Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 314,258 G→A 6.1% intergenic (‑453/‑99) ykgP ← / → eaeH pseudogene, oxidoreductase family/pseudogene, attaching and effacing protein homology;factor; Not classified

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913314,2580GA 6.1% 472.9 / 11.4 144intergenic (‑453/‑99)ykgP/eaeHpseudogene, oxidoreductase family/pseudogene, attaching and effacing protein homology;factor; Not classified
Reads supporting (aligned to +/- strand):  ref base G (70/65);  new base A (5/4);  total (75/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

TATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTATTCAGT  >  NC_000913/314114‑314406
                                                                                                                                                |                                                                                                                                                    
tATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGaaa                                                                                                                                                  >  2:875720/1‑149 (MQ=255)
tATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGaaa                                                                                                                                                  >  1:868110/1‑149 (MQ=255)
 aTTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGaaaa                                                                                                                                                 <  1:1429098/149‑1 (MQ=255)
 aTTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGaaaa                                                                                                                                                 <  2:1461954/149‑1 (MQ=255)
    tAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTCTCCGCAGAAAAGGa                                                                                                                                              <  1:603793/149‑1 (MQ=255)
      aTGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCCGCAGAAAAGGaaa                                                                                                                                            <  2:289573/149‑1 (MQ=255)
      aTGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCCGCAGAAAAGGaaa                                                                                                                                            <  3:338580/149‑1 (MQ=255)
      aTGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCCGCAGAAAAAGaaa                                                                                                                                            >  3:207423/1‑149 (MQ=255)
          cTTACACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCgg                                                                                                                                        <  1:470179/149‑1 (MQ=255)
               acaAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAAt                                                                                                                                   >  1:989672/1‑149 (MQ=255)
                caAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATc                                                                                                                                  >  1:1331481/1‑149 (MQ=255)
                caAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATc                                                                                                                                  >  1:518071/1‑149 (MQ=255)
                 aaaaaGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCCGCAGAAAAGGAAAtacc                                                                                                                                        >  4:185465/1‑139 (MQ=255)
                   aaaGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATcccc                                                                                                                               <  3:1098187/149‑1 (MQ=255)
                      gCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGt                                                                                                                            >  4:521568/1‑149 (MQ=255)
                       cTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGtt                                                                                                                           >  2:770060/1‑149 (MQ=255)
                        tAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGttt                                                                                                                          >  4:393911/1‑149 (MQ=255)
                         aaaTTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGtttt                                                                                                                         >  2:403940/1‑149 (MQ=255)
                          aaTTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGttttt                                                                                                                        >  4:1253139/1‑149 (MQ=255)
                                   ccATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGc                                                                                                               <  2:386658/149‑1 (MQ=255)
                                   ccATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGc                                                                                                               >  1:386658/1‑149 (MQ=255)
                                    cATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCgg                                                                                                                                        >  3:582982/1‑123 (MQ=255)
                                     ataaataaAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAg                                                                                                             >  4:423917/1‑149 (MQ=255)
                                      taaataaaAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGa                                                                                                            <  2:585719/149‑1 (MQ=255)
                                         ataaaAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGc                                                                                                         >  1:443545/1‑149 (MQ=255)
                                          taaaAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGcc                                                                                                        >  4:255778/1‑149 (MQ=255)
                                          taaaAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGcc                                                                                                        >  1:985924/1‑149 (MQ=255)
                                          taaaAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGcc                                                                                                        >  1:567943/1‑149 (MQ=255)
                                            aaaaTATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCat                                                                                                      <  2:81936/149‑1 (MQ=255)
                                             aaaTATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCatt                                                                                                     >  4:1452581/1‑149 (MQ=255)
                                             aaaTATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCatt                                                                                                     >  2:138230/1‑149 (MQ=255)
                                                 ataATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTAttt                                                                                                 <  3:651767/149‑1 (MQ=255)
                                                   aaTCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTtgt                                                                                               <  4:815441/149‑1 (MQ=255)
                                                       ccGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCt                                                                                           >  4:571467/1‑149 (MQ=255)
                                                        cgcgCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCGGACGCCATTATTTGTGTCTg                                                                                          >  3:1199981/1‑149 (MQ=255)
                                                               aaCCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGt                                                                                   >  2:475503/1‑149 (MQ=255)
                                                                accaccTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTATGCCTATGtt                                                                                  >  1:798218/1‑149 (MQ=255)
                                                                 ccaccTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTc                                                                                 <  1:138230/149‑1 (MQ=255)
                                                                   accTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGt                                                                               <  4:1284038/149‑1 (MQ=255)
                                                                   accTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGt                                                                               >  4:830166/1‑149 (MQ=255)
                                                                       gatgaGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAAt                                                                           <  2:1094341/149‑1 (MQ=255)
                                                                        atgaGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAAtt                                                                          <  2:1331481/149‑1 (MQ=255)
                                                                         tgaGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTc                                                                         <  3:571467/149‑1 (MQ=255)
                                                                          gaGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGt                                                                                   <  1:1280925/138‑1 (MQ=255)
                                                                            gTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCgg                                                                                                                                        <  1:1086207/83‑1 (MQ=255)
                                                                            gTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCgg                                                                                                                                        >  2:1086207/1‑83 (MQ=255)
                                                                            gTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTAttt                                                                                                 <  2:538533/122‑1 (MQ=255)
                                                                            gTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTAttt                                                                                                 >  1:538533/1‑122 (MQ=255)
                                                                            gTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGtt                                                                      <  4:56996/149‑1 (MQ=255)
                                                                             tGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTtc                                                                     <  3:463042/149‑1 (MQ=255)
                                                                                cTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGGCTATGTTCGTTAATTCGTtcatc                                                                  <  3:220705/149‑1 (MQ=255)
                                                                                 tataGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTtcatca                                                                 <  1:344060/149‑1 (MQ=255)
                                                                                  ataGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCt                                                                                                                      <  4:115763/95‑1 (MQ=255)
                                                                                  ataGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCt                                                                                                                      >  3:115763/1‑95 (MQ=255)
                                                                                  ataGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAg                                                                <  1:875720/149‑1 (MQ=255)
                                                                                        aCTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTGCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACCAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGt                                                                       <  2:876372/136‑1 (MQ=255)
                                                                                         cTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTa                                                         >  3:721991/1‑149 (MQ=255)
                                                                                           ggATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTAtc                                                       <  2:619357/149‑1 (MQ=255)
                                                                                              tatatTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCa                                                    >  2:777240/1‑149 (MQ=255)
                                                                                              tatatTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCa                                                    >  3:983166/1‑149 (MQ=255)
                                                                                                tatTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAAt                                                  <  4:983166/149‑1 (MQ=255)
                                                                                                tatTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGGTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAAt                                                  >  3:448652/1‑149 (MQ=255)
                                                                                                 atTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGaaaa                                                                                                                                                 <  4:67810/53‑1 (MQ=255)
                                                                                                 atTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGaaaa                                                                                                                                                 >  3:67810/1‑53 (MQ=255)
                                                                                                 atTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTaa                                                                            >  3:1027446/1‑122 (MQ=255)
                                                                                                 atTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTaa                                                                            <  4:1027446/122‑1 (MQ=255)
                                                                                                    aGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTca                                              <  2:985924/149‑1 (MQ=255)
                                                                                                         gCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATta                                         <  1:795837/149‑1 (MQ=255)
                                                                                                            gTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATaa                                      <  3:1452581/149‑1 (MQ=255)
                                                                                                            gTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATaa                                      >  2:468704/1‑149 (MQ=255)
                                                                                                            gTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATaa                                      <  3:361497/149‑1 (MQ=255)
                                                                                                              gCACTTTCTTGCATAAAGGTATTTCCTTTTCTGCGGGAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGGCTGCCTATGTTCGTTAATTTGTTCATCAGGAAATTATCTCAATGTCACATTATaaaa                                    >  2:404387/1‑149 (MQ=255)
                                                                                                                aCTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACa                                  <  3:1408456/149‑1 (MQ=255)
                                                                                                                aCTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACa                                  <  4:935496/149‑1 (MQ=255)
                                                                                                                aCTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACa                                  >  4:509797/1‑149 (MQ=255)
                                                                                                                aCTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACa                                  <  2:1022527/149‑1 (MQ=255)
                                                                                                                  tttCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTtcatc                                                                  <  1:769813/115‑1 (MQ=255)
                                                                                                                  tttCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAgg                                >  2:226288/1‑149 (MQ=255)
                                                                                                                  tttCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAgg                                >  3:1035256/1‑149 (MQ=255)
                                                                                                                  tttCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAgg                                <  1:112761/149‑1 (MQ=255)
                                                                                                                  tttCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAgg                                >  1:303164/1‑149 (MQ=255)
                                                                                                                   ttCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGt                               <  3:521568/149‑1 (MQ=255)
                                                                                                                      ttACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCAt                            >  2:1252305/1‑149 (MQ=255)
                                                                                                                          aTAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAaac                        >  4:852331/1‑149 (MQ=255)
                                                                                                                            aaaGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAaacaa                      <  3:1253139/149‑1 (MQ=255)
                                                                                                                             aaGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAaacaac                     >  1:145483/1‑149 (MQ=255)
                                                                                                                              aGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAAcc                    <  2:1471236/149‑1 (MQ=255)
                                                                                                                                   tttCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGAt               >  2:764785/1‑149 (MQ=255)
                                                                                                                                   tttCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGAt               >  3:527144/1‑149 (MQ=255)
                                                                                                                                     tCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGt                               <  3:1003647/131‑1 (MQ=255)
                                                                                                                                     tCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGt                               >  4:1003647/1‑131 (MQ=255)
                                                                                                                                     tCCTTTTCCGCAGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGAttt             >  2:837633/1‑149 (MQ=255)
                                                                                                                                     tCCTTTTCCGCAGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGAttt             >  2:1222332/1‑149 (MQ=255)
                                                                                                                                      ccTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTc            <  4:1226763/149‑1 (MQ=255)
                                                                                                                                        ttttCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTGTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGt          >  3:1376185/1‑149 (MQ=255)
                                                                                                                                        ttttCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGt          >  1:701186/1‑149 (MQ=255)
                                                                                                                                        ttttCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGt          >  4:1291717/1‑149 (MQ=255)
                                                                                                                                            cTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTAtt      <  1:770060/149‑1 (MQ=255)
                                                                                                                                                ggAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTATTCAGt  >  3:1276289/1‑149 (MQ=255)
                                                                                                                                                ggAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTATTCAGt  >  1:100504/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTATTCAGT  >  NC_000913/314114‑314406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: