Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,679,430 Δ1 bp 6.5% intergenic (‑63/+34) yphF ← / ← yphG putative sugar ABC transporter periplasmic binding protein/DUF4380 domain‑containing TPR repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,679,4300G. 6.5% 425.0 / 23.1 123intergenic (‑63/+34)yphF/yphGputative sugar ABC transporter periplasmic binding protein/DUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand):  ref base G (58/57);  new base . (2/6);  total (60/63)
Fisher's exact test for biased strand distribution p-value = 2.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.26e-01

Only 100 of 125 total aligned reads displayed.

TTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCC  >  NC_000913/2679283‑2679578
                                                                                                                                                   |                                                                                                                                                     
tttCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAgc                                                                                                                                                      >  3:104536/1‑149 (MQ=255)
    cGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGt                                                                                                                                                 <  3:678829/149‑1 (MQ=255)
     gCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTg                                                                                                                                                >  3:203782/1‑149 (MQ=255)
         cccTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGc                                                                                                                                            <  2:1085973/149‑1 (MQ=255)
          ccTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCt                                                                                                                                           >  1:985750/1‑149 (MQ=255)
          ccTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCt                                                                                                                                           <  1:1392429/149‑1 (MQ=255)
          ccTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCACGCGCTGCCGCt                                                                                                                                           <  2:913901/149‑1 (MQ=255)
           cTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCACGCGCTGCCGCTc                                                                                                                                          <  2:395804/149‑1 (MQ=255)
            tGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCa                                                                                                                                         >  3:366663/1‑149 (MQ=255)
            tGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCa                                                                                                                                         <  3:660166/149‑1 (MQ=255)
            tGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCACGCGCTGCCGCTCa                                                                                                                                         <  3:451702/149‑1 (MQ=255)
               cAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCACGCGCTGCCGCTCatca                                                                                                                                      <  4:734310/149‑4 (MQ=255)
                aaaTAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAata                                                                                                                                     >  2:336322/1‑149 (MQ=255)
                   tAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTa                                                                                                                                  <  1:388865/149‑1 (MQ=255)
                     gTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACa                                                                                                                                <  2:129305/149‑1 (MQ=255)
                     gTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACa                                                                                                                                <  2:384553/149‑1 (MQ=255)
                     gTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACa                                                                                                                                <  3:500775/149‑1 (MQ=255)
                     gTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACa                                                                                                                                <  1:10175/149‑1 (MQ=255)
                     gTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACa                                                                                                                                <  1:1221160/149‑1 (MQ=255)
                       gcgcTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGt                                                                                                                              <  3:1048749/149‑1 (MQ=255)
                        cgcTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGtt                                                                                                                             <  4:758186/149‑1 (MQ=255)
                           tACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAAt                                                                                                                          >  2:1391528/1‑149 (MQ=255)
                             ccTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCTTAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAg                                                                                                                        <  1:259157/149‑1 (MQ=255)
                             ccTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGACGCTCAAATATTAGAGTTAATAg                                                                                                                        >  3:1230517/1‑149 (MQ=255)
                              cTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAgg                                                                                                                       >  3:611980/1‑149 (MQ=255)
                              cTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAgg                                                                                                                       >  1:951049/1‑149 (MQ=255)
                               tAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTa                                                                                                                                  <  3:1398653/137‑1 (MQ=255)
                               tAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTa                                                                                                                                  >  4:1398653/1‑137 (MQ=255)
                               tAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCCGCAGCGCGTGCCGCTCAAATATTACAGTTAATAggg                                                                                                                      <  4:1230517/149‑1 (MQ=255)
                                aaaaGCGTAGCCATTAATAATAGATTACGGGTTGATCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTACTAGAGt                                                                                                                     >  3:971409/4‑149 (MQ=255)
                                   aGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTa                                                                                                                  <  1:1391528/149‑1 (MQ=255)
                                      gTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTg                                                                                                               >  1:845721/1‑149 (MQ=255)
                                      gTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTg                                                                                                               >  4:565659/1‑149 (MQ=255)
                                       tAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGa                                                                                                              >  3:1337096/1‑149 (MQ=255)
                                           cATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAAcgc                                                                                                          <  1:1349103/149‑1 (MQ=255)
                                            aTTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAAcgcg                                                                                                         >  3:253850/1‑149 (MQ=255)
                                            aTTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGAGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAAcgcg                                                                                                         >  1:1127215/1‑149 (MQ=255)
                                             ttAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCgg                                                                                                        >  4:477354/1‑149 (MQ=255)
                                             ttAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAACCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCgg                                                                                                        <  2:656653/149‑1 (MQ=255)
                                               aataatAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTGATAGGGTTTAGTGAACGCGGtt                                                                                                      <  2:1127215/149‑1 (MQ=255)
                                                ataataGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTa                                                                                                     >  2:451840/1‑149 (MQ=255)
                                                  aataGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTaaa                                                                                                   >  3:1212651/1‑149 (MQ=255)
                                                   ataGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTaaaa                                                                                                  >  4:1115028/1‑149 (MQ=255)
                                                      gATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAGATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATa                                                                                               >  4:874642/1‑149 (MQ=255)
                                                        ttACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATaaa                                                                                             <  3:789090/149‑1 (MQ=255)
                                                        ttACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATaaa                                                                                             <  4:1337096/149‑1 (MQ=255)
                                                         tACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATaaaa                                                                                            <  3:34898/149‑1 (MQ=255)
                                                             ggTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCa                                                                                        <  3:434371/149‑1 (MQ=255)
                                                              gttgttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTaa                                                                                                                           <  3:720246/113‑1 (MQ=255)
                                                              gttgttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTaa                                                                                                                           >  4:720246/1‑113 (MQ=255)
                                                                    ctcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggt                                                                                 >  2:864619/1‑149 (MQ=255)
                                                                    ctcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggt                                                                                 >  4:1412932/1‑149 (MQ=255)
                                                                     tcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAAt                                                                                                >  4:121677/1‑133 (MQ=255)
                                                                     tcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAAt                                                                                                <  3:121677/133‑1 (MQ=255)
                                                                     tcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACgtgg                                                                                >  4:1244868/1‑146 (MQ=255)
                                                                     tcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggtg                                                                                >  1:192556/1‑149 (MQ=255)
                                                                     tcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggtg                                                                                >  4:637786/1‑149 (MQ=255)
                                                                     tcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggtg                                                                                <  2:679063/149‑1 (MQ=255)
                                                                       aTTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggtggt                                                                              >  1:956572/1‑149 (MQ=255)
                                                                        tttttGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGgtggtg                                                                             <  2:1259298/149‑1 (MQ=255)
                                                                                ggCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGc                                                                     <  2:762742/149‑1 (MQ=255)
                                                                                   aTAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAAt                                                                                                                          <  2:1253800/93‑1 (MQ=255)
                                                                                   aTAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAAt                                                                                                                          >  1:1253800/1‑93 (MQ=255)
                                                                                   aTAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGc                                                                  >  3:1299016/1‑149 (MQ=255)
                                                                                   aTAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGc                                                                  >  3:643444/1‑149 (MQ=255)
                                                                                   aTAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGc                                                                  >  4:639194/1‑149 (MQ=255)
                                                                                   aTAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGc                                                                  >  2:1112779/1‑149 (MQ=255)
                                                                                         cctcctGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCgg                                                            >  1:467789/1‑149 (MQ=255)
                                                                                         cctcctGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCga                                                            >  1:1040615/1‑148 (MQ=255)
                                                                                               gTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGc                                                      >  2:1378711/1‑149 (MQ=255)
                                                                                                tAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCAACACGGTGGTGAATAATGCCGCTTTCGGCCAAGcc                                                     >  1:431929/1‑149 (MQ=255)
                                                                                                 aGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCg                                                    <  1:1303024/149‑1 (MQ=255)
                                                                                                 aGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCg                                                    >  3:1291489/1‑149 (MQ=255)
                                                                                                  gggTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGTAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCgg                                                   >  3:1040095/1‑149 (MQ=255)
                                                                                                        ttattaACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAAt                                             <  1:158155/149‑1 (MQ=255)
                                                                                                           ttaACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGc                                                                     >  1:1015929/1‑122 (MQ=255)
                                                                                                           ttaACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGc                                                                     <  2:1015929/122‑1 (MQ=255)
                                                                                                            taACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGcc                                         <  2:1176365/149‑1 (MQ=255)
                                                                                                                aacGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCa                                     >  4:44983/1‑149 (MQ=255)
                                                                                                                aacGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCa                                     >  3:847340/1‑149 (MQ=255)
                                                                                                                 acGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCaa                                    <  4:1044906/149‑1 (MQ=255)
                                                                                                                  cGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAAt                                   <  4:366663/149‑1 (MQ=255)
                                                                                                                     cTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGaa                                                                                                             <  3:115426/72‑1 (MQ=255)
                                                                                                                      ttATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAg                               >  2:845710/1‑149 (MQ=255)
                                                                                                                           cTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGa                          <  3:477354/149‑1 (MQ=255)
                                                                                                                           cTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGa                          <  4:564955/149‑1 (MQ=255)
                                                                                                                           cTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGa                          <  4:413585/149‑1 (MQ=255)
                                                                                                                           cTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGa                          <  1:1378711/149‑1 (MQ=255)
                                                                                                                            tAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAg                         >  3:1084124/1‑149 (MQ=255)
                                                                                                                             aaTTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGc                        <  4:104536/149‑1 (MQ=255)
                                                                                                                              attattTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCa                       >  1:636153/1‑149 (MQ=255)
                                                                                                                                  ttttGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt                   >  2:1471657/1‑149 (MQ=255)
                                                                                                                                      gtgATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCata               >  1:1281274/1‑149 (MQ=255)
                                                                                                                                          tgaGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTgc           <  3:114057/149‑1 (MQ=255)
                                                                                                                                           gaGCGGCACGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGa                                                  >  4:1043164/1‑109 (MQ=255)
                                                                                                                                           gaGCGGCACGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTgcg          >  4:1198116/1‑149 (MQ=255)
                                                                                                                                             gCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCa        <  2:956572/149‑1 (MQ=255)
                                                                                                                                               ggCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAAc      <  2:153359/149‑1 (MQ=255)
                                                                                                                                                gCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAAcc     <  1:1440854/149‑1 (MQ=255)
                                                                                                                                                   gcgcGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTcc  >  4:231670/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCC  >  NC_000913/2679283‑2679578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: