Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,939,331 A→G 5.2% intergenic (+21/‑37) fucU → / → fucR L‑fucose mutarotase/l‑fucose operon activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,939,3310AG 5.2% 519.0 / 12.2 155intergenic (+21/‑37)fucU/fucRL‑fucose mutarotase/l‑fucose operon activator
Reads supporting (aligned to +/- strand):  ref base A (69/78);  new base G (5/3);  total (74/81)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.82e-01

Only 100 of 155 total aligned reads displayed.

CTGACATCATCCGCATCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTCGC  >  NC_000913/2939186‑2939478
                                                                                                                                                 |                                                                                                                                                   
cTGACATCATCCGCATCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGa                                                                                                                                                  >  3:248996/1‑149 (MQ=255)
 tGACATCATCCGCATCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGAc                                                                                                                                                 <  3:563214/149‑1 (MQ=255)
 tGACATCATCCGCATCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGAc                                                                                                                                                 >  1:1175416/1‑149 (MQ=255)
 tGACATCATCCGCATCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGAc                                                                                                                                                 >  1:534226/1‑149 (MQ=255)
     atcatcCGCATCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACgcgg                                                                                                                                             >  2:608058/1‑149 (MQ=255)
        atcCGCATCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACgcgggcg                                                                                                                                          <  2:208661/149‑1 (MQ=255)
          ccGCATCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCGTCATgcgggcgta                                                                                                                                        <  1:890258/149‑3 (MQ=255)
              aTCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATc                                                                                                                                    <  1:1344229/149‑1 (MQ=255)
              aTCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATc                                                                                                                                    >  2:165231/1‑149 (MQ=255)
               tCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCg                                                                                                                                   >  1:1456894/1‑149 (MQ=255)
                cAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGa                                                                                                                                  <  4:1282244/149‑1 (MQ=255)
                  aTCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAat                                                                                                                                >  3:384037/1‑149 (MQ=255)
                  aTCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAat                                                                                                                                >  3:11864/1‑149 (MQ=255)
                  aTCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAat                                                                                                                                >  2:408206/1‑149 (MQ=255)
                      ttttGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAatgatg                                                                                                                            <  1:408206/149‑1 (MQ=255)
                        ttGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGCAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATggg                                                                                                                          <  1:467683/149‑1 (MQ=255)
                        ttGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATggg                                                                                                                          <  2:794486/149‑1 (MQ=255)
                        ttGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATggg                                                                                                                          <  1:947660/149‑1 (MQ=255)
                         tGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATgggg                                                                                                                         <  3:24704/149‑1 (MQ=255)
                          gCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGt                                                                                                                        <  4:613412/149‑1 (MQ=255)
                            gTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGa                                                                                                                      <  3:967030/149‑1 (MQ=255)
                            gTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGa                                                                                                                      >  4:1323595/1‑149 (MQ=255)
                                 aTGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAat                                                                                                                 <  3:95437/149‑1 (MQ=255)
                                  tGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAata                                                                                                                <  4:854495/149‑1 (MQ=255)
                                  tGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAata                                                                                                                <  1:1106387/149‑1 (MQ=255)
                                   gAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAGAatat                                                                                                               >  2:616032/1‑149 (MQ=255)
                                       gggCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGaaa                                                                                                           <  4:248996/149‑1 (MQ=255)
                                       gggCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGaaa                                                                                                           >  2:240321/1‑149 (MQ=255)
                                         gcgcAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGc                                                                                                         >  4:463779/1‑149 (MQ=255)
                                          cgcAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCg                                                                                                        <  1:1399679/149‑1 (MQ=255)
                                               aaaGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACg                                                                                                   >  1:1026189/1‑149 (MQ=255)
                                                     tttGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATgggg                                                                                                                         <  2:357387/121‑1 (MQ=255)
                                                     tttGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCa                                                                                             >  4:1232586/1‑149 (MQ=255)
                                                      ttGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCaa                                                                                            <  2:1026189/149‑1 (MQ=255)
                                                      ttGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCaa                                                                                            <  3:689200/149‑1 (MQ=255)
                                                          taTCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCACATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGa                                                                                        >  3:1116494/2‑149 (MQ=255)
                                                          gATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGa                                                                                        <  2:956312/149‑1 (MQ=255)
                                                            tCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATa                                                                                      <  1:1376722/149‑1 (MQ=255)
                                                            tCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATa                                                                                      <  1:465419/149‑1 (MQ=255)
                                                            tCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATa                                                                                      <  4:11864/149‑1 (MQ=255)
                                                            tCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATa                                                                                      <  4:1244230/149‑1 (MQ=255)
                                                            tCGGTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACACGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGAGGCACGCCAGCAAGCGATa                                                                                      >  2:1234994/1‑149 (MQ=255)
                                                                tATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCg                                                                                  <  4:814217/149‑1 (MQ=255)
                                                                   cacaGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGAcc                                                                               >  3:766438/1‑149 (MQ=255)
                                                                    acaGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACct                                                                              >  2:310645/1‑149 (MQ=255)
                                                                    acaGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACct                                                                              >  2:1169375/1‑149 (MQ=255)
                                                                    acaGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACct                                                                              >  2:1445282/1‑149 (MQ=255)
                                                                       ggCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGAc                                                                                <  3:933186/144‑1 (MQ=255)
                                                                       ggCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACctgct                                                                           <  4:678485/149‑1 (MQ=255)
                                                                       ggCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGATAACGAATGGCGGGGTGAAAAATATGAAAACGGCACGCCAGAAAGCGAGAGTCGACctgct                                                                           >  2:95775/1‑149 (MQ=255)
                                                                          gAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCGTCATgcgggcgta                                                                                                                                        >  2:1152758/1‑83 (MQ=255)
                                                                             cGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGaa                                                                     <  1:1166553/149‑1 (MQ=255)
                                                                              gAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAGAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAAc                                                                    <  1:616032/149‑1 (MQ=255)
                                                                              gAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAAc                                                                    <  3:463779/149‑1 (MQ=255)
                                                                                   aaGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATAc                                                               <  3:1232586/149‑1 (MQ=255)
                                                                                    aGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCg                                                                                                        <  4:1348521/107‑1 (MQ=255)
                                                                                    aGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCg                                                                                                        >  4:793520/1‑107 (MQ=255)
                                                                                    aGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCg                                                                                                        >  3:1348521/1‑107 (MQ=255)
                                                                                    aGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCg                                                                                                        <  3:793520/107‑1 (MQ=255)
                                                                                        cGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGcc                                                          >  3:820719/1‑149 (MQ=255)
                                                                                        cGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGcc                                                          <  2:534226/149‑1 (MQ=255)
                                                                                         gggAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCt                                                         <  2:906659/149‑1 (MQ=255)
                                                                                          ggAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTg                                                        >  2:670700/1‑149 (MQ=255)
                                                                                           gAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGa                                                       >  2:788816/1‑149 (MQ=255)
                                                                                                ttCTTTTAAAAAAGGGGTGAACACCTTAATCTCATCCCGGTACGCCCCCATTACGCGGGCGGTTATCGAAATTTGGGTCGAAAAATATGAAATCGGCACGCCAGCAAGCGATAGTCGACCTGCTGTTCAACCATACAAGCCTGCCCACg                                                  >  2:740065/1‑149 (MQ=255)
                                                                                                   ttttAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGaa                                               <  3:187324/149‑1 (MQ=255)
                                                                                                   ttttAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGaa                                               >  4:1284299/1‑149 (MQ=255)
                                                                                                   ttttAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGaa                                               <  1:1234994/149‑1 (MQ=255)
                                                                                                   ttttAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGaa                                               >  4:374535/1‑149 (MQ=255)
                                                                                                   ttttAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCAACCTGCTGCTGAACCATACCAGCCTGACCACGGaa                                               >  4:950788/1‑149 (MQ=255)
                                                                                                    tttAAAAAAAGGGGTAACACCGTAATCTCATACCGGTAGGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAg                                              <  4:115454/149‑1 (MQ=255)
                                                                                                    tttAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAg                                                                                                          <  4:614437/89‑1 (MQ=255)
                                                                                                    tttAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAg                                                                                                          >  3:614437/1‑89 (MQ=255)
                                                                                                       aaaaaaaGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGctc                                           >  3:559574/1‑149 (MQ=255)
                                                                                                        aaaaaaGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGGGAAAAATATGAAAGCGGCACGCCAGAACGCGATAGTCGACCTGCTGCTGAACCATACAAGCCTGACGCCGGAAGctct                                          >  3:334531/1‑149 (MQ=255)
                                                                                                         aaaaaGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGc                                                                                                         >  1:399949/1‑85 (MQ=255)
                                                                                                         aaaaaGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGc                                                                                                         <  2:399949/85‑1 (MQ=255)
                                                                                                          aaaaGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGctctct                                        <  4:766438/149‑1 (MQ=255)
                                                                                                           aaaGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCa                                                                  <  1:859949/122‑1 (MQ=255)
                                                                                                           aaaGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCa                                                                  >  2:859949/1‑122 (MQ=255)
                                                                                                           aaaGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATa                                                                >  1:1311115/1‑124 (MQ=255)
                                                                                                           aaaGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATa                                                                <  2:1311115/124‑1 (MQ=255)
                                                                                                                  tAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAg                                <  2:1092438/149‑1 (MQ=255)
                                                                                                                      acCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATCCCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTaa                            <  1:851054/149‑1 (MQ=255)
                                                                                                                       ccGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTaaa                           <  2:572922/149‑1 (MQ=255)
                                                                                                                        cGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGctctctc                                       <  1:648884/136‑1 (MQ=255)
                                                                                                                          tAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGt                        >  2:1017537/1‑149 (MQ=255)
                                                                                                                          tAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGt                        <  1:165231/149‑1 (MQ=255)
                                                                                                                            aTCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCa                      <  1:1293449/149‑1 (MQ=255)
                                                                                                                               tcATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGACGct                                            <  3:1135858/124‑1 (MQ=255)
                                                                                                                               tcATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTa                   >  1:786794/1‑149 (MQ=255)
                                                                                                                                 aTACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTaaa                 >  2:284/1‑149 (MQ=255)
                                                                                                                                     cGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGaaa             <  3:931681/149‑1 (MQ=255)
                                                                                                                                      taaccGCCCGCGTCATGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAAc            >  1:184144/5‑149 (MQ=255)
                                                                                                                                      taaccGCCCGCGTCATGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAAc            >  3:462194/5‑149 (MQ=255)
                                                                                                                                      ggTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAAc            >  3:1207091/1‑149 (MQ=255)
                                                                                                                                         ccGCCCGCGTCATGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCAt         >  2:964237/2‑149 (MQ=255)
                                                                                                                                         ccGCCCGCGTCATGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCAt         >  4:1086931/2‑149 (MQ=255)
                                                                                                                                         aCGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCAt         >  1:774143/1‑149 (MQ=255)
                                                                                                                                             ccGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCATGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATtcgt     >  4:892553/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CTGACATCATCCGCATCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTCGC  >  NC_000913/2939186‑2939478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: