Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 847,213 A→T 9.9% intergenic (‑94/+45) glnP ← / ← glnH glutamine transporter subunit/glutamine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913847,2130AT 9.9% 695.7 / 54.5 225intergenic (‑94/+45)glnP/glnHglutamine transporter subunit/glutamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (107/90);  new base T (10/12);  total (120/105)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.26e-01

Only 100 of 226 total aligned reads displayed.

ACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTACCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGC  >  NC_000913/846950‑847482
                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                             
aCCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTACCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGNCCGACCCGATGGCACTCCATGCAAACTGCATATGTTGTTCCGGTTACCGTGGTGTCCATATTCACATTAAGGGTAATTAANGGCCCCGGAAGGGGTTTGAGCCCCTNACCTCCCTCCTCCCCCCCCAGGGGGGAgg                                                                                                                                                                                                                                                                    >  3:62759/1‑275 (MQ=255)
                                                    ccAGCAATCCGATTACCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGCGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGcc                                                                                                                                                                                                                                                            >  4:142431/1‑231 (MQ=255)
                                                        cAATCCGATTACCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAAGAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTg                                                                                                                                                                                                                                                        <  2:142431/231‑1 (MQ=255)
                                                                                                                    aCCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCAt                                                                                                                                                                                                                                                                              >  5:526904/1‑149 (MQ=255)
                                                                                                                      cTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAg                                                                                                                                                                                                                                                                            <  6:301961/149‑1 (MQ=255)
                                                                                                                       ttCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAgg                                                                                                                                                                                                                                                                           <  7:689079/149‑1 (MQ=255)
                                                                                                                       ttCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAgg                                                                                                                                                                                                                                                                           >  5:1470443/1‑149 (MQ=255)
                                                                                                                       ttCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAgg                                                                                                                                                                                                                                                                           >  8:689079/1‑149 (MQ=255)
                                                                                                                        tCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGttatt                                                                                                                                                                                                                                 >  4:373636/1‑190 (MQ=255)
                                                                                                                           aTCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCTATGGGgag                                                                                                                                                                                                                                                                       >  7:616379/1‑149 (MQ=255)
                                                                                                                            tCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAATCTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCGCATAGGGgaga                                                                                                                                                                                                                                                                      <  7:1280090/149‑1 (MQ=255)
                                                                                                                            tCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGgaga                                                                                                                                                                                                                                                                      <  5:1255065/149‑1 (MQ=255)
                                                                                                                            tCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTAttt                                                                                                                                                                                                                             <  2:373637/190‑1 (MQ=255)
                                                                                                                            tCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCCGGTTTGCGCCCCTCACCCTAATCCTCTCCCCTATGGGgaga                                                                                                                                                                                                                                                                      <  8:87990/149‑1 (MQ=255)
                                                                                                                             cAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGagag                                                                                                                                                                                                                                                                     <  8:174044/149‑1 (MQ=255)
                                                                                                                             cAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGagag                                                                                                                                                                                                                                                                     <  7:923310/149‑1 (MQ=255)
                                                                                                                               gAAGCGGAATGGCAGGCCATATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGTTGTAAATAATCAAATTCAGGGTAAGTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAAACCTCTCCCCGATGGGGAGAgga                                                                                                                                                                                                                                                                   <  8:1124181/149‑2 (MQ=255)
                                                                                                                                aaGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGa                                                                                                                                                                                                                                                                  >  5:898885/1‑149 (MQ=255)
                                                                                                                                 aGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGaa                                                                                                                                                                                                                                                                 <  7:1008633/149‑1 (MQ=255)
                                                                                                                                  gCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCTATGGGGAGAggatta                                                                                                                                                                                                                                                                <  6:1084817/149‑5 (MQ=255)
                                                                                                                                    ggAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAACCCTCTCCCCTATGGGGAGAggattagg                                                                                                                                                                                                                                                              <  5:67215/149‑7 (MQ=255)
                                                                                                                                      aaTGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGcc                                                                                                                                                                                                                                                            >  7:780689/1‑149 (MQ=255)
                                                                                                                                           cAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCTATGGGGAGAgga                                                                                                                                                                                                                                                                   <  6:98226/137‑2 (MQ=255)
                                                                                                                                           cAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCTATGGGGAGAgga                                                                                                                                                                                                                                                                   >  5:98226/1‑136 (MQ=255)
                                                                                                                                              gCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGtt                                                                                                                                                                                                                                                    >  7:629903/1‑149 (MQ=255)
                                                                                                                                                cAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTGATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAATTTCCGTTCTCGCGCAGGGTTTTCAACGCTCCGTTGACTTTGTCACGCAGCTCGTCGCTACCtt                                                                                                                     >  2:95379/1‑274 (MQ=255)
                                                                                                                                                    tGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAg                                                                                                                                                                                                                                              <  5:832645/149‑1 (MQ=255)
                                                                                                                                                      gCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGgt                                                                                                                                                                                                                                            <  7:247582/149‑1 (MQ=255)
                                                                                                                                                         cTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTa                                                                                                                                                                                                                                         >  7:500083/1‑149 (MQ=255)
                                                                                                                                                          tCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGga                                                                                                                                                                                                                                                                        <  5:37262/117‑1 (MQ=255)
                                                                                                                                                          tCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAg                                                                                                                                                                                                                                        >  6:427139/1‑149 (MQ=255)
                                                                                                                                                             aGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGt                                                                                                                                                                                                                                     >  8:856511/1‑149 (MQ=255)
                                                                                                                                                               tCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGtta                                                                                                                                                                                                                                   >  7:571181/1‑149 (MQ=255)
                                                                                                                                                                 aaaCTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCTCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGttatt                                                                                                                                                                                                                                 >  8:790341/1‑149 (MQ=255)
                                                                                                                                                                 aaaCTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTGCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGttatt                                                                                                                                                                                                                                 >  6:894731/1‑149 (MQ=255)
                                                                                                                                                                     tGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTAttt                                                                                                                                                                                                                             >  7:808332/1‑149 (MQ=255)
                                                                                                                                                                     tGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTAttt                                                                                                                                                                                                                             <  5:578942/149‑1 (MQ=255)
                                                                                                                                                                      gCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTc                                                                                                                                                                                                                            <  8:313216/149‑1 (MQ=255)
                                                                                                                                                                      gCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTc                                                                                                                                                                                                                            <  8:983652/149‑1 (MQ=255)
                                                                                                                                                                      gCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGATTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTc                                                                                                                                                                                                                            <  7:98418/149‑1 (MQ=255)
                                                                                                                                                                       cATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCCTAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCCGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTTTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCTTTGACTTTGTCCCGCAGCCCGTCGCTACCTTTCCGGTACGCAATACCGTATtgct                                                                                             >  2:310615/1‑275 (MQ=255)
                                                                                                                                                                        atatGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGaa                                                                                                                                                                                                                                                                 >  7:1088241/1‑110 (MQ=255)
                                                                                                                                                                        atatGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGaa                                                                                                                                                                                                                                                                 <  8:1088241/110‑1 (MQ=255)
                                                                                                                                                                          gttgtgttACTGTTAACGTTGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGGTTGCGCCCCTCACCCTAATACTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTAGTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTgcg                                                                                          <  1:62759/271‑1 (MQ=255)
                                                                                                                                                                          atGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGgt                                                                                                                                                                                                                                            >  5:599111/1‑129 (MQ=255)
                                                                                                                                                                          atGCTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGtt                                                                                                                                                                                                                        <  6:83788/149‑1 (MQ=255)
                                                                                                                                                                                ttCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTAc                                                                                                                                                                                                                  >  8:6756/1‑149 (MQ=255)
                                                                                                                                                                                ttCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTAc                                                                                                                                                                                                                  >  8:5821/1‑149 (MQ=255)
                                                                                                                                                                                   cTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGt                                                                                                                                                                                                                                                         >  6:768700/1‑107 (MQ=255)
                                                                                                                                                                                   cTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGt                                                                                                                                                                                                                                                         <  5:768700/107‑1 (MQ=255)
                                                                                                                                                                                    tGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGaa                                                                                                                                                                                                              >  7:486852/1‑149 (MQ=255)
                                                                                                                                                                                          cGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCAttt                                                                                                                                                                                                        >  5:1333570/1‑149 (MQ=255)
                                                                                                                                                                                              gtgtAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTg                                                                                                                                                                                                    >  6:1017113/1‑149 (MQ=255)
                                                                                                                                                                                              gtgtAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTtc                                                                                                                                                                            >  4:190910/1‑173 (MQ=255)
                                                                                                                                                                                               tgtAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGt                                                                                                                                                                                                   >  5:841640/1‑149 (MQ=255)
                                                                                                                                                                                               tgtAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCGCCCCATAGGGGAGAGGGACCTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTACGGTTCAGTACCGAACCATTCTTTGt                                                                                                                                                                                                   <  8:927669/149‑1 (MQ=255)
                                                                                                                                                                                                gtAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTa                                                                                                                                                                                                  <  6:365972/149‑1 (MQ=255)
                                                                                                                                                                                                 tAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGGGCGTTTTACAGTTTTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAg                                                                                                                                                                                                 >  6:223747/1‑149 (MQ=255)
                                                                                                                                                                                                  aaaTAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGa                                                                                                                                                                                                <  8:822093/149‑1 (MQ=255)
                                                                                                                                                                                                       atCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCg                                                                                                                                                                                           >  7:1164411/1‑149 (MQ=255)
                                                                                                                                                                                                       atCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCg                                                                                                                                                                                           >  7:387531/1‑149 (MQ=255)
                                                                                                                                                                                                        tCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGt                                                                                                                                                                                          <  5:272973/149‑1 (MQ=255)
                                                                                                                                                                                                         cAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGtt                                                                                                                                                                                         <  8:241002/149‑1 (MQ=255)
                                                                                                                                                                                                             ttCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTaa                                                                                                                                                                                     >  5:36349/1‑149 (MQ=255)
                                                                                                                                                                                                                 gggTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTc                                                                                                                                                                                 <  7:1442098/149‑1 (MQ=255)
                                                                                                                                                                                                                  ggTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAAcgcg                                                                                                                                                       >  4:183649/1‑174 (MQ=255)
                                                                                                                                                                                                                   gTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCg                                                                                                                                                                               >  6:1338940/1‑149 (MQ=255)
                                                                                                                                                                                                                    taattaatGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGt                                                                                                                                                                              >  8:1038039/1‑149 (MQ=255)
                                                                                                                                                                                                                        taatGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTtctc                                                                                                                                                                          <  5:776573/149‑1 (MQ=255)
                                                                                                                                                                                                                         aatGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTcg                                                                                                                                                                         >  5:214010/1‑149 (MQ=255)
                                                                                                                                                                                                                          atGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTcgc                                                                                                                                                                        >  6:779793/1‑149 (MQ=255)
                                                                                                                                                                                                                            ggCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCgcgc                                                                                                                                                                      >  5:1263507/1‑149 (MQ=255)
                                                                                                                                                                                                                                ccGGGGCGGGTTTGCTCCCCTCCCCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTtgatg                                     <  4:95379/274‑1 (MQ=255)
                                                                                                                                                                                                                                 cGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTtgatga                                    <  4:252922/274‑1 (MQ=255)
                                                                                                                                                                                                                                   gAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGttt                                                                                                                                                               <  6:1263505/149‑1 (MQ=255)
                                                                                                                                                                                                                                    aGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGtttt                                                                                                                                                              <  7:1185967/149‑1 (MQ=255)
                                                                                                                                                                                                                                     gCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTc                                                                                                                                                             >  5:440776/1‑149 (MQ=255)
                                                                                                                                                                                                                                        ggTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATCTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAAc                                                                                                                                                          <  5:1065753/149‑1 (MQ=255)
                                                                                                                                                                                                                                                ccccTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGtt                                                                                                                                                  <  6:869999/149‑1 (MQ=255)
                                                                                                                                                                                                                                                 cccTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTg                                                                                                                                                 >  6:1473661/1‑149 (MQ=255)
                                                                                                                                                                                                                                                 cccTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTg                                                                                                                                                 <  7:856511/149‑1 (MQ=255)
                                                                                                                                                                                                                                                 cccTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTg                                                                                                                                                 >  7:1384355/1‑149 (MQ=255)
                                                                                                                                                                                                                                                  ccTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGa                                                                                                                                                <  7:1038962/149‑1 (MQ=255)
                                                                                                                                                                                                                                                   cTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGTTGAAGTCAAGAATGTTTGGAGt                 >  2:246323/1‑275 (MQ=255)
                                                                                                                                                                                                                                                   cTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTTCAGAATGTTTGGCg                  >  2:163628/1‑274 (MQ=255)
                                                                                                                                                                                                                                                      aCCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACttt                                                                                                                                            <  7:210952/149‑1 (MQ=255)
                                                                                                                                                                                                                                                        ccTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGt                                                                                                                                          >  6:296174/1‑149 (MQ=255)
                                                                                                                                                                                                                                                         cTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTc                                                                                                                                         <  8:361946/149‑1 (MQ=255)
                                                                                                                                                                                                                                                           ttcccTCTCCCCTATGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCAc                                                                                                                                       >  5:1032777/4‑149 (MQ=255)
                                                                                                                                                                                                                                                              ccTCTCCCCTATGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGt                                                                                                                                                                                          <  8:1154338/95‑1 (MQ=255)
                                                                                                                                                                                                                                                              ccTCTCCCCTATGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAAc                                                                                                                                                          <  7:998617/127‑1 (MQ=255)
                                                                                                                                                                                                                                                              ccTCTCCCCTATGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGtt                                                                                                                                                  <  6:748848/135‑1 (MQ=255)
                                                                                                                                                                                                                                                               ctctCCCCTATGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCa                                                                                                                                                                     >  7:1334985/1‑115 (MQ=255)
                                                                                                                                                                                                                                                               ctctCCCCTATGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCa                                                                                                                                        >  6:532349/1‑144 (MQ=255)
                                                                                                                                                                                                                                                               ctctCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAg                                                                                                                                   >  8:951020/1‑149 (MQ=255)
                                                                                                                                                                                                                                                               ctctCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAg                                                                                                                                   >  8:46209/1‑149 (MQ=255)
                                                                                                                                                                                                                                                               ctctCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAg                                                                                                                                   >  5:283165/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                     ccATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCtcgtc                                                                                                                             <  7:6756/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                     ccATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCtcgtc                                                                                                                             >  8:1370597/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                      cATAGGGGAGAGGGAACTGCCAGTTCTTTTTACAGGTGTAGCGTTATTATTGCGGTTCAGTACCGAACCATTTTTTGTAGATGGCGTTGTAAGTTCCGTTCGCGCGCAGGGTTTTCAACGCGCCGTTGACTTTCGCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTTCAGCGAGTCACCTa                                                                        <  1:2463/201‑1 (MQ=255)
                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                             
ACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTACCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGC  >  NC_000913/846950‑847482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑