Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,890,625 A→T 5.4% L425H (CTC→CAC)  cysJ ← sulfite reductase, alpha subunit, flavoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,890,6250AT 5.4% 511.0 / 12.5 145L425H (CTC→CAC) cysJsulfite reductase, alpha subunit, flavoprotein
Reads supporting (aligned to +/- strand):  ref base A (72/65);  new base T (3/5);  total (75/70)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 150 total aligned reads displayed.

GCGTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTA  >  NC_000913/2890477‑2890772
                                                                                                                                                    |                                                                                                                                                   
gcgTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGa                                                                                                                                                     <  2:685946/149‑1 (MQ=255)
gcgTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGa                                                                                                                                                     <  4:653988/149‑1 (MQ=255)
gcgTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGa                                                                                                                                                     <  3:290830/149‑1 (MQ=255)
   ttGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGa                                                                                                                                                  >  1:191832/1‑149 (MQ=255)
       tgcATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCTCGCTGACCGCGTGGAAgaa                                                                                                                                              <  3:542486/149‑3 (MQ=255)
       tgcATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCTCGCTGACCGCGTGGAAgaa                                                                                                                                              <  4:1130572/149‑3 (MQ=255)
       tgcATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCt                                                                                                                                              >  3:722196/1‑149 (MQ=255)
       tgcATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCt                                                                                                                                              <  2:1277397/149‑1 (MQ=255)
       tgcATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCt                                                                                                                                              >  4:147794/1‑149 (MQ=255)
          aTAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGa                                                                                                                                           >  1:1482746/1‑149 (MQ=255)
           tAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAg                                                                                                                                          <  2:191832/149‑1 (MQ=255)
           tAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAg                                                                                                                                          <  4:681619/149‑1 (MQ=255)
               ggCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCCGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATACATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCACCGAGGAAGCTGGAGGcac                                                                                                                                      <  3:1020657/149‑1 (MQ=255)
                  gcgGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGcaccac                                                                                                                                   >  4:1453248/1‑149 (MQ=255)
                  gcgGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGcaccac                                                                                                                                   >  2:592896/1‑149 (MQ=255)
                                tACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCgg                                                                                                                     >  2:231685/1‑149 (MQ=255)
                                 aCCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGc                                                                                                                    <  3:1226560/149‑1 (MQ=255)
                                   cGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCt                                                                                                                  >  1:110925/1‑149 (MQ=255)
                                   cGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCt                                                                                                                  <  4:464428/149‑1 (MQ=255)
                                    ggTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCtt                                                                                                                 <  4:530474/149‑1 (MQ=255)
                                      tGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTcc                                                                                                               <  2:3246/149‑1 (MQ=255)
                                       gCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCa                                                                                                              <  4:24574/149‑1 (MQ=255)
                                          tGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCAcgt                                                                                                           >  1:659307/1‑149 (MQ=255)
                                           gggCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCAcgtc                                                                                                          >  1:198756/1‑149 (MQ=255)
                                               cAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTaa                                                                                                      <  1:912000/149‑1 (MQ=255)
                                                aaTCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAAc                                                                                                     <  1:1375112/149‑1 (MQ=255)
                                                  tCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCCTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCTCGGGCGCGGCCTTCCACGTCGTAACGc                                                                                                   >  1:680948/1‑149 (MQ=255)
                                                        tCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGcaccaca                                                                                             >  4:849849/1‑149 (MQ=255)
                                                        tCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGcaccaca                                                                                             >  1:1133712/1‑149 (MQ=255)
                                                        tCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGcaccaca                                                                                             >  1:560983/1‑149 (MQ=255)
                                                          aCCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGcaccacacc                                                                                           >  4:495825/1‑149 (MQ=255)
                                                          aCCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGcaccacacc                                                                                           >  3:1178788/1‑149 (MQ=255)
                                                           ccGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGcaccacacca                                                                                          >  2:1411224/1‑149 (MQ=255)
                                                            cGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCaa                                                                                         >  3:719103/1‑149 (MQ=255)
                                                                gTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGt                                                                                     <  2:430584/149‑1 (MQ=255)
                                                                gTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGt                                                                                     <  2:110925/149‑1 (MQ=255)
                                                                  ttCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGa                                                                                   <  2:659307/149‑1 (MQ=255)
                                                                  ttCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGa                                                                                   >  2:457151/1‑149 (MQ=255)
                                                                     tGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCAACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACgt                                                                                >  2:294572/1‑149 (MQ=255)
                                                                      ggATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACgtg                                                                               <  4:719103/149‑1 (MQ=255)
                                                                      ggATTGGCTGACAGGCGGAAGTTATCGTTATGTTCGATAAAGACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCGCCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCAAACCAACGGTGACgtg                                                                               <  4:647342/149‑1 (MQ=255)
                                                                       gATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACgtgt                                                                              >  1:356346/1‑149 (MQ=255)
                                                                         ttGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTAc                                                                            <  2:1458271/149‑1 (MQ=255)
                                                                             ctggcAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCg                                                                        >  2:916135/1‑149 (MQ=255)
                                                                                  aGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTtctc                                                                   >  1:750810/1‑149 (MQ=255)
                                                                                  aGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTtctc                                                                   >  1:824992/1‑149 (MQ=255)
                                                                                    gCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGa                                                                 >  4:1160549/1‑149 (MQ=255)
                                                                                     cGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGAc                                                                <  3:1453248/149‑1 (MQ=255)
                                                                                      ggAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACt                                                               >  1:711882/1‑149 (MQ=255)
                                                                                       gAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACtt                                                              >  4:819153/1‑149 (MQ=255)
                                                                                         aGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTGCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTcc                                                            <  2:1229911/149‑1 (MQ=255)
                                                                                           ttATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGc                                                          <  2:110197/149‑1 (MQ=255)
                                                                                              tCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACgtg                                                                               >  1:933582/1‑125 (MQ=255)
                                                                                               cGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGc                                                      <  2:9846/149‑1 (MQ=255)
                                                                                                 ttATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCga                                                    >  4:1242807/1‑149 (MQ=255)
                                                                                                 ttATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCga                                                    >  3:936495/1‑149 (MQ=255)
                                                                                                     gTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTg                                                                                    <  2:867516/113‑1 (MQ=255)
                                                                                                     gTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTg                                                                                    >  1:867516/1‑113 (MQ=255)
                                                                                                     gTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCgaggag                                                <  3:911270/149‑1 (MQ=255)
                                                                                                      ttCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGaggagg                                               >  1:1243902/1‑149 (MQ=255)
                                                                                                         gATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGa                                            <  3:1160549/149‑1 (MQ=255)
                                                                                                          aTAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGAt                                           >  1:1454617/1‑149 (MQ=255)
                                                                                                            aaaTACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATgg                                         <  1:242741/149‑1 (MQ=255)
                                                                                                             aaTACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGa                                        >  1:130120/1‑149 (MQ=255)
                                                                                                              aTACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGaa                                       >  1:335138/1‑149 (MQ=255)
                                                                                                              aTACGCGGACTTCGCCCTATTCTTCCACGCGGTCAGTGAGGAAGCTGGAGGCACCACAGGCACGGGCGCGCCATGCCACGTCGTAATGCCCCACACCAACGGAGGCTTGTACTACTTTCTCGCCGTCCGCCTGCGAGAAGGCGATGGaa                                       <  2:1287199/149‑1 (MQ=255)
                                                                                                               tACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAAt                                      >  2:483699/1‑149 (MQ=255)
                                                                                                                aCGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATa                                     <  2:391490/149‑1 (MQ=255)
                                                                                                                aCGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCCACTTCCGCCTGCGAGGAGGCGATGGAATa                                     <  2:492314/149‑1 (MQ=255)
                                                                                                                     gACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGAc                                <  3:1313671/149‑1 (MQ=255)
                                                                                                                      aCTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGAcg                               >  4:440997/1‑149 (MQ=255)
                                                                                                                        ttCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAAGGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGAcgcg                             >  2:1055151/1‑149 (MQ=255)
                                                                                                                        ttCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGAcgcg                             >  1:477313/1‑149 (MQ=255)
                                                                                                                        ttCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGAcgcg                             >  4:1430668/1‑149 (MQ=255)
                                                                                                                          cGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACgcggc                           >  2:1282197/1‑149 (MQ=255)
                                                                                                                          cGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACgcggc                           <  3:147794/149‑1 (MQ=255)
                                                                                                                           gCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACgcggcg                          >  1:248480/1‑149 (MQ=255)
                                                                                                                             ccTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGa                                            >  3:78342/1‑129 (MQ=255)
                                                                                                                             ccTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGa                                            <  4:78342/129‑1 (MQ=255)
                                                                                                                             ccTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTc                        >  2:839186/1‑149 (MQ=255)
                                                                                                                              ctctTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACgcggc                           <  3:599135/145‑1 (MQ=255)
                                                                                                                              ctctTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACgcggc                           >  4:599135/1‑145 (MQ=255)
                                                                                                                               tcttctTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGAt                                           <  3:424776/128‑1 (MQ=255)
                                                                                                                               tcttctTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGAt                                           >  4:424776/1‑128 (MQ=255)
                                                                                                                                cttcttCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGc                     >  2:919957/1‑149 (MQ=255)
                                                                                                                                  gcttcCTCGCTGACCGCGTGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCgg                   >  2:133031/2‑149 (MQ=255)
                                                                                                                                    ttcCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGCCTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGgc                 >  2:365326/1‑149 (MQ=255)
                                                                                                                                    ttcCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGAc                                <  1:1360837/134‑1 (MQ=255)
                                                                                                                                    ttcCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGAc                                >  2:1360837/1‑134 (MQ=255)
                                                                                                                                    ttcCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGgc                 >  4:211087/1‑149 (MQ=255)
                                                                                                                                       cACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCgca              >  3:33120/1‑149 (MQ=255)
                                                                                                                                        aCGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCgg                   >  4:431046/1‑143 (MQ=255)
                                                                                                                                        aCGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCgg                   <  3:431046/143‑1 (MQ=255)
                                                                                                                                        aCGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCgcag             <  3:825858/149‑1 (MQ=255)
                                                                                                                                            ggTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGa         <  4:598402/149‑1 (MQ=255)
                                                                                                                                            ggTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGa         <  1:836402/149‑1 (MQ=255)
                                                                                                                                                aGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGattaa     <  2:711882/149‑1 (MQ=255)
                                                                                                                                                 gCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGattaat    <  2:1482746/149‑1 (MQ=255)
                                                                                                                                                 gCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGattaat    >  1:872522/1‑149 (MQ=255)
                                                                                                                                                  cGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGattaatt   >  4:755408/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCGTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTA  >  NC_000913/2890477‑2890772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: