Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,165,626 T→C 6.4% intergenic (+105/‑42) yegP → / → yegQ UPF0339 family protein/putative peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,165,6260TC 6.4% 496.7 / 14.0 153intergenic (+105/‑42)yegP/yegQUPF0339 family protein/putative peptidase
Reads supporting (aligned to +/- strand):  ref base T (65/78);  new base C (5/5);  total (70/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 163 total aligned reads displayed.

AAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCT  >  NC_000913/2165481‑2165774
                                                                                                                                                 |                                                                                                                                                    
aaGCCAATGGCACAAGCCAGATGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCGGAGCCCCCCACAGCGCTACAATGCCCGCCCTTACAGTGGGGGCACTCCCCTAACCGCTTCATCAg                                                                                                                                                   <  3:1216598/149‑1 (MQ=255)
aaGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAg                                                                                                                                                   <  4:456918/149‑1 (MQ=255)
  gCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGt                                                                                                                                                 >  1:198455/1‑149 (MQ=255)
   ccAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTg                                                                                                                                                >  1:489805/1‑149 (MQ=255)
    cAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGa                                                                                                                                               >  3:611879/1‑149 (MQ=255)
       tGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGc                                                                                                                                            <  2:812935/149‑1 (MQ=255)
          cacaAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGa                                                                                                                                         <  3:1030985/149‑1 (MQ=255)
           acaAGCCAGACGGTGAAAGACACTACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTCCAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGAt                                                                                                                                        <  1:1268649/149‑1 (MQ=255)
           acaAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGAt                                                                                                                                        >  1:831764/1‑149 (MQ=255)
           acaAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGAt                                                                                                                                        >  3:436938/1‑149 (MQ=255)
           acaAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAACGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGAt                                                                                                                                        >  1:527785/1‑149 (MQ=255)
            caAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATc                                                                                                                                       <  1:1231336/149‑1 (MQ=255)
             aaGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCt                                                                                                                                      >  1:38020/1‑149 (MQ=255)
             aaGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCt                                                                                                                                      >  1:1025060/1‑149 (MQ=255)
               gCCAGACGGTGAAAGACAATCCGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGa                                                                                                                                    <  1:130514/149‑1 (MQ=255)
               gCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGaa                                                                                                                                              <  1:1027851/139‑1 (MQ=255)
               gCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGa                                                                                                                                    <  1:523701/149‑1 (MQ=255)
                ccAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGAc                                                                                                                                   <  4:1057989/149‑1 (MQ=255)
                ccAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGAc                                                                                                                                   >  3:732211/1‑149 (MQ=255)
                 cAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGAcc                                                                                                                                  <  4:627700/149‑1 (MQ=255)
                 cAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGAcc                                                                                                                                  <  4:1029430/149‑1 (MQ=255)
                  aGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCACCTGATGAAGCg                                                                                                                                           >  2:824943/1‑139 (MQ=255)
                  aGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCACCTGATGAAGCg                                                                                                                                           <  1:824943/139‑1 (MQ=255)
                        tGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGtcatc                                                                                                                           >  2:748639/1‑149 (MQ=255)
                         gAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGtcatca                                                                                                                          >  3:939270/1‑149 (MQ=255)
                            aGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGaa                                                                                                                       >  2:913361/1‑149 (MQ=255)
                             gACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAAc                                                                                                                      >  2:1073121/1‑149 (MQ=255)
                             gACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCACCTGATGAAGCggtt                                                                                                                                        >  2:940703/1‑129 (MQ=255)
                             gACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCACCTGATGAAGCggtt                                                                                                                                        <  1:940703/131‑3 (MQ=255)
                              aCAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGTCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACg                                                                                                                     >  2:1150167/1‑149 (MQ=255)
                               cAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACga                                                                                                                    <  4:611879/149‑1 (MQ=255)
                                 aTACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACgaga                                                                                                                  <  1:870060/149‑1 (MQ=255)
                                  tACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCg                                                                                                                                           <  4:1453537/123‑1 (MQ=255)
                                  tACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCg                                                                                                                                           >  3:1453537/1‑123 (MQ=255)
                                  tACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACgagag                                                                                                                 <  2:1167360/149‑1 (MQ=255)
                                  tACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACgagag                                                                                                                 <  2:1296967/149‑1 (MQ=255)
                                   aCGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACgagaga                                                                                                                <  1:1083226/149‑1 (MQ=255)
                                    cGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGaa                                                                                                               <  2:1275690/149‑1 (MQ=255)
                                     gTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACTGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAAt                                                                                                              <  4:929933/149‑1 (MQ=255)
                                     gTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGtcatca                                                                                                                          >  4:1392221/1‑137 (MQ=255)
                                     gTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAAt                                                                                                              >  4:1251765/1‑149 (MQ=255)
                                     gTAATTACGATGCCGGGCGTGGGTTGTTGGCGTACGGCGCTTTACCAGAGCCACCCACATCGCTACAATACCCACCCTTAAAGCCGGGGGACTCCCCTATCCCCTTCATCAGGTGAAGCGGATCTTGCCTCTCATCAGAACGAGAGAAt                                                                                                              <  4:1152577/149‑1 (MQ=255)
                                        aTTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTAt                                                                                                           <  2:218133/149‑1 (MQ=255)
                                         ttACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATg                                                                                                          <  2:669884/149‑1 (MQ=255)
                                         ttACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATg                                                                                                          <  1:913361/149‑1 (MQ=255)
                                          tACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGt                                                                                                         <  4:436938/149‑1 (MQ=255)
                                           aCGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGtt                                                                                                        <  3:1398613/149‑1 (MQ=255)
                                           aCGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGtt                                                                                                        <  1:567888/149‑1 (MQ=255)
                                             gATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTa                                                                                                      >  1:751370/1‑149 (MQ=255)
                                                       gTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGaa                                                                                                                       >  2:828716/1‑122 (MQ=255)
                                                        tGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCt                                                                                                                                      <  3:335989/106‑1 (MQ=255)
                                                        tGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCt                                                                                                                                      >  4:335989/1‑106 (MQ=255)
                                                         gggTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTcc                                                                                          <  3:465900/149‑1 (MQ=255)
                                                            ttgttgGCGTCCGGCGCTTTACCAGAGCACCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGaa                                                                                                               <  3:1075491/125‑1 (MQ=255)
                                                            ttgttgGCGTCCGGCGCTTTACCAGAGCACCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGaa                                                                                                               >  4:1075491/1‑125 (MQ=255)
                                                                             tttACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACga                                                                                                                    >  1:627556/1‑103 (MQ=255)
                                                                             tttACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACga                                                                                                                    <  2:627556/103‑1 (MQ=255)
                                                                                   agagCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAat                                                                >  4:1370053/1‑149 (MQ=255)
                                                                                    gagCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAata                                                               >  1:731617/1‑149 (MQ=255)
                                                                                     agCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAatat                                                              >  3:125941/1‑149 (MQ=255)
                                                                                       ccccccACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGc                                                            <  3:729693/149‑1 (MQ=255)
                                                                                         ccccACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGt                                                          >  2:508993/1‑149 (MQ=255)
                                                                                          cccACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGtt                                                         >  1:1433386/1‑149 (MQ=255)
                                                                                          cccACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGtt                                                         >  1:415539/1‑149 (MQ=255)
                                                                                           ccACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTaaa                                                                                                    <  3:296449/105‑1 (MQ=255)
                                                                                           ccACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTc                                                                                           <  1:1231647/114‑1 (MQ=255)
                                                                                           ccACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTa                                                        <  1:1014508/149‑1 (MQ=255)
                                                                                                  gcTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTc                                                 <  3:1067982/149‑1 (MQ=255)
                                                                                                    tACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGc                                               >  3:1300270/1‑149 (MQ=255)
                                                                                                      cAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCtt                                             >  3:11847/1‑149 (MQ=255)
                                                                                                      cAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCtt                                             <  1:1465392/149‑1 (MQ=255)
                                                                                                       aaTGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTa                                            <  1:789814/149‑1 (MQ=255)
                                                                                                            ccgcccTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGgcg                                       >  1:860908/1‑149 (MQ=255)
                                                                                                                ccTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGTGGATCTGACCTGTCATCAGAACGAGAGAATTAAGATTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAACATATGCGTTACGCTTTCGCTTATGGCGCAGa                                   >  3:901632/1‑149 (MQ=255)
                                                                                                                ccTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGa                                   <  2:714560/149‑1 (MQ=255)
                                                                                                                 cTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGAt                                  <  4:527501/149‑1 (MQ=255)
                                                                                                                  ttAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATg                                 >  1:1251625/1‑149 (MQ=255)
                                                                                                                   tAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGCAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGc                                >  3:1377376/1‑149 (MQ=255)
                                                                                                                      aGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGt                             >  3:1207461/1‑149 (MQ=255)
                                                                                                                      aGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATAAGCGTTACGCTTTCGCTTATGGCGCAGATGCTGt                             <  3:924282/149‑1 (MQ=255)
                                                                                                                        tGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGttt                           <  2:1250303/149‑1 (MQ=255)
                                                                                                                            ggCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATg                                          <  3:254536/130‑1 (MQ=255)
                                                                                                                            ggCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATg                                          >  4:254536/1‑130 (MQ=255)
                                                                                                                            ggCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGc                       <  2:198455/149‑1 (MQ=255)
                                                                                                                                 tCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGc                                                            <  2:849822/107‑1 (MQ=255)
                                                                                                                                 tCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGcc                  >  1:590636/1‑149 (MQ=255)
                                                                                                                                 tCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGcc                  >  4:241685/1‑149 (MQ=255)
                                                                                                                                   cccTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGAt                                  <  3:1336460/131‑1 (MQ=255)
                                                                                                                                   cccTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGAt                                  >  4:1336460/1‑131 (MQ=255)
                                                                                                                                   cccTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGtt                            >  3:545303/1‑137 (MQ=255)
                                                                                                                                        tccGCTTCACCTGATGAAGCGGATCTGACCTGCCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCcgcg           >  1:828291/2‑149 (MQ=255)
                                                                                                                                         ccGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGt          >  4:3254/1‑149 (MQ=255)
                                                                                                                                         ccGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGt          >  1:1016808/1‑149 (MQ=255)
                                                                                                                                           gCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGttt                                                                                                       <  4:1366489/54‑1 (MQ=255)
                                                                                                                                               catcaGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTcc    <  3:135808/149‑1 (MQ=255)
                                                                                                                                                atcaGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTccc   <  4:732211/149‑1 (MQ=255)
                                                                                                                                                 tcaGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTa                                            <  3:615681/107‑1 (MQ=255)
                                                                                                                                                 tcaGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCt  >  4:611544/1‑149 (MQ=255)
                                                                                                                                                 tcaGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCt  >  3:1270203/1‑149 (MQ=255)
                                                                                                                                                 tcaGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGACAGCCGCGTTATTCCCt  >  4:734321/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
AAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCT  >  NC_000913/2165481‑2165774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: