (MQ=255)
                                                                                                                                                                                                                                            ttAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCt                                                                                                                                                        >  5:691412/1‑149 (MQ=255)
                                                                                                                                                                                                                                            ttAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCt                                                                                                                                                        >  8:301050/1‑149 (MQ=255)
                                                                                                                                                                                                                                             tAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACATCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCtt                                                                                                                                                       <  8:826100/149‑1 (MQ=255)
                                                                                                                                                                                                                                              aaTGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCttt                                                                                                                                                      >  7:285245/1‑149 (MQ=255)
                                                                                                                                                                                                                                                 gTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAgc                                                                                                                                                                                                            <  5:23069/92‑1 (MQ=255)
                                                                                                                                                                                                                                                 gTTCGAATGCCCGCGAGTCGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAgc                                                                                                                                                                                                            >  6:23069/1‑92 (MQ=255)
                                                                                                                                                                                                                                                    cGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTaaaaaa                                                                                                                                                <  7:711066/149‑1 (MQ=255)
                                                                                                                                                                                                                                                    cGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTaaaaaa                                                                                                                                                <  8:1369384/149‑1 (MQ=255)
                                                                                                                                                                                                                                                     gAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCAt                                                                                                                                                                          <  7:1154009/122‑1 (MQ=255)
                                                                                                                                                                                                                                                        tGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAGGCGCAATGGCTTCCTTTAAAAAAGCgg                                                                                                                                            <  6:743603/149‑1 (MQ=255)
                                                                                                                                                                                                                                                           ccGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCATATGCTCATGAAGCGCAATGGCTTCCTTTaaaaaaactggcc                                                                                                                                         >  8:873835/1‑142 (MQ=255)
                                                                                                                                                                                                                                                           ccGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAAt                                                                                                                                                                                >  6:1019777/1‑110 (MQ=255)
                                                                                                                                                                                                                                                           ccGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCg                                                                                                                                         >  8:250718/1‑149 (MQ=255)
                                                                                                                                                                                                                                                            cgcgAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGt                                                                                                                                        >  8:476927/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                 gTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGTTTCCa                                                                                                                                   >  8:37549/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                 gTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGTTTCCa                                                                                                                                   >  8:574451/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                 gTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGTTTCCa                                                                                                                                   <  8:8111/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                   tGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGTTTCCAGa                                                                                                                                 <  5:1456100/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                    gAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGTTTCCAGAt                                                                                                                                >  5:885857/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                     aaCACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACCATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGTTTCCAGATAGCAGCGTTTGAAACCCATTTCTCGCGCCTGCTCCATCGCCACTAAGCCCCGTTTTTTTGCCATCCCTTTGCCCCGGATTGCCGGGAGAAAATACATCTTTTGCAGTTCCAAATTATCCGactccc  >  4:85080/1‑272 (MQ=255)
                                                                                                                                                                                                                                                                     aaCACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGTTTCCAGATAGCAGCGTTTGAAACCCATCTCTCGCGCCTGCTCCATCGCCATTAAGGCCAGGTTTTTTGCCAGCCCTTTGCCGCGGGTAGCCGGAAGAAAATACATCTTTTGCAGTTCGCAAGTATCCGactcac  >  2:299766/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                              
AACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGTTTCCAGATAGCAGCGTTTGAAACCCATCTCTCGCGCCTGCTCCATCGCCATTAAGGCCAGTTTTTTTGCCAGCCCTTTGCCGCGGATAGCCGGAAGAAAATACATCTTTTGCAGTTCGCAAATATCCGACTCAC  >  NC_000913/4478756‑4479290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: