Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,234,506 | G→A | 8.2% | intergenic (‑51/+233) | higB ← / ← rlmG | mRNA interferase toxin of the HigB‑HigA toxin‑antitoxin system/23S rRNA m(2)G1835 methyltransferase, SAM‑dependent |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,234,506 | 0 | G | A | 8.2% | 536.5 / 27.1 | 174 | intergenic (‑51/+233) | higB/rlmG | mRNA interferase toxin of the HigB‑HigA toxin‑antitoxin system/23S rRNA m(2)G1835 methyltransferase, SAM‑dependent |
Reads supporting (aligned to +/- strand): ref base G (70/88); new base A (7/7); total (78/96) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.82e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.75e-01 |
Only 100 of 175 total aligned reads displayed.
CCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGC > NC_000913/3234358‑3234651 | cccTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAg > 1:1354653/1‑149 (MQ=255) ccTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGa > 4:554628/1‑149 (MQ=255) ccTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGa > 4:657690/1‑149 (MQ=255) cTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCTGTTGCCAGAt < 3:1318619/149‑1 (MQ=255) cTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAt < 4:1067822/149‑1 (MQ=255) aaTCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCaa > 2:266619/1‑149 (MQ=255) aTCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAAc > 3:295176/1‑149 (MQ=255) tgtTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGAt > 2:565873/1‑149 (MQ=255) ccccAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAAt < 2:137657/149‑1 (MQ=255) ccccAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAAt > 1:1410905/1‑149 (MQ=255) ccAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAAttt > 4:1337330/1‑149 (MQ=255) ccAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAAttt < 4:295176/149‑1 (MQ=255) ccAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAAttt < 2:454313/149‑1 (MQ=255) agagCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTAt > 2:672854/1‑149 (MQ=255) gagCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATc > 3:1012714/1‑149 (MQ=255) ccaccaACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCATTATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCtgt < 4:707263/149‑1 (MQ=255) ccaccaACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑Tg < 4:1398471/128‑1 (MQ=255) ccaccaACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑Tg > 3:1398471/1‑128 (MQ=255) ccaccaACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCtgt < 1:809912/149‑1 (MQ=255) ccaACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCtgtgtg > 3:1392307/1‑149 (MQ=255) aaCTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGAc < 3:677533/149‑1 (MQ=255) aaCTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGAc > 3:18378/1‑149 (MQ=255) aaCTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGAc > 3:935653/1‑149 (MQ=255) aaCTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGAc < 4:1309881/149‑1 (MQ=255) tCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGc < 4:1199451/122‑1 (MQ=255) tCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGga > 2:814640/1‑149 (MQ=255) tCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAATC‑TGGCAACgg < 2:719511/127‑1 (MQ=255) ccGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGgaa < 1:656332/149‑1 (MQ=255) ccGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGgaa < 1:266619/149‑1 (MQ=255) cGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAGATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGgaag > 2:974350/1‑149 (MQ=255) gTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAATC‑TGGCAACGGAt < 1:845306/126‑1 (MQ=255) gTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAATC‑TGGCAACGGAt > 2:845306/1‑126 (MQ=255) ttttATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGgaagaa > 1:964824/1‑149 (MQ=255) ttttATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGgaagaa < 4:192725/149‑1 (MQ=255) tttATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAAt < 1:672854/149‑1 (MQ=255) aTGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATaaa < 3:554628/149‑1 (MQ=255) aTGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATaaa < 1:1454804/149‑1 (MQ=255) aTGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATaaa < 1:1444704/149‑1 (MQ=255) tGTTGCGGGTATTTTTCCGCAGTATCTTATAATGCTTTTTGAGTTATCAGGTGCATTCTTTATCACGTCCGTTTACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAAc < 3:1147722/149‑1 (MQ=255) gTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGaaaa > 2:637045/1‑149 (MQ=255) gTATTTATCCGCAGCATCTTTCAATGCTCTTTGAGTTATCAGGTGCATTCTTCATCTCGACCGTTGACAAATTGGCAATATGTTAACATCCCTGGCAAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGaaat > 2:200730/1‑148 (MQ=255) tATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGaaaaa < 1:177171/149‑1 (MQ=255) aTTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTTGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAAc < 2:284724/149‑1 (MQ=255) aTTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAAc < 2:483806/149‑1 (MQ=255) aTTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAAc < 2:297258/149‑1 (MQ=255) aTTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAAc < 1:40819/149‑1 (MQ=255) aTTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAAc < 4:1454282/149‑1 (MQ=255) tCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAGTATGATAACATCCGTTGCCAGAT‑TGGCAACGGGTGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAAc > 4:1390358/1‑135 (MQ=255) tCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGATGAATAAACGTAGAAAAACGCTAAACAAGAGaa < 4:1248722/149‑1 (MQ=255) tGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAaga > 4:1075244/1‑138 (MQ=255) tGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAaga < 3:1075244/138‑1 (MQ=255) tttttGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAAt > 1:1286349/1‑89 (MQ=255) tttttGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAAt < 2:1286349/89‑1 (MQ=255) tttGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGt > 2:647980/1‑149 (MQ=255) gAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGAc > 4:685217/1‑149 (MQ=255) ttATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAAt > 4:842590/1‑81 (MQ=255) ttATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAAt < 3:842590/81‑1 (MQ=255) gTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCTGTTGCCGGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGAttttt > 2:864452/1‑149 (MQ=255) gTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGAttttt > 3:1067658/1‑149 (MQ=255) tGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGAtttttt > 4:326129/1‑149 (MQ=255) cATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGt > 2:1174875/1‑149 (MQ=255) aTTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGtt > 4:918777/1‑149 (MQ=255) aTTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGtt > 1:1314408/1‑149 (MQ=255) aTTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGtt < 1:597030/149‑1 (MQ=255) aTTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGtt < 2:1151689/149‑1 (MQ=255) aTTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGtt > 2:1453357/1‑149 (MQ=255) ttcttcATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAggg < 4:798418/138‑1 (MQ=255) ttcttcATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAggg > 3:798418/1‑138 (MQ=255) ttcttcATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGttt > 1:634202/1‑149 (MQ=255) cttcATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTa < 3:190940/149‑1 (MQ=255) cttcATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTa < 3:122847/149‑1 (MQ=255) ttcATCACGTCCGTTGACAAATTGGCAATATGATAACATCTGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGAtttttt < 3:1337056/142‑1 (MQ=255) ttcATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATGTGTGTGAAGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTAt > 1:1070789/1‑149 (MQ=255) ttcATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTAt < 4:557733/149‑1 (MQ=255) ttcATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTAt > 4:1352713/1‑149 (MQ=255) tcaCGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGaa < 3:326129/149‑1 (MQ=255) tcaCGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGaa < 3:698238/149‑1 (MQ=255) aCGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAaga < 4:1028442/106‑1 (MQ=255) gTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGAtt > 2:975650/1‑149 (MQ=255) gACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCa > 3:1241449/1‑149 (MQ=255) cAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGa < 2:403783/149‑1 (MQ=255) aaaTTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGaa < 2:1314408/149‑1 (MQ=255) aaTTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAAc > 2:102331/1‑149 (MQ=255) ttGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTg < 2:799616/149‑1 (MQ=255) tGGCAATATGATAACATCCGTTGACAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTgg < 1:1174875/149‑1 (MQ=255) aaTATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAg > 4:483125/1‑149 (MQ=255) gATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACAtt > 4:1008057/1‑149 (MQ=255) gATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACAtt > 4:388117/1‑149 (MQ=255) tAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACTGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGc > 2:1219989/1‑149 (MQ=255) tAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGc < 3:1219174/149‑1 (MQ=255) aCATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCg > 3:1032415/1‑149 (MQ=255) aCAGCCGTTGCCAGAT‑TGGCAACGGATGAAATTATCGGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCg < 4:1282184/149‑1 (MQ=255) aTCCGTTGCCA‑ATCTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGt < 1:578953/107‑1 (MQ=255) tCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGAt < 3:918777/149‑1 (MQ=255) tCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGAt > 1:721515/1‑149 (MQ=255) tCCGTTGCCAATC‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTgg < 2:1083923/133‑1 (MQ=255) tCCGTTGCCAATC‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTgg > 1:1083923/1‑133 (MQ=255) tCCGTTGCCAATC‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGc < 2:582943/144‑1 (MQ=255) ccGTTGCCAATC‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTAt < 3:460926/110‑1 (MQ=255) tGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGAc < 1:565873/149‑1 (MQ=255) | CCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAT‑TGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGC > NC_000913/3234358‑3234651 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |