Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,390,012 Δ1 bp 8.8% intergenic (+48/+36) yfbP → / ← nuoN TPR‑like repeats‑containing protein/NADH:ubiquinone oxidoreductase, membrane subunit N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,390,0120G. 8.8% 462.0 / 38.2 136intergenic (+48/+36)yfbP/nuoNTPR‑like repeats‑containing protein/NADH:ubiquinone oxidoreductase, membrane subunit N
Reads supporting (aligned to +/- strand):  ref base G (64/60);  new base . (9/3);  total (73/63)
Fisher's exact test for biased strand distribution p-value = 1.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.21e-01

Only 100 of 137 total aligned reads displayed.

ATTTCAGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACT  >  NC_000913/2389865‑2390160
                                                                                                                                                   |                                                                                                                                                       
aTTTCAGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGc                                                                                                                                                        >  3:1376431/1‑149 (MQ=255)
 tttCAGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCa                                                                                                                                                       >  3:504742/1‑149 (MQ=255)
 tttCAGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCa                                                                                                                                                       <  4:1161006/149‑1 (MQ=255)
  ttCAGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCAt                                                                                                                                                      >  1:749839/1‑149 (MQ=255)
  ttCAGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCAt                                                                                                                                                      <  3:894354/149‑1 (MQ=255)
     aGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTc                                                                                                                                                  >  2:1474909/1‑149 (MQ=255)
      ggAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTcg                                                                                                                                                 >  4:186676/1‑149 (MQ=255)
            cTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGCGTATTTTGACGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTGTTAAGAAAAACCGCCGACATGCTcggcggtt                                                                                                                                           <  4:894955/149‑3 (MQ=255)
             tATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCtt                                                                                                                                        >  3:1008760/1‑149 (MQ=255)
              aTGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCttt                                                                                                                                       <  4:870858/149‑1 (MQ=255)
                gATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTCAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCt                                                                                                                                     <  4:101604/149‑1 (MQ=255)
                  ttttAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGa                                                                                                                                   >  3:1121417/1‑149 (MQ=255)
                         atCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACaa                                                                                                                            >  3:63073/1‑149 (MQ=255)
                          tCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGAc                                                                                                                                  <  4:476148/142‑1 (MQ=255)
                          tCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGAc                                                                                                                                  >  3:476148/1‑142 (MQ=255)
                           cGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAg                                                                                                                          <  4:159712/149‑1 (MQ=255)
                            gATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGt                                                                                                                         <  2:568052/149‑1 (MQ=255)
                             aTGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTa                                                                                                                        <  1:443068/149‑1 (MQ=255)
                              tGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCCACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTaa                                                                                                                       <  2:1416423/149‑1 (MQ=255)
                              tGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCAATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTaa                                                                                                                       <  2:801375/149‑1 (MQ=255)
                                  tGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGa                                                                                                                   <  2:104954/149‑1 (MQ=255)
                                      gggCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTAc                                                                                                               >  2:465237/1‑149 (MQ=255)
                                        gCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACAt                                                                                                             >  1:1181900/1‑149 (MQ=255)
                                         cAAAATTCAGGCAGGTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATc                                                                                                            >  2:172806/1‑149 (MQ=255)
                                          aaaaTTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACaa                                                                                                                            <  2:1204453/132‑1 (MQ=255)
                                          aaaaTTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACaa                                                                                                                            >  1:1204453/1‑132 (MQ=255)
                                              ttCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCgg                                                                                                       >  2:1335276/1‑149 (MQ=255)
                                                 aGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTaa                                                                                                                       >  1:853484/1‑130 (MQ=255)
                                                 aGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTaa                                                                                                                       <  2:853484/130‑1 (MQ=255)
                                                 aGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCAt                                                                                                    >  4:461139/1‑149 (MQ=255)
                                                  ggCAGTTAGGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTCTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTCACCGATTACATCAGCGGCAtt                                                                                                   <  2:260253/149‑1 (MQ=255)
                                                      gTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCa                                                                                               <  1:302975/149‑1 (MQ=255)
                                                           gTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGc                                                                                          <  1:1429147/149‑1 (MQ=255)
                                                                gagTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAAt                                                                                     <  2:534006/149‑1 (MQ=255)
                                                                  gTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGc                                                                                   <  2:259849/149‑1 (MQ=255)
                                                                          gCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGc                                                                           <  1:786453/149‑1 (MQ=255)
                                                                            gATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCgg                                                                         <  1:1033263/149‑1 (MQ=255)
                                                                              tAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGtt                                                                       <  2:300210/149‑1 (MQ=255)
                                                                              tAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGtt                                                                       <  3:7079/149‑1 (MQ=255)
                                                                              tAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGtt                                                                       <  3:461139/149‑1 (MQ=255)
                                                                                gTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTtgt                                                                     <  1:1277872/149‑1 (MQ=255)
                                                                                 tCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAAt                                                                               <  4:1351222/138‑1 (MQ=255)
                                                                                 tCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAAt                                                                               >  3:1351222/1‑138 (MQ=255)
                                                                                 tCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCa                                                                             >  3:1060992/1‑140 (MQ=255)
                                                                                 tCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCa                                                                             <  4:1060992/140‑1 (MQ=255)
                                                                                 tCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGc                                                                           >  1:914887/1‑142 (MQ=255)
                                                                                 tCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTtgtg                                                                    >  3:481236/1‑149 (MQ=255)
                                                                                  cGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTgg                                                                   <  2:628419/149‑1 (MQ=255)
                                                                                          gTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATacac                                                           >  1:1081042/1‑149 (MQ=255)
                                                                                          gTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATacac                                                           >  2:639985/1‑149 (MQ=255)
                                                                                             cACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCa                                                        >  3:381611/1‑149 (MQ=255)
                                                                                              aCTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAg                                                       >  4:1174579/1‑149 (MQ=255)
                                                                                               cTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGc                                                      >  2:702265/1‑149 (MQ=255)
                                                                                                ttAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAAt                                                                               >  4:1319991/1‑123 (MQ=255)
                                                                                                ttAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAAt                                                                               <  3:1319991/123‑1 (MQ=255)
                                                                                                  aaCGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCAcc                                                   >  1:1020505/1‑149 (MQ=255)
                                                                                                     gCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCa                                                     <  4:671068/144‑1 (MQ=255)
                                                                                                       tctGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACa                                                                                                              <  3:487427/85‑1 (MQ=255)
                                                                                                         tGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCgg                                                                                                       <  1:654769/90‑1 (MQ=255)
                                                                                                              ctatctaCTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGt                                       <  3:1172885/149‑1 (MQ=255)
                                                                                                                 tctaCTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCa                                    >  1:993959/1‑149 (MQ=255)
                                                                                                                 tctaCTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCa                                    <  4:459118/149‑1 (MQ=255)
                                                                                                                   tactaTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGTGGTtgt                                                                     <  4:476562/114‑1 (MQ=255)
                                                                                                                     ctaTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAgaga                                <  2:468145/149‑1 (MQ=255)
                                                                                                                      taTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGAt                               <  1:489069/149‑1 (MQ=255)
                                                                                                                        tCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCa                             <  3:911179/149‑1 (MQ=255)
                                                                                                                         cccTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAg                            <  1:1335276/149‑1 (MQ=255)
                                                                                                                          ccTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTCATCAGCGGTTGTGGCCATACACCCAGCACCAGTATCAACAGTGCAGAGATCAGc                           <  4:1008760/149‑1 (MQ=255)
                                                                                                                          ccTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGc                           >  2:581131/1‑149 (MQ=255)
                                                                                                                           cttcttTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGca                          >  3:10539/1‑149 (MQ=255)
                                                                                                                           cttcttTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGca                          >  3:898492/1‑149 (MQ=255)
                                                                                                                            ttcttTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACa                                                                                                                     >  4:141320/1‑57 (MQ=255)
                                                                                                                            ttcttTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACa                                                                                                                     <  3:141320/57‑1 (MQ=255)
                                                                                                                            ttcttTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGcac                         >  4:1262560/1‑149 (MQ=255)
                                                                                                                            ttcttTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGcac                         >  1:1467016/1‑149 (MQ=255)
                                                                                                                            ttcttTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGcac                         >  2:359148/1‑149 (MQ=255)
                                                                                                                               tttttCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGTACCAGTACCAACAGTGCAGAGATCAGCACAAc                      >  2:607636/1‑149 (MQ=255)
                                                                                                                               tttttCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAAc                      >  3:31351/1‑149 (MQ=255)
                                                                                                                                 tttCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGa                    >  3:461839/1‑149 (MQ=255)
                                                                                                                                    tcagaaagCCGCCGACATGCTCGGCGGTTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATa                  >  2:1227518/5‑149 (MQ=38)
                                                                                                                                     aGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCaa                                           <  4:765737/122‑1 (MQ=255)
                                                                                                                                      gAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCa                                                  <  1:1280633/114‑1 (MQ=255)
                                                                                                                                      gAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccg               >  2:828689/1‑149 (MQ=255)
                                                                                                                                       gaaagCCGCCGACATGCTCGGCGGTTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccg               >  2:1023111/2‑149 (MQ=255)
                                                                                                                                       gaaagCCGCCGACATGCTCGGCGGTTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccg               >  3:763002/2‑149 (MQ=255)
                                                                                                                                       gaaagCCGCCGACATGCTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccgc              >  2:34576/2‑149 (MQ=255)
                                                                                                                                        aaagccgccgACATGCTCGGCGGTTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCa                                    <  2:1196107/123‑1 (MQ=38)
                                                                                                                                        aaagCCGCCGACATGCTCGGCGGTTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCa                                    >  1:1196107/1‑127 (MQ=38)
                                                                                                                                         aagccgccgACATGCTCGGCGGTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAg                            <  2:727981/130‑1 (MQ=255)
                                                                                                                                         aagccgccgACATGCTCGGCGGTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAg                            >  1:727981/4‑133 (MQ=255)
                                                                                                                                          agccgccgACATGCTCGGCGGTTTTTCTGACTTACAAAGTAACAGCTTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccgccc            >  3:1437786/3‑149 (MQ=255)
                                                                                                                                          aaCCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAAc                      <  4:702937/138‑1 (MQ=255)
                                                                                                                                          aaCCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAAc                      >  3:702937/1‑138 (MQ=255)
                                                                                                                                            ccgccgACATGCTCGGCGGTTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccgcccgc          >  1:275520/1‑149 (MQ=255)
                                                                                                                                               ccgAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTg      >  4:483404/1‑149 (MQ=255)
                                                                                                                                                cgAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGt     <  2:732462/149‑1 (MQ=255)
                                                                                                                                                 gAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTa    >  2:1480563/1‑149 (MQ=255)
                                                                                                                                                 gAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTa    <  3:172260/149‑1 (MQ=255)
                                                                                                                                                  aGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTAc   <  2:1108480/149‑1 (MQ=255)
                                                                                                                                                   gCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACt  <  3:483404/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                       
ATTTCAGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACT  >  NC_000913/2389865‑2390160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: