Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 533,846 T→C 7.4% intergenic (+20/‑70) allR → / → gcl glyoxylate‑inducible transcriptional repressor of all and gcl operons/glyoxylate carboligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913533,8460TC 7.4% 607.5 / 27.6 190intergenic (+20/‑70)allR/gclglyoxylate‑inducible transcriptional repressor of all and gcl operons/glyoxylate carboligase
Reads supporting (aligned to +/- strand):  ref base T (90/86);  new base C (7/7);  total (97/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.05e-02

Only 100 of 195 total aligned reads displayed.

GTTGCCGCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCGG  >  NC_000913/533698‑533994
                                                                                                                                                    |                                                                                                                                                    
gttgCCGCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGctct                                                                                                                                                      >  4:404508/1‑149 (MQ=255)
    ccGCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCgg                                                                                                                                                  >  2:680844/1‑149 (MQ=255)
     cGCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGa                                                                                                                                                 >  1:675393/1‑149 (MQ=255)
      gCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAg                                                                                                                                                >  4:27694/1‑149 (MQ=255)
           ctccatctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCCGCAGAGCggga                                                                                                                                           <  3:101738/149‑2 (MQ=255)
            tccatctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGtt                                                                                                                                          >  3:744778/1‑149 (MQ=255)
            tccatctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGtt                                                                                                                                          >  4:881774/1‑149 (MQ=255)
            tccatctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCAACCCGCTCCGCAGAGCgggat                                                                                                                                          <  2:1239618/149‑3 (MQ=255)
                 ctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGttttttt                                                                                                                                     <  4:1453855/149‑1 (MQ=255)
                 ctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGttttttt                                                                                                                                     <  4:1166989/149‑1 (MQ=255)
                 ctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGttttttt                                                                                                                                     <  1:997742/149‑1 (MQ=255)
                 ctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGttttttt                                                                                                                                     <  3:60230/149‑1 (MQ=255)
                   ccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGa                                                                                                                                   <  1:175624/149‑1 (MQ=255)
                       gCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACaaa                                                                                                                               >  2:1325161/1‑149 (MQ=255)
                         cTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAAt                                                                                                                             >  3:317579/1‑149 (MQ=255)
                          ttCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAAtt                                                                                                                            >  1:568475/1‑149 (MQ=255)
                          ttCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAAtt                                                                                                                            >  3:462863/1‑149 (MQ=255)
                             atcaAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGa                                                                                                                         >  4:1100516/1‑149 (MQ=255)
                              tcaAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCATATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGaa                                                                                                                        >  3:1380999/1‑149 (MQ=255)
                              tcaAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGaa                                                                                                                        >  2:1442971/1‑149 (MQ=255)
                               caAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGaaa                                                                                                                       <  1:1325161/149‑1 (MQ=255)
                                 aGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGt                                                                                                                     >  1:574099/1‑149 (MQ=255)
                                  gACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGtt                                                                                                                    <  2:626502/149‑1 (MQ=255)
                                   aCTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTg                                                                                                                   <  2:55419/149‑1 (MQ=255)
                                     tGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGa                                                                                                                 >  1:910315/1‑149 (MQ=255)
                                        cAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGaaaa                                                                                                              >  4:1039100/1‑149 (MQ=255)
                                          gaagaTCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGAGTGAAAGTACATCCATAATGTCTGTAGCATCCCGCTCTTCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAAt                                                                                                            >  1:1059368/1‑149 (MQ=255)
                                          gaagaTCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAAt                                                                                                            <  3:1100516/149‑1 (MQ=255)
                                           aagaTCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAAtt                                                                                                           <  3:710486/149‑1 (MQ=255)
                                            agaTCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAAttt                                                                                                          >  2:926918/1‑149 (MQ=255)
                                             gaTCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAATtttt                                                                                                         >  1:829707/1‑149 (MQ=255)
                                              aTCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTgg                                                                                                                  <  1:731643/139‑1 (MQ=255)
                                              aTCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTc                                                                                                        >  2:1175037/1‑149 (MQ=255)
                                              aTCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTc                                                                                                        <  4:1020515/149‑1 (MQ=255)
                                              aTCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTc                                                                                                        >  4:458519/1‑149 (MQ=255)
                                              aTCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTc                                                                                                        <  4:813667/149‑1 (MQ=255)
                                                 gTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCaa                                                                                                     <  4:744778/149‑1 (MQ=255)
                                                 gTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCaa                                                                                                     >  2:1357903/1‑149 (MQ=255)
                                                         agtcagGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATaga                                                                                             <  1:28165/149‑1 (MQ=255)
                                                         agtcagGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATaga                                                                                             <  3:85236/149‑1 (MQ=255)
                                                          gtcagGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATagag                                                                                            >  2:824588/1‑149 (MQ=255)
                                                           tcagGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTAGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCACTAAATAGAgg                                                                                           >  4:631593/1‑149 (MQ=255)
                                                            cagGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGt                                                                                          <  1:532752/149‑1 (MQ=255)
                                                             agGGTGAGCTGGTCGGAGACACCGCCCGCGATATCAGCACGGCGTGGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAAGAGAGGTa                                                                                         >  4:1364315/1‑149 (MQ=255)
                                                                     cTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATaaa                                                                                 <  4:281194/149‑1 (MQ=255)
                                                                        gTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGa                                                                                                                                   <  2:392556/96‑1 (MQ=255)
                                                                        gTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGa                                                                                                                                   >  1:392556/1‑96 (MQ=255)
                                                                         tCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATaa                                                                                  <  4:162445/144‑1 (MQ=255)
                                                                         tCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATgg                                                                             >  2:251629/1‑149 (MQ=255)
                                                                           agagACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCa                                                                           >  4:1137082/1‑149 (MQ=255)
                                                                             agaCACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCaaa                                                                         >  1:1235756/1‑149 (MQ=255)
                                                                              gaCACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCaaaa                                                                        >  4:219012/1‑149 (MQ=255)
                                                                                 acCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATg                                                                     >  2:284661/1‑149 (MQ=255)
                                                                                 acCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATg                                                                     >  2:485060/1‑149 (MQ=255)
                                                                                   cgcccgcGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATgag                                                                   <  3:812268/149‑1 (MQ=255)
                                                                                    gcccgcGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATgaga                                                                  <  3:1043352/149‑1 (MQ=255)
                                                                                    gcccgcGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATgaga                                                                  <  1:255730/149‑1 (MQ=255)
                                                                                        gcgATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCg                                                              >  3:838799/1‑149 (MQ=255)
                                                                                          gATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGtt                                                            <  1:1336339/149‑1 (MQ=255)
                                                                                              tCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGAcg                                                        >  2:1073028/1‑149 (MQ=255)
                                                                                                       cGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAatgt                                               <  3:1437991/149‑1 (MQ=255)
                                                                                                        gTTGGGACTGATAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTAGACGCGGCAatgta                                              <  3:948247/149‑1 (MQ=255)
                                                                                                        gTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAAt                                                                      <  2:427559/125‑1 (MQ=255)
                                                                                                           gggACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAACAATGAGAGCCGTTGACGCGGCAatgtatgt                                           >  3:1450075/1‑149 (MQ=255)
                                                                                                            ggACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTAtgtg                                          <  2:813490/149‑1 (MQ=255)
                                                                                                             gACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATaaaa                                                                                >  2:1127702/1‑110 (MQ=255)
                                                                                                             gACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGc                                                    <  4:1413519/138‑1 (MQ=255)
                                                                                                              aCTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCt                                        >  2:1085057/1‑149 (MQ=255)
                                                                                                                tGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTgg                                      >  4:59684/1‑149 (MQ=255)
                                                                                                                tGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTgg                                      >  2:1312144/1‑149 (MQ=255)
                                                                                                                  aaaGNACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGgag                                    >  1:1414633/1‑149 (MQ=255)
                                                                                                                  aaaGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGgag                                    >  3:229584/1‑149 (MQ=255)
                                                                                                                        caTCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGagaaagaa                              <  1:602504/149‑1 (MQ=255)
                                                                                                                        caTCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGagaaagaa                              <  3:417443/149‑1 (MQ=255)
                                                                                                                         aTCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAaagaag                             >  3:19932/1‑149 (MQ=255)
                                                                                                                         aTCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAaagaag                             <  1:1124387/149‑1 (MQ=255)
                                                                                                                         aTCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAaagaag                             <  3:473477/149‑1 (MQ=255)
                                                                                                                          tCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAAtttt                                                                                                         >  4:260060/1‑72 (MQ=255)
                                                                                                                            cATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATagag                                                                                            <  3:1275607/83‑1 (MQ=255)
                                                                                                                            cATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATagag                                                                                            >  4:1275607/1‑83 (MQ=255)
                                                                                                                            cATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTa                          >  3:974703/1‑149 (MQ=255)
                                                                                                                              tAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATc                        >  1:809749/1‑149 (MQ=255)
                                                                                                                               aaTGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGaa                                                                                     <  1:814195/87‑1 (MQ=255)
                                                                                                                                aTGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCAc                      >  1:948864/1‑149 (MQ=255)
                                                                                                                                     tgtcGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCg                 >  2:793974/1‑149 (MQ=255)
                                                                                                                                      aaaaaaacccGCTCCGCAGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGc                >  3:258723/8‑149 (MQ=255)
                                                                                                                                        aaaaacccGCTCCGCAGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGagaaagaa                              <  3:886446/128‑1 (MQ=255)
                                                                                                                                         gCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCtt             >  4:494271/1‑149 (MQ=255)
                                                                                                                                          cATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTc            >  2:1384348/1‑149 (MQ=255)
                                                                                                                                          cATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTc            <  2:572620/149‑1 (MQ=255)
                                                                                                                                           aTCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCg           <  2:856907/149‑1 (MQ=255)
                                                                                                                                           aTCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCg           <  4:1312832/149‑1 (MQ=255)
                                                                                                                                            tCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCgg          >  4:3601/1‑149 (MQ=255)
                                                                                                                                            acccGCTCCGCAGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATagag                                                                                            <  1:235380/66‑1 (MQ=39)
                                                                                                                                            acccGCTCCGCAGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATagag                                                                                            >  2:235380/2‑67 (MQ=39)
                                                                                                                                             cccGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGgt         >  1:539119/1‑149 (MQ=255)
                                                                                                                                              ccGATCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGgtg        <  4:984549/149‑1 (MQ=255)
                                                                                                                                                  tctGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTcc    >  2:1965/1‑149 (MQ=255)
                                                                                                                                                    tGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCgg  >  1:126611/1‑149 (MQ=255)
                                                                                                                                                    tGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCgg  <  1:1067612/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTTGCCGCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCGG  >  NC_000913/533698‑533994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: