Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,202,685 A→G 5.2% E265E (GAA→GAG cca → fused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,202,6850AG 5.2% 581.4 / 12.3 172E265E (GAA→GAGccafused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase
Reads supporting (aligned to +/- strand):  ref base A (86/77);  new base G (5/4);  total (91/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

Only 100 of 176 total aligned reads displayed.

TTCCGGCCCCTGCCAAGTGGCATCCGGAAATCGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACTGGTGGCTGAGTTTCACGATCTCATCCACACCTTCCCAATGCTGA  >  NC_000913/3202537‑3202833
                                                                                                                                                    |                                                                                                                                                    
ttCCGGCCCCTGCCAAGTGGCATCCGGAAATCGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGaa                                                                                                                                                      <  4:424229/149‑1 (MQ=255)
  ccGGCCCCTGCCAAGTGGCATCCGGAAATCGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAAct                                                                                                                                                    >  4:348877/1‑149 (MQ=255)
      ccccTGCCAAGTGGCATCCGGAAATCGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTgg                                                                                                                                                <  3:411139/149‑1 (MQ=255)
       cccTGCCAAGTGGCATCCGGAAATCGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAATGGCTGACGCCGCCAGAACTCTGGc                                                                                                                                               >  4:1115686/1‑149 (MQ=255)
         cTGCCAAGTGGCATCCGGAAATCGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCcg                                                                                                                                             >  3:120538/1‑149 (MQ=255)
          tGCCAAGTGGCATCCGGAAATCGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTggcggc                                                                                                                                            <  4:121311/149‑1 (MQ=255)
           gCCAAGTGGCATCCGGAAATCGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCcgcg                                                                                                                                           >  4:1325415/1‑149 (MQ=255)
            ccAAGTGGCATCCGGAAATCGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCcgc                                                                                                                                            >  1:1011564/1‑147 (MQ=255)
            ccAAGTGGCATCCGGAAATCGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGt                                                                                                                                          >  4:1003191/1‑149 (MQ=255)
              aaGTGGCATCCGGAAATCGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtca                                                                                                                                        >  4:829296/1‑149 (MQ=255)
                   gCATCCGGAAATCGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatg                                                                                                                                   >  3:1183005/1‑149 (MQ=255)
                   gCATCCGGAAATCGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatg                                                                                                                                   >  3:366682/1‑149 (MQ=255)
                        cGGAAATCGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggtcat                                                                                                                              <  4:495372/149‑1 (MQ=255)
                         ggAAATCGATAAGGGAATTCATACCTTAATGACGCTCTCTATGGCGACGATGCTGAGTACACAGGTAGATGTCCGTTTCTCAATTTTATGCCACGATCTAGGTAGAGGGCTGACTCCGCCTTAACTCTCATCACGTAAtcatggtcatg                                                                                                                             >  4:872876/1‑149 (MQ=255)
                                gATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCcggc                                                                                                                      >  2:377204/1‑149 (MQ=255)
                                 aTACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCcggcg                                                                                                                     >  1:16908/1‑149 (MQ=255)
                                  tACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCcggcgg                                                                                                                    <  1:1154504/149‑1 (MQ=255)
                                  tACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGACCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCcggcgg                                                                                                                    <  3:222213/149‑1 (MQ=255)
                                    cGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGgt                                                                                                                  <  1:1234938/149‑1 (MQ=255)
                                    cGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGgt                                                                                                                  <  4:961715/149‑1 (MQ=255)
                                    cGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGgt                                                                                                                  <  3:1346896/149‑1 (MQ=255)
                                    cGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGGCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGgt                                                                                                                  <  1:380546/149‑1 (MQ=255)
                                     gggTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGgtg                                                                                                                 >  1:1008770/1‑149 (MQ=255)
                                     gggTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGgtg                                                                                                                 >  1:1266508/1‑149 (MQ=255)
                                      ggTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGgtgt                                                                                                                <  1:264342/149‑1 (MQ=255)
                                      ggTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGgtgt                                                                                                                <  2:1482431/149‑1 (MQ=255)
                                       gTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGtt                                                                                                               >  4:706565/1‑149 (MQ=255)
                                           tCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGt                                                                                                           <  3:955215/149‑1 (MQ=255)
                                              tACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGt                                                                                                                                          >  3:964536/1‑115 (MQ=255)
                                              tACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTa                                                                                                         >  3:292690/1‑148 (MQ=255)
                                              tACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTa                                                                                                         <  4:292690/148‑1 (MQ=255)
                                               aCCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGt                                                                                                       <  3:517484/149‑1 (MQ=255)
                                               aCCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGt                                                                                                       <  2:1374965/149‑1 (MQ=255)
                                                ccTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTg                                                                                                      <  3:348877/149‑1 (MQ=255)
                                                 cTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagc                                                                                                                                      >  2:609163/1‑114 (MQ=39)
                                                 cTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagc                                                                                                                                      <  1:609163/116‑3 (MQ=39)
                                                 cTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTgg                                                                                                     >  4:1451744/1‑149 (MQ=255)
                                                 cTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTgg                                                                                                     >  1:194002/1‑149 (MQ=255)
                                                 cTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGATGACGCGGCCAGAGTTCTggcggc                                                                                                                                            <  2:1392538/110‑1 (MQ=255)
                                                 cTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGATGACGCGGCCAGAGTTCTGGcggc                                                                                                                                            >  1:1392538/1‑107 (MQ=255)
                                                  ttAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGa                                                                                                    >  3:1112138/1‑149 (MQ=255)
                                                  ttAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGa                                                                                                    >  3:775176/1‑149 (MQ=255)
                                                       gACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGccc                                                                                                                         >  2:930232/1‑123 (MQ=255)
                                                            tctcAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGc                                                                                          >  2:850531/1‑149 (MQ=255)
                                                            tctcAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGc                                                                                          >  4:737696/1‑149 (MQ=255)
                                                                aaTGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCcggcgg                                                                                                                    <  2:365058/119‑1 (MQ=255)
                                                                aaTGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCcggcgg                                                                                                                    >  1:365058/1‑119 (MQ=255)
                                                                aaTGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGc                                                                                      >  2:1454605/1‑149 (MQ=255)
                                                                aaTGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGc                                                                                      >  3:1405417/1‑149 (MQ=255)
                                                                    gcggcgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGaacaac                                                                                               <  2:1144569/136‑1 (MQ=255)
                                                                    gcggcgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCt                                                                                  >  3:1320737/1‑149 (MQ=255)
                                                                        cgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTgcgc                                                                              <  2:205879/149‑1 (MQ=255)
                                                                             cTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGaac                                                                                                  >  2:103845/1‑124 (MQ=255)
                                                                              tGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTa                                                                                             <  2:943634/128‑1 (MQ=255)
                                                                              tGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTa                                                                                             >  1:943634/1‑128 (MQ=255)
                                                                              tGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCa                                                                        >  1:478569/1‑149 (MQ=255)
                                                                              tGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCa                                                                        >  3:482186/1‑149 (MQ=255)
                                                                                 gTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAAt                                                                     >  4:935249/1‑149 (MQ=255)
                                                                                 gTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAAt                                                                     <  3:662685/149‑1 (MQ=255)
                                                                                 gTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAAt                                                                     >  2:534530/1‑149 (MQ=255)
                                                                                    cGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGaa                                                                  <  1:298281/149‑1 (MQ=255)
                                                                                     gCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGaaa                                                                 >  1:741507/1‑149 (MQ=255)
                                                                                            gATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGa                                                          >  4:765219/1‑149 (MQ=255)
                                                                                            gATGTCCGTTCCGCACCTTTACGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTACGTTAGTGGAAACACTATGACAGAGTCTGCGCGTGCAAAATGAAATTCGCGa                                                          <  4:775176/149‑1 (MQ=255)
                                                                                              tGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCa                                                                                        >  3:566792/1‑117 (MQ=255)
                                                                                                   gTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGcc                                                   >  4:810513/1‑149 (MQ=255)
                                                                                                       cGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGAc                                               <  4:274471/149‑1 (MQ=255)
                                                                                                        gCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACt                                              >  1:273521/1‑149 (MQ=255)
                                                                                                        gCAACTTTATGCCACGATCTCGGCAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACt                                              >  4:1269208/1‑149 (MQ=255)
                                                                                                         cAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACtg                                             >  2:678679/1‑149 (MQ=255)
                                                                                                          aaCTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACtgg                                            <  3:1167938/149‑1 (MQ=255)
                                                                                                          aaCTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACtgg                                            >  4:1167938/1‑149 (MQ=255)
                                                                                                          aaCTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACtgg                                            >  4:1294816/1‑149 (MQ=255)
                                                                                                           aCTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACtggt                                           <  3:523964/149‑1 (MQ=255)
                                                                                                            cTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTCCCAAATGAAATTCGCGATTTAGCCAGAAtggtg                                          <  1:422800/149‑1 (MQ=255)
                                                                                                             tttATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACtggtgg                                         <  3:737696/149‑1 (MQ=255)
                                                                                                                 tGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACTGGTGGCTGa                                     <  1:963778/149‑1 (MQ=255)
                                                                                                                 tGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACTGGTGGCTGa                                     <  1:1454605/149‑1 (MQ=255)
                                                                                                                   ccACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACTGGTGGCTGAGt                                   <  1:850531/149‑1 (MQ=255)
                                                                                                                    cACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACTGGTGGCTGAGtt                                  >  4:386239/1‑149 (MQ=255)
                                                                                                                    cACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACTGGTGGCTGAGtt                                  >  4:576827/1‑149 (MQ=255)
                                                                                                                    cACGATCTCGGTAAAGCGCTGACGCCGCCAGAACTCTGGCCGCGAAATCCTGGTCATGGCCCGCCGGGTGTTAGGATAGTGGAACAACTATGCCAGCGTCTGCGAGTGCCAAATGAAATTCGCGCTTTAGCCAGACTGGTGGCTGAgat                                  >  2:25937/1‑147 (MQ=255)
                                                                                                                       gATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACTGGTGGCTGAGTTTCa                               >  3:1021134/1‑149 (MQ=255)
                                                                                                                           tcGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACTGGTGGCTGAGTTTCACGAt                           >  4:1290347/1‑149 (MQ=255)
                                                                                                                           tcGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACTGGTGGCTGAGTTTCACGAt                           <  2:1266508/149‑1 (MQ=255)
                                                                                                                           tcGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACTGGTGGCTGAGTTTCACGAt                           <  1:344546/149‑1 (MQ=255)
                                                                                                                           tcGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACTGGTGGCTGAGTTTCACGAt                           <  3:797182/149‑1 (MQ=255)
                                                                                                                               tAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGAc                                               >  2:1201982/1‑125 (MQ=255)
                                                                                                                               tAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGAc                                               <  1:1201982/125‑1 (MQ=255)
                                                                                                                               caaaGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACTGGTGGCTGAGTTTCACGATCTCa                       <  1:1057721/148‑1 (MQ=255)
                                                                                                                                 aaGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACTGGTGGCTGAGTTTCACGATCTCATc                     <  4:482186/149‑1 (MQ=255)
                                                                                                                                 aaGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACTGGTGGCTGAGTTTCACGATCTCATc                     <  2:478569/149‑1 (MQ=255)
                                                                                                                                 aaGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACTGGTGGCTGAGTTTCACGATCTCATc                     <  1:760528/149‑1 (MQ=255)
                                                                                                                                  aGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCaaa                                                                      >  1:1437473/1‑99 (MQ=255)
                                                                                                                                   gggCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACTGGTGGCTGAGTTTCACGATCTCATCca                   >  3:591494/1‑149 (MQ=255)
                                                                                                                                   atgatGACGCGGCCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACTGGTGGCTGAGTTTCACGATCTCATCca                   >  4:972721/5‑149 (MQ=255)
                                                                                                                                     gCTGACGCCGCCAGAACTCTGGCCGCGTCACCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGTAATTCGCGATTTAGCCAGACTGGTGGCTGAGGTTCACGATCTCATCcaca                 >  2:783273/1‑149 (MQ=255)
                                                                                                                                              gccAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACTGGTGGCTGAGTTTCACGATCTCATCCACACCTTCCCaa        >  1:667479/1‑149 (MQ=255)
                                                                                                                                                   aaCTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGACATTCGCGATTTAGCCAGACTGGTGGCTGAGTTTCACGATCTCATCCacac                <  4:1197409/136‑1 (MQ=255)
                                                                                                                                                   aaCTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACTGGTGGCTGAGTTTCACGATCTCATCCacac                >  3:1197409/1‑136 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTCCGGCCCCTGCCAAGTGGCATCCGGAAATCGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACTGGTGGCTGAGTTTCACGATCTCATCCACACCTTCCCAATGCTGA  >  NC_000913/3202537‑3202833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: