Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,425,968 T→G 6.1% intergenic (‑52/+38) hisQ ← / ← hisJ histidine ABC transporter permease/histidine ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,425,9680TG 6.1% 579.4 / 26.1 165intergenic (‑52/+38)hisQ/hisJhistidine ABC transporter permease/histidine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (78/76);  new base G (7/3);  total (85/80)
Fisher's exact test for biased strand distribution p-value = 3.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.77e-01

Only 100 of 167 total aligned reads displayed.

TTAAACCGATGATTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGTTCGTTA  >  NC_000913/2425820‑2426114
                                                                                                                                                    |                                                                                                                                                  
ttAAACCGATGATTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGt                                                                                                                                                    >  3:718093/1‑149 (MQ=255)
 tAAACCGATGATTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGtt                                                                                                                                                   <  1:165977/149‑1 (MQ=255)
  aaaCCGATGATTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTa                                                                                                                                                  >  4:752773/1‑149 (MQ=255)
  aaaCCGATGATTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTa                                                                                                                                                  <  3:612944/149‑1 (MQ=255)
   aaCCGATGATTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTAc                                                                                                                                                 <  3:341057/149‑1 (MQ=255)
        atgatTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGaa                                                                                                                                            <  3:1374433/149‑1 (MQ=255)
        atgatTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGaa                                                                                                                                            <  2:105779/149‑1 (MQ=255)
        atgatTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGATACCCGaa                                                                                                                                            <  3:1344636/149‑1 (MQ=255)
                 gCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCggg                                                                                                                                   <  2:1044339/149‑1 (MQ=255)
                       aCTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctttct                                                                                                                             <  2:412166/149‑1 (MQ=255)
                       aCTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctttct                                                                                                                             <  2:413471/149‑1 (MQ=255)
                       aCTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctttct                                                                                                                             <  3:511808/149‑1 (MQ=255)
                       aCTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctttct                                                                                                                             <  4:718093/149‑1 (MQ=255)
                        cTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctttctt                                                                                                                            >  2:1480910/1‑149 (MQ=255)
                         tACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctttcttt                                                                                                                           <  2:73682/149‑1 (MQ=255)
                         tACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctttcttt                                                                                                                           <  3:752773/149‑1 (MQ=255)
                          aCAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCtttctttc                                                                                                                          >  2:214145/1‑149 (MQ=255)
                            agagCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCAc                                                                                                                        >  1:797078/1‑149 (MQ=255)
                               gCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGa                                                                                                                     >  4:344773/1‑149 (MQ=255)
                                  gATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATgg                                                                                                                  >  4:1271988/1‑149 (MQ=255)
                                     aGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGa                                                                                                               <  3:763397/149‑1 (MQ=255)
                                       ccAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGTGGGCTTTCTTTCACCGATGGCGAtt                                                                                                             >  1:580450/1‑149 (MQ=255)
                                       ccAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGAtt                                                                                                             >  4:497947/1‑149 (MQ=255)
                                        cAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTa                                                                                                            >  1:1230335/1‑149 (MQ=255)
                                            tCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGcca                                                                                                        >  3:211612/1‑149 (MQ=255)
                                            tCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGcca                                                                                                        <  2:270774/149‑1 (MQ=255)
                                            tCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGcca                                                                                                        >  1:758241/1‑149 (MQ=255)
                                            tCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGcca                                                                                                        >  1:587562/1‑149 (MQ=255)
                                               aGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGccacca                                                                                                     <  1:836074/149‑1 (MQ=255)
                                                gtgTGACGAGCGCACCCTGTAAAATAACACCTGAGAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCAt                                                                                                    <  3:1189933/147‑1 (MQ=255)
                                                     aCGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACa                                                                                               <  2:1422872/149‑1 (MQ=255)
                                                       gAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATc                                                                                             >  1:1124173/1‑149 (MQ=255)
                                                            cACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAAt                                                                                        <  4:54560/149‑1 (MQ=255)
                                                               ccTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGa                                                                                     >  2:750083/1‑149 (MQ=255)
                                                                cTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGaa                                                                                    <  3:459589/149‑1 (MQ=255)
                                                                cTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGaa                                                                                    <  2:77981/149‑1 (MQ=255)
                                                                 tGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctt                                                                                                                                >  3:959139/1‑104 (MQ=255)
                                                                 tGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctt                                                                                                                                <  4:959139/104‑1 (MQ=255)
                                                                  gTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGt                                                                                  >  3:1357297/1‑149 (MQ=255)
                                                                    aaaaTAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTAc                                                                                <  4:144473/149‑1 (MQ=255)
                                                                      aaTAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGCGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACtt                                                                              >  1:258394/1‑149 (MQ=255)
                                                                        tAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACtttt                                                                            >  3:612986/1‑149 (MQ=255)
                                                                            acCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGc                                                                        <  2:1081809/149‑1 (MQ=255)
                                                                             ccTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGAtt                                                                                                             >  1:662315/1‑111 (MQ=255)
                                                                             ccTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGAtt                                                                                                             <  2:662315/111‑1 (MQ=255)
                                                                              cTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGaa                                                                                    <  2:1293783/135‑1 (MQ=255)
                                                                              cTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGaa                                                                                    >  1:1293783/1‑135 (MQ=255)
                                                                               tGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTaa                                                                     >  3:977406/1‑149 (MQ=255)
                                                                                    aCCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATg                                                                                                                   >  4:58211/1‑98 (MQ=255)
                                                                                    aCCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATg                                                                                                                   <  3:58211/98‑1 (MQ=255)
                                                                                      ccATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGAt                                                                                                                    >  1:933062/1‑95 (MQ=255)
                                                                                      ccATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAAc                                                                                                <  2:850506/115‑1 (MQ=255)
                                                                                       cATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGaa                                                                                    >  1:472356/1‑126 (MQ=255)
                                                                                       cATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGaa                                                                                    <  2:472356/126‑1 (MQ=255)
                                                                                       cATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGt                                                             >  4:23798/1‑149 (MQ=255)
                                                                                            aacaTGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTa                                                        >  4:682931/1‑149 (MQ=255)
                                                                                             acaTGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTAc                                                       <  3:553952/149‑1 (MQ=255)
                                                                                                  ctgcCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCa                                                  <  1:717994/149‑1 (MQ=255)
                                                                                                    gcCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAATCGaa                                                                                    <  4:1154481/112‑1 (MQ=255)
                                                                                                     cctgtcctgtCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAgcg                                               >  2:992438/1‑149 (MQ=255)
                                                                                                       tgtcctgtcGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAgcgcg                                             >  1:1298876/1‑149 (MQ=255)
                                                                                                        gtcctgtcGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAgcgcgc                                            <  4:1357297/149‑1 (MQ=255)
                                                                                                         tcctgtcGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCAGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCa                                           >  1:1165006/1‑149 (MQ=255)
                                                                                                           ctgtcGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCAtt                                         >  3:1417664/1‑149 (MQ=255)
                                                                                                           ctgtcGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCAtt                                         >  1:90065/1‑149 (MQ=255)
                                                                                                             gtcGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTc                                       <  3:99879/149‑1 (MQ=255)
                                                                                                             gtcGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTc                                       <  4:281896/149‑1 (MQ=255)
                                                                                                              tcGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCg                                      <  4:612986/149‑1 (MQ=255)
                                                                                                               cGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCgg                                     <  1:992438/149‑1 (MQ=255)
                                                                                                                  gtgtgGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCaa                                  >  1:261981/1‑149 (MQ=255)
                                                                                                                    gtggtgAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAg                                <  1:214145/149‑1 (MQ=255)
                                                                                                                     tggtgAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAgg                               >  1:778538/1‑149 (MQ=255)
                                                                                                                       gtgAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAg                                >  3:1139083/1‑146 (MQ=255)
                                                                                                                       gtgAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAg                                <  4:1139083/146‑1 (MQ=255)
                                                                                                                       gtgAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCt                             <  1:791730/149‑1 (MQ=255)
                                                                                                                        tgAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCtt                            <  1:1480910/149‑1 (MQ=255)
                                                                                                                         gAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCttt                           >  2:119812/1‑149 (MQ=255)
                                                                                                                          aaTTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCgg                                     <  1:647611/138‑1 (MQ=255)
                                                                                                                              aCCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCa                      <  3:561015/149‑1 (MQ=255)
                                                                                                                                cGCCTCTTGTCTCCCTCCGGTTACCCGTAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTtctc                                                               <  4:1049388/106‑1 (MQ=255)
                                                                                                                                cGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTtctc                                                               >  3:1049388/1‑106 (MQ=255)
                                                                                                                                cGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGt                    <  4:977406/149‑1 (MQ=255)
                                                                                                                                cGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGt                    >  2:1230534/1‑149 (MQ=255)
                                                                                                                                   ctctTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCt                 >  3:1010720/1‑149 (MQ=255)
                                                                                                                                     ctTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTc               >  2:812818/1‑149 (MQ=255)
                                                                                                                                       ccgctcccttcGGGTAACCGGAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCg                                                              <  3:567796/97‑1 (MQ=38)
                                                                                                                                       ccgctcccttcGGGTAACCGGAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCg                                                              >  4:567796/4‑100 (MQ=38)
                                                                                                                                        cgctcccttcGGGTAACCGGAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAAtt                                                                   <  2:1326271/92‑1 (MQ=38)
                                                                                                                                        cgctcccttcGGGTAACCGGAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAAtt                                                                   >  1:1326271/3‑94 (MQ=38)
                                                                                                                                          ctccctccGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCa          >  2:619286/1‑149 (MQ=255)
                                                                                                                                           tcccttcGGGTAACCGGAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGt                         <  1:61710/133‑1 (MQ=255)
                                                                                                                                           tcccttcGGGTAACCGGAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGt                         >  2:61710/1‑133 (MQ=255)
                                                                                                                                           tcccttcGGGTAACCGGAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAg         >  3:513574/1‑149 (MQ=255)
                                                                                                                                           tccctccGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCtt                >  4:109516/1‑142 (MQ=255)
                                                                                                                                             ccttcGGGTAACCGGAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTtctc                                                               >  1:271023/1‑93 (MQ=255)
                                                                                                                                             cctccGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGtt       >  3:482250/1‑149 (MQ=255)
                                                                                                                                             cctccGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGtt       >  2:13377/1‑149 (MQ=255)
                                                                                                                                              ctccGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGTTc      >  3:1144074/1‑149 (MQ=255)
                                                                                                                                               tccGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGTTCg     >  2:834980/1‑149 (MQ=255)
                                                                                                                                                  ggTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGTTCGTTa  <  3:1433257/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TTAAACCGATGATTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGTTCGTTA  >  NC_000913/2425820‑2426114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: