Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,336,763 A→C 5.3% intergenic (‑119/+30) yfaA ← / ← gyrA DUF2138 family protein, putative host defense protein/DNA gyrase (type II topoisomerase), subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,336,7630AC 5.3% 469.6 / 15.8 133intergenic (‑119/+30)yfaA/gyrADUF2138 family protein, putative host defense protein/DNA gyrase (type II topoisomerase), subunit A
Reads supporting (aligned to +/- strand):  ref base A (65/61);  new base C (4/3);  total (69/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01

Only 100 of 137 total aligned reads displayed.

CCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTT  >  NC_000913/2336615‑2336911
                                                                                                                                                    |                                                                                                                                                    
ccAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGgaga                                                                                                                                                      >  4:576058/1‑149 (MQ=255)
  aGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATa                                                                                                                                                    >  3:1213185/1‑149 (MQ=255)
   gCCTTTCGCCTGTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAg                                                                                                                                                   <  1:655718/149‑1 (MQ=255)
    ccTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGc                                                                                                                                                  <  3:885436/149‑1 (MQ=255)
    ccTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGc                                                                                                                                                  >  2:117592/1‑149 (MQ=255)
          gCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCtt                                                                                                                                            >  3:494380/1‑149 (MQ=255)
            cTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGCTATCTCCCttgt                                                                                                                                          <  2:1324431/149‑3 (MQ=255)
            cTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCtttt                                                                                                                                          <  4:888527/149‑1 (MQ=255)
            cTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCtttt                                                                                                                                          >  3:888527/1‑149 (MQ=255)
            cTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCtttt                                                                                                                                          <  2:621636/149‑1 (MQ=255)
            cTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCtttt                                                                                                                                          <  2:814980/149‑1 (MQ=255)
             tttttttCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTg                                                                                                                                         >  3:1382735/1‑149 (MQ=255)
             tttttttCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTg                                                                                                                                         <  3:1346148/149‑1 (MQ=255)
             tttttttCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTg                                                                                                                                         >  3:677559/1‑149 (MQ=255)
              ttttttCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTgg                                                                                                                                        <  1:1423615/149‑1 (MQ=255)
              ttttttCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTgg                                                                                                                                        <  2:1454272/149‑1 (MQ=255)
                      ccacTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATgaaga                                                                                                                                >  1:375796/1‑149 (MQ=255)
                       cacTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATgaagaa                                                                                                                               >  2:1067889/1‑149 (MQ=255)
                       cacTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATgaagaa                                                                                                                               <  1:862082/149‑1 (MQ=255)
                       cacTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATgaagaa                                                                                                                               >  1:644836/1‑149 (MQ=255)
                       cacTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATgaagaa                                                                                                                               >  4:522649/1‑149 (MQ=255)
                       cacTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATgaagaa                                                                                                                               <  1:1072527/149‑1 (MQ=255)
                        acTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGaagaag                                                                                                                              <  2:1178938/149‑1 (MQ=255)
                         cTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGt                                                                                                                             <  3:1239303/149‑1 (MQ=255)
                         cTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGt                                                                                                                             <  1:1368221/149‑1 (MQ=255)
                         cTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGt                                                                                                                             >  3:33951/1‑149 (MQ=255)
                          tCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTa                                                                                                                            >  2:1075505/1‑149 (MQ=255)
                            aTTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGCTATCTCCCttgtt                                                                                                                                         <  1:818462/134‑4 (MQ=255)
                            aTTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGCTATCTCCCttgtt                                                                                                                                         >  2:818462/1‑131 (MQ=255)
                             ttGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTaaaa                                                                                                                         <  3:576058/149‑1 (MQ=255)
                             ttGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTaaaa                                                                                                                         >  4:82283/1‑149 (MQ=255)
                                 cacaTTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAattat                                                                                                                     <  4:1382735/149‑1 (MQ=255)
                                 cacaTGCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAattat                                                                                                                     <  1:472153/149‑1 (MQ=255)
                                  acaTTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAattatt                                                                                                                    <  3:239261/149‑1 (MQ=255)
                                  acaTTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAattatt                                                                                                                    <  1:1080625/149‑1 (MQ=255)
                                     ttCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTAttctt                                                                                                                 <  4:33951/149‑1 (MQ=255)
                                      tCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTAttcttc                                                                                                                <  2:299526/149‑1 (MQ=255)
                                      tCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTAttcttc                                                                                                                <  1:1159091/149‑1 (MQ=255)
                                         ttGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTAttcttcttc                                                                                                             >  4:652428/1‑149 (MQ=255)
                                         ttGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTAttcttcttc                                                                                                             <  3:652428/149‑1 (MQ=255)
                                          tgtgTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATtcttcttct                                                                                                            <  4:691527/149‑1 (MQ=255)
                                            tgtATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTgg                                                                                                          <  3:269491/149‑1 (MQ=255)
                                            tgtATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTgg                                                                                                          <  4:972419/149‑1 (MQ=255)
                                             gtATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGc                                                                                                         >  4:1220332/1‑149 (MQ=255)
                                                    cagccaTTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTcgtcgt                                                                                                  <  4:1266983/149‑1 (MQ=255)
                                                       ccaTTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCaa                                                                                               >  1:1070696/1‑149 (MQ=255)
                                                        caTTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAGGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTCAAATTATTCTTCTTCTGGCTCGTCGTCAAc                                                                                              >  1:957837/1‑149 (MQ=255)
                                                         aTTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTGATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACg                                                                                             >  4:1379312/1‑149 (MQ=255)
                                                                  gggCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTcc                                                                                    >  4:347294/1‑149 (MQ=255)
                                                                   ggCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCg                                                                                   <  1:233020/149‑1 (MQ=255)
                                                                    tcacaGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGCGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCgg                                                                                  >  4:945366/2‑149 (MQ=255)
                                                                    gCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCgg                                                                                  >  1:62235/1‑149 (MQ=255)
                                                                      acaGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAg                                                                                >  3:449882/1‑149 (MQ=255)
                                                                        aGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTCTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGCAGCg                                                                              >  4:1172109/1‑149 (MQ=255)
                                                                         gCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGa                                                                             >  1:244040/1‑149 (MQ=255)
                                                                         gCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGa                                                                             >  3:628506/1‑149 (MQ=255)
                                                                            aaaCTTTACCGTGCCCTAATACGACAAAAGCCCAGTCTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGAttt                                                                          >  2:1215600/1‑149 (MQ=255)
                                                                             aaCTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTc                                                                         <  4:748410/149‑1 (MQ=255)
                                                                              aCTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCa                                                                        >  2:1356931/1‑149 (MQ=255)
                                                                                tttACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATc                                                                      <  3:1053360/149‑1 (MQ=255)
                                                                                tttACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATc                                                                      <  2:1097996/149‑1 (MQ=255)
                                                                                          acTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTcc                                                            <  4:182642/148‑1 (MQ=255)
                                                                                           cTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCcg                                                           >  2:1150951/1‑149 (MQ=255)
                                                                                             aaTACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCcgcg                                                         >  2:925344/1‑149 (MQ=255)
                                                                                               tACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGc                                                       >  1:492847/1‑149 (MQ=255)
                                                                                               tACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGc                                                       >  3:438600/1‑149 (MQ=255)
                                                                                                  gACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACt                                                    >  4:41090/1‑149 (MQ=255)
                                                                                                  gACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACt                                                    >  2:565956/1‑149 (MQ=255)
                                                                                                   aCAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTg                                                   >  2:558730/1‑149 (MQ=255)
                                                                                                   aCAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTg                                                   >  3:383404/1‑149 (MQ=255)
                                                                                                     aaaaGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGcc                                                 >  2:950419/1‑149 (MQ=255)
                                                                                                      aaaGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCg                                                >  3:624701/1‑149 (MQ=255)
                                                                                                       aaGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGt                                               >  1:43901/1‑149 (MQ=255)
                                                                                                        aGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTc                                              <  1:558730/149‑1 (MQ=255)
                                                                                                          cccAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGa                                            <  2:62235/149‑1 (MQ=255)
                                                                                                           ccAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCTCTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGAt                                           >  4:1234532/1‑149 (MQ=255)
                                                                                                            cAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATg                                          <  1:1223523/149‑1 (MQ=255)
                                                                                                            cAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATg                                          >  1:334230/1‑149 (MQ=255)
                                                                                                                      aGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGAt                                >  4:1212895/1‑149 (MQ=255)
                                                                                                                        ccTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCt                              <  4:438600/149‑1 (MQ=255)
                                                                                                                         cTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCtt                             >  1:1295943/1‑149 (MQ=255)
                                                                                                                          tGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTc                            <  2:244040/149‑1 (MQ=255)
                                                                                                                           ggACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTcc                           <  3:522649/149‑1 (MQ=255)
                                                                                                                             aCTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTAttcttct                                                                                                               <  3:1292162/63‑1 (MQ=255)
                                                                                                                             aCTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTAttcttct                                                                                                               >  4:1292162/1‑63 (MQ=255)
                                                                                                                               ttttCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGt                       >  4:1297967/1‑149 (MQ=255)
                                                                                                                               ttttCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGt                       >  2:993195/1‑149 (MQ=255)
                                                                                                                               ttttCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGt                       >  1:154348/1‑149 (MQ=255)
                                                                                                                                   caattcaaACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAAc                   <  4:727516/149‑1 (MQ=255)
                                                                                                                                   caattcaaACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAAc                   <  3:690458/149‑1 (MQ=255)
                                                                                                                                    aattcaaACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAAcc                  >  1:139242/1‑149 (MQ=255)
                                                                                                                                         aaaCAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCa                                                                        <  4:1348941/90‑1 (MQ=255)
                                                                                                                                         aaaCAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTc             >  4:446411/1‑149 (MQ=255)
                                                                                                                                          caaaaGGGAGCTATCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGa                                                                                 <  4:1007561/77‑1 (MQ=255)
                                                                                                                                          aaCAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCa            <  1:1195482/149‑1 (MQ=255)
                                                                                                                                           aaaaGGGAGCTATCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAg           >  2:1030162/3‑149 (MQ=255)
                                                                                                                                           aaaaGGGAGCTATCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAg           >  1:554677/3‑149 (MQ=255)
                                                                                                                                                ggAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCCACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAaca      <  3:1379312/149‑1 (MQ=255)
                                                                                                                                                 gagaTAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAacac     >  2:605936/1‑149 (MQ=255)
                                                                                                                                                    aTAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGtt  <  3:10668/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTCCCTTTTGGCATGAAGAAGTAAAATTATTCTTCTTCTGGCTCGTCGTCAACGTCCACTTCCGGAGCGATTTCATCGTCCCCTTCCGCGGCACTGCCGTCGATGGTATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTT  >  NC_000913/2336615‑2336911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: