Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,572,928 C→G 5.9% A759P (GCA→CCA)  pqqL ← putative periplasmic M16 family zinc metalloendopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,572,9280CG 5.9% 599.7 / 23.1 169A759P (GCA→CCA) pqqLputative periplasmic M16 family zinc metalloendopeptidase
Reads supporting (aligned to +/- strand):  ref base C (71/88);  new base G (5/5);  total (76/93)
Fisher's exact test for biased strand distribution p-value = 7.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.87e-01

Only 100 of 172 total aligned reads displayed.

GAAGAAACGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTA  >  NC_000913/1572780‑1573076
                                                                                                                                                    |                                                                                                                                                    
gaagaaACGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGc                                                                                                                                                      <  1:1073257/149‑1 (MQ=255)
  agaaACGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCca                                                                                                                                                    <  1:769130/149‑1 (MQ=255)
    aaaCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCcaca                                                                                                                                                  >  2:608986/1‑149 (MQ=255)
    aaaCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCcaca                                                                                                                                                  >  3:91308/1‑149 (MQ=255)
    aaaCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCcaca                                                                                                                                                  >  1:511578/1‑149 (MQ=255)
     aaCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAg                                                                                                                                                 >  4:1272306/1‑149 (MQ=255)
      aCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAgg                                                                                                                                                <  4:408125/149‑1 (MQ=255)
      aCGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAgg                                                                                                                                                >  2:778429/1‑149 (MQ=255)
        gCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGtt                                                                                                                                              >  3:1089057/1‑149 (MQ=255)
         cTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGGCACAGGttt                                                                                                                                             <  3:507170/149‑2 (MQ=255)
           gTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGGCACAGGtttca                                                                                                                                           <  2:882367/149‑4 (MQ=255)
            tATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCAtt                                                                                                                                          >  1:776172/1‑149 (MQ=255)
             aTGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCAttt                                                                                                                                         <  3:759051/149‑1 (MQ=255)
              tGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCAtttt                                                                                                                                        <  3:1395220/149‑1 (MQ=255)
               gCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTg                                                                                                                                       <  1:596842/149‑1 (MQ=255)
                cTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGt                                                                                                                                      >  3:1059209/1‑149 (MQ=255)
                cTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGt                                                                                                                                      <  3:680293/149‑1 (MQ=255)
                 tCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGtt                                                                                                                                     >  3:791973/1‑149 (MQ=255)
                   cAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCcac                                                                                                                                                   >  2:1155429/1‑133 (MQ=255)
                   cAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCt                                                                                                                                   >  4:846810/1‑149 (MQ=255)
                    aGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCtt                                                                                                                                  <  4:310646/149‑1 (MQ=255)
                       tGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTa                                                                                                                               <  1:291784/149‑1 (MQ=255)
                       tGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTa                                                                                                                               <  1:608986/149‑1 (MQ=255)
                            gTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTa                                                                                                                          >  3:663734/1‑149 (MQ=255)
                             ttCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTaa                                                                                                                         <  2:1308682/149‑1 (MQ=255)
                              tCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAAc                                                                                                                        <  2:66385/149‑1 (MQ=255)
                                  gAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGAt                                                                                                                    >  1:125397/1‑149 (MQ=255)
                                  gAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGAt                                                                                                                    >  4:196523/1‑149 (MQ=255)
                                    atatTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGc                                                                                                                  <  4:1437049/149‑1 (MQ=255)
                                       ttAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGtt                                                                                                               >  2:285382/1‑149 (MQ=255)
                                       ttAACACGTAGATCTGTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGtt                                                                                                               >  4:1076077/1‑149 (MQ=255)
                                         aaCACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGt                                                                                                             >  1:1390751/1‑149 (MQ=255)
                                         aaCACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGt                                                                                                             >  2:719793/1‑149 (MQ=255)
                                            acGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCg                                                                                                          >  3:730282/1‑149 (MQ=255)
                                            acGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCg                                                                                                          >  4:1333077/1‑149 (MQ=255)
                                              gTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTc                                                                                                        >  2:1403684/1‑149 (MQ=255)
                                              gTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTc                                                                                                        >  3:496819/1‑149 (MQ=255)
                                              gTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTc                                                                                                        >  2:483169/1‑149 (MQ=255)
                                               tAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTcg                                                                                                       >  4:1109562/1‑149 (MQ=255)
                                                aGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTcgc                                                                                                      >  4:1300821/1‑149 (MQ=255)
                                                   tCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGTGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTcgcgcg                                                                                                   >  2:502188/1‑149 (MQ=255)
                                                     ttttGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAg                                                                                                 >  4:969852/1‑149 (MQ=255)
                                                     ttttGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAg                                                                                                 >  3:647005/1‑149 (MQ=255)
                                                              tGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGtt                                                                                        <  1:59493/149‑1 (MQ=255)
                                                                  aCGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCCGCGAGTTAATGGTTTACCt                                                                                   >  1:805717/1‑149 (MQ=255)
                                                                       aaaaGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCgg                                                                               >  3:542238/1‑149 (MQ=255)
                                                                           gCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTaa                                                                           <  3:1367203/149‑1 (MQ=255)
                                                                               cGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGc                                                                       >  1:506100/1‑149 (MQ=255)
                                                                                  gcgcCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGaa                                                                    <  3:11378/149‑1 (MQ=255)
                                                                                     ccATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAAACa                                                                 <  3:969852/149‑1 (MQ=255)
                                                                                      cATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTGCCTGCGGCTAATGGCGAATCag                                                                >  3:59116/1‑149 (MQ=255)
                                                                                      cATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCag                                                                <  3:995218/149‑1 (MQ=255)
                                                                                      cATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCag                                                                >  1:574752/1‑149 (MQ=255)
                                                                                       aTACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCaga                                                               <  3:989043/149‑1 (MQ=255)
                                                                                       aTACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCaga                                                               <  2:1077007/149‑1 (MQ=255)
                                                                                       aTACGCGACGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCaga                                                               <  3:1255598/149‑1 (MQ=255)
                                                                                         aCGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAgt                                                             <  3:263867/149‑1 (MQ=255)
                                                                                         aCGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAgt                                                             <  1:82195/149‑1 (MQ=255)
                                                                                         aCGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAgt                                                             <  4:791973/149‑1 (MQ=255)
                                                                                            cgTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTCTACCTGCGGCTAATGGCGAATCAGAGTGtt                                                          <  1:46439/149‑1 (MQ=255)
                                                                                             gTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGttt                                                         <  1:483169/149‑1 (MQ=255)
                                                                                               cGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTttga                                                       <  4:773075/149‑1 (MQ=255)
                                                                                                 gCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTttgatt                                                     >  1:1458864/1‑149 (MQ=255)
                                                                                                   aGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTttgattga                                                   >  3:386415/1‑149 (MQ=255)
                                                                                                    gATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTtgattgat                                                  <  4:647005/149‑1 (MQ=255)
                                                                                                    gATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTtgattgat                                                  >  1:238739/1‑149 (MQ=255)
                                                                                                    gATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTtgattgat                                                  <  3:1364016/149‑1 (MQ=255)
                                                                                                     aTTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATc                                                 <  2:1458864/149‑1 (MQ=255)
                                                                                                       tAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTttga                                                       <  1:329888/141‑1 (MQ=255)
                                                                                                         aCAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTaa                                             <  4:663734/149‑1 (MQ=255)
                                                                                                         aCAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTaa                                             <  3:146266/149‑1 (MQ=255)
                                                                                                             gtgtCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCaga                                                               <  4:1134282/127‑1 (MQ=255)
                                                                                                              tgtCCGGTAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAAc                                        >  2:807676/1‑149 (MQ=255)
                                                                                                                tCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAAcgc                                      <  3:1398771/149‑1 (MQ=255)
                                                                                                                tCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAAcgc                                      <  2:506100/149‑1 (MQ=255)
                                                                                                                    ggAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGtt                                                                                        <  4:692120/95‑1 (MQ=255)
                                                                                                                    ggAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTCTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTCCCTGCGGCTAATGGCGAATCAGAGTGTTTGATCGATCCTAAGTAGCGCGTaa                                  <  4:1236093/149‑1 (MQ=255)
                                                                                                                        tCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTaa                              >  1:1480381/1‑149 (MQ=255)
                                                                                                                         cATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAAc                             <  2:1275197/149‑1 (MQ=255)
                                                                                                                         cATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAAc                             <  3:1333077/149‑1 (MQ=255)
                                                                                                                         cATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAAc                             <  1:1156563/149‑1 (MQ=255)
                                                                                                                           tAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGc                           >  4:1071885/1‑149 (MQ=255)
                                                                                                                            aaCGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGcc                          >  1:1382495/1‑149 (MQ=255)
                                                                                                                            aaCGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGcc                          >  3:1118459/1‑149 (MQ=255)
                                                                                                                             aCGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGCCa                         <  1:719793/149‑1 (MQ=255)
                                                                                                                             aCGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGCCa                         >  1:1048398/1‑149 (MQ=255)
                                                                                                                                cTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGCCACGa                      <  4:1042187/149‑1 (MQ=255)
                                                                                                                                  tCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGCCACGAGt                    <  4:604952/149‑1 (MQ=255)
                                                                                                                                   ccACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGCCACGAGtt                   <  3:1409988/149‑1 (MQ=255)
                                                                                                                                     aCTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGCCACGAGTTTg                 >  4:256068/1‑149 (MQ=255)
                                                                                                                                        aatgaaCCTGTGGCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTaa                                             <  3:238988/114‑1 (MQ=255)
                                                                                                                                        aatgaaCCTGTGGCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGCCACGAGTTTGtct              >  3:1108691/5‑149 (MQ=255)
                                                                                                                                         tgAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGCCACGAGTTTGtctt             <  1:1181644/149‑1 (MQ=255)
                                                                                                                                         atgaaCCTGTGGCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGCCACGAGttt                  <  4:1247952/141‑1 (MQ=255)
                                                                                                                                         atgaaCCTGTGGCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGCCACGAGttt                  >  3:1247952/4‑144 (MQ=255)
                                                                                                                                           gaaCCTCTGGCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGCCACGAGTTTGtcttct           >  2:1064869/2‑149 (MQ=255)
                                                                                                                                           agaCCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGCCACGAGTTTGtcttct           >  2:332660/3‑149 (MQ=255)
                                                                                                                                            aaCCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTg          >  2:581288/1‑149 (MQ=255)
                                                                                                                                             aCCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGc         <  3:256068/149‑1 (MQ=255)
                                                                                                                                                    ccACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTa  >  2:180322/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GAAGAAACGCTGTATGCTCCAGATGCCTGTTCACGAATATTAACACGTAGATCTTTTGCCAGTGCGACGTTAAAAGCATCGAGCGCCATACGCGTCGGCAGATTAACAGGTGTCCGGGAATCATAACGCTTCCACTGTGAAACCTGTGCCACAGGTTCATTTTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTA  >  NC_000913/1572780‑1573076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: