Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,233,157 T→A 8.4% intergenic (‑127/+19) dsbB ← / ← nhaB oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I/sodium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,233,1570TA 8.4% 717.3 / 37.1 209intergenic (‑127/+19)dsbB/nhaBoxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I/sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base T (85/105);  new base A (10/9);  total (95/114)
Fisher's exact test for biased strand distribution p-value = 6.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 219 total aligned reads displayed.

AGCACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGC  >  NC_000913/1232903‑1233412
                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                               
agCACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATNATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGAANCAAACATAGGAAGCAATTGGACAATTATCGGGCGAAAAGGCAAACNGNGCATTTAAATGCCCGGGAAggtgttta                                                                                                                                                                                                                                              >  3:39274/1‑272 (MQ=255)
                                    cacaGTGCCGTCATTTCAAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGACCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCAtt                                                                                                                                                                                                         <  1:39274/275‑1 (MQ=255)
                                                aGTTCCATGGACAGAGAAGTAAAAGCCAACAACAGCACCGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAgggg                                                                                                                                                                                             <  4:19819/275‑1 (MQ=255)
                                                                                                       aaCATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAATCCGACGAGTTTCAGGACGAGGGGGTAAg                                                                                                                                       >  2:224625/1‑274 (MQ=255)
                                                                                                          aTTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAgg                                                                                                                                                    >  4:230758/1‑258 (MQ=255)
                                                                                                           ttGGTTCAAAAATTGCAACATAATCATTTCCCTGCAATATGCATAGAGAGGTCAGTTTAAACCAATTCATGCGCTGCGCCACCAGGAGGCAGCAAAAATCGGACGCAATTGGACAATTATCGAACGAAAAGGCAAACCGGGCATTAATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGTAGAGCAAGCCGACGAGTGGCAGGACGAGGGTGTAAGGCAgg                                                                                                                                  <  4:127271/275‑1 (MQ=255)
                                                                                                            tGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTatat                                                                                                                                                                                                                                                               <  5:374649/149‑1 (MQ=255)
                                                                                                                tCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGccc                                                                                                                                                                                                                                                           <  5:123318/149‑1 (MQ=255)
                                                                                                                tCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGccc                                                                                                                                                                                                                                                           >  8:372421/1‑149 (MQ=255)
                                                                                                                  aaaaaTCGCAACATAATCATTTCCCTGCAATATGCATGGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATATAAATGCCCgg                                                                                                                                                                                                                                                         <  6:1213347/149‑1 (MQ=255)
                                                                                                                  aaaaaTCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCgg                                                                                                                                                                                                                                                         >  5:369927/1‑149 (MQ=255)
                                                                                                                   aaaaTCGCAACCTAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATATAAATGCCCGGt                                                                                                                                                                                                                                                        <  5:1402422/149‑1 (MQ=255)
                                                                                                                    aaaTCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTa                                                                                                                                                                                                                                                       >  5:654626/1‑149 (MQ=255)
                                                                                                                       tCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAgt                                                                                                                                                                                                                                                    <  7:916557/149‑1 (MQ=255)
                                                                                                                          cAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAgttgt                                                                                                                                                                                                                                                 <  5:882220/149‑1 (MQ=255)
                                                                                                                           aaCATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGGAAAGGCAAACCGGGCATTTATATGCCCGGTAAgttgtt                                                                                                                                                                                                                                                >  6:8189/1‑149 (MQ=255)
                                                                                                                           aaCATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGt                                                                                                                                                                                                                                                        >  7:902581/1‑141 (MQ=255)
                                                                                                                           aaCATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGt                                                                                                                                                                                                                                                        <  8:902581/141‑1 (MQ=255)
                                                                                                                           aaCATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAgttgtt                                                                                                                                                                                                                                                <  7:599414/149‑1 (MQ=255)
                                                                                                                             cATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTAt                                                                                                                                                                                                                                              >  5:381673/1‑149 (MQ=255)
                                                                                                                              aTAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATc                                                                                                                                                                                                                                             <  6:767338/149‑1 (MQ=255)
                                                                                                                                   cATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGc                                                                                                                                                                                                                                        <  5:440508/149‑1 (MQ=255)
                                                                                                                                   cATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGc                                                                                                                                                                                                                                        <  8:68151/149‑1 (MQ=255)
                                                                                                                                     tttCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGt                                                                                                                                                                                                                                      <  5:493820/149‑1 (MQ=255)
                                                                                                                                      ttCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGtt                                                                                                                                                                                                                                     <  7:182467/149‑1 (MQ=255)
                                                                                                                                      ttCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGtt                                                                                                                                                                                                                                     <  5:294482/149‑1 (MQ=255)
                                                                                                                                      ttCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGtt                                                                                                                                                                                                                                     <  5:1030718/149‑1 (MQ=255)
                                                                                                                                          cTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCACGCGTACACTCGACTCAGAGCAAGCCGCCGAGTTGCAGGTCGAGGGTTTACGGCAGGGCCATCCACACCATGGCGCACAAAGAGAgg                                                                                                    >  4:280949/1‑274 (MQ=255)
                                                                                                                                           tGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTAt                                                                                                                                                                                                                                <  7:495228/149‑1 (MQ=255)
                                                                                                                                             cAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTAACTATcc                                                                                                                                                                                                                              >  6:505897/1‑149 (MQ=255)
                                                                                                                                                 atGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGcc                                                                                                                                                                                                                          <  7:1022587/149‑1 (MQ=255)
                                                                                                                                                   gCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCa                                                                                                                                                                                                                        >  6:283142/1‑149 (MQ=255)
                                                                                                                                                   gCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCa                                                                                                                                                                                                                        <  5:704389/149‑1 (MQ=255)
                                                                                                                                                    cATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCAt                                                                                                                                                                                                                       >  8:435541/1‑149 (MQ=255)
                                                                                                                                                     aTAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATATAAATGCCCGGttt                                                                                                                                                                                                                                                      <  8:927613/117‑3 (MQ=255)
                                                                                                                                                         agTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCa                                                                                                                                                                                                                  <  7:378889/149‑1 (MQ=255)
                                                                                                                                                         agTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCa                                                                                                                                                                                                                  <  6:1030535/149‑1 (MQ=255)
                                                                                                                                                             cgcAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATaaa                                                                                                                                                                                                              <  5:1471768/149‑1 (MQ=255)
                                                                                                                                                                  tttAAACCAATTCATTCGCTGCGCCTCCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTTTCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCAtt                                                                                                                                                                                                         >  5:1431043/1‑149 (MQ=255)
                                                                                                                                                                   ttAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTc                                                                                                                                                                                                        <  5:975516/149‑1 (MQ=255)
                                                                                                                                                                    tAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCACTTGCATAAGCCATTCg                                                                                                                                                                                                       <  6:446112/149‑1 (MQ=255)
                                                                                                                                                                       aCCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGtt                                                                                                                                                                                                    >  7:1407244/1‑149 (MQ=255)
                                                                                                                                                                        ccAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTa                                                                                                                                                                                                   >  8:171379/1‑149 (MQ=255)
                                                                                                                                                                         cAATTCATTCGCTGCGCCACCAGGAGGCAGGAAAAATAGGACGCATTTGGACAATTATTTGTCGAAAAGGCAAACCGGGCATTTATATGCCCGGGAAGTTGTTATCAAAGCGTTGCTATCCAGCCAATTTGCATAAACTATTCGGTTAc                                                                                                                                                                                                  >  6:931160/1‑149 (MQ=255)
                                                                                                                                                                         cAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTAc                                                                                                                                                                                                  >  7:167471/1‑149 (MQ=255)
                                                                                                                                                                         cAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTAc                                                                                                                                                                                                  >  5:1010359/1‑149 (MQ=255)
                                                                                                                                                                         cAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTAc                                                                                                                                                                                                  >  8:50615/1‑149 (MQ=255)
                                                                                                                                                                            ttcattcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACCCCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTcagaag                                                                    >  2:268914/1‑272 (MQ=255)
                                                                                                                                                                            ttcattcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGGGTGCAGAGGTcagcag                                                                    >  2:287898/1‑272 (MQ=255)
                                                                                                                                                                            ttcattcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTcagcag                                                                    >  2:302821/1‑272 (MQ=255)
                                                                                                                                                                             tcattcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAggg                                                                                                                                                                                              <  5:323274/149‑1 (MQ=255)
                                                                                                                                                                               attcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGc                                                                                                                                                                                            <  5:499446/149‑1 (MQ=255)
                                                                                                                                                                               attcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGc                                                                                                                                                                                            >  6:526674/1‑149 (MQ=255)
                                                                                                                                                                                ttcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATcc                                                                                                                                                                                                                              >  6:1476452/1‑114 (MQ=255)
                                                                                                                                                                                ttcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCa                                                                                                                                                                                           >  7:746583/1‑149 (MQ=255)
                                                                                                                                                                                 tcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGcaggaaca                                                               <  2:58352/272‑1 (MQ=255)
                                                                                                                                                                                  cGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAg                                                                                                                                                                                         >  8:1242686/1‑149 (MQ=255)
                                                                                                                                                                                   gCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGc                                                                                                                                                                                        <  6:534888/149‑1 (MQ=255)
                                                                                                                                                                                    gtggggcaccaGGAGGAAGAAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACg                                                         <  4:302821/269‑1 (MQ=255)
                                                                                                                                                                                       cgcCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAg                                                      >  2:171775/1‑275 (MQ=255)
                                                                                                                                                                                        gcCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACGATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTaaa                                                                                                                                                                                   <  5:836561/149‑1 (MQ=255)
                                                                                                                                                                                         ccaccaGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAAc                                                                                                                                                                                  <  7:1337713/149‑1 (MQ=255)
                                                                                                                                                                                           accaGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTc                                                                                                                                                                                >  8:664019/1‑149 (MQ=255)
                                                                                                                                                                                           accaGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTc                                                                                                                                                                                >  8:460761/1‑149 (MQ=255)
                                                                                                                                                                                             caGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTc                                                                                                                                                                                                        <  5:448977/123‑1 (MQ=255)
                                                                                                                                                                                             caGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGa                                                                                                                                                                              <  7:478339/149‑1 (MQ=255)
                                                                                                                                                                                              aggaggCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGAc                                                                                                                                                                             >  6:1360283/1‑149 (MQ=255)
                                                                                                                                                                                                gaggCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGc                                                                                                                                                                           >  8:1024839/1‑149 (MQ=255)
                                                                                                                                                                                                       caAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCaa                                                                                                                                                                    <  7:970302/149‑1 (MQ=255)
                                                                                                                                                                                                        aaaaaTAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCAt                                                                                                                                                                                                                       >  7:650309/1‑97 (MQ=255)
                                                                                                                                                                                                        aaaaaTAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAg                                                                                                                                                                   >  8:1419602/1‑149 (MQ=255)
                                                                                                                                                                                                               ggACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCcgacga                                                                                                                                                            <  5:193864/149‑1 (MQ=255)
                                                                                                                                                                                                                     aaTTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCa                                                                                                                                                      >  5:536430/1‑149 (MQ=255)
                                                                                                                                                                                                                        tGGAAAAGTATCGGGACAAAAGGAAAACCGGGAATTTATATGCCCGGTAAGTTGTTATCAAAGCGTGGCTATCCAGCCCATGTGCATAAACCATTCGGTTACATGGGCAAGCGTAAACTCGACGCAGGGCAAGCCGACGAGTGTCAGGACGAGGGTGGAAGGCAGGGCCAGCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGAt                     <  4:6204/275‑1 (MQ=255)
                                                                                                                                                                                                                         ggACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAc                                                                                                                                                  <  7:317881/149‑1 (MQ=255)
                                                                                                                                                                                                                           aCAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGa                                                                                                                                                <  5:1384285/149‑1 (MQ=255)
                                                                                                                                                                                                                           aCAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGa                                                                                                                                                <  6:892812/149‑1 (MQ=255)
                                                                                                                                                                                                                            cAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAg                                                                                                                                               <  7:1419602/149‑1 (MQ=255)
                                                                                                                                                                                                                             aaTTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCCAAGCGTTGCTATCCAGCCCATTTGCATAAACCCTTCGGTTACAGGTGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAgg                                                                                                                                              <  8:1389093/149‑1 (MQ=255)
                                                                                                                                                                                                                             aaTTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAgg                                                                                                                                              <  8:547779/149‑1 (MQ=255)
                                                                                                                                                                                                                             aaTTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAgg                                                                                                                                              <  7:269727/149‑1 (MQ=255)
                                                                                                                                                                                                                             aaTTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAgg                                                                                                                                              >  5:763424/1‑149 (MQ=255)
                                                                                                                                                                                                                               ttATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTACACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGCGGCGAATCCATGGCGCGAGTGCAGAGGCCAGCAGGACCAGGAACGCAGCATAACCGTTCCGCGTAGCGACGGAGGGCAGATTGGtcc               >  4:41768/1‑272 (MQ=255)
                                                                                                                                                                                                                               ttATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGgt                                                                                                                                            <  6:373637/149‑1 (MQ=255)
                                                                                                                                                                                                                               ttATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCAGTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTAcc              <  4:294810/275‑1 (MQ=255)
                                                                                                                                                                                                                                   cGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCag                                                                                                                                                                         <  6:596874/116‑1 (MQ=255)
                                                                                                                                                                                                                                   cGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTaa                                                                                                                                        <  7:869894/149‑1 (MQ=255)
                                                                                                                                                                                                                                   cGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATg                                                                                                                   <  2:71064/170‑1 (MQ=255)
                                                                                                                                                                                                                                    gggCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTCCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAg                                                                                                                                       <  7:993689/149‑1 (MQ=255)
                                                                                                                                                                                                                                        gAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAg                                                                                                                                   >  5:1169732/1‑149 (MQ=255)
                                                                                                                                                                                                                                         aaaaGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAgg                                                                                                                                  >  5:637290/1‑149 (MQ=255)
                                                                                                                                                                                                                                            aGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGcc                                                                                                                               <  7:435541/149‑1 (MQ=255)
                                                                                                                                                                                                                                              acttaCCGGGCATATAAATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAg                                                                                                                                               <  7:574726/127‑1 (MQ=255)
                                                                                                                                                                                                                                              acttaCCGGGCATATAAATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAg                                                                                                                                               >  8:574726/5‑131 (MQ=255)
                                                                                                                                                                                                                                               cttaCCGGGCATATAAATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATc                                                                                                                            <  6:399779/146‑1 (MQ=255)
                                                                                                                                                                                                                                               cAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATATACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATc                                                                                                                            <  8:825025/149‑1 (MQ=255)
                                                                                                                                                                                                                                               cAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATc                                                                                                                            <  6:1010359/149‑1 (MQ=255)
                                                                                                                                                                                                                                               cAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATc                                                                                                                            <  6:96778/149‑1 (MQ=255)
                                                                                                                                                                                                                                                ttaCCGGGCATATAAATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATcc                                                                                                                           >  6:332726/3‑149 (MQ=255)
                                                                                                                                                                                                                                                           ttATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATATACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCgg                                                                                                                <  5:1200073/149‑1 (MQ=255)
                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                               
AGCACGCAAGGTTTCAGTAACATCACATGCTGGAACCACAGCGCCGTCAGTTCCAGTGCCAGAGCAGTAAACGCCATCAACAGCCACGCGCCCCGGCCTTGTGAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGC  >  NC_000913/1232903‑1233412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: