Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,638,584 A→T 5.9% F23L (TTT→TTA bamB ← BamABCDE complex OM biogenesis lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,638,5840AT 5.9% 546.5 / 16.5 153F23L (TTT→TTAbamBBamABCDE complex OM biogenesis lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (68/76);  new base T (3/6);  total (71/82)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

Only 100 of 159 total aligned reads displayed.

AGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCA  >  NC_000913/2638436‑2638730
                                                                                                                                                    |                                                                                                                                                  
aGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTa                                                                                                                                                    >  1:1315562/1‑149 (MQ=255)
  aCAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTaaa                                                                                                                                                  <  2:212472/149‑1 (MQ=255)
  aCAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTaaa                                                                                                                                                  >  3:1130991/1‑149 (MQ=255)
    gaCGTTGTCCGCCAGTGCCGGATGGAGATTGGAAAAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGTCCGGCGTGAACAGGATTTCAACGGTGGGCATTGGGGACATCTTTACCTCATCTTCTGCGCTGTTAAAct                                                                                                                                                >  2:1123333/2‑148 (MQ=255)
    aaCGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGTAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACa                                                                                                                                                >  1:1347329/1‑149 (MQ=255)
     aCGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAg                                                                                                                                               <  3:201823/149‑1 (MQ=255)
     aCGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAg                                                                                                                                               <  1:367977/149‑1 (MQ=255)
      cGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGc                                                                                                                                              <  1:1418135/149‑1 (MQ=255)
         tGTCCGGCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAAGTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACGTCTTCTTCGCTGTTTAACAGCGaa                                                                                                                                           <  4:708164/149‑1 (MQ=255)
          gTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAAc                                                                                                                                          >  2:673366/1‑149 (MQ=255)
           tCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACa                                                                                                                                         <  4:290790/149‑1 (MQ=255)
              gCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGcc                                                                                                                                      >  4:738022/1‑149 (MQ=255)
               ccAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTTAACAGCGaagaagat                                                                                                                                     <  3:166087/149‑7 (MQ=255)
               ccAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCg                                                                                                                                     <  4:1131722/149‑1 (MQ=255)
               ccAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCg                                                                                                                                     >  2:728356/1‑149 (MQ=255)
                      cGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGTAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTaaaa                                                                                                                              <  2:1347329/149‑1 (MQ=255)
                      cGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTaaaa                                                                                                                              <  1:25975/149‑1 (MQ=255)
                       ggATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAg                                                                                                                             >  2:1314757/1‑149 (MQ=255)
                        gATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAgg                                                                                                                            <  3:1088000/149‑1 (MQ=255)
                        gATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAgg                                                                                                                            <  2:541957/149‑1 (MQ=255)
                           gAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGTTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTa                                                                                                                         >  3:1160625/1‑149 (MQ=255)
                           gAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTa                                                                                                                         >  1:781524/1‑149 (MQ=255)
                                  ggAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGaaa                                                                                                                  <  2:53798/149‑1 (MQ=255)
                                    aaTAGAAGTTGCCAATGCCGCTGCCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTTAACAGCGaaga                                                                                                                                         <  3:916083/124‑3 (MQ=255)
                                    aaTAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGTGACATCTTTACCACATCTTCTTCGCTGTTTAACAGCGaaga                                                                                                                                         >  4:916083/1‑122 (MQ=255)
                                     aTAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCa                                                                                                               >  3:1275518/1‑149 (MQ=255)
                                     aTAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCa                                                                                                               >  2:420595/1‑149 (MQ=255)
                                     aTAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCa                                                                                                               <  3:636741/149‑1 (MQ=255)
                                      tAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAg                                                                                                              >  1:1330422/1‑149 (MQ=255)
                                        gAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTc                                                                                                            >  1:25004/1‑149 (MQ=255)
                                         aaGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTcc                                                                                                           >  1:817930/1‑149 (MQ=255)
                                         aaGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCATAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTcc                                                                                                           <  3:1201311/149‑1 (MQ=255)
                                         aaGTTGCCAATGCCGCTAACAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTcc                                                                                                           >  2:251043/1‑149 (MQ=255)
                                          aGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCt                                                                                                          <  3:11200/149‑1 (MQ=255)
                                           gtagCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTACCACATCTTCTTCGCTGTTAAACAGCGTACAGCCGCTTAAAAgg                                                                                                                            >  3:971973/4‑129 (MQ=255)
                                               ccAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGgcag                                                                                                     <  1:981551/149‑1 (MQ=255)
                                                 aaTGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGgcagca                                                                                                   >  2:566972/1‑149 (MQ=255)
                                                 aaTGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGgcagca                                                                                                   <  2:1340339/149‑1 (MQ=255)
                                                  aTGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGcagcag                                                                                                  <  2:1482492/149‑1 (MQ=255)
                                                      cGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAAt                                                                                              <  3:898362/149‑1 (MQ=255)
                                                      cGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGACGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAAt                                                                                              <  1:902950/149‑1 (MQ=255)
                                                           ccAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACg                                                                                         <  1:1127090/149‑1 (MQ=255)
                                                            cAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAg                                                                                                                                               <  3:703951/94‑1 (MQ=255)
                                                              aCGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCaa                                                                                      >  2:933169/1‑149 (MQ=255)
                                                                ggAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAAtt                                                                                    <  2:40897/149‑1 (MQ=255)
                                                                ggAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAAtt                                                                                    <  4:419374/149‑1 (MQ=255)
                                                                       cTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCgg                                                                             <  3:738022/149‑1 (MQ=255)
                                                                        tCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCggg                                                                            >  2:210711/1‑149 (MQ=255)
                                                                          cACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTc                                                                          <  1:1138014/149‑1 (MQ=255)
                                                                          cACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTc                                                                          <  4:528111/149‑1 (MQ=255)
                                                                           aCGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTcc                                                                         >  3:664799/1‑149 (MQ=255)
                                                                             gCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCct                                                                       <  2:618552/149‑1 (MQ=255)
                                                                                    tCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGAt                                                                >  3:955799/1‑149 (MQ=255)
                                                                                    tCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGAt                                                                <  1:210711/149‑1 (MQ=255)
                                                                                    tCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGAt                                                                <  3:1342850/149‑1 (MQ=255)
                                                                                    acGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGAt                                                                <  4:822367/148‑1 (MQ=255)
                                                                                     cGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATg                                                               <  1:46275/149‑1 (MQ=255)
                                                                                       gCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGa                                                             <  1:538806/149‑1 (MQ=255)
                                                                                       gCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGa                                                             <  2:1330422/149‑1 (MQ=255)
                                                                                         gTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACa                                                           >  2:1097071/1‑149 (MQ=255)
                                                                                            aaCTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAat                                                        <  1:1472030/149‑1 (MQ=255)
                                                                                             aCTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAatt                                                       >  1:681121/1‑149 (MQ=255)
                                                                                             aCTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAatt                                                       <  4:132617/149‑1 (MQ=255)
                                                                                               tGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGcagcag                                                                                                  <  4:1195730/104‑1 (MQ=255)
                                                                                               tGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATg                                                               <  4:664817/139‑1 (MQ=255)
                                                                                               tGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAattat                                                     <  4:1049683/149‑1 (MQ=255)
                                                                                               tGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAattat                                                     >  2:1431647/1‑149 (MQ=255)
                                                                                               tGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAattat                                                     >  2:1295660/1‑149 (MQ=255)
                                                                                                 gTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAAttatta                                                   >  3:373230/1‑149 (MQ=255)
                                                                                                  ttttCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTaa                                                  >  2:1399915/1‑149 (MQ=255)
                                                                                                       aaCGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTc                                             >  3:868837/1‑149 (MQ=255)
                                                                                                       aaCGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTc                                             >  2:1427097/1‑149 (MQ=255)
                                                                                                        aCGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCa                                            <  4:1347654/149‑1 (MQ=255)
                                                                                                        aCGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCa                                            <  2:681121/149‑1 (MQ=255)
                                                                                                           gTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCt                                         <  3:1211322/149‑1 (MQ=255)
                                                                                                              ggCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGca                                      <  4:866973/149‑1 (MQ=255)
                                                                                                                 aaTGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGcatca                                   <  2:603690/149‑1 (MQ=255)
                                                                                                                  aTGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGcatcat                                  >  1:42134/1‑149 (MQ=255)
                                                                                                                       gACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGc                             >  1:1481581/1‑149 (MQ=255)
                                                                                                                       gACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGc                             >  4:1018624/1‑149 (MQ=255)
                                                                                                                        aCATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCt                            >  4:171750/1‑149 (MQ=255)
                                                                                                                        aCATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCt                            <  3:1018624/149‑1 (MQ=255)
                                                                                                                          aTCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCa                          <  3:551822/149‑1 (MQ=255)
                                                                                                                          aTCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCa                          >  1:321489/1‑149 (MQ=255)
                                                                                                                                  cacaTCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGa                  <  1:1474163/149‑1 (MQ=255)
                                                                                                                                  cacaTCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGa                  <  1:1431647/149‑1 (MQ=255)
                                                                                                                                  cacaTCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGa                  <  4:1270500/149‑1 (MQ=255)
                                                                                                                                  cacaTCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGa                  >  1:723490/1‑149 (MQ=255)
                                                                                                                                  cacaTCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGa                  >  3:1297384/1‑149 (MQ=255)
                                                                                                                                  cacaTCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGa                  >  1:1255224/1‑149 (MQ=255)
                                                                                                                                     aTCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTa               <  2:1481581/149‑1 (MQ=255)
                                                                                                                                        ttcttcGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACa            >  3:50554/1‑149 (MQ=255)
                                                                                                                                        ttcttcGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACa            >  1:25021/1‑149 (MQ=255)
                                                                                                                                        ctgttcGCTGTTTAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAAttatta                                                   >  4:5941/4‑110 (MQ=255)
                                                                                                                                           ttcGCTGTTTAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGa                  >  2:1227651/1‑140 (MQ=255)
                                                                                                                                           ttcGCTGTTTAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGa                  <  1:1227651/140‑1 (MQ=255)
                                                                                                                                             cGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCt       >  1:881824/1‑149 (MQ=255)
                                                                                                                                              gCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCtt      <  2:8599/149‑1 (MQ=255)
                                                                                                                                               cTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCACTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCttt     >  1:215592/1‑149 (MQ=255)
                                                                                                                                                  ttAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATgg                                                              >  2:1374842/1‑89 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCA  >  NC_000913/2638436‑2638730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: