Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,972,652 A→T 9.9% intergenic (+15/+17) tas → / ← lplT putative NADP(H)‑dependent aldo‑keto reductase/lysophospholipid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,972,6520AT 9.9% 402.7 / 28.8 123intergenic (+15/+17)tas/lplTputative NADP(H)‑dependent aldo‑keto reductase/lysophospholipid transporter
Reads supporting (aligned to +/- strand):  ref base A (57/52);  new base T (8/4);  total (66/57)
Fisher's exact test for biased strand distribution p-value = 3.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.21e-01

Only 100 of 126 total aligned reads displayed.

CCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACAT  >  NC_000913/2972504‑2972800
                                                                                                                                                    |                                                                                                                                                             
       tctGCTGGGCGCAACCACGATGGATCAGCCGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCgg                                                                                                                                                        <  3:608123/149‑1 (MQ=255)
          gctgGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTTTAACCGGCGt                                                                                                                                                     <  1:1079563/149‑1 (MQ=255)
          gctgGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTTTAACCGGCGt                                                                                                                                                     <  3:1102554/149‑1 (MQ=255)
           ctgGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTTTAACCGGCGtt                                                                                                                                                    <  2:1105491/149‑1 (MQ=255)
                cgcAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAata                                                                                                                                               <  3:818415/149‑1 (MQ=255)
                 gcAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAatat                                                                                                                                              <  2:790407/149‑1 (MQ=255)
                   aaCCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTa                                                                                                                                            >  1:44656/1‑149 (MQ=255)
                   aaCCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTa                                                                                                                                            >  2:1390511/1‑149 (MQ=255)
                     ccACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAAt                                                                                                                                          <  2:44656/149‑1 (MQ=255)
                     ccACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAAt                                                                                                                                          >  2:386221/1‑149 (MQ=255)
                      cACGATGGATCGGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAata                                                                                                                                               >  3:827774/1‑143 (MQ=255)
                      cACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAata                                                                                                                                               <  4:827774/143‑1 (MQ=255)
                         gATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAAt                                                                                                                                          >  4:1089820/1‑145 (MQ=255)
                         gATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAAt                                                                                                                                          <  3:1089820/145‑1 (MQ=255)
                         gATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACg                                                                                                                                      <  3:1444891/149‑1 (MQ=255)
                              aTCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCt                                                                                                                                 <  1:1342280/149‑1 (MQ=255)
                              aTCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCAGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCt                                                                                                                                 >  4:283839/1‑149 (MQ=255)
                                cAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGc                                                                                                                               <  3:773136/149‑1 (MQ=255)
                                    tGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAat                                                                                                                                                >  1:492882/1‑128 (MQ=255)
                                    tGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGa                                                                                                                           >  1:1317457/1‑149 (MQ=255)
                                     gAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGAt                                                                                                                          <  1:386221/149‑1 (MQ=255)
                                        aaCTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCca                                                                                                                       >  1:1100434/1‑149 (MQ=255)
                                           tAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAg                                                                                                                    >  2:650191/1‑149 (MQ=255)
                                           tAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAg                                                                                                                    >  4:390480/1‑149 (MQ=255)
                                           tAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAg                                                                                                                    >  3:389602/1‑149 (MQ=255)
                                           tAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAg                                                                                                                    >  4:831610/1‑149 (MQ=255)
                                             aCATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAgcg                                                                                                                  >  1:228822/1‑149 (MQ=255)
                                                 cGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGt                                                                                                              <  2:428494/149‑1 (MQ=255)
                                                  gAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGtt                                                                                                             >  1:179367/1‑149 (MQ=255)
                                                  gAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGtt                                                                                                             >  2:704194/1‑149 (MQ=255)
                                                      gTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTg                                                                                                         >  4:218098/1‑149 (MQ=255)
                                                      gTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTg                                                                                                         <  2:553512/149‑1 (MQ=255)
                                                          gCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAg                                                                                            <  2:560217/149‑1 (MQ=255)
                                                             tCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAgcgc                                                                                         >  1:171324/1‑149 (MQ=255)
                                                             tCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCa                                                                                        >  3:1332225/1‑149 (MQ=255)
                                                                 gAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAAc                                                                                     <  2:1439423/149‑1 (MQ=255)
                                                                    ttAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAgc                                                                                  >  2:496645/1‑149 (MQ=255)
                                                                       aGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAata                                                                                                                                               <  3:386191/94‑1 (MQ=255)
                                                                        gCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCAc                                                                              >  1:293107/1‑149 (MQ=255)
                                                                                  aTTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAata                                                                                                                                               >  1:193274/1‑83 (MQ=255)
                                                                                  aTTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAata                                                                                                                                               <  2:193274/83‑1 (MQ=255)
                                                                                  aTTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAgccaat                                                                    <  3:390480/149‑1 (MQ=255)
                                                                                   ttAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAgccaatg                                                                   >  1:851247/1‑149 (MQ=255)
                                                                                   ttAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAgccaatg                                                                   >  4:1111653/1‑149 (MQ=255)
                                                                                       cTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAgccaatgccaa                                                               <  3:304062/149‑1 (MQ=255)
                                                                                        tGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAgccaatgccaat                                                              <  2:192287/149‑1 (MQ=255)
                                                                                        tGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAgccaatgccaat                                                              >  1:47902/1‑149 (MQ=255)
                                                                                        tGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAgccaatgccaat                                                              <  1:9742/149‑1 (MQ=255)
                                                                                        tGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAgccaatgccaat                                                              <  2:67165/149‑1 (MQ=255)
                                                                                        tGAAATTGAAGCGGTGCATCAGGTTTATACTTACCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAgccaatgccaat                                                              >  4:258020/1‑149 (MQ=255)
                                                                                        tGAAATTGAAGCGGTGCATCAGGTTTATACTAATCCGGCTCCAGAAGATTAACGCCGGTTAAGACCGGCGTTAAATCTAAATGACGACGCTGCCAGATCCACAGCGCTGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAgccaatgccaat                                                              >  3:1218671/1‑149 (MQ=255)
                                                                                              tGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCAc                                                        <  1:882725/149‑1 (MQ=255)
                                                                                                aaGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGa                                                      <  1:650191/149‑1 (MQ=255)
                                                                                                aaGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGa                                                      >  4:1157134/1‑149 (MQ=255)
                                                                                                 aGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGAc                                                     >  4:555866/1‑149 (MQ=255)
                                                                                                      tGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGAt                                                                                                                          >  3:449450/1‑84 (MQ=255)
                                                                                                      tGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGAt                                                                                                                          <  4:449450/84‑1 (MQ=255)
                                                                                                      tGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGa                                                <  2:1100434/149‑1 (MQ=255)
                                                                                                         aTCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAgccaatgc                                                                  <  1:1452766/128‑1 (MQ=255)
                                                                                                         aTCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAgccaatgc                                                                  >  2:1452766/1‑128 (MQ=255)
                                                                                                         aTCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGcc                                            <  4:1332225/149‑1 (MQ=255)
                                                                                                          tCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAgc                                                                                  >  2:1138506/1‑111 (MQ=255)
                                                                                                          tCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAgc                                                                                  <  1:1138506/111‑1 (MQ=255)
                                                                                                          tCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGcc                                            >  4:734299/1‑149 (MQ=255)
                                                                                                          tCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGcc                                            >  1:1288895/1‑149 (MQ=255)
                                                                                                            aGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACTGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTa                                          >  4:1302861/1‑149 (MQ=255)
                                                                                                             ggTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTAt                                         <  3:1111653/149‑1 (MQ=255)
                                                                                                              gTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTa                                          <  4:437507/147‑1 (MQ=255)
                                                                                                              gTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTa                                          >  3:437507/1‑147 (MQ=255)
                                                                                                               tttATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCa                                       <  3:311255/149‑1 (MQ=255)
                                                                                                                  ataCTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTa                                    <  2:228822/149‑1 (MQ=255)
                                                                                                                   taCTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGc                                             <  4:190477/139‑1 (MQ=255)
                                                                                                                   taCTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGc                                             >  3:190477/1‑139 (MQ=255)
                                                                                                                     cTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCg                                 >  4:471116/1‑149 (MQ=255)
                                                                                                                     cTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCg                                 >  3:1023356/1‑149 (MQ=255)
                                                                                                                            ggCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGa                          <  1:292269/149‑1 (MQ=255)
                                                                                                                               aCCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGATGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTa                       >  1:442865/1‑149 (MQ=255)
                                                                                                                                  aTAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAAt                    <  4:708851/149‑1 (MQ=255)
                                                                                                                                  aTAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAAt                    <  3:1157134/149‑1 (MQ=255)
                                                                                                                                   tAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATg                   <  3:258020/149‑1 (MQ=255)
                                                                                                                                    aaGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGc                  <  1:457264/149‑1 (MQ=255)
                                                                                                                                     tatttAACGCCGGTTTTAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGcc                 >  4:822510/4‑149 (MQ=255)
                                                                                                                                     aGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGcc                 <  1:409174/149‑1 (MQ=255)
                                                                                                                                       tttAACGCCGGTTTTAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCa               >  3:1416827/2‑149 (MQ=255)
                                                                                                                                       aTTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCa               <  3:462322/149‑1 (MQ=255)
                                                                                                                                       aTTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCa               >  1:618832/1‑149 (MQ=255)
                                                                                                                                         tAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGc             >  1:1056454/1‑149 (MQ=255)
                                                                                                                                           aCGCCGGTTTTAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCAt           >  2:1104322/1‑149 (MQ=255)
                                                                                                                                           aCGCCGGTTTTAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCAt           >  2:1071593/1‑149 (MQ=255)
                                                                                                                                           aCGCCGGTTTTAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCAt           >  1:218825/1‑149 (MQ=255)
                                                                                                                                           aCGCCGGTTTTAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCAt           >  1:86021/1‑149 (MQ=255)
                                                                                                                                           aCGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCAt           >  4:663107/1‑149 (MQ=255)
                                                                                                                                            cGCCGGTTTTAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATc          >  1:1313655/1‑149 (MQ=255)
                                                                                                                                              ccGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATcaa        <  2:47902/149‑1 (MQ=255)
                                                                                                                                              ccGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATcaa        >  1:782020/1‑149 (MQ=255)
                                                                                                                                               cGGTTTTAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATcaac       <  4:1118238/149‑1 (MQ=255)
                                                                                                                                               cGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATcaac       >  4:1294665/1‑149 (MQ=255)
                                                                                                                                                ggTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCa               <  2:1189493/149‑1 (MQ=255)
                                                                                                                                                 gTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATcaacaa     >  4:1319188/1‑149 (MQ=255)
                                                                                                                                                  ttAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCaacaac    <  1:704194/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                             
CCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGC‑‑‑‑‑‑‑‑‑CAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACAT  >  NC_000913/2972504‑2972800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: