Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,090,124 C→G 9.2% intergenic (+78/‑68) hisL → / → hisG his operon leader peptide/ATP phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,090,1240CG 9.2% 487.6 / 32.8 141intergenic (+78/‑68)hisL/hisGhis operon leader peptide/ATP phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (68/60);  new base G (5/8);  total (73/68)
Fisher's exact test for biased strand distribution p-value = 3.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.30e-01

Only 100 of 143 total aligned reads displayed.

AAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTGGCGCGC  >  NC_000913/2089976‑2090272
                                                                                                                                                    |                                                                                                                                                    
aaaCATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATc                                                                                                                                                      <  2:885722/149‑1 (MQ=255)
 aaCATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCa                                                                                                                                                     <  1:1030957/149‑1 (MQ=255)
 aaCATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCa                                                                                                                                                     <  4:654237/149‑1 (MQ=255)
  aCATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCAc                                                                                                                                                    <  3:387961/149‑1 (MQ=255)
    aTTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCt                                                                                                                                                  >  2:591311/1‑149 (MQ=255)
        acaGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCg                                                                                                                                              >  3:369390/1‑149 (MQ=255)
          agagACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGGTGATCTTCCggg                                                                                                                                            <  2:1185685/149‑1 (MQ=255)
          agagACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGGTGATCTTCCggg                                                                                                                                            <  2:148505/149‑1 (MQ=255)
          agagACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGGTGATCTTCCggg                                                                                                                                            <  4:115475/149‑1 (MQ=255)
          agagACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggg                                                                                                                                            >  4:1162706/1‑149 (MQ=255)
           gagaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgggg                                                                                                                                           <  4:180057/149‑1 (MQ=255)
              aCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCt                                                                                                                                        >  2:1167902/1‑149 (MQ=255)
                ttttATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCttt                                                                                                                                      <  2:1132148/149‑1 (MQ=255)
                ttttATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCttt                                                                                                                                      >  1:1187173/1‑149 (MQ=255)
                 tttATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCtttt                                                                                                                                     <  3:1156958/149‑1 (MQ=255)
                  ttATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCttttt                                                                                                                                    <  2:224971/149‑1 (MQ=255)
                     tGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAt                                                                                                                                 <  4:497599/149‑1 (MQ=255)
                        cacGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgc                                                                                                                              >  1:1341830/1‑149 (MQ=255)
                         acGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcg                                                                                                                             >  1:1348307/1‑149 (MQ=255)
                         acGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcg                                                                                                                             >  3:932484/1‑149 (MQ=255)
                             gTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGt                                                                                                                         <  4:1150333/149‑1 (MQ=255)
                              ttCAATTTAAAGACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGtt                                                                                                                        >  3:1279829/1‑149 (MQ=255)
                              ttCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGtt                                                                                                                        <  1:591311/149‑1 (MQ=255)
                              ttCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGtt                                                                                                                        >  3:1031153/1‑149 (MQ=255)
                                   tttAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGAt                                                                                                                     >  1:436588/1‑147 (MQ=255)
                                   tttAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGAt                                                                                                                     <  2:436588/147‑1 (MQ=255)
                                   tttAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATaa                                                                                                                   >  3:1276980/1‑149 (MQ=255)
                                      aaaCACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACgg                                                                                                                >  3:753482/1‑149 (MQ=255)
                                          accaccATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg                                                                                                                                             <  3:428739/116‑1 (MQ=255)
                                            caccatcatcaccatcatCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGa                                                                                                          >  2:1365582/1‑149 (MQ=255)
                                            caccatcatcaccatcatCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCCTTTTTATTGCGCGGTTGATAACGGTTCAGa                                                                                                          >  4:684163/1‑149 (MQ=255)
                                              ccatcatcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACa                                                                                                        >  4:241967/1‑149 (MQ=255)
                                               catcatcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAg                                                                                                       >  3:87706/1‑149 (MQ=255)
                                                   atcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGttt                                                                                                   <  2:1348307/149‑1 (MQ=255)
                                                    tcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggg                                                                                                                                            >  2:538743/1‑107 (MQ=255)
                                                    tcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggg                                                                                                                                            <  1:538743/107‑1 (MQ=255)
                                                        catcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCCGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACATGTTTAAaga                                                                                              <  4:753482/149‑1 (MQ=255)
                                                        catcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAaga                                                                                              <  4:1276980/149‑1 (MQ=255)
                                                            atcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGaa                                                                                          <  1:1365582/149‑1 (MQ=255)
                                                             tcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAagag                                                                                             >  3:1208681/1‑145 (MQ=255)
                                                             tcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAAt                                                                                         >  4:1074780/1‑149 (MQ=255)
                                                             tcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAAt                                                                                         <  2:181635/149‑1 (MQ=255)
                                                               cTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAtt                                                                                                                                >  3:825218/1‑108 (MQ=255)
                                                               cTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAtt                                                                                                                                <  4:825218/108‑1 (MQ=255)
                                                                tGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTc                                                                                                             <  4:282405/126‑1 (MQ=255)
                                                                tGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTc                                                                                                             >  3:282405/1‑126 (MQ=255)
                                                                    tAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACaaaa                                                                                  >  1:226785/1‑149 (MQ=255)
                                                                       tCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATga                                                                               >  2:369924/1‑149 (MQ=255)
                                                                        cTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGCTTCAGACAGGTTTAAAGAGGAATAACAAAATgaa                                                                              <  2:351188/149‑2 (MQ=255)
                                                                         tttCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCtttttt                                                                                                                                   >  4:1366613/1‑95 (MQ=255)
                                                                           tCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacaga                                                                           <  2:130998/149‑1 (MQ=255)
                                                                                      gtgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGtt                                                                <  2:866898/149‑1 (MQ=255)
                                                                                         cTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgaca                                                                             <  3:81231/133‑1 (MQ=255)
                                                                                         cTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgaca                                                                             >  4:81231/1‑133 (MQ=255)
                                                                                         cTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTAc                                                             >  3:430875/1‑149 (MQ=255)
                                                                                          tGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGa                                                                                                                      >  1:980756/1‑91 (MQ=255)
                                                                                          tGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGa                                                                                                                      <  2:980756/91‑1 (MQ=255)
                                                                                            gAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTa                                                                                                                                  >  2:946759/1‑77 (MQ=255)
                                                                                            gAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTa                                                                                                                                  <  1:946759/77‑1 (MQ=255)
                                                                                            gAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGacaa                                                                        <  3:1331175/135‑1 (MQ=255)
                                                                                            gAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCa                                                          >  4:1066300/1‑149 (MQ=255)
                                                                                               gACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAg                                                       >  4:868816/1‑149 (MQ=255)
                                                                                                  aTTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTa                                                    >  2:1400856/1‑149 (MQ=255)
                                                                                                  aTTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTa                                                    >  3:798189/1‑149 (MQ=255)
                                                                                                  aTTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTa                                                    >  2:453867/1‑149 (MQ=255)
                                                                                                  aTTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTa                                                    >  3:1300915/1‑149 (MQ=255)
                                                                                                   ttCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTAt                                                   >  1:825016/1‑149 (MQ=255)
                                                                                                   ttCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTAt                                                   >  1:107153/1‑149 (MQ=255)
                                                                                                      aGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGtt                                                                                                              <  2:1143838/87‑1 (MQ=255)
                                                                                                      aGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGtt                                                                                                              >  1:1143838/1‑87 (MQ=255)
                                                                                                       gATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAg                                               >  1:1112887/1‑149 (MQ=255)
                                                                                                       gATCCTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTaaa                                                                                                >  4:1292626/1‑100 (MQ=255)
                                                                                                         tCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGtt                                                                                                              >  2:1085674/1‑84 (MQ=255)
                                                                                                         tCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGtt                                                                                                              <  1:1085674/84‑1 (MQ=255)
                                                                                                           ttCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGtt                                                                                                    >  3:56162/1‑92 (MQ=255)
                                                                                                           ttCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGtt                                                                                                    <  4:56162/92‑1 (MQ=255)
                                                                                                                gtggtgCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGc                                      <  4:87706/149‑1 (MQ=255)
                                                                                                                     gCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGttt                                 <  3:1162706/149‑1 (MQ=255)
                                                                                                                      cATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTa                                >  1:66356/1‑149 (MQ=255)
                                                                                                                       aTGAACGCATGGGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTaa                               >  4:1386641/1‑149 (MQ=255)
                                                                                                                        tGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgggg                                                                                                                                           <  4:442851/40‑1 (MQ=255)
                                                                                                                         gAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGt                             >  3:459303/1‑149 (MQ=255)
                                                                                                                           aCGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGtga                           <  3:747535/149‑1 (MQ=255)
                                                                                                                           aCGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGtga                           <  4:459303/149‑1 (MQ=255)
                                                                                                                            cGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGtgat                          <  3:241967/149‑1 (MQ=255)
                                                                                                                            cGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGtgat                          <  3:1074780/149‑1 (MQ=255)
                                                                                                                               aTGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGAc                       >  2:285138/1‑149 (MQ=255)
                                                                                                                                     aaGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCAcgc                 <  4:430875/149‑1 (MQ=255)
                                                                                                                                         ccccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAagag                                                                                             <  2:1340700/69‑1 (MQ=255)
                                                                                                                                          cccGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTAt                                                   >  3:623804/1‑110 (MQ=255)
                                                                                                                                          cccGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTAt                                                   <  4:623804/110‑1 (MQ=255)
                                                                                                                                          cccGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTaa                               >  2:196265/1‑130 (MQ=255)
                                                                                                                                          cccGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTaa                               <  1:196265/130‑1 (MQ=255)
                                                                                                                                          cccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGcga                           >  3:45427/1‑134 (MQ=255)
                                                                                                                                          cccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAAtt            <  3:1386641/149‑1 (MQ=255)
                                                                                                                                           ccGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTg           >  2:1169828/1‑149 (MQ=255)
                                                                                                                                              gAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTg        <  1:1237742/149‑1 (MQ=255)
                                                                                                                                                  aTCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTaa                               >  2:1108570/1‑122 (MQ=255)
                                                                                                                                                  aTCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAAtt            >  2:889415/1‑141 (MQ=255)
                                                                                                                                                    cACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTGgcgcgc  <  3:503726/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTGGCGCGC  >  NC_000913/2089976‑2090272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: