Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 897,973 Δ1 bp 5.4% intergenic (+44/‑16) potI → / → ybjO putrescine ABC transporter permease/DUF2593 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913897,9730T. 5.4% 590.0 / 24.1 166intergenic (+44/‑16)potI/ybjOputrescine ABC transporter permease/DUF2593 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (82/74);  new base . (6/3);  total (88/78)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

Only 100 of 166 total aligned reads displayed.

GATCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCTGATGATTTTC  >  NC_000913/897827‑898120
                                                                                                                                                   |                                                                                                                                                   
gATCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTcg                                                                                                                                                   >  1:1334268/1‑149 (MQ=255)
 aTCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTcgc                                                                                                                                                  >  3:1071936/1‑149 (MQ=255)
    cTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCGGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGc                                                                                                                                               <  1:72991/149‑1 (MQ=255)
    cTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGc                                                                                                                                               <  2:1237849/149‑1 (MQ=255)
          gcgGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGtt                                                                                                                                         <  3:1276020/149‑1 (MQ=255)
          gcgGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGtt                                                                                                                                         >  1:207497/1‑149 (MQ=255)
            ggTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGtttt                                                                                                                                       >  4:643264/1‑149 (MQ=255)
            ggTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGtttt                                                                                                                                       <  4:411596/149‑1 (MQ=255)
            ggTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGtttt                                                                                                                                       <  3:643264/149‑1 (MQ=255)
              tCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCa                                                                                                                                     >  2:428031/1‑149 (MQ=255)
              tCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCa                                                                                                                                     <  2:1457440/149‑1 (MQ=255)
               cGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCAt                                                                                                                                    <  2:1401556/149‑1 (MQ=255)
               cGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCAt                                                                                                                                    <  3:1040994/149‑1 (MQ=255)
               cGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCAt                                                                                                                                    <  1:784860/149‑1 (MQ=255)
                     tGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGa                                                                                                                              <  4:1162643/149‑1 (MQ=255)
                          gATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGaaa                                                                                                                         <  3:1369599/149‑1 (MQ=255)
                           aTTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAAc                                                                                                                        <  1:565631/149‑1 (MQ=255)
                           aTTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAAc                                                                                                                        >  1:1070778/1‑149 (MQ=255)
                               aTCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAAc                                                                                                                        <  2:777953/145‑1 (MQ=255)
                               aTCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAAc                                                                                                                        >  1:777953/1‑145 (MQ=255)
                               aTCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTAACTAATGCCGCGCATGTCGCGGCATTGTTTTCTTGGAAGACGAAACGTTg                                                                                                                    >  4:1231356/1‑149 (MQ=255)
                                tCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTgg                                                                                                                   >  4:12819/1‑149 (MQ=255)
                                  gCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGa                                                                                                                              <  2:3768/136‑1 (MQ=255)
                                    cTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGtt                                                                                                                                         >  3:379841/1‑123 (MQ=255)
                                      ggTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGAtttt                                                                                                             >  3:851682/1‑149 (MQ=255)
                                         aTCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTa                                                                                                          <  3:1414014/149‑1 (MQ=255)
                                         aTCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTa                                                                                                          >  4:1414014/1‑149 (MQ=255)
                                          tCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGaa                                                                                                                                <  2:776435/126‑1 (MQ=255)
                                            tGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGtttt                                                                                                                                       >  1:317320/1‑117 (MQ=255)
                                            tGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGtttt                                                                                                                                       <  2:317320/117‑1 (MQ=255)
                                             gATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGaa                                                                                                      >  2:213497/1‑149 (MQ=255)
                                              aTGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGtt                                                                                                                                         >  1:996359/1‑113 (MQ=255)
                                                ggCTCGCGCAGAAAAACAGCGGATACGCGACATACAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAAc                                                                                                   >  4:293299/1‑149 (MQ=255)
                                                   tCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGAttt                                                                                                              <  3:311040/135‑1 (MQ=255)
                                                    cgcgCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCt                                                                                               <  2:883192/149‑1 (MQ=255)
                                                      cgcAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTc                                                                                             <  3:122958/149‑1 (MQ=255)
                                                      cgcAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTc                                                                                             <  3:902956/149‑1 (MQ=255)
                                                        cAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCAt                                                                                           >  2:149936/1‑149 (MQ=255)
                                                           aaaaaCAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCAtctc                                                                                        <  2:861447/149‑1 (MQ=255)
                                                                    ggATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGcc                                                                               <  4:63421/149‑1 (MQ=255)
                                                                    gaaTACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGcc                                                                               >  4:281508/3‑149 (MQ=255)
                                                                     gATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGtt                                                                                                                     <  1:1051186/110‑1 (MQ=255)
                                                                     gATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCt                                                                              >  1:68091/1‑149 (MQ=255)
                                                                      aTACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTg                                                                             >  3:1403078/1‑149 (MQ=255)
                                                                          gcgACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAAtg                                                                         >  2:132180/1‑149 (MQ=255)
                                                                            gACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTTGCGGCATCGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCGTGACtgtg                                                                       >  1:1152660/1‑149 (MQ=255)
                                                                            gACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAAtgtg                                                                       >  3:736610/1‑149 (MQ=255)
                                                                               aTCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCt                                                                    <  2:1366795/149‑1 (MQ=255)
                                                                                 ccAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTgc                                                                  >  3:670606/1‑149 (MQ=255)
                                                                                  cAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTgcg                                                                 >  3:510579/1‑149 (MQ=255)
                                                                                     cGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTg                                                              <  3:5666/149‑1 (MQ=255)
                                                                                          aaGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGtttt                                                                                                                                       >  4:1268030/1‑71 (MQ=255)
                                                                                           aGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAg                                                        <  3:293299/149‑1 (MQ=255)
                                                                                           aGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAg                                                        >  2:1153600/1‑149 (MQ=255)
                                                                                            gACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAgg                                                       >  1:406334/1‑149 (MQ=255)
                                                                                                  gCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTggcgg                                                 >  3:1134955/1‑149 (MQ=255)
                                                                                                       aGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTc                                            <  2:993981/149‑1 (MQ=255)
                                                                                                        gACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCg                                           >  1:521711/1‑149 (MQ=255)
                                                                                                        gACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCg                                           >  4:1120023/1‑149 (MQ=255)
                                                                                                           acTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGcta                                        >  2:429195/1‑147 (MQ=255)
                                                                                                             tAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCtt                                                                                              <  1:90660/93‑1 (MQ=255)
                                                                                                             tAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCtt                                                                                              >  2:90660/1‑93 (MQ=255)
                                                                                                             tAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGc                                                                      >  4:84948/1‑117 (MQ=255)
                                                                                                             tAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCatt                                      >  1:1050133/1‑149 (MQ=255)
                                                                                                             tAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCatt                                      >  1:818262/1‑149 (MQ=255)
                                                                                                             tAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGAGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGCTGGCGGCGCTCGCCatt                                      >  2:1067878/1‑149 (MQ=255)
                                                                                                              aaaaTTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCatta                                     <  3:352393/149‑1 (MQ=255)
                                                                                                                 aTTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCTCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCAttatta                                  <  4:1391243/149‑1 (MQ=255)
                                                                                                                 aTTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCAttatta                                  >  1:1167874/1‑149 (MQ=255)
                                                                                                                  tttGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATtattat                                 <  4:1134955/149‑1 (MQ=255)
                                                                                                                     gCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGAt                              <  3:598505/149‑1 (MQ=255)
                                                                                                                        aaCCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCg                           >  3:838297/1‑149 (MQ=255)
                                                                                                                         aCCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGt                          <  2:295448/149‑1 (MQ=255)
                                                                                                                         aCCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGt                          <  3:180822/149‑1 (MQ=255)
                                                                                                                           cTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTgg                        >  1:854376/1‑149 (MQ=255)
                                                                                                                           cTGGCTACATAATGCCGCGCATGACGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTgg                        <  3:1231356/149‑1 (MQ=255)
                                                                                                                            tGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGATGAAACGTTGGGATTTTTTAAGTAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGc                       <  4:837754/149‑1 (MQ=255)
                                                                                                                            tGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGc                       >  1:464408/1‑149 (MQ=255)
                                                                                                                             ggCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGcc                      >  1:1293587/1‑149 (MQ=255)
                                                                                                                                tACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCa                                                         >  4:1215326/1‑111 (MQ=255)
                                                                                                                                tACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCg                   >  1:1397518/1‑149 (MQ=255)
                                                                                                                                tACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCg                   >  1:123926/1‑149 (MQ=255)
                                                                                                                                  cATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACAt                                                                                                 >  3:1179511/1‑69 (MQ=255)
                                                                                                                                  cATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGAc                 >  4:882166/1‑149 (MQ=255)
                                                                                                                                   aTAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACg                >  3:1331999/1‑149 (MQ=255)
                                                                                                                                     aaTGCCGCGACATGCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTg              >  2:1074486/1‑149 (MQ=255)
                                                                                                                                     aaTGCCGCGACATGCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTg              >  4:718743/1‑149 (MQ=255)
                                                                                                                                     aaTGCCGCGACATGCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTg              >  2:1425104/1‑149 (MQ=255)
                                                                                                                                      aTGCCGCGACATGCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGc             >  2:583608/1‑149 (MQ=255)
                                                                                                                                       tGCCGCGACATGCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCt            >  1:945979/1‑149 (MQ=255)
                                                                                                                                       tGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCt            >  1:576290/1‑149 (MQ=255)
                                                                                                                                        gCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCtg           >  3:439706/1‑149 (MQ=255)
                                                                                                                                          cgcgCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCtgat         <  1:65697/149‑1 (MQ=255)
                                                                                                                                               cATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCTGATGAtt     <  4:332049/149‑1 (MQ=255)
                                                                                                                                               cATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCTGATGAtt     <  4:544223/149‑1 (MQ=255)
                                                                                                                                                aTGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGc                       <  3:499974/130‑1 (MQ=255)
                                                                                                                                                aTGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGc                       >  4:499974/1‑130 (MQ=255)
                                                                                                                                                 tGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCTGATGAtttt   <  3:1120023/149‑1 (MQ=255)
                                                                                                                                                  gTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGTGCTTGTGCAGGTGGCGGCGCTTGCCATTATTATGATCCGTGGCCTCGACGTGCTGATGATTTTc  >  2:165014/1‑149 (MQ=255)
                                                                                                                                                  gTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCTGATGATTTTc  <  4:196743/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GATCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGCATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGCTGATGATTTTC  >  NC_000913/897827‑898120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: