Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,822,673 C→T 7.0% intergenic (‑34/+35) recX ← / ← recA regulatory protein for RecA/DNA recombination and repair protein; ssDNA‑dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP‑dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,822,6730CT 7.0% 496.2 / 19.1 157intergenic (‑34/+35)recX/recAregulatory protein for RecA/DNA recombination and repair protein; ssDNA‑dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP‑dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor
Reads supporting (aligned to +/- strand):  ref base C (70/76);  new base T (7/4);  total (77/80)
Fisher's exact test for biased strand distribution p-value = 3.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.87e-01

Only 100 of 161 total aligned reads displayed.

GTGCCGCGAGTTTACGTCGCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTC  >  NC_000913/2822525‑2822819
                                                                                                                                                    |                                                                                                                                                  
 tGCCGCGAGTTTACGTCGCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAATAGGGcc                                                                                                                                                   <  2:1045791/149‑1 (MQ=255)
         gTTTACGTCGCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGc                                                                                                                                           >  3:1023405/1‑149 (MQ=255)
               gTCGCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCt                                                                                                                                     >  1:535700/1‑149 (MQ=255)
                 cGCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGTCGCATCTGCGGCCCttt                                                                                                                                   <  4:762155/149‑2 (MQ=255)
                 cGCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTtg                                                                                                                                   >  1:982727/1‑149 (MQ=255)
                 cGCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTtg                                                                                                                                   <  4:1254232/149‑1 (MQ=255)
                 cGCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGACGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTtg                                                                                                                                   >  3:1414930/1‑149 (MQ=255)
                  gCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTtgt                                                                                                                                  <  4:486162/149‑1 (MQ=255)
                  gCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTtgt                                                                                                                                  <  1:802817/149‑1 (MQ=255)
                  gCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTtgt                                                                                                                                  <  4:1177366/149‑1 (MQ=255)
                   cAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGGATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTtgtg                                                                                                                                 <  3:1104015/149‑1 (MQ=255)
                   cAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGcc                                                                                                                                                   <  1:1283413/131‑1 (MQ=255)
                   cAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGcc                                                                                                                                                   >  2:1283413/1‑131 (MQ=255)
                       tCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGTCGCATCTGCGGCCCttttgcttt                                                                                                                             <  3:622232/149‑8 (MQ=255)
                       tCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATc                                                                                                                             <  2:919636/149‑1 (MQ=255)
                       tCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATc                                                                                                                             >  1:553444/1‑149 (MQ=255)
                         ttGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTa                                                                                                                               >  2:538211/1‑145 (MQ=255)
                          tGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCaaa                                                                                                                          <  1:1470949/149‑1 (MQ=255)
                             tCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACaa                                                                                                                       >  2:114251/1‑149 (MQ=255)
                             tCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACaa                                                                                                                       >  2:905941/1‑149 (MQ=255)
                             tCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACaa                                                                                                                       >  4:1132571/1‑149 (MQ=255)
                             tCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACaa                                                                                                                       >  3:904842/1‑149 (MQ=255)
                               aCTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGTCGCATCTGCGGCCCttt                                                                                                                                   <  4:676937/135‑2 (MQ=255)
                               aCTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGTCGCATCTGCGGCCCttt                                                                                                                                   >  3:676937/1‑134 (MQ=255)
                               aCTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGa                                                                                                                     <  4:940918/149‑1 (MQ=255)
                               aCTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGa                                                                                                                     <  2:219988/149‑1 (MQ=255)
                               aCTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGa                                                                                                                     >  2:528228/1‑149 (MQ=255)
                                 tgtgATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACg                                                                                                                   >  1:25974/1‑149 (MQ=255)
                                   tgATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGAt                                                                                                                 <  2:1110104/149‑1 (MQ=255)
                                              cGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTc                                                                                                      <  4:122646/149‑1 (MQ=255)
                                               gCCGGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCg                                                                                                     >  3:1044529/1‑149 (MQ=255)
                                                  aGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTa                                                                                                  <  3:71748/149‑1 (MQ=255)
                                                          tACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGc                                                                                          <  2:507471/149‑1 (MQ=255)
                                                             cGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTAc                                                                                       >  1:732891/1‑149 (MQ=255)
                                                             cGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTAc                                                                                       >  1:286616/1‑149 (MQ=255)
                                                                aCGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGcc                                                                                    >  1:179843/1‑149 (MQ=255)
                                                                  gATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCtt                                                                                  <  2:1478577/149‑1 (MQ=255)
                                                                   aTCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTtgt                                                                                                                                  >  2:627173/1‑100 (MQ=255)
                                                                   aTCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTc                                                                                 <  1:242313/149‑1 (MQ=255)
                                                                    tCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCg                                                                                >  4:1371994/1‑149 (MQ=255)
                                                                       aaCAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTa                                                                             >  2:1021925/1‑149 (MQ=255)
                                                                       aaCAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCATTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTa                                                                             >  4:460040/1‑149 (MQ=255)
                                                                               aGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTAc                                                                     >  4:543420/1‑149 (MQ=255)
                                                                                  atatGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACaga                                                                  >  3:1352559/1‑149 (MQ=255)
                                                                                  atatGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACaga                                                                  <  1:758853/149‑1 (MQ=255)
                                                                                       ccGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAAt                                                             <  4:509438/149‑1 (MQ=255)
                                                                                         gggCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATcc                                                           <  2:736881/149‑1 (MQ=255)
                                                                                         gggCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATcc                                                           >  1:1293035/1‑149 (MQ=255)
                                                                                          ggCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCg                                                          >  2:648605/1‑149 (MQ=255)
                                                                                             gACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCg                                                       >  3:1326844/1‑149 (MQ=255)
                                                                                               cGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGtt                                                     <  1:1014606/149‑1 (MQ=255)
                                                                                                gggATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCt                                                                              >  4:133605/1‑123 (MQ=255)
                                                                                                gggATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCt                                                                              <  3:133605/123‑1 (MQ=255)
                                                                                                gggATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTg                                                    >  1:657993/1‑149 (MQ=255)
                                                                                                gggATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCATTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTg                                                    <  3:460040/149‑1 (MQ=255)
                                                                                                      ttGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGcc                                                                                    <  3:10169/111‑1 (MQ=255)
                                                                                                      ttGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGcc                                                                                    >  4:10169/1‑111 (MQ=255)
                                                                                                      ttGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCg                                              >  1:777157/1‑149 (MQ=255)
                                                                                                       tGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCgg                                             <  4:1018526/149‑1 (MQ=255)
                                                                                                          ttCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGtt                                          >  2:1149635/1‑149 (MQ=255)
                                                                                                            cTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGc                                        <  2:553444/149‑1 (MQ=255)
                                                                                                            cTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGc                                        >  1:294346/1‑149 (MQ=255)
                                                                                                              gTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTc                                      >  1:350512/1‑149 (MQ=255)
                                                                                                               tCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTca                                     >  4:1176474/1‑149 (MQ=255)
                                                                                                               tCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTca                                     >  2:911369/1‑149 (MQ=255)
                                                                                                                 aTGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTcagc                                   >  4:749499/1‑149 (MQ=255)
                                                                                                                  tGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCgg                                             >  1:910109/1‑138 (MQ=255)
                                                                                                                    gCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTc                                                                                 >  4:562830/1‑100 (MQ=255)
                                                                                                                    gCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTc                                                                                 <  3:562830/100‑1 (MQ=255)
                                                                                                                    gCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCagcagc                                <  1:54647/149‑1 (MQ=255)
                                                                                                                        atCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTc                                               >  1:803501/1‑130 (MQ=255)
                                                                                                                         tCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTc                           <  3:1000837/149‑1 (MQ=255)
                                                                                                                            ttACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTCTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACg                        >  2:1054216/1‑149 (MQ=255)
                                                                                                                             tACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGt                       >  2:1360864/1‑149 (MQ=255)
                                                                                                                                aaCTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACt                    <  1:648605/149‑1 (MQ=255)
                                                                                                                                  cTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACttt                  >  1:1464418/1‑149 (MQ=255)
                                                                                                                                   ttAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAAc                             >  2:290022/1‑137 (MQ=255)
                                                                                                                                   ttAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTttc                 >  4:162587/1‑149 (MQ=255)
                                                                                                                                   ttAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTttc                 >  3:1415949/1‑149 (MQ=255)
                                                                                                                                   ttAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTttc                 >  1:1372684/1‑149 (MQ=255)
                                                                                                                                     aaaaaaGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTcagca                                  >  1:294807/1‑130 (MQ=255)
                                                                                                                                     aaaaaaGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTcagca                                  <  2:294807/130‑1 (MQ=255)
                                                                                                                                     aaaaaaGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTttctt               <  3:1176474/149‑1 (MQ=255)
                                                                                                                                     aaaaaaGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTttctt               <  2:1464418/149‑1 (MQ=255)
                                                                                                                                       aaaaGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTtcttct             >  3:1192898/1‑149 (MQ=255)
                                                                                                                                        aaaGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTtctc            <  4:133212/149‑1 (MQ=255)
                                                                                                                                        aaaGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTtctc            <  2:939008/149‑1 (MQ=255)
                                                                                                                                          aGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGCGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGa          <  1:1021925/149‑1 (MQ=255)
                                                                                                                                          aGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGa          <  3:1161822/149‑1 (MQ=255)
                                                                                                                                             acaaGGGTCGCATCTGCGGCCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGAtct       >  4:1325735/3‑149 (MQ=255)
                                                                                                                                             acaaGGGTCGCATCTGCGGCCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGAtct       >  1:189173/3‑149 (MQ=255)
                                                                                                                                             aaaaGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTc                           >  1:689415/1‑129 (MQ=255)
                                                                                                                                             aaaaGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTc                           <  2:689415/129‑1 (MQ=255)
                                                                                                                                             aaaaGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGAtct       <  2:833098/149‑1 (MQ=255)
                                                                                                                                             aaaaGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGAtct       >  1:439559/1‑149 (MQ=255)
                                                                                                                                             aaaaGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGAtct       >  3:252557/1‑149 (MQ=255)
                                                                                                                                             aaaaGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGAtct       >  2:1221123/1‑149 (MQ=255)
                                                                                                                                               aaGGGTCGCATCTGCGGCCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATctct     >  2:992487/1‑149 (MQ=255)
                                                                                                                                                aGGGTCGCATCTGCGGCCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCtt    >  3:160036/1‑149 (MQ=255)
                                                                                                                                                aGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCtt    >  3:1142362/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GTGCCGCGAGTTTACGTCGCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTC  >  NC_000913/2822525‑2822819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: