Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,390,016:1 +C 9.2% intergenic (+52/+32) yfbP → / ← nuoN TPR‑like repeats‑containing protein/NADH:ubiquinone oxidoreductase, membrane subunit N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,390,0161.C 9.2% 525.0 / 53.9 130intergenic (+52/+32)yfbP/nuoNTPR‑like repeats‑containing protein/NADH:ubiquinone oxidoreductase, membrane subunit N
Reads supporting (aligned to +/- strand):  ref base . (59/59);  new base C (9/3);  total (68/62)
Fisher's exact test for biased strand distribution p-value = 1.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-01

Only 100 of 130 total aligned reads displayed.

AGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACT  >  NC_000913/2389870‑2390160
                                                                                                                                                   |                                                                                                                                                  
aGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTc                                                                                                                                                  >  2:1474909/1‑149 (MQ=255)
 ggAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTcg                                                                                                                                                 >  4:186676/1‑149 (MQ=255)
   aaCGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTcggc                                                                                                                                               <  1:1402181/149‑1 (MQ=255)
    aCGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTcggcg                                                                                                                                              >  3:1406946/1‑149 (MQ=255)
       cTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGCGTATTTTGACGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTGTTAAGAAAAACCGCCGACATGCTcggcggtt                                                                                                                                           <  4:894955/149‑3 (MQ=255)
         aTGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCttt                                                                                                                                       <  4:870858/149‑1 (MQ=255)
                    atCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACaa                                                                                                                            >  3:63073/1‑149 (MQ=255)
                     tCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGAc                                                                                                                                  <  4:476148/142‑1 (MQ=255)
                     tCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGAc                                                                                                                                  >  3:476148/1‑142 (MQ=255)
                      cGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAg                                                                                                                          <  4:159712/149‑1 (MQ=255)
                      cGATGAATGGCAGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGTCTTTCTGACTTACAAAg                                                                                                                          <  4:1220256/149‑1 (MQ=255)
                       gATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGt                                                                                                                         <  2:568052/149‑1 (MQ=255)
                        aTGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTa                                                                                                                        <  1:443068/149‑1 (MQ=255)
                         tGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTaa                                                                                                                       <  1:842743/149‑1 (MQ=255)
                         tGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCCACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTaa                                                                                                                       <  2:1416423/149‑1 (MQ=255)
                         tGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCAATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTaa                                                                                                                       <  2:801375/149‑1 (MQ=255)
                             tGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGa                                                                                                                   <  2:104954/149‑1 (MQ=255)
                                 gggCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTAc                                                                                                               >  2:465237/1‑149 (MQ=255)
                                   gCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACAt                                                                                                             >  1:1181900/1‑149 (MQ=255)
                                    cAAAATTCAGGCAGGTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATc                                                                                                            >  2:172806/1‑149 (MQ=255)
                                     aaaaTTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACaa                                                                                                                            <  2:1204453/132‑1 (MQ=255)
                                     aaaaTTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACaa                                                                                                                            >  1:1204453/1‑132 (MQ=255)
                                       aaTTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGc                                                                                                         <  4:380499/149‑1 (MQ=255)
                                         ttCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCgg                                                                                                       >  2:1335276/1‑149 (MQ=255)
                                            aGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTaa                                                                                                                       <  2:853484/130‑1 (MQ=255)
                                            aGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTaa                                                                                                                       >  1:853484/1‑130 (MQ=255)
                                            aGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCAt                                                                                                    >  4:461139/1‑149 (MQ=255)
                                             ggCAGTTAGGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTCTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTCACCGATTACATCAGCGGCAtt                                                                                                   <  2:260253/149‑1 (MQ=255)
                                               cAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGc                                                                                                 <  4:1376431/149‑1 (MQ=255)
                                                 gTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCa                                                                                               <  1:302975/149‑1 (MQ=255)
                                                      gTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGc                                                                                          <  1:1429147/149‑1 (MQ=255)
                                                       tttAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGca                                                                                         >  2:20647/1‑149 (MQ=255)
                                                           gagTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAAt                                                                                     <  2:534006/149‑1 (MQ=255)
                                                             gTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGc                                                                                   >  2:627770/1‑149 (MQ=255)
                                                                     gCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGc                                                                           <  1:786453/149‑1 (MQ=255)
                                                                       gATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCgg                                                                         <  1:1033263/149‑1 (MQ=255)
                                                                         tAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGtt                                                                       <  2:300210/149‑1 (MQ=255)
                                                                         tAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGtt                                                                       <  3:7079/149‑1 (MQ=255)
                                                                         tAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGtt                                                                       <  3:461139/149‑1 (MQ=255)
                                                                           gTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTtgt                                                                     <  1:1277872/149‑1 (MQ=255)
                                                                            tCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAAt                                                                               <  4:1351222/138‑1 (MQ=255)
                                                                            tCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCa                                                                             <  4:1060992/140‑1 (MQ=255)
                                                                            tCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCa                                                                             >  3:1060992/1‑140 (MQ=255)
                                                                            tCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGc                                                                           <  2:914887/142‑1 (MQ=255)
                                                                            tCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTtgtg                                                                    >  3:481236/1‑149 (MQ=255)
                                                                             cGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTgg                                                                   <  2:555531/149‑1 (MQ=255)
                                                                             cGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTgg                                                                   <  2:628419/149‑1 (MQ=255)
                                                                                acacAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCa                                                                <  4:1121417/149‑1 (MQ=255)
                                                                                     gTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATacac                                                           >  1:1081042/1‑149 (MQ=255)
                                                                                     gTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATacac                                                           >  2:639985/1‑149 (MQ=255)
                                                                                        cACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCa                                                        >  3:381611/1‑149 (MQ=255)
                                                                                         aCTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAg                                                       >  4:1174579/1‑149 (MQ=255)
                                                                                          cTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGc                                                      >  2:702265/1‑149 (MQ=255)
                                                                                           ttAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAAt                                                                               >  4:1319991/1‑123 (MQ=255)
                                                                                           ttAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAAt                                                                               <  3:1319991/123‑1 (MQ=255)
                                                                                           ttAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCa                                                     >  3:49946/1‑149 (MQ=255)
                                                                                           ttAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCa                                                     >  3:1085226/1‑149 (MQ=255)
                                                                                             aaCGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCAcc                                                   >  1:1020505/1‑149 (MQ=255)
                                                                                                gCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCa                                                     <  4:671068/144‑1 (MQ=255)
                                                                                                gCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCa                                                     >  3:671068/1‑144 (MQ=255)
                                                                                                  tctGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACa                                                                                                              >  4:487427/1‑85 (MQ=255)
                                                                                                  tctGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACa                                                                                                              <  3:487427/85‑1 (MQ=255)
                                                                                                   ctGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTAcc                                             <  2:1125322/149‑1 (MQ=255)
                                                                                                    tGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCgg                                                                                                       <  1:654769/90‑1 (MQ=255)
                                                                                                       gTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACa                                         <  2:1020505/149‑1 (MQ=255)
                                                                                                         ctatctaCTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGt                                       <  3:1172885/149‑1 (MQ=255)
                                                                                                            tctaCTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCa                                    >  1:993959/1‑149 (MQ=255)
                                                                                                            tctaCTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCa                                    <  1:1474909/149‑1 (MQ=255)
                                                                                                              tactaTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGTGGTtgt                                                                     <  4:476562/114‑1 (MQ=255)
                                                                                                              tactaTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGTGGTtgt                                                                     >  3:476562/1‑114 (MQ=255)
                                                                                                               actaTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCagag                                 >  1:125136/1‑149 (MQ=255)
                                                                                                                 taTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGAt                               <  1:489069/149‑1 (MQ=255)
                                                                                                                   tCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCa                             <  3:911179/149‑1 (MQ=255)
                                                                                                                    cccTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAg                            <  1:1335276/149‑1 (MQ=255)
                                                                                                                     ccTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTCATCAGCGGTTGTGGCCATACACCCAGCACCAGTATCAACAGTGCAGAGATCAGc                           <  4:1008760/149‑1 (MQ=255)
                                                                                                                     ccTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGc                           >  2:581131/1‑149 (MQ=255)
                                                                                                                      cttcttTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGca                          >  3:10539/1‑149 (MQ=255)
                                                                                                                      cttcttTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGca                          >  2:361040/1‑149 (MQ=255)
                                                                                                                      cttcttTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACACTGCAGAGATCAGca                          >  3:1224578/1‑149 (MQ=255)
                                                                                                                       ttcttTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACa                                                                                                                     <  3:141320/57‑1 (MQ=255)
                                                                                                                       ttcttTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGcac                         >  2:359148/1‑149 (MQ=255)
                                                                                                                          tttttCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAAc                      >  3:31351/1‑149 (MQ=255)
                                                                                                                            tttCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGa                    >  4:1008437/1‑149 (MQ=255)
                                                                                                                                aGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCaa                                           >  3:765737/1‑122 (MQ=255)
                                                                                                                                 gAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCa                                                  >  2:1280633/1‑114 (MQ=255)
                                                                                                                                 gAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccg               >  2:828689/1‑149 (MQ=255)
                                                                                                                                 gAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccg               >  2:34300/1‑149 (MQ=255)
                                                                                                                                  gaaagCCGCCGACATGCTCGGCGGTTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccg               >  2:1023111/2‑149 (MQ=255)
                                                                                                                                  gaaagCCGCCGACATGCTCGGCGGTTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccg               >  3:763002/2‑149 (MQ=255)
                                                                                                                                   aaagccgccgACATGCTCGGCGGTTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCa                                    <  2:1196107/123‑1 (MQ=38)
                                                                                                                                   aaagCCGCCGACATGCTCGGCGGTTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccgc              >  4:798811/1‑149 (MQ=255)
                                                                                                                                   aaaaCCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccgcc             <  4:461839/149‑1 (MQ=255)
                                                                                                                                    aagccgccgACATGCTCGGCGGTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAg                            <  2:727981/130‑1 (MQ=255)
                                                                                                                                    aagccgccgACATGCTCGGCGGTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAg                            >  1:727981/4‑133 (MQ=255)
                                                                                                                                     agccgccgACATGCTCGGCGGTTTTTCTGACTTACAAAGTAACAGCTTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccgccc            >  3:1437786/3‑149 (MQ=255)
                                                                                                                                     aaCCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAAc                      <  4:702937/138‑1 (MQ=255)
                                                                                                                                       ccgccgACATGCTCGGCGGTTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccgcccgc          >  1:275520/1‑149 (MQ=255)
                                                                                                                                           cgAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGt     <  2:732462/149‑1 (MQ=255)
                                                                                                                                            gAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTa    <  3:172260/149‑1 (MQ=255)
                                                                                                                                            gAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTa    >  2:1480563/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACT  >  NC_000913/2389870‑2390160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: