Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,736,100 T→A 5.9% intergenic (+141/+21) sodB → / ← ydhP superoxide dismutase, Fe/putative MFS transporter, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,736,1000TA 5.9% 527.7 / 15.1 151intergenic (+141/+21)sodB/ydhPsuperoxide dismutase, Fe/putative MFS transporter, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (63/79);  new base A (5/4);  total (68/83)
Fisher's exact test for biased strand distribution p-value = 7.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 153 total aligned reads displayed.

CTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTA  >  NC_000913/1735952‑1736248
                                                                                                                                                    |                                                                                                                                                    
cTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTAt                                                                                                                                                      <  4:565159/149‑1 (MQ=255)
     taataaCTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTc                                                                                                                                                 <  3:163910/149‑1 (MQ=255)
     taataaCTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTc                                                                                                                                                 >  4:930869/1‑149 (MQ=255)
     taataaCTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTc                                                                                                                                                 <  4:560369/149‑1 (MQ=255)
      aataaCTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCt                                                                                                                                                <  2:1321521/149‑1 (MQ=255)
      aataaCTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCt                                                                                                                                                <  3:91618/149‑1 (MQ=255)
        taaCTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGc                                                                                                                                              >  2:451266/1‑149 (MQ=255)
            tGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGttt                                                                                                                                          >  2:264151/1‑149 (MQ=255)
              aTGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGttttt                                                                                                                                        <  2:922148/149‑1 (MQ=255)
                ggCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGt                                                                                                                                      <  2:255007/149‑1 (MQ=255)
                ggCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGt                                                                                                                                      <  3:767563/149‑1 (MQ=255)
                ggCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGt                                                                                                                                      >  2:537262/1‑149 (MQ=255)
                 gCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGtttt                                                                                                                                         <  4:183598/145‑1 (MQ=255)
                 gCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGtt                                                                                                                                     >  4:672499/1‑149 (MQ=255)
                   aaaTGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTc                                                                                                                                                 >  4:480229/1‑135 (MQ=255)
                   aaaTGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTc                                                                                                                                                 <  3:480229/135‑1 (MQ=255)
                   aaaTGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTa                                                                                                                                   <  1:803092/149‑1 (MQ=255)
                    aaTGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTaa                                                                                                                                  <  3:189329/149‑1 (MQ=255)
                     aTGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAAt                                                                                                                                 >  2:921183/1‑149 (MQ=255)
                      tGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAAtt                                                                                                                                >  1:819724/1‑149 (MQ=255)
                       gcagcaTTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTa                                                                                                                               <  1:39151/149‑1 (MQ=255)
                         agcaTTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGc                                                                                                                             >  2:966819/1‑149 (MQ=255)
                         agcaTTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTATGTTTAATTAGc                                                                                                                             >  1:1169990/1‑149 (MQ=255)
                           caTTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGt                                                                                                                                      >  1:106249/1‑138 (MQ=255)
                              tGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTa                                                                                                                        <  1:921183/149‑1 (MQ=255)
                                 cTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCa                                                                                                                     >  1:723387/1‑149 (MQ=255)
                                  tGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCaa                                                                                                                    >  3:427148/1‑149 (MQ=255)
                                   gAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAAc                                                                                                                   <  3:1377022/149‑1 (MQ=255)
                                   gAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAAc                                                                                                                   <  1:335842/149‑1 (MQ=255)
                                   gAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCCTCTGCGTTTTTGTTTAATTAGCTGTTAGCAAc                                                                                                                   >  4:166885/1‑149 (MQ=255)
                                      gcgcTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCt                                                                                                                            >  1:584913/1‑137 (MQ=255)
                                        gcTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCaaa                                                                                                              <  3:452027/149‑1 (MQ=255)
                                        gcTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCaaa                                                                                                              <  4:735468/149‑1 (MQ=255)
                                         cTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGATAACCTTCTGc                                                                                                                                              <  1:987595/116‑1 (MQ=255)
                                         cTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGATAACCTTCTGc                                                                                                                                              >  2:987595/1‑116 (MQ=255)
                                          tACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACt                                                                                                            >  2:416740/1‑149 (MQ=255)
                                            agcgcttCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTACTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGt                                                                                                          <  3:27810/143‑1 (MQ=255)
                                             gCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGtt                                                                                                         >  1:1112002/1‑149 (MQ=255)
                                               ttATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTc                                                                                                       <  4:133331/149‑1 (MQ=255)
                                                 aTCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAg                                                                                                     <  2:781858/149‑1 (MQ=255)
                                                  tCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCTCTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAgg                                                                                                    <  2:563634/149‑1 (MQ=255)
                                                  tCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAgg                                                                                                    <  4:1269927/149‑1 (MQ=255)
                                                   cAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGgt                                                                                                   >  1:53514/1‑149 (MQ=255)
                                                    aGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGgtt                                                                                                  <  3:758967/149‑1 (MQ=255)
                                                    aGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGgtt                                                                                                  <  2:1429565/149‑1 (MQ=255)
                                                      gCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGgttgt                                                                                                <  2:860264/149‑1 (MQ=255)
                                                        cTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGttt                                                                                              <  4:575438/149‑1 (MQ=255)
                                                        cTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGttt                                                                                              >  4:1221993/1‑149 (MQ=255)
                                                           cgcgGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCt                                                                                           <  2:1064649/149‑1 (MQ=255)
                                                           cgcgGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCt                                                                                           >  2:851756/1‑149 (MQ=255)
                                                            gcgGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTg                                                                                          <  3:1290108/149‑1 (MQ=255)
                                                             cgGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTgg                                                                                         <  1:451266/149‑1 (MQ=255)
                                                                atcatcGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTg                                                                                      <  4:890891/149‑1 (MQ=255)
                                                                atcatcGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACACAAACTGTTTCAGGTTGTTTTCt                                                                                           >  2:1140541/1‑144 (MQ=255)
                                                                 tcatcGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCaaa                                                                                                              <  2:1265527/124‑1 (MQ=255)
                                                                 tcatcGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCaaa                                                                                                              >  1:1265527/1‑124 (MQ=255)
                                                                 tcatcGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGa                                                                                     >  4:303217/1‑149 (MQ=255)
                                                                 tcatcGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGa                                                                                     <  1:802519/149‑1 (MQ=255)
                                                                   atcGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGtt                                                                                                                         <  1:1144114/111‑1 (MQ=255)
                                                                          tAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTa                                                                                                                               <  1:1446039/98‑1 (MQ=255)
                                                                          tAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTa                                                                                                                               >  2:1446039/1‑98 (MQ=255)
                                                                            ggTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCa                                                                          <  1:567954/149‑1 (MQ=255)
                                                                            ggTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCa                                                                          <  1:30451/149‑1 (MQ=255)
                                                                              tCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCATGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGc                                                                        >  2:309998/1‑149 (MQ=255)
                                                                              tCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGc                                                                        >  4:1109631/1‑149 (MQ=255)
                                                                                ggATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCt                                                                                           >  3:170056/1‑128 (MQ=255)
                                                                                      ggCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGc                                                                <  1:268248/149‑1 (MQ=255)
                                                                                          cTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGt                                                            >  4:1353859/1‑149 (MQ=255)
                                                                                           tCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTc                                                           <  3:1040037/149‑1 (MQ=255)
                                                                                           tCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTc                                                           >  4:597214/1‑149 (MQ=255)
                                                                                           tCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTc                                                           >  2:1259070/1‑149 (MQ=255)
                                                                                               cgcATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCcgc                                                       >  4:597816/1‑149 (MQ=255)
                                                                                               cgcATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCcgc                                                       <  1:26730/149‑1 (MQ=255)
                                                                                                   tCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACa                                                   >  1:995384/1‑149 (MQ=255)
                                                                                                      ggCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATc                                                >  2:416166/1‑149 (MQ=255)
                                                                                                         aaGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCt                                             >  3:563959/1‑149 (MQ=255)
                                                                                                              aaaTCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCAt                                        <  2:58231/149‑1 (MQ=255)
                                                                                                                 tCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCAc                                     >  3:532942/1‑149 (MQ=255)
                                                                                                                  cGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCAcc                                    <  4:209629/149‑1 (MQ=255)
                                                                                                                   gCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGt                                                                                                          >  2:633581/1‑78 (MQ=255)
                                                                                                                   gCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGt                                                                                                          <  1:633581/78‑1 (MQ=255)
                                                                                                                   gCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCACTCCCATCACCg                                   >  2:720768/1‑149 (MQ=255)
                                                                                                                             aGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCt                         <  1:309998/149‑1 (MQ=255)
                                                                                                                             aGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCt                         <  3:597214/149‑1 (MQ=255)
                                                                                                                             aGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCt                         >  4:285194/1‑149 (MQ=255)
                                                                                                                              gCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTg                        >  3:1366568/1‑149 (MQ=255)
                                                                                                                              gCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTCGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTg                        >  4:1379173/1‑149 (MQ=255)
                                                                                                                               cAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACTCCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGt                       <  1:1395732/149‑1 (MQ=255)
                                                                                                                                aaCTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTa                      <  1:72448/149‑1 (MQ=255)
                                                                                                                                aaCTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTa                      >  3:741659/1‑149 (MQ=255)
                                                                                                                                   tGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATcc                   <  1:827481/149‑1 (MQ=255)
                                                                                                                                   tGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATcc                   <  1:1317750/149‑1 (MQ=255)
                                                                                                                                   aaaaaCGCAGAAGGATAACCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATcc                   >  3:850534/4‑149 (MQ=255)
                                                                                                                                    gTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATccc                  >  4:864144/1‑149 (MQ=255)
                                                                                                                                     tAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATccca                 >  2:329751/1‑149 (MQ=255)
                                                                                                                                       aCGCAGAAGGATAACCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATcccagc               >  3:37302/1‑149 (MQ=255)
                                                                                                                                           aGAAGGATAACCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCt                         <  1:946901/135‑1 (MQ=255)
                                                                                                                                                gTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGaa      >  2:394977/1‑149 (MQ=255)
                                                                                                                                                 ttATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGaaa     >  2:362024/1‑149 (MQ=255)
                                                                                                                                                    tCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTa  <  3:194937/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTA  >  NC_000913/1735952‑1736248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: