BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	915,080	G→C	9.1%	intergenic (‑223/+272)	ybjE ← / ← aqpZ	putative transporter/aquaporin Z

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	915,080	0	G	C	9.1%	438.4 / 30.0	132	intergenic (‑223/+272)	ybjE/aqpZ	putative transporter/aquaporin Z
Reads supporting (aligned to +/- strand): ref base G (46/74); new base C (6/6); total (52/80)
Fisher's exact test for biased strand distribution p-value = 5.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.13e-01

Only 100 of 133 total aligned reads displayed.

CCCACAATCAGGGGAACCAGAATGATTAACAGCCCAGAAAACATGAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTT  >  NC_000913/914814‑915349

cccACAATCAGGGGAACCAGAATGATTAACAGCCCAGAAAACATGAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTCCCCCGATGTTGGTGGCCTCAAAATCCCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACCTGGGTGTggg                                                                                                                                                                                                                                                                       >  4:17334/1‑275 (MQ=255)

     aaTCAGGGGAACCAGAATGATTAACAGCCCAGAAAACATGAAAACCCAGTCCTTGCAAAGATGAAGTCGATATGCGCGATGACACACTACTGAAAGCGGACGGACGAGCAAAGTTGCCATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCTCCAAAATCGCCGAGGCGTCCCCTGAAGCCCGGGGTAGCCTACATGTAtgtgtgtggag                                                                                                                                                                                                                                                                  >  1:5369/1‑268 (MQ=255)

                                                            cAAAGATGAAGTCGAAATGAGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGAAGCCCATATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGAt                                                                                                                                                                                                             <  2:86837/273‑1 (MQ=255)

                                                                      gTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCa                                                                                                                                                                                                 <  2:19454/275‑1 (MQ=255)

                                                                                                        aaGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGACCCGCCCCGCGGCGCTTTTGTTCGCCAGGGCCCgg                                                                                                                                                               >  4:38938/1‑275 (MQ=255)

                                                                                                                      ttGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATg                                                                                                                                                                                                                                                                               >  6:943063/1‑149 (MQ=255)

                                                                                                                      ttGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGAcc                                                                                                                                                                                                                              >  2:35693/1‑198 (MQ=255)

                                                                                                                         cAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGAt                                                                                                                                                                                                                                                                            <  7:1065128/149‑1 (MQ=255)

                                                                                                                         cAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGAt                                                                                                                                                                                                                                                                            <  6:1305386/149‑1 (MQ=255)

                                                                                                                          aaTTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGGTGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGAtg                                                                                                                                                                                                                                                                           >  5:895251/1‑149 (MQ=255)

                                                                                                                          aaTTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGt                                                                                                                                                                                                                          <  4:35693/198‑1 (MQ=255)

                                                                                                                           aTTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGAtgt                                                                                                                                                                                                                                                                          >  6:646333/1‑149 (MQ=255)

                                                                                                                             tAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTgg                                                                                                                                                                                                                                                                        <  6:713632/149‑1 (MQ=255)

                                                                                                                             tAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTgg                                                                                                                                                                                                                                                                        <  5:943063/149‑1 (MQ=255)

                                                                                                                                aaGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTGATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCt                                                                                                                                                                                                                                                                     >  8:841487/1‑149 (MQ=255)

                                                                                                                                aaGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTgg                                                                                                                                                                                                                                                                        >  6:847893/1‑146 (MQ=255)

                                                                                                                                   gAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAg                                                                                                                                                                                                                                                                  <  8:766023/149‑1 (MQ=255)

                                                                                                                                   gAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAg                                                                                                                                                                                                                                                                  <  5:684361/149‑1 (MQ=255)

                                                                                                                                        gTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGgcgc                                                                                                                                                                                                                                                             <  5:590984/149‑1 (MQ=255)

                                                                                                                                          tATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGt                                                                                                                                                                                                                                                           <  7:725194/149‑1 (MQ=255)

                                                                                                                                           aTGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGGGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGtt                                                                                                                                                                                                                                                          <  8:383782/149‑1 (MQ=255)

                                                                                                                                            tGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGttt                                                                                                                                                                                                                                                         <  6:355101/149‑1 (MQ=255)

                                                                                                                                             gCATAAGGAGCAGTAGAGTATTCGTTTTCATTTGAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTgg                                                                                                                                                                                                                                                                        >  5:385937/1‑133 (MQ=255)

                                                                                                                                             gCATAAGGAGCAGTAGAGTATTCGTTTTCATTTGAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTgg                                                                                                                                                                                                                                                                        <  6:385937/133‑1 (MQ=255)

                                                                                                                                              cATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTgcgc                                                                                                                         >  2:93675/1‑275 (MQ=255)

                                                                                                                                               aTAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTAc                                                                                                                                                                                                                                                      <  5:484162/149‑1 (MQ=255)

                                                                                                                                               aTAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTAc                                                                                                                                                                                                                                                      <  5:646333/149‑1 (MQ=255)

                                                                                                                                               aTAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTAc                                                                                                                                                                                                                                                      <  6:829516/149‑1 (MQ=255)

                                                                                                                                                tAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACa                                                                                                                                                                                                                                                     <  8:783585/149‑1 (MQ=255)

                                                                                                                                                  aGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAgg                                                                                                                                                                                                                                                   <  7:882327/149‑1 (MQ=255)

                                                                                                                                                     aGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGAt                                                                                                                                                                                                                                                <  8:742285/149‑1 (MQ=255)

                                                                                                                                                     aGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGAt                                                                                                                                                                                                                                                <  7:1104678/149‑1 (MQ=255)

                                                                                                                                                     aGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGAt                                                                                                                                                                                                                                                <  7:275627/149‑1 (MQ=255)

                                                                                                                                                     aGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGAt                                                                                                                                                                                                                                                <  7:99282/149‑1 (MQ=255)

                                                                                                                                                        aGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTTCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCCCTCCTTgcc                                                                                                               >  2:55227/1‑274 (MQ=255)

                                                                                                                                                                aTTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTcgcg                                                                                                                                                                                                                                     >  6:923624/1‑149 (MQ=255)

                                                                                                                                                                 ttCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTTAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGCAAGGGATAAGTCGAGGGCACCGCGCAGCGGAGATTTTGTTCGCCATAGCCCGGGGGTTCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTGCTTGCGCCAGTGGc                                                                                                       >  2:251873/1‑274 (MQ=255)

                                                                                                                                                                 ttCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTc                                                                                                                                                                                                                                        >  6:97799/1‑145 (MQ=255)

                                                                                                                                                                 ttCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTc                                                                                                                                                                                                                                        <  5:97799/145‑1 (MQ=255)

                                                                                                                                                                      tttCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGAc                                                                                                                                                                                                                               <  7:876544/149‑1 (MQ=255)

                                                                                                                                                                         cATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCg                                                                                                                                                                                                                            <  5:767637/149‑1 (MQ=255)

                                                                                                                                                                          aTTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATCCATGTGGGc                                                                                                                                                                                                                                                                      <  6:1366925/106‑1 (MQ=255)

                                                                                                                                                                          aTTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATCCATGTGGGc                                                                                                                                                                                                                                                                      >  5:1366925/1‑106 (MQ=255)

                                                                                                                                                                           tttAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGt                                                                                                                                                                                                                          >  7:1169089/1‑149 (MQ=255)

                                                                                                                                                                           tttAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGt                                                                                                                                                                                                                          >  8:678043/1‑149 (MQ=255)

                                                                                                                                                                            ttAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTa                                                                                                                                                                                                                         >  6:485142/1‑149 (MQ=255)

                                                                                                                                                                            ttAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTa                                                                                                                                                                                                                         <  6:967722/149‑1 (MQ=255)

                                                                                                                                                                              aaaGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGc                                                                                                                                                                                                                       >  6:743816/1‑149 (MQ=255)

                                                                                                                                                                                aGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCg                                                                                                                                                                                                                     >  5:846245/1‑149 (MQ=255)

                                                                                                                                                                                aGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATCCATGTGGGc                                                                                                                                                                                                                                                                      >  6:1050686/1‑100 (MQ=255)

                                                                                                                                                                                aGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATCCATGTGGGc                                                                                                                                                                                                                                                                      <  5:1050686/100‑1 (MQ=255)

                                                                                                                                                                                   tatTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTaa                                                                                                                                                                                                                  <  7:841487/149‑1 (MQ=255)

                                                                                                                                                                                   tatTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTaa                                                                                                                                                                                                                  >  7:1375968/1‑149 (MQ=255)

                                                                                                                                                                                    atTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGAGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGGGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCACCCTGCGCGCCCCTTGCGCCCGGGGCAATATGTTACTGACCGCATg                                                                                   >  4:310254/1‑275 (MQ=17)

                                                                                                                                                                                        ttGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGAt                                                                                                                                                                                                             <  7:903108/149‑1 (MQ=255)

                                                                                                                                                                                          gcgcTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGa                                                                             >  2:143177/1‑275 (MQ=255)

                                                                                                                                                                                           cgcTTTAAGTACAAACTGCACAGATGTTGGTGGCGGCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCCCATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAg                                                                            <  2:17334/275‑1 (MQ=255)

                                                                                                                                                                                            gcTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAggg                                                                                                                                                                                                                                                  >  5:137031/1‑108 (MQ=255)

                                                                                                                                                                                               ttAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGa                                                                                                                                                                                                      <  8:1169089/149‑1 (MQ=255)

                                                                                                                                                                                                tAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAg                                                                                                                                                                                                     <  6:689374/149‑1 (MQ=255)

                                                                                                                                                                                                tAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAg                                                                                                                                                                                                     <  7:678043/149‑1 (MQ=255)

                                                                                                                                                                                                tAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATGGGCCGCCCCCGACGCGCTCCTTGCTCAAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGTGcaa                                                                       >  2:255232/1‑275 (MQ=255)

                                                                                                                                                                                                 aaTTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAgg                                                                                                                                                                                                    <  7:166841/149‑1 (MQ=255)

                                                                                                                                                                                                   ttACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGAtgtg                                                                                                                                                                                                                                                                         <  6:525883/78‑1 (MQ=255)

                                                                                                                                                                                                   ttACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGAtgtg                                                                                                                                                                                                                                                                         >  5:525883/1‑78 (MQ=255)

                                                                                                                                                                                                   ttACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGAt                                                                                                                                                                                                                                                <  6:651030/103‑1 (MQ=255)

                                                                                                                                                                                                   ttACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGAt                                                                                                                                                                                                                                                >  5:651030/1‑103 (MQ=255)

                                                                                                                                                                                                   ttACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGc                                                                                                                                                                                                  >  5:861973/1‑149 (MQ=255)

                                                                                                                                                                                                   ttACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGc                                                                                                                                                                                                  >  7:13945/1‑149 (MQ=255)

                                                                                                                                                                                                   ttACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGc                                                                                                                                                                                                  >  6:240644/1‑149 (MQ=255)

                                                                                                                                                                                                       aaaCTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACcg                                                                                                                                                                                              >  5:1460709/1‑149 (MQ=255)

                                                                                                                                                                                                         acgggacggaggTTGGTGGCGTCAAAAGCGCCGAGGCTTTCCTGGAAGGCCGGTGGAGCCCACAGGGATGTGGGCTGAGGGCGCGTTTGACAGGGATGTTCCCTCGCGCCCGACCGGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAgatga                                                               <  4:198657/263‑1 (MQ=14)

                                                                                                                                                                                                          cTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCgcgc                                                                                                                                                                                           >  8:885345/1‑149 (MQ=255)

                                                                                                                                                                                                                  gtgtgGGGGGGGTCAAAGCTGCCGGGGGGTTACCGGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGGGGGGGCGTTTTACAGGGATGTTACCTCGCGCCCGAGCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGGGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACa                                                     <  4:143177/274‑1 (MQ=17)

                                                                                                                                                                                                                  aTGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACTCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGa                                                                                                                                                                                   <  6:1292863/149‑1 (MQ=37)

                                                                                                                                                                                                                  aTGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGa                                                                                                                                                                                   <  8:745475/149‑1 (MQ=37)

                                                                                                                                                                                                                              tCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCa                                                                                                                                                                       >  8:565149/1‑149 (MQ=35)

                                                                                                                                                                                                                                aaaaTCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCaga                                                                                                                                                                     <  5:1207510/149‑1 (MQ=35)

                                                                                                                                                                                                                                aaaaTCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCaga                                                                                                                                                                     >  6:437172/1‑149 (MQ=35)

                                                                                                                                                                                                                                            gCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGc                                                                                                                                                         >  6:834322/1‑149 (MQ=32)

                                                                                                                                                                                                                                              gTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAg                                                                                                                                                       <  8:776061/149‑1 (MQ=32)

                                                                                                                                                                                                                                                tCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAggg                                                                                                                                                     <  7:954880/149‑1 (MQ=32)

                                                                                                                                                                                                                                                 cccTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTc                                                                                                                                                                           <  7:307162/126‑1 (MQ=33)

                                                                                                                                                                                                                                                 cccTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTc                                                                                                                                                                           >  8:307162/1‑126 (MQ=33)

                                                                                                                                                                                                                                                           gccctcAGCCCACATCCATGTGGGCTGCGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCgggg                                                                                                                                                             <  5:670567/125‑1 (MQ=11)

                                                                                                                                                                                                                                                           gccctcAGCCCACATCCATGTGGGCTGCGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCgggg                                                                                                                                                             >  6:670567/6‑130 (MQ=11)

                                                                                                                                                                                                                                                           cGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGa                                                                                                                                          <  5:1179958/149‑1 (MQ=32)

                                                                                                                                                                                                                                                             gggCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGAtt                                                                                                                                        <  8:192797/149‑1 (MQ=32)

                                                                                                                                                                                                                                                              ctcAGCCCACATCCATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGggcggc                                                                                                                                               <  7:348169/139‑1 (MQ=12)

                                                                                                                                                                                                                                                              ctcAGCCCACATCCATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGggcggc                                                                                                                                               >  8:348169/3‑141 (MQ=12)

                                                                                                                                                                                                                                                               tcAGCCCACATCCATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTgg                                                                                                                                      >  6:385642/2‑149 (MQ=12)

                                                                                                                                                                                                                                                                 aGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGgcc                                                                                                                                    <  6:895251/149‑1 (MQ=32)

                                                                                                                                                                                                                                                                  gCCCACATCCATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGa                                                                                                                                                                                                      >  5:829061/1‑82 (MQ=255)

                                                                                                                                                                                                                                                                  gCCCACATCCATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGa                                                                                                                                                                                                      <  6:829061/82‑1 (MQ=255)

                                                                                                                                                                                                                                                                   cccACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGgccgc                                                                                                                                  <  6:1438439/149‑1 (MQ=32)

                                                                                                                                                                                                                                                                    ccACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGa                                                                                                                                                                                                      <  7:291126/80‑1 (MQ=34)

                                                                                                                                                                                                                                                                      acaTGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGGGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGcccc                                                                                                                               >  8:245238/1‑149 (MQ=17)

                                                                                                                                                                                                                                                                        aTGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCa                                                                                                                                                                       <  5:337937/107‑1 (MQ=32)

                                                                                                                                                                                                                                                                        aTGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCa                                                                                                                                                                       >  6:337937/1‑107 (MQ=32)

                                                                                                                                                                                                                                                                        aTGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCt                                                                                                                             >  6:1071214/1‑149 (MQ=32)

CCCACAATCAGGGGAACCAGAATGATTAACAGCCCAGAAAACATGAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTT  >  NC_000913/914814‑915349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`