Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,375,483 A→T 5.4% L160L (CTT→CTA) ‡ menC ← O‑succinylbenzoyl‑CoA synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,375,4830AT 5.4% 590.6 / 17.1 166L160L (CTT→CTA) ‡menCO‑succinylbenzoyl‑CoA synthase
Reads supporting (aligned to +/- strand):  ref base A (71/86);  new base T (6/3);  total (77/89)
Fisher's exact test for biased strand distribution p-value = 3.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

Only 100 of 167 total aligned reads displayed.

CAATGCCGGTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCGGATCGCCAT  >  NC_000913/2375338‑2375631
                                                                                                                                                 |                                                                                                                                                    
caatgCCGGTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTACGTCAAGa                                                                                                                                                   <  3:813301/149‑1 (MQ=255)
 aatgCCGGTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGAc                                                                                                                                                  <  1:515776/149‑1 (MQ=255)
   tgCCGGTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGc                                                                                                                                                >  1:764650/1‑149 (MQ=255)
      cGGTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCaaa                                                                                                                                             >  4:1045682/1‑149 (MQ=255)
      cGGTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCaaa                                                                                                                                             <  2:764650/149‑1 (MQ=255)
       ggTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAAt                                                                                                                                            >  2:1151233/1‑149 (MQ=255)
        gTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATg                                                                                                                                           <  4:760748/149‑1 (MQ=255)
          ttCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCa                                                                                                                                         <  1:303208/149‑1 (MQ=255)
              cGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGACGCGATTTGCGTCAAGACGCAAATGCAGATc                                                                                                                                     <  4:1328108/149‑1 (MQ=255)
               gggCAAACCCTCGCGAATAATCGCGGGTTTTGCACGGCACTTCGACAAACCCGATGTGGCCGCGATAATCAGGGTGAACGTATTTGGCAAACTGCTGTCCTTACAGCGGTGTCAAGGCGCGATTTGCGGCAAGACGCAAAGGCAGAtgc                                                                                                                                    >  4:931889/1‑147 (MQ=255)
                    aaCGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAAt                                                                                                                               >  3:912269/1‑149 (MQ=255)
                     aCGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAAtt                                                                                                                              >  1:223545/1‑149 (MQ=255)
                       gCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGc                                                                                                                            <  3:919363/149‑1 (MQ=255)
                         tCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCt                                                                                                                          >  3:891984/1‑149 (MQ=255)
                          cgcgAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTc                                                                                                                         <  4:758772/149‑1 (MQ=255)
                           gcgAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGc                                                                                                                            >  1:955256/1‑145 (MQ=255)
                           gcgAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTcc                                                                                                                        <  4:370144/149‑1 (MQ=255)
                           gcgAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTcc                                                                                                                        >  3:1093164/1‑149 (MQ=255)
                            cgAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAGATGCAGATCCGGAACTGCCTCCa                                                                                                                       >  4:1329419/1‑149 (MQ=255)
                            cgAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCa                                                                                                                       <  4:1015021/149‑1 (MQ=255)
                            cgAATCATCGCGGGTTGTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCa                                                                                                                       <  1:818716/149‑1 (MQ=255)
                                    cgcgGGTTTTGCACGGCTCTTCGAGAAACGCGATGTGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGa                                                                                                               <  1:643438/149‑1 (MQ=255)
                                    cgcgGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGa                                                                                                               <  3:601264/149‑1 (MQ=255)
                                     gcgGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGAt                                                                                                              >  4:269464/1‑149 (MQ=255)
                                     gcgGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGAt                                                                                                              >  3:1159457/1‑149 (MQ=255)
                                      cgGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGAtt                                                                                                             >  2:557684/1‑149 (MQ=255)
                                       gggTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTc                                                                                                            <  3:365613/149‑1 (MQ=255)
                                                aCGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATg                                                                                                   <  4:1093164/149‑1 (MQ=255)
                                                     tctTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTc                                                                                              >  3:1208451/1‑149 (MQ=255)
                                                       ttCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGGGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTcgc                                                                                            >  1:963463/1‑149 (MQ=255)
                                                       ttCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTcgc                                                                                            >  2:362765/1‑149 (MQ=255)
                                                          gagaAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCa                                                                                         >  1:189917/1‑149 (MQ=255)
                                                          gagaAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCa                                                                                         >  3:385845/1‑149 (MQ=255)
                                                               aCGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGCCAAACTGCTAACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACTATGCCGTCGCGCACCCCt                                                                                    <  4:478896/149‑1 (MQ=255)
                                                                cgcgATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCtt                                                                                   >  3:132908/1‑149 (MQ=255)
                                                                   gATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGt                                                                                <  3:1012439/149‑1 (MQ=255)
                                                                        ggTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAAt                                                                           <  4:235339/149‑1 (MQ=255)
                                                                        ggTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAAt                                                                           <  2:1118410/149‑1 (MQ=255)
                                                                           cgcgATAATCCGGGTTAACGTATTTGGCAAACAGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATccc                                                                        <  4:1190016/149‑1 (MQ=255)
                                                                            gcgATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCa                                                                       <  3:269464/149‑1 (MQ=255)
                                                                              gATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACt                                                                     <  2:815214/149‑1 (MQ=255)
                                                                                  aTCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGa                                                                 >  4:62497/1‑149 (MQ=255)
                                                                                    ccGGGTTAACGTATTTGGCAAACTCCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATGCCACTTTGAcc                                                               <  3:962474/149‑1 (MQ=255)
                                                                                       ggTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTc                                                            <  3:242975/149‑1 (MQ=255)
                                                                                         ttAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGc                                                          <  1:329539/149‑1 (MQ=255)
                                                                                            aCGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCAc                                                       <  4:621444/149‑1 (MQ=255)
                                                                                            aCGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCAc                                                       >  3:1267245/1‑149 (MQ=255)
                                                                                            aCGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCAc                                                       <  1:277555/149‑1 (MQ=255)
                                                                                              gTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACtt                                                     >  2:787931/1‑149 (MQ=255)
                                                                                              gTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACtt                                                     <  2:1359786/149‑1 (MQ=255)
                                                                                               tATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCt                                                              >  3:1381857/1‑139 (MQ=255)
                                                                                               tATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCt                                                              <  4:1381857/139‑1 (MQ=255)
                                                                                               tATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACttt                                                    >  2:444459/1‑149 (MQ=255)
                                                                                                    ggCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGc                                               >  4:13444/1‑149 (MQ=255)
                                                                                                      cAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCt                                             <  1:1009876/149‑1 (MQ=255)
                                                                                                      cAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCt                                             <  2:963463/149‑1 (MQ=255)
                                                                                                       aaaCTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTg                                            >  4:91737/1‑149 (MQ=255)
                                                                                                        aaCTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTc                                                                                                                         <  3:630512/71‑1 (MQ=255)
                                                                                                           tgctgACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCat                                        >  4:143212/1‑149 (MQ=255)
                                                                                                            gctgACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCa                                                                       >  2:1250410/1‑117 (MQ=255)
                                                                                                              tgACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAAc                                                                                                                  <  4:503049/72‑1 (MQ=255)
                                                                                                              tgACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAAc                                                                                                                  >  3:503049/1‑72 (MQ=255)
                                                                                                                aCCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTg                                   >  1:1179504/1‑149 (MQ=255)
                                                                                                                  cTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCa                                 >  2:1219290/1‑149 (MQ=255)
                                                                                                                   tttCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCaa                                >  3:1179630/1‑149 (MQ=255)
                                                                                                                         cGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGa                          <  1:1136956/149‑1 (MQ=255)
                                                                                                                         cGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGa                          <  2:512303/149‑1 (MQ=255)
                                                                                                                          ggTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAg                         <  1:1219290/149‑1 (MQ=255)
                                                                                                                          ggTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAg                         <  1:1096866/149‑1 (MQ=255)
                                                                                                                          ggTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAg                         <  1:358105/149‑1 (MQ=255)
                                                                                                                          ggTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAg                         <  2:1308110/149‑1 (MQ=255)
                                                                                                                             gtCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGAt                      <  1:915712/149‑1 (MQ=255)
                                                                                                                              tCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATc                     >  1:1379023/1‑149 (MQ=255)
                                                                                                                               ccAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCa                    <  2:223545/149‑1 (MQ=255)
                                                                                                                                 aGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGa                  <  3:1381882/149‑1 (MQ=255)
                                                                                                                                 aGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGa                  >  3:1070330/1‑149 (MQ=255)
                                                                                                                                 aGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGa                  >  2:14754/1‑149 (MQ=255)
                                                                                                                                 aGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGa                  >  4:1455386/1‑149 (MQ=255)
                                                                                                                                 aGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGAACTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGa                  >  1:437747/1‑149 (MQ=255)
                                                                                                                                  ggCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGAt                 <  3:401857/149‑1 (MQ=255)
                                                                                                                                     gcgATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGt              <  3:234378/149‑1 (MQ=255)
                                                                                                                                      gcaTTTGCGTCTTGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTc             >  1:4620/3‑149 (MQ=255)
                                                                                                                                        aTTTGCGTCTTGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACGGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGAt                      >  4:1193453/1‑138 (MQ=255)
                                                                                                                                        aTTTGCGTCTTGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCg           >  1:570249/1‑149 (MQ=255)
                                                                                                                                        aTTTGCGTCTTGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCg           >  4:661987/1‑149 (MQ=255)
                                                                                                                                        aTTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCt                                             >  2:678983/1‑115 (MQ=255)
                                                                                                                                        aTTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCg           >  2:923414/1‑149 (MQ=255)
                                                                                                                                        aTTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCg           <  1:787931/149‑1 (MQ=255)
                                                                                                                                         tttGCGTCTTGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATc                     <  2:1448108/138‑1 (MQ=255)
                                                                                                                                         tttGCGTCTTGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATc                     >  1:1448108/1‑138 (MQ=255)
                                                                                                                                         tttGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCgg          >  4:1065590/1‑149 (MQ=255)
                                                                                                                                           tGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCGGAt        <  2:1052371/149‑1 (MQ=255)
                                                                                                                                            gCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCGGATc       <  3:180831/149‑1 (MQ=255)
                                                                                                                                              gTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCGGATCGc     <  2:970067/149‑1 (MQ=255)
                                                                                                                                              gTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCGGATCGc     <  3:1065549/149‑1 (MQ=255)
                                                                                                                                              gTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCGGATCGc     >  4:540850/1‑149 (MQ=255)
                                                                                                                                               tCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTTAGGATCAGATCGTCCGGATCGcc    >  4:337272/1‑149 (MQ=255)
                                                                                                                                               tCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCGGATCGcc    >  1:1346586/1‑149 (MQ=255)
                                                                                                                                               tCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCGGATCGcc    <  1:1377466/149‑1 (MQ=255)
                                                                                                                                                 aaGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCGGATCGCCAt  >  3:1302962/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CAATGCCGGTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCGGATCGCCAT  >  NC_000913/2375338‑2375631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: