Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 958,773 C→G 6.1% intergenic (+32/‑39) serC → / → aroA 3‑phosphoserine/phosphohydroxythreonine aminotransferase/5‑enolpyruvylshikimate‑3‑phosphate synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913958,7730CG 6.1% 799.3 / 31.6 226intergenic (+32/‑39)serC/aroA3‑phosphoserine/phosphohydroxythreonine aminotransferase/5‑enolpyruvylshikimate‑3‑phosphate synthetase
Reads supporting (aligned to +/- strand):  ref base C (93/119);  new base G (9/5);  total (102/124)
Fisher's exact test for biased strand distribution p-value = 1.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.41e-01

Only 100 of 235 total aligned reads displayed.

CGATTTCTACCGCAATGACGTGGCGAAAGCTAACCGTTCGCGGATGAACGTGCCGTTCCAGTTGGCGGACAGTGCGCTTGACAAATTGTTCCTTGAAGAGTCTTTTGCTGCTGGCCTTCATGCACTGAAAGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCACACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATGCATTAACAGCGTTAGGGGTAAGCTATACGC  >  NC_000913/958501‑959007
                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                          
        aCCGCAATGACGTGGCGAAAGCTAACCGTTCGCGGATGAACGTGCCGTTCCAGTTGGCGGACAGTGCGCTTGACAAATTGTTCCTTGAAGAGTCTTTTGCTGCTGGCCTTCATGCACTGAAAGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCTTGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAACTTTTGCTTCATCCCCACAGCCAGCCTGTGGGGtt                                                                                                                                                                                                                                  >  2:254792/1‑275 (MQ=255)
                    tGGCGAAAGCTAACCGTTCGCGGATGAACGTGCCGTTCCAGTTGGCGGACAGTGCGCTTGACAAATTGTTCCTTGAAGAGTCTTTTGCTGCTGGCCTTCATGCACTGAAAGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGCCAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCtttt                                                                                                                                                                                                                      >  1:243/1‑275 (MQ=255)
                                      cgcgGATGAACGTGCCGTTCCAGTTGGCGGACAGTGCGCTTGACAAATTGTTCCTTGAAGAGTCTTTTGCTGCTGGCCTTCATGCACTGAAAGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAACTTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTTTATAaagttgcgttact                                                                                                                                                                                                    >  1:5055/1‑272 (MQ=255)
                                        cgGATGAACGTGCCGTTCCAGTTGGCGGACAGTGCGCTTGACAAATTGTTCCTTGAAGAGTCTTTTGCTGCTGGCCTTCATGCACTGAAAGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATgg                                                                                                                                                                                                  <  2:158739/275‑1 (MQ=255)
                                               aCGTGCCGTTCCATGTGGCGGACAGTGCGCTTGACAAATTGTTCCCGGAAGAGTCTTTTGCTGCTGGCCTTCATGCACTGAAAGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGGTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCt                                                                                                                                                                                           <  4:254792/275‑1 (MQ=255)
                                                                gcggtacgTGCGCTTGACAAATTGTTCCTTGAAGAGTCTTTTGCTGCTGGCCTTCATGCACTGAAAGGTAACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCg                                                                                                                                                                          <  4:319261/268‑1 (MQ=255)
                                                                                          ccTTGAAGAGTCTTTTGCTGCTGGCCTTCATGCACTGAAAGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTAtt                                                                                                                                                                                                                            >  2:354394/1‑199 (MQ=255)
                                                                                              gAAGNGTCTTTTGCTGCTGGCCTTCATGCACTGAAAGGTCANCGTGNGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCtg                                                                                                                                                                                                                        <  4:354394/199‑1 (MQ=255)
                                                                                                                             tGAAAGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGACGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACagccagcc                                                                                                                                                                                                                                           >  6:1405351/1‑149 (MQ=255)
                                                                                                                             tGAAAGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACagccagcc                                                                                                                                                                                                                                           >  6:1273130/1‑149 (MQ=255)
                                                                                                                               aaaGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGttttt                                                                                                                                                                                                                               >  4:46535/1‑159 (MQ=255)
                                                                                                                                aaGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCtgt                                                                                                                                                                                                                                        <  5:992223/149‑1 (MQ=255)
                                                                                                                                 aGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCtgtg                                                                                                                                                                                                                                       <  8:802300/149‑1 (MQ=255)
                                                                                                                                 aGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCtgtg                                                                                                                                                                                                                                       <  6:376172/149‑1 (MQ=255)
                                                                                                                                  ggTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTgg                                                                                                                                                                                                                                      <  6:46629/149‑1 (MQ=255)
                                                                                                                                  ggTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCCCTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCCCTTTAttcc                                                                                                           >  4:224091/1‑270 (MQ=255)
                                                                                                                                   gTCACCGTGTGGTCGTCTGAATGCGAGCTTCAATTTATAACGCCATGCCGATGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTAACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTggc                                                                                                       <  2:224091/275‑1 (MQ=255)
                                                                                                                                        cGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGtttt                                                                                                                                                                                                                                <  8:1137411/149‑1 (MQ=255)
                                                                                                                                           gtgGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTAt                                                                                                                                                                                                                             <  7:58933/149‑1 (MQ=255)
                                                                                                                                            tgGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTAtt                                                                                                                                                                                                                            >  6:957750/1‑149 (MQ=255)
                                                                                                                                             ggTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGttttt                                                                                                                                                                                                                               >  3:71753/1‑145 (MQ=255)
                                                                                                                                              gTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTc                                                                                                                                                                                                                          <  8:556591/149‑1 (MQ=255)
                                                                                                                                               tCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCt                                                                                                                                                                                                                         <  6:1066531/149‑1 (MQ=255)
                                                                                                                                                 ggcggAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTAttt                                                                                                                                                                                                                           <  1:71753/145‑1 (MQ=255)
                                                                                                                                                 ggcggAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCtgt                                                                                                                                                                                                                       <  5:1131723/149‑1 (MQ=255)
                                                                                                                                                   gggtatgggggcTTCTATTTATAAGTCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGATTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGCGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCAGCATTAGCACACgg                                                                                        <  4:146328/264‑1 (MQ=255)
                                                                                                                                                     gAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTa                                                                                                                                                                                                                   <  5:1273130/149‑1 (MQ=255)
                                                                                                                                                      aaTGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCACACGGCaaa                                                                                    >  2:6287/1‑275 (MQ=255)
                                                                                                                                                       atgctttcttttttttATTACNNNNTTTCNTTGGAAGGCGTTAAAGCCCGGCCAGCCTGTGTGTTTTGTGTCGACCGCCGTCACGGTTAATGCCTAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCGGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCACACGGCaaaa                                                                                   <  3:243/262‑1 (MQ=255)
                                                                                                                                                       aTGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTaga                                                                                                                                                                                                                 <  5:500161/149‑1 (MQ=255)
                                                                                                                                                         gcgcgcTTCTATTTATAACGCCATGCCGCTTGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTagaga                                                                                                                                                                                                               <  5:957750/149‑1 (MQ=255)
                                                                                                                                                          cgcgcTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTagagag                                                                                                                                                                                                              <  5:801313/149‑1 (MQ=255)
                                                                                                                                                            cgcTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagtt                                                                                                                                                                                                            <  5:245821/149‑1 (MQ=255)
                                                                                                                                                              cTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagttga                                                                                                                                                                                                          <  6:77438/149‑1 (MQ=255)
                                                                                                                                                              cTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagttga                                                                                                                                                                                                          <  6:369566/149‑1 (MQ=255)
                                                                                                                                                              cTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagttga                                                                                                                                                                                                          <  6:1428220/149‑1 (MQ=255)
                                                                                                                                                               ttCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCtgtg                                                                                                                                                                                                                                       >  8:1044403/1‑119 (MQ=255)
                                                                                                                                                                   aTTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCa                                                                                                                                                                                                     >  5:395545/1‑149 (MQ=255)
                                                                                                                                                                    tttATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGGCTGGCTGTgggga                                                                                                                                                                                                                                   <  7:481310/118‑2 (MQ=255)
                                                                                                                                                                    tttATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCAt                                                                                                                                                                                                    <  7:177374/149‑1 (MQ=255)
                                                                                                                                                                    tttATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCAt                                                                                                                                                                                                    >  6:47985/1‑149 (MQ=255)
                                                                                                                                                                     ttATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATg                                                                                                                                                                                                   <  7:342512/149‑1 (MQ=255)
                                                                                                                                                                      tataACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATgg                                                                                                                                                                                                  >  5:560984/1‑149 (MQ=255)
                                                                                                                                                                          aCGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATc                                                                                                                                                                                              <  5:42414/149‑1 (MQ=255)
                                                                                                                                                                           cGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATcc                                                                                                                                                                                             <  8:522300/149‑1 (MQ=255)
                                                                                                                                                                            gCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATccc                                                                                                                                                                                            >  7:1211083/1‑149 (MQ=255)
                                                                                                                                                                              cATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTc                                                                                                                                                                                                      >  4:285597/1‑137 (MQ=255)
                                                                                                                                                                              cATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCg                                                                                                                                                                 >  4:117620/1‑174 (MQ=255)
                                                                                                                                                                               aTGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGa                                                                                                                                                                                         >  6:1219976/1‑149 (MQ=255)
                                                                                                                                                                               aTGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGa                                                                                                                                                                                         <  7:1000170/149‑1 (MQ=255)
                                                                                                                                                                                tGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGttttt                                                                                                                                                                                                                               >  2:370365/1‑110 (MQ=255)
                                                                                                                                                                                tGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGAc                                                                                                                                                                                        <  5:890385/149‑1 (MQ=255)
                                                                                                                                                                                  ccGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATgg                                                                                                                                                                                                  <  2:285597/137‑1 (MQ=255)
                                                                                                                                                                                  ccGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATgg                                                                                                                                                             <  2:117620/174‑1 (MQ=255)
                                                                                                                                                                                   cGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGtt                                                                                                                                                                                     <  8:1443504/149‑1 (MQ=255)
                                                                                                                                                                                    gCTGGAAGACGTTAAAGCGATGACAGACTTCATGGTTAAGTTCGAACGCCGTCACGGTTAATGCCGAAATATTGCTTAATCCCCACAACCAGCCTGTGGGGTTTTTCTTTCTGTCGTAGAGAGTTGAGTTCGTGGAAACCCTGACGTTa                                                                                                                                                                                    <  6:642652/149‑1 (MQ=255)
                                                                                                                                                                                     cTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTAc                                                                                                                                                                                   <  8:1211083/149‑1 (MQ=255)
                                                                                                                                                                                         aaGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGa                                                                                                                                                                <  2:12956/164‑1 (MQ=255)
                                                                                                                                                                                             cGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTagaga                                                                                                                                                                                                               <  5:1448806/113‑1 (MQ=255)
                                                                                                                                                                                                    gcgcTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCgtg                                                                                                                                                                    >  6:73941/1‑149 (MQ=255)
                                                                                                                                                                                                         gACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGAt                                                                                                                                                               <  8:1066086/149‑1 (MQ=255)
                                                                                                                                                                                                           cAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTAc                                                                                                                                                                                   <  2:269019/127‑1 (MQ=255)
                                                                                                                                                                                                              aCTTCATGGTTCAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCAc                                                                                                                                                          >  6:267431/1‑149 (MQ=255)
                                                                                                                                                                                                                ttCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTa                                                                                                                                                        >  5:1350467/1‑149 (MQ=255)
                                                                                                                                                                                                                      gTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGccc                                                                                                                                             <  4:125055/154‑1 (MQ=255)
                                                                                                                                                                                                                        tGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTg                                                                                                                                                >  5:374489/1‑149 (MQ=255)
                                                                                                                                                                                                                               gAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGtt                                                                                                                                         >  7:200234/1‑149 (MQ=255)
                                                                                                                                                                                                                                   gCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCaa                                                                                                                                     <  5:1035799/149‑1 (MQ=255)
                                                                                                                                                                                                                                   gCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCaa                                                                                                                                     <  6:560984/149‑1 (MQ=255)
                                                                                                                                                                                                                                     cGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAaga                                                                                                                                   <  7:1064966/149‑1 (MQ=255)
                                                                                                                                                                                                                                       tCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGc                                                                                                                                 <  8:1017696/149‑1 (MQ=255)
                                                                                                                                                                                                                                          cGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGtt                                                                                                                              <  7:738638/149‑1 (MQ=255)
                                                                                                                                                                                                                                                    cGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACcgcg                                                                                                                    >  6:673449/1‑149 (MQ=255)
                                                                                                                                                                                                                                                     gAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCgcgc                                                                                                                   >  6:1478068/1‑149 (MQ=255)
                                                                                                                                                                                                                                                      aaaTTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCt                                                                                                                  <  8:1310057/149‑1 (MQ=255)
                                                                                                                                                                                                                                                      aaaTTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCt                                                                                                                  <  8:1160040/149‑1 (MQ=255)
                                                                                                                                                                                                                                                         ttttGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTa                                                                                                               >  7:221851/1‑149 (MQ=255)
                                                                                                                                                                                                                                                           ttGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTAtt                                                                                                             >  8:47652/1‑149 (MQ=255)
                                                                                                                                                                                                                                                              cTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCt                                                                                                          >  5:492816/1‑149 (MQ=255)
                                                                                                                                                                                                                                                              cTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCt                                                                                                          <  8:274063/149‑1 (MQ=255)
                                                                                                                                                                                                                                                               ttAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATgg                                                                                                                                                             >  8:321171/1‑97 (MQ=255)
                                                                                                                                                                                                                                                               ttAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTg                                                                                                         >  7:1310423/1‑149 (MQ=255)
                                                                                                                                                                                                                                                               ttAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTg                                                                                                         <  7:1372943/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                 aaTCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGcc                                                                                                                                              <  5:218909/110‑1 (MQ=255)
                                                                                                                                                                                                                                                                   tCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTggcgg                                                                                                     >  8:36767/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                   accccACAGGCTGGCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTggcgg                                                                                                     >  7:457802/2‑149 (MQ=255)
                                                                                                                                                                                                                                                                    ccccACAGGCTGGCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTggcggc                                                                                                    >  7:921526/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                     cccACAGGCTGGCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCa                                                                                                   >  8:1358008/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                     cccACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCa                                                                                                   >  7:1427785/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                      ccACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCt                                                                                                                                                 >  8:881873/1‑102 (MQ=255)
                                                                                                                                                                                                                                                                      ccACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCt                                                                                                                                                 <  7:881873/102‑1 (MQ=255)
                                                                                                                                                                                                                                                                      ccACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTgg                                                                                                        >  7:1154865/1‑143 (MQ=255)
                                                                                                                                                                                                                                                                      ccACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCAt                                                                                                  <  6:1155386/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                      ccACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCAt                                                                                                  >  6:897620/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                      ccACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCAt                                                                                                  >  7:769391/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                        acaGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTAtt                                                                                                             <  5:707306/136‑1 (MQ=255)
                                                                                                                                                                                                                                                                        acaGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTa                                                                                                <  5:673449/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                          agccagccTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGc                                                                                              <  5:73941/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                           gccagccTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGca                                                                                             <  5:267431/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                               gccTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCACACg                                                                                         <  6:80588/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                          
CGATTTCTACCGCAATGACGTGGCGAAAGCTAACCGTTCGCGGATGAACGTGCCGTTCCAGTTGGCGGACAGTGCGCTTGACAAATTGTTCCTTGAAGAGTCTTTTGCTGCTGGCCTTCATGCACTGAAAGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCACACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATGCATTAACAGCGTTAGGGGTAAGCTATACGC  >  NC_000913/958501‑959007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: