Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,939,335 C→T 5.3% intergenic (+25/‑33) fucU → / → fucR L‑fucose mutarotase/l‑fucose operon activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,939,3350CT 5.3% 508.0 / 12.3 151intergenic (+25/‑33)fucU/fucRL‑fucose mutarotase/l‑fucose operon activator
Reads supporting (aligned to +/- strand):  ref base C (66/77);  new base T (5/3);  total (71/80)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.46e-01

Only 100 of 156 total aligned reads displayed.

TGACATCATCCGCATCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTCGCGATC  >  NC_000913/2939187‑2939482
                                                                                                                                                    |                                                                                                                                                   
tGACATCATCCGCATCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGAc                                                                                                                                                     <  3:563214/149‑1 (MQ=255)
    atcatcCGCATCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACgcgg                                                                                                                                                 >  2:608058/1‑149 (MQ=255)
       atcCGCATCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACgcgggcg                                                                                                                                              <  2:208661/149‑1 (MQ=255)
       atcCGCATCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACgcgggcg                                                                                                                                              >  1:843525/1‑149 (MQ=255)
             aTCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATc                                                                                                                                        >  2:165231/1‑149 (MQ=255)
               cAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGa                                                                                                                                      <  4:1282244/149‑1 (MQ=255)
                aaTCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGaa                                                                                                                                     >  4:54400/1‑149 (MQ=255)
                 aTCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAat                                                                                                                                    >  2:408206/1‑149 (MQ=255)
                 aTCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAat                                                                                                                                    >  3:11864/1‑149 (MQ=255)
                 aTCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAat                                                                                                                                    >  3:320978/1‑149 (MQ=255)
                 aTCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAat                                                                                                                                    >  3:384037/1‑149 (MQ=255)
                   cGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAatga                                                                                                                                  <  1:465019/149‑1 (MQ=255)
                     ttttGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGtt                                                                                                                                           <  2:1183918/138‑1 (MQ=255)
                     ttttGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGtt                                                                                                                                           >  1:1183918/1‑138 (MQ=255)
                     ttttGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAatgatg                                                                                                                                <  2:384173/149‑1 (MQ=255)
                       ttGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATggg                                                                                                                              <  2:1159381/149‑1 (MQ=255)
                       ttGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATggg                                                                                                                              <  1:947660/149‑1 (MQ=255)
                        tGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATgggg                                                                                                                             <  3:24704/149‑1 (MQ=255)
                           gTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGa                                                                                                                          <  3:967030/149‑1 (MQ=255)
                           gTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGa                                                                                                                          >  4:1323595/1‑149 (MQ=255)
                                aTGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAat                                                                                                                     <  3:95437/149‑1 (MQ=255)
                                 tGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAata                                                                                                                    <  4:854495/149‑1 (MQ=255)
                                 tGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAata                                                                                                                    <  1:1106387/149‑1 (MQ=255)
                                  gAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAGAatat                                                                                                                   >  2:616032/1‑149 (MQ=255)
                                  gAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAatat                                                                                                                   <  2:810121/149‑1 (MQ=255)
                                  gAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAatat                                                                                                                   <  1:617458/149‑1 (MQ=255)
                                   aaCGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATg                                                                                                                  >  4:1202850/1‑149 (MQ=255)
                                      gggCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGaaa                                                                                                               <  4:248996/149‑1 (MQ=255)
                                      gggCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGaaa                                                                                                               >  2:240321/1‑149 (MQ=255)
                                        gcgcAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGc                                                                                                             >  4:463779/1‑149 (MQ=255)
                                         cgcAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCg                                                                                                            <  1:1399679/149‑1 (MQ=255)
                                              aaaGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACg                                                                                                       >  1:1026189/1‑149 (MQ=255)
                                               aaGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGCGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGc                                                                                                      >  1:1476283/1‑149 (MQ=255)
                                                  ccTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAg                                                                                                   >  1:956312/1‑149 (MQ=255)
                                                    tttGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATgggg                                                                                                                             >  1:357387/1‑121 (MQ=255)
                                                    tttGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATgggg                                                                                                                             <  2:357387/121‑1 (MQ=255)
                                                     ttGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCaa                                                                                                <  2:1026189/149‑1 (MQ=255)
                                                         taTCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCACATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGa                                                                                            >  3:1116494/2‑149 (MQ=255)
                                                         gATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGa                                                                                            <  2:956312/149‑1 (MQ=255)
                                                           tCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATa                                                                                          <  4:1244230/149‑1 (MQ=255)
                                                           tCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATa                                                                                          <  4:11864/149‑1 (MQ=255)
                                                           tCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATa                                                                                          <  1:465419/149‑1 (MQ=255)
                                                           tCGGTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACACGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGAGGCACGCCAGCAAGCGATa                                                                                          >  2:1234994/1‑149 (MQ=255)
                                                               tATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCg                                                                                      <  4:814217/149‑1 (MQ=255)
                                                                  cacaGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGAcc                                                                                   >  3:766438/1‑149 (MQ=255)
                                                                   acaGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACct                                                                                  >  2:310645/1‑149 (MQ=255)
                                                                   acaGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACct                                                                                  >  2:1445282/1‑149 (MQ=255)
                                                                      ggCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGAc                                                                                    <  3:933186/144‑1 (MQ=255)
                                                                      ggCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACctgct                                                                               <  1:1370837/149‑1 (MQ=255)
                                                                      ggCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACctgct                                                                               <  4:678485/149‑1 (MQ=255)
                                                                      ggCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGATAACGAATGGCGGGGTGAAAAATATGAAAACGGCACGCCAGAAAGCGAGAGTCGACctgct                                                                               >  2:95775/1‑149 (MQ=255)
                                                                         gAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCGTCATgcgggcgta                                                                                                                                            <  1:1152758/85‑3 (MQ=255)
                                                                         gAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCGTCATgcgggcgta                                                                                                                                            >  2:1152758/1‑83 (MQ=255)
                                                                            cGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGaa                                                                         <  1:1166553/149‑1 (MQ=255)
                                                                             gAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAGAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAAc                                                                        <  1:616032/149‑1 (MQ=255)
                                                                             gAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAAc                                                                        <  3:463779/149‑1 (MQ=255)
                                                                               gCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCa                                                                      >  1:868127/1‑149 (MQ=255)
                                                                                  aaGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATAc                                                                   <  3:1232586/149‑1 (MQ=255)
                                                                                   aGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCg                                                                                                            <  4:1348521/107‑1 (MQ=255)
                                                                                   aGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCg                                                                                                            >  4:793520/1‑107 (MQ=255)
                                                                                   aGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCg                                                                                                            >  3:1348521/1‑107 (MQ=255)
                                                                                       cGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGcc                                                              <  2:534226/149‑1 (MQ=255)
                                                                                       cGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGcc                                                              >  3:820719/1‑149 (MQ=255)
                                                                                        gggAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCt                                                             <  2:906659/149‑1 (MQ=255)
                                                                                         ggAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTg                                                            >  2:670700/1‑149 (MQ=255)
                                                                                          gAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGa                                                           >  2:788816/1‑149 (MQ=255)
                                                                                               ttCTTTTAAAAAAGGGGTGAACACCTTAATCTCATCCCGGTACGCCCCCATTACGCGGGCGGTTATCGAAATTTGGGTCGAAAAATATGAAATCGGCACGCCAGCAAGCGATAGTCGACCTGCTGTTCAACCATACAAGCCTGCCCACg                                                      >  2:740065/1‑149 (MQ=255)
                                                                                               ttCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACg                                                      <  1:1298694/149‑1 (MQ=255)
                                                                                                  ttttAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGaa                                                   >  4:1284299/1‑149 (MQ=255)
                                                                                                  ttttAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGaa                                                   <  1:1234994/149‑1 (MQ=255)
                                                                                                  ttttAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGaa                                                   >  4:374535/1‑149 (MQ=255)
                                                                                                  ttttAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGaa                                                   <  3:187324/149‑1 (MQ=255)
                                                                                                  ttttAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGaa                                                   >  3:665273/1‑149 (MQ=255)
                                                                                                  ttttAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCAACCTGCTGCTGAACCATACCAGCCTGACCACGGaa                                                   >  4:950788/1‑149 (MQ=255)
                                                                                                   tttAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAg                                                                                                              >  3:614437/1‑89 (MQ=255)
                                                                                                   tttAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAg                                                                                                              <  4:614437/89‑1 (MQ=255)
                                                                                                      aaaaaaaGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGctc                                               >  3:559574/1‑149 (MQ=255)
                                                                                                        aaaaaGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGc                                                                                                             >  1:399949/1‑85 (MQ=255)
                                                                                                         aaaaGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGctctct                                            <  3:776951/149‑1 (MQ=255)
                                                                                                         aaaaGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGctctct                                            <  4:766438/149‑1 (MQ=255)
                                                                                                         aaaaGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGctctct                                            <  1:1456451/149‑1 (MQ=255)
                                                                                                          aaaGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCa                                                                      >  2:859949/1‑122 (MQ=255)
                                                                                                          aaaGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATa                                                                    >  1:1311115/1‑124 (MQ=255)
                                                                                                          aaaGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATa                                                                    <  2:1311115/124‑1 (MQ=255)
                                                                                                               ggTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAAc                                      <  1:765115/149‑1 (MQ=255)
                                                                                                                  aaCACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGc                                   <  2:1059085/149‑1 (MQ=255)
                                                                                                                  aaCACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGc                                   >  3:401063/1‑149 (MQ=255)
                                                                                                                    cacCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTa                                 <  1:474503/149‑1 (MQ=255)
                                                                                                                     acCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATCCCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTaa                                <  1:851054/149‑1 (MQ=255)
                                                                                                                      ccGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTaaa                               <  2:572922/149‑1 (MQ=255)
                                                                                                                         tAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGt                            <  1:165231/149‑1 (MQ=255)
                                                                                                                              tcATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGACGct                                                <  3:1135858/124‑1 (MQ=255)
                                                                                                                              tcATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGct                                                >  4:1135858/1‑124 (MQ=255)
                                                                                                                                aTACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTaaa                     >  2:284/1‑149 (MQ=255)
                                                                                                                                    cGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGaaa                 <  3:931681/149‑1 (MQ=255)
                                                                                                                                        ccGCCCGCGTCATGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCAt             >  4:1086931/2‑149 (MQ=255)
                                                                                                                                        ccGCCCGCGTCATGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCAt             >  2:964237/2‑149 (MQ=255)
                                                                                                                                        aCGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCAt             >  1:774143/1‑149 (MQ=255)
                                                                                                                                            ccGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATtcgt         >  3:1217606/1‑149 (MQ=255)
                                                                                                                                            ccGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCATGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATtcgt         >  4:892553/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGACATCATCCGCATCAATCGTTTTGCGTTTTATGAACGGGCGCAAAAAGCCTTTGCGATCGTTATCACAGGCGAACGAGCGAAGTACGGGAATATTCTTTTAAAAAAAGGGGTAACACCGTAATCTCATACCGGTACGCCCGCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTCGCGATC  >  NC_000913/2939187‑2939482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: