Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,740,791 G→C 5.4% G363R (GGC→CGC)  avtA → valine‑pyruvate aminotransferase; transaminase C; alanine‑valine transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,740,7910GC 5.4% 2038.5 / 75.9 574G363R (GGC→CGC) avtAvaline‑pyruvate aminotransferase; transaminase C; alanine‑valine transaminase
Reads supporting (aligned to +/- strand):  ref base G (258/284);  new base C (14/17);  total (273/301)
Fisher's exact test for biased strand distribution p-value = 8.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01

Only 100 of 602 total aligned reads displayed.

TGTTCAGGAAACTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGC  >  NC_000913/3740643‑3740939
                                                                                                                                                    |                                                                                                                                                    
     aGGAAACTATCGCCATCATTCGCCGCGATTTACCGGACAATCGCTGCCTGATTCATAAACCGGAGGGAGCCATTTTCCTGTGGCTGTGTTTTAAGGATTTGCCCATTAAGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTg                                                                                                                                                 >  4:365678/1‑149 (MQ=255)
       gAAACTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCt                                                                                                                                               >  2:1246800/1‑149 (MQ=255)
         aaCTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGa                                                                                                                                             <  3:53566/149‑1 (MQ=255)
         aaCTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGa                                                                                                                                             <  3:816043/149‑1 (MQ=255)
         aaCTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGa                                                                                                                                             <  3:120699/149‑1 (MQ=255)
             aTCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGt                                                                                                                                         >  1:1003946/1‑149 (MQ=255)
              tCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTg                                                                                                                                        >  1:960471/1‑149 (MQ=255)
              tCGCCATCATACGCCGCTATTTACCGGAAACTCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACCACCCAGCAGCTCTATCAGCGCCTGAAAGCATGCCGCGTGCTgttagga                                                                                                                                        <  4:520564/149‑7 (MQ=255)
                gCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGcc                                                                                                                                      >  1:1129142/1‑149 (MQ=255)
                gCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGcc                                                                                                                                      <  1:1311717/149‑1 (MQ=255)
                gCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGcc                                                                                                                                      >  1:69334/1‑149 (MQ=255)
                  catcatTCGCCTCTATTTAGCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGACTATGGTTTAAGGATTTGTCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCgg                                                                                                                                    >  4:1008188/1‑149 (MQ=255)
                  catcatTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCgg                                                                                                                                    <  2:1106740/149‑1 (MQ=255)
                  catcatTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCgg                                                                                                                                    <  3:231684/149‑1 (MQ=255)
                   atcatTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCggg                                                                                                                                   >  2:387322/1‑149 (MQ=255)
                   atcatTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCggg                                                                                                                                   >  4:200140/1‑149 (MQ=255)
                        tCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGcaca                                                                                                                              >  4:867522/1‑149 (MQ=255)
                            cgcTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAActt                                                                                                                          >  2:290365/1‑149 (MQ=255)
                                aTTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACttcttc                                                                                                                      <  2:14942/149‑1 (MQ=255)
                                       ggAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGc                                                                                                               <  3:1363876/149‑1 (MQ=255)
                                        gAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATg                                                                                                                                           <  3:633596/120‑1 (MQ=255)
                                        gAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCt                                                                                                              >  2:974535/1‑149 (MQ=255)
                                        gAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCt                                                                                                              >  4:1050525/1‑149 (MQ=255)
                                          aaaTCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTgg                                                                                                            <  2:536386/149‑1 (MQ=255)
                                           aaTCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGa                                                                                                           <  1:123892/149‑1 (MQ=255)
                                               gCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATaaa                                                                                                       >  2:417834/1‑149 (MQ=255)
                                                cTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAAc                                                                                                      <  2:201726/149‑1 (MQ=255)
                                                      gATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACttcttc                                                                                                                      <  1:391048/127‑1 (MQ=255)
                                                       aTTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCAcgcg                                                                                                                                                      <  4:1442030/94‑1 (MQ=255)
                                                        ttCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCg                                                                                              >  3:113050/1‑149 (MQ=255)
                                                        ttCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCg                                                                                              >  4:1108646/1‑149 (MQ=255)
                                                        ttCATAAACCGCAAGGAGCCATTTTCCTCTGGATATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGGGCCTGAAAGGACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCg                                                                                              >  3:1264271/1‑149 (MQ=255)
                                                           aTAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCAt                                                                                           >  4:293346/1‑149 (MQ=255)
                                                            tAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTg                                                                                                             <  1:580832/130‑1 (MQ=255)
                                                            tAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATa                                                                                          >  2:1032042/1‑149 (MQ=255)
                                                              aaCCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCGGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACg                                                                                        >  4:1245474/1‑149 (MQ=255)
                                                              aaCCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACg                                                                                        >  2:544463/1‑149 (MQ=255)
                                                              aaCCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACg                                                                                        >  1:324064/1‑149 (MQ=255)
                                                                ccGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCa                                                                                      >  4:950532/1‑149 (MQ=255)
                                                                ccGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCa                                                                                      <  4:118260/149‑1 (MQ=255)
                                                                 cGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCggg                                                                                                                                   <  3:60248/103‑1 (MQ=255)
                                                                    aaGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCaa                                                                                  <  2:465797/149‑1 (MQ=255)
                                                                       gAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGt                                                                               <  3:764003/149‑1 (MQ=255)
                                                                        aGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCa                                                                                      >  1:72735/1‑141 (MQ=255)
                                                                        aGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTa                                                                              <  4:786172/149‑1 (MQ=255)
                                                                         cccATTTTCCTCTGGCTATGGTCTAAGGATTTGCCCATTACGACCAAGGAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGCCACAACTTCTTCCCAGGACTGGATAAACCGTGGTCGCATACTCATCAATGTAt                                                                             <  2:1066430/148‑1 (MQ=255)
                                                                           cATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATgc                                                                           <  4:558128/149‑1 (MQ=255)
                                                                                tCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATg                                                                      <  1:98770/149‑1 (MQ=255)
                                                                                  ctctGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGaa                                                                    <  3:1236876/149‑1 (MQ=255)
                                                                                  ctctGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGaa                                                                    >  2:342643/1‑149 (MQ=255)
                                                                                  ctctGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGaa                                                                    <  2:723502/149‑1 (MQ=255)
                                                                                  ctctGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGaa                                                                    <  2:258160/149‑1 (MQ=255)
                                                                                   tctGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTg                                                                                                  <  4:1130491/118‑1 (MQ=255)
                                                                                   tctGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAAc                                                                   >  2:625675/1‑149 (MQ=255)
                                                                                   tctGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAAc                                                                   <  2:1157072/149‑1 (MQ=255)
                                                                                   tctGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGACACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAAc                                                                   >  3:896810/1‑149 (MQ=255)
                                                                                     tGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTa                                                                 <  1:1170578/149‑1 (MQ=255)
                                                                                       gCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTAGCCGCATACGCATCAATGTATGCGCATGAACTACg                                                               <  4:404406/149‑1 (MQ=255)
                                                                                         tATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGt                                                                                                                                         >  4:1203178/1‑73 (MQ=255)
                                                                                         tATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTa                                                             <  3:173222/149‑1 (MQ=255)
                                                                                         tATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTa                                                             <  1:289375/149‑1 (MQ=255)
                                                                                              tttAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCaga                                                        >  4:268091/1‑149 (MQ=255)
                                                                                                tAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGc                                                      >  2:858936/1‑149 (MQ=255)
                                                                                                 aaGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGcc                                                     <  3:656094/149‑1 (MQ=255)
                                                                                                  aGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCg                                                    >  1:1042299/1‑149 (MQ=255)
                                                                                                          cccATTACGACAAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAAt                                            >  3:944010/1‑149 (MQ=255)
                                                                                                           ccATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATgcgc                                                                         <  1:228712/119‑1 (MQ=255)
                                                                                                           ccATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTa                                                             >  4:1324382/1‑131 (MQ=255)
                                                                                                           ccATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAAtt                                           >  4:319687/1‑149 (MQ=255)
                                                                                                           ccATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGAGCCGGGGCACAACTCCTGCCCAGGGCTGGATAAACCGTGGCTGGATACGCATCAATGTATGGCGATTAACTACGTACCAGAGCCTGAGAAAAtt                                           <  2:808159/149‑1 (MQ=255)
                                                                                                             aTTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGa                                         <  4:698829/149‑1 (MQ=255)
                                                                                                               tACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAgg                                       >  1:4375/1‑149 (MQ=255)
                                                                                                                   aCCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCggg                                   <  4:604407/149‑1 (MQ=255)
                                                                                                                   aCCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCggg                                   <  2:900980/149‑1 (MQ=255)
                                                                                                                       agcagcTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTg                               >  3:295408/1‑149 (MQ=255)
                                                                                                                       agcagcTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTg                               <  3:1431994/149‑1 (MQ=255)
                                                                                                                         cagcTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGaa                             <  3:365678/149‑1 (MQ=255)
                                                                                                                             tctATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGAtt                         >  1:541419/1‑149 (MQ=255)
                                                                                                                             tctATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGAtt                         >  3:714852/1‑149 (MQ=255)
                                                                                                                              ctATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTc                        <  2:690041/149‑1 (MQ=255)
                                                                                                                                aTCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAAtt                                           >  3:50057/1‑128 (MQ=255)
                                                                                                                                aTCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTg                      <  3:1058005/149‑1 (MQ=255)
                                                                                                                                aTCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTg                      <  3:1318597/149‑1 (MQ=255)
                                                                                                                                  cAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGc                    <  3:406896/149‑1 (MQ=255)
                                                                                                                                  cAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGc                    <  2:59129/149‑1 (MQ=255)
                                                                                                                                   aGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCg                   >  4:440105/1‑149 (MQ=255)
                                                                                                                                      gcCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGaa                <  1:21006/149‑1 (MQ=255)
                                                                                                                                      gcCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGaa                <  2:1369250/149‑1 (MQ=255)
                                                                                                                                        cTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAaga              <  3:1108646/149‑1 (MQ=255)
                                                                                                                                        cTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAaga              <  1:489751/149‑1 (MQ=255)
                                                                                                                                         tGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAAc                                                                   <  4:1403373/95‑1 (MQ=255)
                                                                                                                                          gAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAgaga            <  1:1472405/149‑1 (MQ=255)
                                                                                                                                           tcaGCACGCCGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGAt           >  1:1363836/3‑149 (MQ=255)
                                                                                                                                            aaGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAagag             <  4:646045/146‑1 (MQ=255)
                                                                                                                                               cACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGaa       >  2:1433432/1‑149 (MQ=255)
                                                                                                                                               cACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGaa       >  2:577395/1‑149 (MQ=255)
                                                                                                                                                aCGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGaaa      <  1:870764/149‑1 (MQ=255)
                                                                                                                                                aCGCCGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGaaa      <  4:163009/149‑1 (MQ=255)
                                                                                                                                                  gcggcgTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAGCTACGTATCAGAGCCGGAGAAGATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAaga    <  2:221233/149‑1 (MQ=255)
                                                                                                                                                    ggcgTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGc  <  1:792007/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGTTCAGGAAACTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGC  >  NC_000913/3740643‑3740939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: