Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 770,679 G→T 5.5% intergenic (+68/‑779) mngB → / → cydA alpha‑mannosidase/cytochrome d terminal oxidase, subunit I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913770,6790GT 5.5% 575.5 / 16.1 165intergenic (+68/‑779)mngB/cydAalpha‑mannosidase/cytochrome d terminal oxidase, subunit I
Reads supporting (aligned to +/- strand):  ref base G (57/98);  new base T (4/5);  total (61/104)
Fisher's exact test for biased strand distribution p-value = 7.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01

Only 100 of 167 total aligned reads displayed.

AAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCAGTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCC‑TTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTT  >  NC_000913/770406‑770850
                                                                                                                                                                                                                                                                                  |                                                                                                                                                                            
                    tgaCGGCTTTTTAATCCGGCTGAATCAGCAACCTGTGAGGCGACTGTTGCTTTCAGTCGCGAGGTGATTTCTTGCTCAGAAACGAGTGTGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCC‑TTTTTTACCCGGCCAGTCACGGACGTTCAGGTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTAtt                                                                                                                                                         <  4:50360/273‑1 (MQ=255)
                                      tctgaattgtcAACCTGTGATGTGACTGTTGCTTTCAGTCCGGAGGGTATTTCTTGCTCAGAAACGATGATGGATGAACCCATGACCCCCGAGGAAAATCAAGGTTCGAATCTATCGGGGCC‑TTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGc                                                                                                                                       <  4:204060/265‑1 (MQ=255)
                                       cTGAATTAGCAAACCGGTATGCGACTGTTGCTGTCAGTCGCGAGGTGTTTTTTTGCTCATGAACGATGATGGATGAACACATTACCACCGGGGAAAATCAAGGTTCAAATCTATCGGGGCC‑TTTTTTACCCGGCCAGTCACGGACGTTCAGGTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGc                                                                                                                                       <  4:223563/274‑1 (MQ=255)
                                       cTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCAGTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCC‑TTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATTCCGGATACGGCAGAAACGCTTTGTTCGGTCTACAAAAACAGGCa                                                                                                                                      >  4:120276/1‑275 (MQ=255)
                                                             gACTGTTGCTTTCAGTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCC‑TTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACCTATGCGCGGCCAGATTGTTGAAAAAGGGCGCTTTGTTCATTCCGGGTACGGCATGACAGATTTTTTTGGTCTCCAAAAACCGGCAAATTCAATATAtttcccttat                                                                                                                 >  4:331907/1‑266 (MQ=255)
                                                                      gttCAGTCGGGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTAACACCGAGGAAAAGCAAGTTTCAAATCTCTCGGGGCC‑TTTTTTACCCGGCCAGTCACGGGCGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGaca                                                                                                       <  2:325854/274‑1 (MQ=255)
                                                                            tCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCC‑TTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAATCAGGCCAATTCAATATATTGCAGAGTTGATGTAGACA‑CTTGCa                                                                                                >  2:38651/1‑275 (MQ=255)
                                                                                            tGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCC‑TTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGTGATGATGTA‑ACACCTGGCAAGCGCAGCGCATCAgg                                                                                >  2:293502/1‑275 (MQ=255)
                                                                                              cTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCC‑TTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGc                                                                                                                                           >  2:221271/1‑215 (MQ=255)
                                                                                                  gAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCC‑TTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGc                                                                                                                                       <  4:221271/215‑1 (MQ=255)
                                                                                                       gatcacggatgAACACATTACCACCGAGGAAAATTAATGTTCAAATCTAGCGGGGCC‑TTTTTTACCCGGCCAGTCACGGACGTTAAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGCg                                                                      <  2:243870/268‑1 (MQ=255)
                                                                                                        atgatgGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCC‑TTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAATGGCGCTTTGTTAATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGGAGCGCAACAGGGAAtttt                                                                         >  4:243870/1‑270 (MQ=255)
                                                                                                             ggatgAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCC‑TTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATc                                                                >  4:45117/1‑274 (MQ=255)
                                                                                                              gatgAACACATGACCACCGAGAAAAAACAAGGTTCAAACCTATCGTGGCC‑TTTTTTACCCGGCCAGTCACGGACTTTCAGTTACCGGCTTTCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCtt                                                              <  4:158094/275‑1 (MQ=255)
                                                                                                                   acacATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACa                                                                                                                                                >  4:336143/1‑190 (MQ=255)
                                                                                                                       aTTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAg                                                                                                                                            <  2:336143/190‑1 (MQ=255)
                                                                                                                              ccGAGGAAAATCAAGGTTCAAATCTATCGGGGCC‑TTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGa                                                                                                                                                                             >  5:149324/1‑149 (MQ=255)
                                                                                                                                       aTCAAGGTTCAAATCTATCGGGGCC‑TTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATg                                                                                                                                                                    >  5:393451/1‑149 (MQ=255)
                                                                                                                                          aaGGTTCAAATCTATCGGGGCC‑TTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCAt                                                                                                                                                                     >  7:646314/1‑145 (MQ=255)
                                                                                                                                          aaGGTTCAAATCTATCGGGGCC‑TTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCAt                                                                                                                                                                     <  8:646314/145‑1 (MQ=255)
                                                                                                                                           aGGTTCAAATCTATCGGGGCC‑TTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACg                                                                                                                                                                >  8:378325/1‑149 (MQ=255)
                                                                                                                                            ggTTCAAATCTATCGGGGCC‑TTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGc                                                                                                                                                               <  6:1028177/149‑1 (MQ=255)
                                                                                                                                             gTTCAAATCTATCGGGGCC‑TTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGTATCCGGCATGAAcaaa                                                                                                                                                              <  5:90405/149‑4 (MQ=255)
                                                                                                                                             gTTCAAATCTATCGGGGCC‑TTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGTATCCGGCATGAAcaaa                                                                                                                                                              <  7:1237521/149‑4 (MQ=255)
                                                                                                                                             gTTCAAATCTATCGGGGCC‑TTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCt                                                                                                                                                              <  5:216795/149‑1 (MQ=255)
                                                                                                                                             gTTCAAATCTATCGGGGCC‑TTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCt                                                                                                                                                              <  6:845715/149‑1 (MQ=255)
                                                                                                                                              ttCAAATCTATCGGGGCC‑TTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCtt                                                                                                                                                             <  7:395473/149‑1 (MQ=255)
                                                                                                                                                     cTATCGGGGCC‑TTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCgg                                                                                                                                                      <  8:1402952/149‑1 (MQ=255)
                                                                                                                                                           gggCC‑TTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACa                                                                                                                                                >  7:184546/1‑149 (MQ=255)
                                                                                                                                                            ggCC‑TTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAaataat                 <  2:120276/274‑1 (MQ=255)
                                                                                                                                                              cc‑TTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACaaaa                                                                                                                                             >  5:894571/1‑149 (MQ=255)
                                                                                                                                                                 ttttttACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGc                                                                                                                                           >  5:315993/1‑149 (MQ=255)
                                                                                                                                                                  tttttACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCa                                                                                                                                          <  8:184546/149‑1 (MQ=255)
                                                                                                                                                                          cGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAAtt                                                                                                                                  <  7:152169/149‑1 (MQ=255)
                                                                                                                                                                               aGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATa                                                                                                                                                                           <  4:64095/103‑1 (MQ=255)
                                                                                                                                                                                 tCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGGTACGGCATGAACGCTTTATTCGGTCTACAAAAGCAg                                                                                                                                         <  8:1381351/135‑1 (MQ=255)
                                                                                                                                                                                 tCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAg                                                                                                                                         >  7:1381351/1‑135 (MQ=255)
                                                                                                                                                                                 tCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAatata                                                                                                                           >  7:871752/1‑149 (MQ=255)
                                                                                                                                                                                      gACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCa                                                                                                                      <  5:552209/149‑1 (MQ=255)
                                                                                                                                                                                       aCGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATAtt                                                                                                                         >  1:71327/1‑145 (MQ=255)
                                                                                                                                                                                        cGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCaga                                                                                                                    <  8:33867/149‑1 (MQ=255)
                                                                                                                                                                                         gTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCagag                                                                                                                   >  7:1191548/1‑149 (MQ=255)
                                                                                                                                                                                           tCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGc                                                                                                                                       >  6:722738/1‑127 (MQ=255)
                                                                                                                                                                                           tCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGc                                                                                                                                       <  5:722738/127‑1 (MQ=255)
                                                                                                                                                                                           tCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCaa                                                                                                                               >  8:422009/1‑135 (MQ=255)
                                                                                                                                                                                           tCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCaa                                                                                                                               <  7:422009/135‑1 (MQ=255)
                                                                                                                                                                                           tCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCaa                                                                                                                               <  7:912545/135‑1 (MQ=255)
                                                                                                                                                                                           tCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCag                                                                                                                     <  3:71327/145‑1 (MQ=255)
                                                                                                                                                                                           tCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAgat                                                                                                                 <  7:1080920/149‑1 (MQ=255)
                                                                                                                                                                                              gTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAgatgat                                                                                                              <  8:869753/149‑1 (MQ=255)
                                                                                                                                                                                               ttACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATAAGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGatgatg                                                                                                             <  8:1191548/149‑1 (MQ=255)
                                                                                                                                                                                               ttACCGGCTTGCCGGAATAGCAATCAAAGCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATGCGGCATGAACGCTTTATTCGGGCTACAAAACCAGGCAAATGGAATATAGTGGagaggggaag                                                                                                             >  6:504198/1‑143 (MQ=25)
                                                                                                                                                                                                tACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGt                                                                                                            <  8:715556/149‑1 (MQ=255)
                                                                                                                                                                                                 aCCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAgat                                                                                                                 >  5:1160933/1‑143 (MQ=255)
                                                                                                                                                                                                 aCCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTa                                                                                                           <  6:809598/149‑1 (MQ=255)
                                                                                                                                                                                                  ccGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAgatga                                                                                                               >  7:202079/1‑144 (MQ=255)
                                                                                                                                                                                                  ccGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAgatga                                                                                                               <  8:202079/144‑1 (MQ=255)
                                                                                                                                                                                                   cGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGa                                                                                                         >  8:990025/1‑149 (MQ=255)
                                                                                                                                                                                                   cGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGa                                                                                                         <  5:61565/149‑1 (MQ=255)
                                                                                                                                                                                                     gCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGaca                                                                                                       >  5:376042/1‑149 (MQ=255)
                                                                                                                                                                                                           cTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCa                                                                                                >  7:1105311/1‑149 (MQ=255)
                                                                                                                                                                                                            tGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCaa                                                                                               >  8:1030008/1‑149 (MQ=255)
                                                                                                                                                                                                             gAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACGAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAAGCAAATTCAATATATTGCAGAGATGATGTAGACA‑ATGGCAAGCGTAGCGCATCAGGTAAGTTTGAGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGt       <  2:99186/236‑1 (MQ=255)
                                                                                                                                                                                                                 aGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTa                                                                                          <  8:1440324/149‑1 (MQ=255)
                                                                                                                                                                                                                  gCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAg                                                                                         <  6:1478225/149‑1 (MQ=255)
                                                                                                                                                                                                                   cAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAgg                                                                                                                                        <  5:1228070/102‑1 (MQ=255)
                                                                                                                                                                                                                   cAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAgg                                                                                                                                        >  6:1228070/1‑102 (MQ=255)
                                                                                                                                                                                                                     aTCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACaaaa                                                                                                                                             >  8:210337/1‑95 (MQ=255)
                                                                                                                                                                                                                      tCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCt                                                                                                                                                   >  8:1226886/1‑88 (MQ=255)
                                                                                                                                                                                                                       cAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAatatat                                                                                                                          <  7:990091/112‑1 (MQ=255)
                                                                                                                                                                                                                        aaaCCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGaca‑c                                                                                                     >  2:381829/1‑131 (MQ=255)
                                                                                                                                                                                                                         aaCCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCt                                                                                                                                                              >  6:1125100/1‑74 (MQ=255)
                                                                                                                                                                                                                          aCCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAg                                                                                 <  5:997183/149‑1 (MQ=255)
                                                                                                                                                                                                                            cGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGc                                                                                                 <  4:381828/131‑1 (MQ=255)
                                                                                                                                                                                                                                  cacaTATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAAtttt                                                                         >  7:256359/1‑149 (MQ=255)
                                                                                                                                                                                                                                  cacaTATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAAtttt                                                                         <  8:1105311/149‑1 (MQ=255)
                                                                                                                                                                                                                                    caTATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGc                                                                       <  7:33923/149‑1 (MQ=255)
                                                                                                                                                                                                                                     atatGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACTGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGCg                                                                      <  5:504198/149‑1 (MQ=255)
                                                                                                                                                                                                                                      tatGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGCGt                                                                     <  8:256359/149‑1 (MQ=255)
                                                                                                                                                                                                                                             ggCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCtt                                                              >  8:438044/1‑149 (MQ=255)
                                                                                                                                                                                                                                                      gttgACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCaa                                                     <  8:452111/149‑1 (MQ=255)
                                                                                                                                                                                                                                                       ttgACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAg                                                    <  6:582234/149‑1 (MQ=255)
                                                                                                                                                                                                                                                       ttgACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTa                           >  2:191101/1‑174 (MQ=255)
                                                                                                                                                                                                                                                              aGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCAc                                                <  5:451511/146‑1 (MQ=255)
                                                                                                                                                                                                                                                              aGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCAc                                                >  6:451511/1‑146 (MQ=255)
                                                                                                                                                                                                                                                                 gcgcTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTatat                                          <  5:353833/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                       tGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCt                                    <  5:777826/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                       tGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCt                                    <  6:191682/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                        gTTCATGCCGTATCCGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCtt                                   >  8:1037335/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                        gTTCATGCCGTATCCGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCtt                                   >  7:40063/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                          tCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTg                                                                        >  3:53565/1‑110 (MQ=255)
                                                                                                                                                                                                                                                                            aTGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAACGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACATATACGTGGCttgaga                               <  7:1277452/149‑5 (MQ=255)
                                                                                                                                                                                                                                                                            aTGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACATATACGTGGCttgaga                               <  5:801965/149‑5 (MQ=255)
                                                                                                                                                                                                                                                                            aTGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACATATACGTGGCttgaga                               <  6:315993/149‑5 (MQ=255)
                                                                                                                                                                                                                                                                              gCCGTATCCGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTAttt                              >  5:943015/1‑148 (MQ=255)
                                                                                                                                                                                                                                                                              gCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGCGtt                                                                    <  1:53565/110‑1 (MQ=255)
                                                                                                                                                                                                                                                                              gCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAAcc             <  2:60238/165‑1 (MQ=255)
                                                                                                                                                                                                                                                                               ccGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTAttttt                            >  6:251256/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                               ccGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTAttttt                            <  6:376042/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 ggATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTaa                          <  8:29096/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  |                                                                                                                                                                            
AAGACCGACAAGAAGTGATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCAGTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCC‑TTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACA‑CTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTT  >  NC_000913/770406‑770850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑