Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,200,991 A→T 5.7% intergenic (‑26/‑216) ygiF ← / → ygiM inorganic triphosphatase/SH3 domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,200,9910AT 5.7% 619.7 / 18.5 176intergenic (‑26/‑216)ygiF/ygiMinorganic triphosphatase/SH3 domain protein
Reads supporting (aligned to +/- strand):  ref base A (86/80);  new base T (5/5);  total (91/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 183 total aligned reads displayed.

GTAAATATTCAGCAACTGCACGGGGTCATGGTGCTCGCCGCCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATGTCGA  >  NC_000913/3200846‑3201139
                                                                                                                                                 |                                                                                                                                                    
 taAATATTCAGCAACTGCACGGGGTCATGGTGCTCGCCGCCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAg                                                                                                                                                  >  4:399895/1‑149 (MQ=255)
 taAATATTCAGCAACTGCACGGGGTCATGGTGCTCGCCGCCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAg                                                                                                                                                  >  2:1418274/1‑149 (MQ=255)
 taAATATTCAGCAACTGCACGGGGTCATGGTGCTCGCCGCCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAg                                                                                                                                                  >  1:518297/1‑149 (MQ=255)
  aaaTATTCAGCAACTGCACGGGGTCATGGTGCTCGCCGCCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGt                                                                                                                                                 >  1:1286807/1‑149 (MQ=255)
    atatTCAGCAACTGCACGGGGTCATGGTGCTCGCCGCCCAGCGTATTGCGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGa                                                                                                                                               <  4:132656/149‑1 (MQ=255)
         cAGCAACTGCACGGGGTCATGGTGCTCGCCGCCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACTTATGTGAcataa                                                                                                                                          <  3:1240078/149‑5 (MQ=255)
          aGCAACTGCACGGGGTCATGGTGCTCGCCGCCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACTTATGTGAcataac                                                                                                                                         <  1:590002/149‑6 (MQ=255)
           gCAACTGCACGGGGTCATGGTGCTCGCCGCCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGaa                                                                                                                                        <  3:349202/149‑1 (MQ=255)
           gCAACTGCACGGGGTCATGGTGCTCGCCGCCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGaa                                                                                                                                        <  1:379049/149‑1 (MQ=255)
           gCAACTGCACGGGGTCATGGTGCTCGCCGCCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGaa                                                                                                                                        >  1:1058467/1‑149 (MQ=255)
           gCAACTGCACGGGGTCATGGTGCTCGCCGCCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGaa                                                                                                                                        >  4:1314636/1‑149 (MQ=255)
            cAACTGCACGGGGTCATGGTGCTCGCCGCCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAAc                                                                                                                                       >  4:1402251/1‑149 (MQ=255)
              aCTGCACGGGGTCATGGTGCTCGCCGCCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACgg                                                                                                                                     >  1:138256/1‑149 (MQ=255)
                      gggTCATGGTGCTCGCCGCCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTa                                                                                                                             >  3:968283/1‑149 (MQ=255)
                        gTCATGGTGCTCGCCGCCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAAt                                                                                                                           <  1:1057999/149‑1 (MQ=255)
                        gTCATGGTGCTCGCCGCCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTAACGGTTATGTCACATAAGTGCCGATGAACGGCGAACTTGAt                                                                                                                           <  4:1385822/149‑1 (MQ=255)
                           aTGGTGCTCGCCGCCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCg                                                                                                                        >  3:1395871/1‑149 (MQ=255)
                              gTGCTCGCCGCCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATc                                                                                                                     <  3:871920/149‑1 (MQ=255)
                                  tCGCCGCCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCtttt                                                                                                                 <  4:886070/149‑1 (MQ=255)
                                     ccgccCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTg                                                                                                              <  1:568241/149‑1 (MQ=255)
                                      cgccCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGt                                                                                                             <  4:324157/149‑1 (MQ=255)
                                        cccAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCa                                                                                                           >  3:1417674/1‑149 (MQ=255)
                                           aGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTa                                                                                                        <  2:41296/149‑1 (MQ=255)
                                            gCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAg                                                                                                       >  2:91105/1‑149 (MQ=255)
                                                    agaTGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTa                                                                                                        >  2:826205/1‑140 (MQ=255)
                                                    agaTGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTAtt                                                                                               >  3:1002154/1‑149 (MQ=255)
                                                      aTGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTCACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTAtttt                                                                                             >  2:1181567/1‑149 (MQ=255)
                                                        ggTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTgc                                                                                           >  4:1092533/1‑149 (MQ=255)
                                                          tCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTAACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTgcgc                                                                                         <  4:864281/149‑1 (MQ=255)
                                                             cGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTTAGCCATGTCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCtttttt                                                                                                               >  3:1444713/1‑124 (MQ=255)
                                                             cGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCtttttt                                                                                                               <  4:1444713/124‑1 (MQ=255)
                                                              gCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCtttt                                                                                                                 <  1:1163688/121‑1 (MQ=255)
                                                              gCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCaaa                                                                                     >  3:1355433/1‑149 (MQ=255)
                                                              gCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCaaa                                                                                     <  3:1295858/149‑1 (MQ=255)
                                                                 aCGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTg                                                                                  >  2:289653/1‑149 (MQ=255)
                                                                     cTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCAt                                                                              >  4:785665/1‑149 (MQ=255)
                                                                             cACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACggg                                                                      >  1:1336061/1‑149 (MQ=255)
                                                                               cTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCa                                                                    >  3:392395/1‑149 (MQ=255)
                                                                                tgtgATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCaa                                                                   >  2:1172474/1‑149 (MQ=255)
                                                                                    aTTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTg                                                               >  3:791168/1‑149 (MQ=255)
                                                                                     ttAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGa                                                              <  2:343118/149‑1 (MQ=255)
                                                                                     ttAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGACGAACTTAATGAGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGa                                                              >  2:1053545/1‑149 (MQ=255)
                                                                                      tAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGAc                                                             <  2:1185930/149‑1 (MQ=255)
                                                                                        aCAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACgg                                                           <  1:568836/149‑1 (MQ=255)
                                                                                        aCAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACgg                                                           >  2:989329/1‑149 (MQ=255)
                                                                                          aaTAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAg                                                                                                   >  1:507465/1‑107 (MQ=255)
                                                                                          aaTAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAg                                                                                                   <  2:507465/107‑1 (MQ=255)
                                                                                          aaTAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGc                                                         >  4:485367/1‑149 (MQ=255)
                                                                                           aTAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATa                                                                                                    <  3:587346/105‑1 (MQ=255)
                                                                                           aTAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCg                                                        >  4:1250651/1‑149 (MQ=255)
                                                                                                cTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTaaaa                                                   <  3:1250651/149‑1 (MQ=255)
                                                                                                 tttAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAg                                                  >  3:748719/1‑149 (MQ=255)
                                                                                                 tttAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAg                                                  >  4:406764/1‑149 (MQ=255)
                                                                                                 tttAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAg                                                  >  1:841782/1‑149 (MQ=255)
                                                                                                 tttAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAg                                                  >  4:1359172/1‑149 (MQ=255)
                                                                                                    aaTTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGttt                                               <  2:548304/149‑1 (MQ=255)
                                                                                                     aTTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTg                                              >  1:786141/1‑149 (MQ=255)
                                                                                                      ttCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACgg                                                           >  4:1058306/1‑135 (MQ=255)
                                                                                                      ttCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGGGTAAAAGTTTGa                                             >  4:377934/1‑149 (MQ=255)
                                                                                                      ttCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGa                                             >  1:1250224/1‑149 (MQ=255)
                                                                                                      ttCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGa                                             >  1:336099/1‑149 (MQ=255)
                                                                                                      ttCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATCAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACgg                                                           <  3:1058306/135‑1 (MQ=255)
                                                                                                       tCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGaa                                            <  4:1072789/149‑1 (MQ=255)
                                                                                                       tCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGaa                                            <  4:743339/149‑1 (MQ=255)
                                                                                                            ttCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGt                                       <  4:229873/149‑1 (MQ=255)
                                                                                                            ttCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGt                                       <  2:1334595/149‑1 (MQ=255)
                                                                                                              ccTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTCTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTgg                                     <  1:1181567/149‑1 (MQ=255)
                                                                                                                    ccATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGAc                               <  1:166375/149‑1 (MQ=255)
                                                                                                                      aTGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCt                             >  4:159239/1‑149 (MQ=255)
                                                                                                                      aTGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCt                             <  3:1314636/149‑1 (MQ=255)
                                                                                                                       tGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTg                            >  3:463541/1‑149 (MQ=255)
                                                                                                                       tGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTg                            >  2:1430870/1‑149 (MQ=255)
                                                                                                                        ggCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGa                           <  2:551535/149‑1 (MQ=255)
                                                                                                                          ccTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGaca                         >  1:932526/1‑149 (MQ=255)
                                                                                                                           cTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGacac                        >  4:256484/1‑149 (MQ=255)
                                                                                                                              tACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCGAGACCTGACACaga                     <  3:111821/149‑1 (MQ=255)
                                                                                                                              tACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACaga                     >  1:1469267/1‑149 (MQ=255)
                                                                                                                              tACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACaga                     <  2:595644/149‑1 (MQ=255)
                                                                                                                                 ttATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGc                                                         >  3:694940/1‑110 (MQ=255)
                                                                                                                                 ttATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACaga                     >  2:653150/1‑146 (MQ=255)
                                                                                                                                 ttATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACaga                     <  1:653150/146‑1 (MQ=255)
                                                                                                                                 ttATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGa                  <  4:1165600/149‑1 (MQ=255)
                                                                                                                                    tCGGCTATGTCACATAAGTGACGATGAACGGCGAACTTACTGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTTCCATGCAGCGTGCAAATTGACGGGCGTAAAAGTTTGAAGCATTGCCAAGACCTGACACAGAGGATTc               <  4:910977/149‑1 (MQ=255)
                                                                                                                                       gTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGa            <  4:1441043/149‑1 (MQ=255)
                                                                                                                                       gTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGa            <  4:769800/149‑1 (MQ=255)
                                                                                                                                       gTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGa            <  1:979350/149‑1 (MQ=255)
                                                                                                                                       gTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGa            >  4:574318/1‑149 (MQ=255)
                                                                                                                                       gTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGa            >  2:1159247/1‑149 (MQ=255)
                                                                                                                                        ttATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGAt           <  2:1287999/149‑1 (MQ=255)
                                                                                                                                          tcgTCACTTATGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACgg                                                                       <  4:1323187/85‑1 (MQ=255)
                                                                                                                                          tcgTCACTTATGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACgg                                                                       >  3:1323187/3‑87 (MQ=255)
                                                                                                                                          tcgTCACTTATGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGt                                                 >  1:1395591/3‑109 (MQ=255)
                                                                                                                                          tcgTCACTTATGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTg         >  1:738030/3‑149 (MQ=255)
                                                                                                                                           tGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGa        <  4:723361/149‑1 (MQ=255)
                                                                                                                                             tCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCt                             <  2:1217988/126‑1 (MQ=255)
                                                                                                                                             tCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCt                             >  1:1217988/1‑126 (MQ=255)
                                                                                                                                             tCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATg      <  4:620226/149‑1 (MQ=255)
                                                                                                                                              cacaTAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGt                                       >  2:1243992/1‑115 (MQ=255)
                                                                                                                                              cacaTAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGt                                       <  1:1243992/115‑1 (MQ=255)
                                                                                                                                               acaTAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATGTc    <  3:1081474/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GTAAATATTCAGCAACTGCACGGGGTCATGGTGCTCGCCGCCCAGCGTATTGAGATGGTCACGCAACGCCTCAACGGCACTGTGATTAACAATAAACTTTAATTCGATTTCCTGAGCCATGGCCTTTACTTATCGGTTATGTCACATAAGTGACGATGAACGGCGAACTTAATGCGATCTTTTTTGTCAGTAGATAGTATTTTGCGCCAAATTGCCATGCAACGGGCAATTTGACGGGCGTAAAAGTTTGAAGCAGTGGCAAGACCTGACACAGAGGATTCCGATTGATGTCGA  >  NC_000913/3200846‑3201139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: