Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,007,473 G→A 7.3% intergenic (+21/‑37) ygeW → / → ygeX putative carbamoyltransferase/2,3‑diaminopropionate ammonia lyase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,007,4730GA 7.3% 563.6 / 23.5 178intergenic (+21/‑37)ygeW/ygeXputative carbamoyltransferase/2,3‑diaminopropionate ammonia lyase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (85/79);  new base A (5/8);  total (91/87)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 179 total aligned reads displayed.

CGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCACCGCTCTTTTCGCAAAGCCAGGCCAAACTGGCGCGCCAGTTCCACCAGA  >  NC_000913/3007326‑3007621
                                                                                                                                                   |                                                                                                                                                    
cGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGt                                                                                                                                                     <  3:768403/149‑1 (MQ=255)
     cctacAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGGGAAATAACCCCTCGGGCCGATCAGCTGGTCGGcc                                                                                                                                                <  2:940177/149‑1 (MQ=255)
      ctacAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGACCAGCTGATCGGccc                                                                                                                                               <  1:285394/149‑1 (MQ=255)
       tacAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGACCAGCTGATCGGcccg                                                                                                                                              >  2:475922/1‑148 (MQ=255)
       tacAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGAGCCGATCAGCTGGTCGGCCct                                                                                                                                              >  2:827274/1‑149 (MQ=255)
        acAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGACCAGCTGATCGGcccga                                                                                                                                             <  1:1328143/149‑3 (MQ=255)
        acAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACACCTCGGGCCGATCAGCTGGTCGGCCctg                                                                                                                                             >  4:1256150/1‑148 (MQ=255)
          aaaGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAACTGGTCGGCCCTCTa                                                                                                                                           >  4:1122539/1‑149 (MQ=255)
          aaaGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGCAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTa                                                                                                                                           >  1:466412/1‑149 (MQ=255)
           aagaagCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGACCAGCTGATCGGcccgaggg                                                                                                                                          <  2:1349187/149‑6 (MQ=255)
           aagaagCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGACCAGCTGATCGGcccgaggg                                                                                                                                          <  1:475922/149‑6 (MQ=255)
            agaagCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTAtt                                                                                                                                         <  3:1059157/149‑1 (MQ=255)
             gaagCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTAttt                                                                                                                                        >  2:678464/1‑149 (MQ=255)
                gCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATTCGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCa                                                                                                                                     <  3:52583/149‑1 (MQ=255)
                  cAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCaga                                                                                                                                   <  3:415472/149‑1 (MQ=255)
                  cAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCaga                                                                                                                                   <  1:534378/149‑1 (MQ=255)
                   aggtGGAAGCCTTATATCATCGCCGCGATGATCCCGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGAAACTGCTGAAAGAAGCGCAAGAACGCGTGAAGTAACCCCTCGGGCCGATCAGCGGGTCGGCCCTCTATTTCCagat                                                                                                                                  <  2:102801/146‑2 (MQ=255)
                   aGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCagag                                                                                                                                  <  4:1086365/149‑1 (MQ=255)
                    gCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAgg                                                                                                                                 <  3:582031/149‑1 (MQ=255)
                     cTGGAAGACTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGc                                                                                                                                <  3:1256150/149‑1 (MQ=255)
                      tGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGcc                                                                                                                               <  1:22767/149‑1 (MQ=255)
                         aaGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCaaa                                                                                                                            >  4:517384/1‑149 (MQ=255)
                              ttATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggat                                                                                                                       >  2:518017/1‑149 (MQ=255)
                              ttATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggat                                                                                                                       >  1:382001/1‑149 (MQ=255)
                               tataTCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggata                                                                                                                      <  1:743404/149‑1 (MQ=255)
                               tataTCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggata                                                                                                                      <  3:593110/149‑1 (MQ=255)
                                atatCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggatag                                                                                                                     <  2:1062877/149‑1 (MQ=255)
                                  atcatcGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggatagga                                                                                                                   <  3:1122539/149‑1 (MQ=255)
                                     atcGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAGCTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTGCAGAGGCCAAAAGGATAGGatat                                                                                                                >  2:330122/1‑149 (MQ=255)
                                     atcGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGTTGGCCCCTCATTTCCCAAGGGCCAAAaggataggtatt                                                                                                                >  3:345692/1‑145 (MQ=255)
                                     atcGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGatat                                                                                                                >  2:1402121/1‑149 (MQ=255)
                                     atcGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGatat                                                                                                                <  1:1020219/149‑1 (MQ=255)
                                      tcGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATg                                                                                                               >  2:722421/1‑149 (MQ=255)
                                      tcGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATg                                                                                                               <  1:589768/149‑1 (MQ=255)
                                      tcGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGACCAGCTGATCGGc                                                                                                                                                 <  3:259799/115‑1 (MQ=255)
                                      tcGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGACCAGCTGATCGGc                                                                                                                                                 >  4:259799/1‑115 (MQ=255)
                                         ccgcGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTcc                                                                                                            >  4:775697/1‑149 (MQ=255)
                                           gcgATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGt                                                                                                          >  4:1055505/1‑149 (MQ=255)
                                             gatgatCCTGTCCCGTAAATACGCCAATCCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGttt                                                                                                        >  3:976298/1‑149 (MQ=255)
                                              atgatCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGtttt                                                                                                       <  4:623085/149‑1 (MQ=255)
                                                  tCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCa                                                                                                   <  1:685839/149‑1 (MQ=255)
                                                    cTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCAtt                                                                                                 <  1:722421/149‑1 (MQ=255)
                                                    cTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCAtt                                                                                                 <  1:884216/149‑1 (MQ=255)
                                                    cTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCAtt                                                                                                 >  2:189710/1‑149 (MQ=255)
                                                     tGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTg                                                                                                >  3:1441732/1‑149 (MQ=255)
                                                     tGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTg                                                                                                >  1:1217134/1‑149 (MQ=255)
                                                      gTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCct                                                                                                                                              >  1:203828/1‑102 (MQ=255)
                                                       tCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGaa                                                                                              >  4:321948/1‑149 (MQ=255)
                                                         ccGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCaaaa                                                                                                                           >  3:1084598/1‑118 (MQ=255)
                                                         ccGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCaaaa                                                                                                                           <  4:1084598/118‑1 (MQ=255)
                                                         ccGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTGCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGa                                                                                            >  3:929942/1‑149 (MQ=255)
                                                           gTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGAtt                                                                                          <  2:368416/149‑1 (MQ=255)
                                                            tAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTg                                                                                         >  3:372824/1‑149 (MQ=255)
                                                               aTACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGata                                                                                      <  1:991375/149‑1 (MQ=255)
                                                                  cGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCg                                                                                   <  3:321948/149‑1 (MQ=255)
                                                                      aaaCCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGa                                                                               <  2:1117718/149‑1 (MQ=255)
                                                                         ccAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATaa                                                                            <  2:1144968/149‑1 (MQ=255)
                                                                           aGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACa                                                                          >  4:865271/1‑149 (MQ=255)
                                                                              tGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAAt                                                                       <  3:622979/149‑1 (MQ=255)
                                                                                cACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAAttt                                                                     <  1:678464/149‑1 (MQ=255)
                                                                                 aCTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAAtttt                                                                    >  4:381137/1‑149 (MQ=255)
                                                                                  cTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAAttttt                                                                   <  1:1402121/149‑1 (MQ=255)
                                                                                  cTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAAttttt                                                                   >  1:172339/1‑149 (MQ=255)
                                                                                   tGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTc                                                                  >  2:1201093/1‑149 (MQ=255)
                                                                                        gAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGa                                                                               <  3:478222/131‑1 (MQ=255)
                                                                                        gAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGa                                                                               >  4:478222/1‑131 (MQ=255)
                                                                                          gCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCAt                                                                                                  <  4:587179/110‑1 (MQ=255)
                                                                                          gCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAAc                                                               >  3:162355/1‑145 (MQ=255)
                                                                                          gCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCg                                                           <  4:1441732/149‑1 (MQ=255)
                                                                                              ctgctgAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAAc                                                       <  3:491505/149‑1 (MQ=255)
                                                                                               tgctgAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggatag                                                                                                                     >  2:713373/1‑86 (MQ=255)
                                                                                               tgctgAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACa                                                      >  4:179340/1‑149 (MQ=255)
                                                                                                  tgAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCa                                                   >  2:555201/1‑149 (MQ=255)
                                                                                                  tgAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCa                                                   <  4:1176354/149‑1 (MQ=255)
                                                                                                   gAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCAc                                                  <  3:733504/149‑1 (MQ=255)
                                                                                                    aaaGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCAcc                                                 >  4:347265/1‑149 (MQ=255)
                                                                                                      agaagCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAAttttt                                                                   >  2:1310613/1‑129 (MQ=255)
                                                                                                      agaagCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAAttttt                                                                   <  1:1310613/129‑1 (MQ=255)
                                                                                                       gaagCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCACCGct                                              >  1:772317/1‑149 (MQ=255)
                                                                                                             cAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCACCGCTCTTTTc                                        <  2:740331/149‑1 (MQ=255)
                                                                                                             cAAGAACGCGCGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCACCGCTCTTTTc                                        <  4:929942/149‑1 (MQ=255)
                                                                                                                  aCGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCACCGCTCTTTTCGCaaa                                   <  2:1257991/149‑1 (MQ=255)
                                                                                                                  aCGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCACCGCTCTTTTCGCaaa                                   <  3:347265/149‑1 (MQ=255)
                                                                                                                        gAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCACCGCTCTTTTCGCAAAGCCAgg                             >  3:1365307/1‑149 (MQ=255)
                                                                                                                                cccTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCACCGCTCTTTTCGCAAAGCCAGGCCaaa                        <  1:1065140/146‑1 (MQ=255)
                                                                                                                                cccTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCACCGCTCTTTTCGCAAAGCCAGGCCaaa                        >  2:1065140/1‑146 (MQ=255)
                                                                                                                                 ccTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCACCGCTCTTTTCGCAAAGCCAGGCCAAACTgg                    >  3:406712/1‑149 (MQ=255)
                                                                                                                                  cTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCACCGCTCTTTTCGCAAAGCCAGGCCAAACTGgc                   >  1:509259/1‑149 (MQ=255)
                                                                                                                                  agagggCCGACCAGCTGATCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCACCGCTCTTTTCGCAAAGCCAGGCCAAACTgg                    <  2:345061/145‑1 (MQ=255)
                                                                                                                                     gggCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCACCGCTCTTTTCGCAAAGCCAGGCCAAACTGgcgcg                >  1:1360851/1‑149 (MQ=255)
                                                                                                                                      ggCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAag                                                                                                                          >  4:566983/1‑42 (MQ=255)
                                                                                                                                      ggCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAag                                                                                                                          <  3:566983/42‑1 (MQ=255)
                                                                                                                                       gCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCACCGCTCTTTTCGCAAAGCCAGGCCAAACTGGCGCGcc              >  3:117908/1‑149 (MQ=255)
                                                                                                                                       gCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCACCGCTCTTTTCGCAAAGCCAGGCCAAACTGGCGCGcc              >  2:41494/1‑149 (MQ=255)
                                                                                                                                            tCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGa                                                                                            >  4:412697/1‑66 (MQ=255)
                                                                                                                                            tCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATa                                                                             >  4:248474/1‑81 (MQ=255)
                                                                                                                                            tCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCACCGCTCTTTTCGCAAAGCCAGGCCAAACTGGCGCGCCAGTTc         >  1:1000952/1‑149 (MQ=255)
                                                                                                                                             cAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCACCGCTCTTTTCGCAAAGCCAGGCCAAACTGGCGCGCCAGtttg        <  1:827274/149‑3 (MQ=255)
                                                                                                                                                 tGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCACCGCTCTTTTCGCAAAGCCAGGCCAAACTGGCGCGCCAGTTccacca    >  1:320228/1‑149 (MQ=255)
                                                                                                                                                   gTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCACCGCTCTTTTCGCAAAGCCAGGCCAAACTGGCGCGCCAGTTCCACCAGa  >  4:718802/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCACCGCTCTTTTCGCAAAGCCAGGCCAAACTGGCGCGCCAGTTCCACCAGA  >  NC_000913/3007326‑3007621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: