BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,015,912	T→A	11.7%	intergenic (+30/+40)	rmf → / ← fabA	ribosome modulation factor/beta‑hydroxydecanoyl thioester dehydrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,015,912	0	T	A	11.7%	774.4 / 75.9	251	intergenic (+30/+40)	rmf/fabA	ribosome modulation factor/beta‑hydroxydecanoyl thioester dehydrase
Reads supporting (aligned to +/- strand): ref base T (96/125); new base A (17/13); total (113/138)
Fisher's exact test for biased strand distribution p-value = 1.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.44e-02

Only 100 of 253 total aligned reads displayed.

GTGATTGCACATTTAGTAATCACTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCT‑GGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGACCAGTGAATTTCACTTCGCCAACGCCCAGCGCG  >  NC_000913/1015650‑1016156

gtgATTGCACATTTAGTAATCACTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGACCGCCT‑GGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGA‑AATGTGTCCCTATCAGACGCTGAATCCAAGGTCACAATGGCTGGGAGGCTGGCGAAAAGCCATGGAGGCCAGGGTAGTATTGGCCTGAGTCTGTCTCTTTAAAAACAACCCTCCGCATTTCGGCGGtt                                                                                                                                                                                                                                            >  4:67103/1‑273 (MQ=255)

   aTTGCACATTTAGTAATCACTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCT‑GGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCGGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGAGGAGGtt                                                                                                                                                                                                                                            >  1:66553/1‑270 (MQ=255)

      gCACATTTAGTAATAACTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCTGGGAACTGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTTGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCtt                                                                                                                                                                                                                                     <  2:142175/275‑1 (MQ=255)

            ttAGTAATCACTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCT‑GGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATAc                                                                                                                                                                                                                              <  1:10086/275‑1 (MQ=255)

                                                                       ggaCAAAAACGGAATGGCCT‑GGAAAGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGc                                                                                                                                                                   <  4:311829/274‑1 (MQ=255)

                                                                          cAAAAACGAGATAGCCT‑GAAACGGGCAGAGTAACGAGGTTATCAGGCCGGCAACGCCGGAAGCTCAAAAGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATGGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTgg                                                                                                                                                                <  4:289437/275‑1 (MQ=255)

                                                                           aaaaaCGAGATCGCCT‑GGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTgg                                                                                                                                                                <  2:185224/274‑1 (MQ=255)

                                                                                                                        gCCGGCATCGCCGGACGCTCAAAAGA‑AATGTGTCCCTATCAGAAGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCa                                                                                                                   <  2:67103/275‑1 (MQ=255)

                                                                                                                        gCCGGAATCGGCGGACGC‑CAAAAGAGAATGTGTCCCATTCGAACGCTGAATCAAAGGGCAAAATGGCTGGGATGCTGGCGAGAAGCCATGGTGGACAGGGTAGTAATGGCGTGATTCTGTCTTTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTTTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCa                                                                                                                   <  4:211473/275‑1 (MQ=255)

                                                                                                                         ccGGCATCGCCGGACGCTCAAAAGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGa                                                                                                                                                                                                                                                <  6:100949/149‑1 (MQ=255)

                                                                                                                         ccGGCATCGCCGGACGCTCAAAAGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGa                                                                                                                                                                                                                                                <  5:292961/149‑1 (MQ=255)

                                                                                                                         ccGGCATCGCCGGACGCTCAAAAGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGa                                                                                                                                                                                                                                                <  5:1153838/149‑1 (MQ=255)

                                                                                                                         ccGGCATCGCCGGACGCTCAAAAGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGa                                                                                                                                                                                                                                                <  5:1080729/149‑1 (MQ=255)

                                                                                                                         ccGGCATCGCCGGACGCTCAAAAGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGa                                                                                                                                                                                                                                                <  8:65230/149‑1 (MQ=255)

                                                                                                                          cGGCATCGCCGGACGCTCAAAAGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCAATGCGGAg                                                                                                                                                                                                                                               <  7:997886/149‑1 (MQ=255)

                                                                                                                           ggCATCGCCGGACGCTCAAAAGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCCACCCCCAgg                                                                                                                 >  2:31355/1‑274 (MQ=255)

                                                                                                                            gCATCGCCGGACGCTCAAAAGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGt                                                                                                                                                                                                                                             <  5:387480/149‑1 (MQ=255)

                                                                                                                            gCATCGCCGGACGCTCAAAAGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCAATGCGGAg                                                                                                                                                                                                                                               >  5:920316/1‑147 (MQ=255)

                                                                                                                            gCATCGCCGGACGCTCAAAAGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCAATGCGGAGGt                                                                                                                                                                                                                                             <  8:369444/149‑1 (MQ=255)

                                                                                                                            gCATCGCCGGACGCTCAAAAGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCAATGCGGAGGt                                                                                                                                                                                                                                             <  6:1140282/149‑1 (MQ=255)

                                                                                                                             cATCGCCGGACGCTCAAAAGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGtt                                                                                                                                                                                                                                            <  7:1451449/149‑1 (MQ=255)

                                                                                                                               tCGCCGGACGCTCAAAAGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTc                                                                                                                                                                                                                                          <  5:525311/149‑1 (MQ=255)

                                                                                                                                     gACGCTCAAAAGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCttt                                                                                                                                                                                                                                    <  7:804270/149‑1 (MQ=255)

                                                                                                                                     gACGCTCAAAAGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCttt                                                                                                                                                                                                                                    <  5:709307/149‑1 (MQ=255)

                                                                                                                                       caatCAAAAGA‑AATGTGTCCCTATCAGACGCTGAATGAAAGGTCACAATGGCTGGGAGGCTGGCGCGAAGCCATGGNGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAga                                                                                                     <  3:66553/271‑1 (MQ=255)

                                                                                                                                          tCAAAAGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATa                                                                                                                                                                                                                               >  6:919431/1‑149 (MQ=255)

                                                                                                                                            aaaaGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGa                                                                                                                                                                                                                                 <  5:622297/145‑1 (MQ=255)

                                                                                                                                            aaaaGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGa                                                                                                                                                                                                                                 >  6:622297/1‑145 (MQ=255)

                                                                                                                                            aaaaGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATAct                                                                                                                                                                                                                             >  8:91062/1‑149 (MQ=255)

                                                                                                                                            aaaaGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATAct                                                                                                                                                                                                                             >  8:294937/1‑149 (MQ=255)

                                                                                                                                              aaGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATActct                                                                                                                                                                                                                           >  8:825109/1‑149 (MQ=255)

                                                                                                                                              aaGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATActct                                                                                                                                                                                                                           >  7:1110046/1‑149 (MQ=255)

                                                                                                                                                   aaTGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGTATTGCGGAGGTTTCGCCTTTTGATACtctgtct                                                                                                                                                                                                                       <  7:120827/149‑1 (MQ=255)

                                                                                                                                                     tgtgTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGa                                                                                                                                                                                                                     <  6:1292311/149‑1 (MQ=255)

                                                                                                                                                     tgtgTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGa                                                                                                                                                                                                                     <  5:1159486/149‑1 (MQ=255)

                                                                                                                                                       tgtCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGttt                                                                                                                                                                                                                                           >  4:172610/1‑125 (MQ=255)

                                                                                                                                                       tgtCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGAtt                                                                                                                                                                                                                   <  7:1149717/149‑1 (MQ=255)

                                                                                                                                                       tgtCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGAtt                                                                                                                                                                                                                   <  8:157272/149‑1 (MQ=255)

                                                                                                                                                         tCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATtat                                                                                                                                                                                                                 >  7:1025123/1‑149 (MQ=255)

                                                                                                                                                          cccTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATtata                                                                                                                                                                                                                >  7:812601/1‑149 (MQ=255)

                                                                                                                                                           ccTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGcc                                                                                                                                                                                                                                       <  2:172610/125‑1 (MQ=255)

                                                                                                                                                           ccTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATaa                                                                                                                                                                                                               >  8:1251634/1‑149 (MQ=255)

                                                                                                                                                            cTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGGCAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAAt                                                                                                                                                                                                              <  8:715861/149‑1 (MQ=255)

                                                                                                                                                            cTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAAt                                                                                                                                                                                                              >  6:478680/1‑149 (MQ=255)

                                                                                                                                                            cTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAAt                                                                                                                                                                                                              >  6:186969/1‑149 (MQ=255)

                                                                                                                                                             tATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACtctgtct                                                                                                                                                                                                                       <  7:927804/139‑1 (MQ=255)

                                                                                                                                                             tATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACtctgtct                                                                                                                                                                                                                       >  8:927804/1‑139 (MQ=255)

                                                                                                                                                             tATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCAATGCGGAg                                                                                                                                                                                                                                               >  6:894657/1‑115 (MQ=255)

                                                                                                                                                             tATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATAGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATc                                                                                                                                                                                                             <  5:1281277/149‑1 (MQ=255)

                                                                                                                                                                 aGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGaa                                                                                                                                                                                                         <  7:294120/149‑1 (MQ=255)

                                                                                                                                                                 aGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGaa                                                                                                                                                                                                         <  7:1387987/149‑1 (MQ=255)

                                                                                                                                                                     gCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCa                                                                                                                                                                                                     >  6:426419/1‑149 (MQ=255)

                                                                                                                                                                       tGAATCAAAGGTCACAATGGCTGGGAGGCTGNCGAGNAGCCATGGCGGACAGGGTAGTAANGGCCTGATTCTGTCTCTTTAAAAAGAAACCGCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTgg                                                                                                                                                                <  4:347064/184‑1 (MQ=255)

                                                                                                                                                                       tGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGTGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGTCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGa                                                                                                                                                                                                   <  5:1211671/149‑1 (MQ=255)

                                                                                                                                                                       tGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGa                                                                                                                                                                                                   <  8:508835/149‑1 (MQ=255)

                                                                                                                                                                       tGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGa                                                                                                                                                                                                   <  5:1439358/149‑1 (MQ=255)

                                                                                                                                                                        gAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGAc                                                                                                                                                                                                  <  8:586216/149‑1 (MQ=255)

                                                                                                                                                                         aaTCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGcc                                                                                                                                                                                                                                       >  4:111089/1‑111 (MQ=255)

                                                                                                                                                                           tCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATtata                                                                                                                                                                                                                >  7:318711/1‑132 (MQ=255)

                                                                                                                                                                           tCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGc                                                                                                                                                                                                      <  5:919400/142‑1 (MQ=255)

                                                                                                                                                                             aaaGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATc                                                                                                                                                                                             >  8:808817/1‑149 (MQ=255)

                                                                                                                                                                             aaaGGTCACAATGGCTGGGAGGCTAGCGAGAAGCCATGTCGGACAGGGTAGTAATGGTCTTGTTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCtttt                                                                                                                                                                                                                                   <  2:111089/111‑1 (MQ=255)

                                                                                                                                                                                 gTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTgg                                                                                                                                                                                         >  7:183362/1‑149 (MQ=255)

                                                                                                                                                                                      aaTGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAg                                                                                                                                                                                    >  7:734956/1‑149 (MQ=255)

                                                                                                                                                                                           cTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTg                                                                                                                                                                                          >  8:1132412/1‑138 (MQ=255)

                                                                                                                                                                                                aGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCg                                                                                                                                                                    >  2:86348/1‑155 (MQ=255)

                                                                                                                                                                                                aGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGc                                            >  4:244683/1‑275 (MQ=255)

                                                                                                                                                                                                aGGATGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCgg                                             <  2:335275/274‑1 (MQ=255)

                                                                                                                                                                                                 ggCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCa                                                                                                                                                                         >  5:1049886/1‑149 (MQ=255)

                                                                                                                                                                                                 ggCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTATGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCa                                                                                                                                                                         <  6:1266007/149‑1 (MQ=255)

                                                                                                                                                                                                    tGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGt                                                                                                                                                                      >  6:1272421/1‑149 (MQ=255)

                                                                                                                                                                                                      gCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCg                                                                                                                                                                    >  7:254702/1‑149 (MQ=255)

                                                                                                                                                                                                       cGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGc                                                                                                                                                                   >  5:792890/1‑149 (MQ=255)

                                                                                                                                                                                                            aGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTAGCTGGCg                                                                                                                                                              <  6:792890/149‑1 (MQ=255)

                                                                                                                                                                                                              ccATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGt                                                                                                                                                            >  6:57926/1‑149 (MQ=255)

                                                                                                                                                                                                                   gCGTACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGa                                                                                                                                                       <  5:1223099/149‑1 (MQ=255)

                                                                                                                                                                                                                    cGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGAt                                                                                                                                                      <  7:12924/149‑1 (MQ=255)

                                                                                                                                                                                                                      gACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCa                                                                                                                                                    <  7:1251634/149‑1 (MQ=255)

                                                                                                                                                                                                                            gtagtaATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTAGCTGGCGGTATAGATCAgacgac                                                                                                                                              >  8:777743/1‑149 (MQ=255)

                                                                                                                                                                                                                                taATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATc                                                                                                                                          >  7:897179/1‑149 (MQ=255)

                                                                                                                                                                                                                                  aTGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTG‑TGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAAc                                                                                                                                       >  6:1039661/1‑149 (MQ=255)

                                                                                                                                                                                                                                     gCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAgacg                                                                                                                                                >  2:272323/1‑138 (MQ=255)

                                                                                                                                                                                                                                      ccTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAgacg                                                                                                                                                >  4:179011/1‑137 (MQ=255)

                                                                                                                                                                                                                                       cTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGc                                                                                                                                                               >  2:221286/1‑121 (MQ=255)

                                                                                                                                                                                                                                         gATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCa                                                                                                                                            <  4:272323/138‑1 (MQ=255)

                                                                                                                                                                                                                                            tCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTc                                                                                                                              >  8:1225789/1‑149 (MQ=255)

                                                                                                                                                                                                                                              tGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGAt                                                                                                                                                      <  6:946391/123‑1 (MQ=255)

                                                                                                                                                                                                                                                   ctTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATcc                                                                                                                       <  8:254702/149‑1 (MQ=255)

                                                                                                                                                                                                                                                   ctTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATcc                                                                                                                       <  8:187896/149‑1 (MQ=255)

                                                                                                                                                                                                                                                    tttAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTc                                                                                                                                                                     >  8:1336357/1‑102 (MQ=255)

                                                                                                                                                                                                                                                    tttAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCg                                                                                                                      >  7:9843/1‑149 (MQ=255)

                                                                                                                                                                                                                                                         aaaGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAgg                                                                                                                 >  7:554844/1‑149 (MQ=255)

                                                                                                                                                                                                                                                           aGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCa                                                                                                                                                                         >  6:1157526/1‑91 (MQ=255)

                                                                                                                                                                                                                                                              aaCCTCCGCAATGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCAt                                                                                                            >  6:729249/1‑149 (MQ=255)

                                                                                                                                                                                                                                                               aCCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATa                                                                                                           >  7:1369195/1‑149 (MQ=255)

                                                                                                                                                                                                                                                               aCCTCCGCAATGCGGTGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGTTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCTAGGCCCATa                                                                                                           >  8:730385/1‑149 (MQ=255)

                                                                                                                                                                                                                                                               aCCTCCGCAATGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAgg                                                                                                                 <  7:511499/143‑1 (MQ=255)

                                                                                                                                                                                                                                                                  tCCGCATTGCGGAGGTTTCGCCTTTTGATACtctgtc                                                                                                                                                                                                                        <  7:710113/37‑1 (MQ=255)

                                                                                                                                                                                                                                                                  tCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAAc                                                                                                                                       <  8:531975/118‑1 (MQ=255)

                                                                                                                                                                                                                                                                   ccGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCa                                                                                                       >  5:228907/1‑149 (MQ=255)

GTGATTGCACATTTAGTAATCACTGTTTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGCATGAAGAGACAAAAACGAGATCGCCT‑GGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGA‑AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGACCAGTGAATTTCACTTCGCCAACGCCCAGCGCG  >  NC_000913/1015650‑1016156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`