Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,428,699 A→T 5.5% intergenic (‑73/+22) ubiX ← / ← purF 3‑octaprenyl‑4‑hydroxybenzoate carboxy‑lyase/amidophosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,428,6990AT 5.5% 627.1 / 19.4 180intergenic (‑73/+22)ubiX/purF3‑octaprenyl‑4‑hydroxybenzoate carboxy‑lyase/amidophosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (86/84);  new base T (5/5);  total (91/89)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01

Only 100 of 181 total aligned reads displayed.

GCGCAGAACCTGTAATAAGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACG  >  NC_000913/2428552‑2428847
                                                                                                                                                   |                                                                                                                                                    
  gcAGAACCTGTAATAAGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCg                                                                                                                                                   <  2:758189/149‑1 (MQ=255)
      aaCCTGTAATAAGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCAc                                                                                                                                               <  1:781965/149‑1 (MQ=255)
        ccTGTAATAAGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCGGTCTGGCAccc                                                                                                                                             <  1:1383901/149‑1 (MQ=255)
         cTGTAATAAGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCt                                                                                                                                            >  4:1086127/1‑149 (MQ=255)
         cTGTAATAAGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCt                                                                                                                                            >  4:155022/1‑149 (MQ=255)
         cTGTAATAAGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCt                                                                                                                                            >  4:180845/1‑149 (MQ=255)
         cTGTAATAAGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCt                                                                                                                                            >  1:599540/1‑149 (MQ=255)
         cTGTAATAAGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGCCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCt                                                                                                                                            >  4:606437/1‑149 (MQ=255)
            taataaGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAg                                                                                                                                         >  3:382455/1‑149 (MQ=255)
            taataaGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAg                                                                                                                                         >  3:375172/1‑149 (MQ=255)
            taataaGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAg                                                                                                                                         >  4:1257441/1‑149 (MQ=255)
            taataaGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGAGCCAGACCGGCACCCTCAg                                                                                                                                         >  4:379820/1‑149 (MQ=255)
                 aGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAg                                                                                                                                    <  4:1034858/149‑1 (MQ=255)
                    gcACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGGGAAGGca                                                                                                                                 >  3:1255699/1‑149 (MQ=255)
                           aTAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCt                                                                                                                          >  3:206770/1‑149 (MQ=255)
                           aTAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCt                                                                                                                          >  3:55398/1‑149 (MQ=255)
                            tAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCtt                                                                                                                         >  3:121093/1‑149 (MQ=255)
                             aaaTCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTc                                                                                                                        <  3:1218701/149‑1 (MQ=255)
                                     ccgcTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCa                                                                                                                >  3:532295/1‑149 (MQ=255)
                                       gcTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCAtt                                                                                                              <  3:1086127/149‑1 (MQ=255)
                                           gCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCga                                                                                                          >  3:771866/1‑149 (MQ=255)
                                             aCCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCgaga                                                                                                        >  3:449633/1‑149 (MQ=255)
                                             aCCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCgaga                                                                                                        >  1:373272/1‑149 (MQ=255)
                                             aCCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCgaga                                                                                                        >  3:1341621/1‑149 (MQ=255)
                                             aCCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCgaga                                                                                                        <  4:1305066/149‑1 (MQ=255)
                                              ccGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGAt                                                                                                       >  3:732018/1‑149 (MQ=255)
                                                gCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGAttt                                                                                                     >  2:137725/1‑149 (MQ=255)
                                                        cTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTc                                                                                             <  3:1315946/149‑1 (MQ=255)
                                                        cTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTc                                                                                             >  1:1156565/1‑149 (MQ=255)
                                                        cTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTc                                                                                             >  4:938320/1‑149 (MQ=255)
                                                         tACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCg                                                                                            >  2:480507/1‑149 (MQ=255)
                                                         tACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCg                                                                                            >  2:561759/1‑149 (MQ=255)
                                                                 gTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACg                                                                                    >  2:1409555/1‑149 (MQ=255)
                                                                 gTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACg                                                                                    >  1:733827/1‑149 (MQ=255)
                                                                  tCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGt                                                                                   >  3:842763/1‑149 (MQ=255)
                                                                    gTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTg                                                                                 <  3:1353712/149‑1 (MQ=255)
                                                                       tCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCAc                                                                              <  3:606437/149‑1 (MQ=255)
                                                                         aTAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTg                                                                            >  2:1152911/1‑149 (MQ=255)
                                                                          tAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGAtttt                                                                                                    <  2:529556/124‑1 (MQ=255)
                                                                          tAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGAtttt                                                                                                    >  1:529556/1‑124 (MQ=255)
                                                                          tAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGc                                                                           >  4:1179040/1‑149 (MQ=255)
                                                                           aaaTAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCt                                                                          >  3:171797/1‑149 (MQ=255)
                                                                           aaaTAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCt                                                                          >  3:325248/1‑149 (MQ=255)
                                                                             aTAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCttt                                                                        <  2:951462/149‑1 (MQ=255)
                                                                             aTAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCttt                                                                        <  1:911043/149‑1 (MQ=255)
                                                                               aGTTGCCCTGTCAGACTTGCAGACAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTCCACTGCTTTgg                                                                      <  3:826093/149‑1 (MQ=255)
                                                                                   gCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGATGTTGCACTGCTTTGGCGtc                                                                  <  4:842763/149‑1 (MQ=255)
                                                                                      cTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGtcatc                                                               <  2:599540/149‑1 (MQ=255)
                                                                                        gTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTcatcat                                                             <  4:530573/149‑1 (MQ=255)
                                                                                           aGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTac                                                          >  4:309445/1‑149 (MQ=255)
                                                                                           aGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTac                                                          <  2:193655/149‑1 (MQ=255)
                                                                                           aGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTac                                                          <  1:597516/149‑1 (MQ=255)
                                                                                           aGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTac                                                          <  4:641300/149‑1 (MQ=255)
                                                                                            gACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTacg                                                         <  2:1336486/149‑1 (MQ=255)
                                                                                               ttGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTacgtaa                                                      >  2:71893/1‑149 (MQ=255)
                                                                                               ttGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTacgtaa                                                      >  3:1281025/1‑149 (MQ=255)
                                                                                               ttGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTacgtaa                                                      >  1:617032/1‑149 (MQ=255)
                                                                                                 gcaggcagACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTacgtaacg                                                    >  2:907059/1‑149 (MQ=255)
                                                                                                   aggcagACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGt                                                                   <  4:1453174/132‑1 (MQ=255)
                                                                                                   aggcagACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTacgtaacgta                                                  >  3:1142418/1‑149 (MQ=255)
                                                                                                   aggcagACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTacgtaacgta                                                  >  2:1313390/1‑149 (MQ=255)
                                                                                                     gcagACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATc                                                <  4:459383/149‑1 (MQ=255)
                                                                                                      cagACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCg                                               <  1:565794/149‑1 (MQ=255)
                                                                                                       agACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGa                                              <  4:1341621/149‑1 (MQ=255)
                                                                                                       agACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGa                                              <  4:217111/149‑1 (MQ=255)
                                                                                                        gACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAg                                             <  1:821872/149‑1 (MQ=255)
                                                                                                           tttGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGaa                                          >  4:546146/1‑149 (MQ=255)
                                                                                                            ttGCAGGGTTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACGTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGaaa                                         <  1:952375/149‑1 (MQ=255)
                                                                                                            ttGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGaaa                                         <  3:180845/149‑1 (MQ=255)
                                                                                                               cAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCg                                      <  4:532295/149‑1 (MQ=255)
                                                                                                                aGGATTTCGGCGGGAGTTGCGAGTCAGGGTGCCAGACCGGCACTCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGa                                     <  4:287457/149‑1 (MQ=255)
                                                                                                                 ggATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAg                                    <  1:1034710/149‑1 (MQ=255)
                                                                                                                 ggATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAg                                    <  1:1287887/149‑1 (MQ=255)
                                                                                                                 ggATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAg                                    <  2:371627/149‑1 (MQ=255)
                                                                                                                 ggATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAg                                    <  3:286542/149‑1 (MQ=255)
                                                                                                                 ggATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAg                                    <  2:489304/149‑1 (MQ=255)
                                                                                                                 ggATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAg                                    <  2:655180/149‑1 (MQ=255)
                                                                                                                         gcggGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCt                            <  2:823435/149‑1 (MQ=255)
                                                                                                                                ttGCAAGTCAGGGTGCCAGACCGGCACCCTCGGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTATCGTATCGAGGAAATCGAGGTATCCCTGATAAAc                     >  4:1108614/1‑149 (MQ=255)
                                                                                                                                  ttcgctgaGGGTGCCGGTCTGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACAt                   >  3:889396/8‑149 (MQ=255)
                                                                                                                                   cAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATc                  <  4:171797/149‑1 (MQ=255)
                                                                                                                                     gctgaGGGTGCCGGTCTGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTac                                                          <  2:863820/103‑1 (MQ=255)
                                                                                                                                     gctgaGGGTGCCGGTCTGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTac                                                          >  1:863820/5‑107 (MQ=255)
                                                                                                                                      gTCAGGGTGCCAGACCGGCACTCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCttt               <  1:907059/149‑1 (MQ=255)
                                                                                                                                      gTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCttt               >  2:754157/1‑149 (MQ=255)
                                                                                                                                      gTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCttt               <  4:771866/149‑1 (MQ=255)
                                                                                                                                      gTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCttt               >  2:450665/1‑149 (MQ=255)
                                                                                                                                      gTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCttt               <  3:290584/149‑1 (MQ=255)
                                                                                                                                      gTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCttt               <  1:479033/149‑1 (MQ=255)
                                                                                                                                       tgaGGGTGCCGGTCTGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTg              >  1:684975/3‑149 (MQ=255)
                                                                                                                                       tCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTg              <  4:737760/149‑1 (MQ=255)
                                                                                                                                       tCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTg              <  3:513829/149‑1 (MQ=255)
                                                                                                                                        gaGGGTGCCGGTCTGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCt                            >  1:981744/2‑134 (MQ=255)
                                                                                                                                        gaGGGTGCCGGTCTGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCt                            <  2:981744/133‑1 (MQ=255)
                                                                                                                                            gTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGAc         <  4:273561/149‑1 (MQ=255)
                                                                                                                                            gTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGACCAACATCTTTGGTGAc         <  4:849293/149‑1 (MQ=255)
                                                                                                                                            gTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAAGTAGCCCTGATCAACATCTTTAGTGAc         >  4:942294/1‑149 (MQ=255)
                                                                                                                                               ccAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGCTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGCGGCAATCGAGGTCGCCCTGATCAACATCTTTGGTGACGTa      <  1:1020963/149‑1 (MQ=255)
                                                                                                                                               ccAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa      <  1:1113957/149‑1 (MQ=255)
                                                                                                                                                   aCCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACg  >  2:1004717/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCGCAGAACCTGTAATAAGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACG  >  NC_000913/2428552‑2428847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: