Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 543,210 A→T 5.3% intergenic (+177/+51) glxK → / ← allE glycerate kinase II/S‑ureidoglycine aminohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913543,2100AT 5.3% 512.7 / 13.3 147intergenic (+177/+51)glxK/allEglycerate kinase II/S‑ureidoglycine aminohydrolase
Reads supporting (aligned to +/- strand):  ref base A (70/69);  new base T (4/4);  total (74/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.07e-01

Only 100 of 152 total aligned reads displayed.

ATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGCCCA  >  NC_000913/543063‑543358
                                                                                                                                                   |                                                                                                                                                    
atCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATaaa                                                                                                                                                     >  3:457473/1‑149 (MQ=255)
atCGCATAAGAGTGCACTACTCATGCCGGATGCGGCATGTGTACCATATCCTTCCTGAAATTCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATaaa                                                                                                                                                     <  3:275975/149‑1 (MQ=255)
    cATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGt                                                                                                                                                 >  4:384753/1‑149 (MQ=255)
    cATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGt                                                                                                                                                 >  4:603773/1‑149 (MQ=255)
    cATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGt                                                                                                                                                 >  2:309628/1‑149 (MQ=255)
     aTAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTg                                                                                                                                                <  1:1027213/149‑1 (MQ=255)
     aTAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTg                                                                                                                                                <  2:230138/149‑1 (MQ=255)
     aTAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTg                                                                                                                                                <  1:1114322/149‑1 (MQ=255)
      tAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTgg                                                                                                                                               <  1:1221062/149‑1 (MQ=255)
       aaGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGc                                                                                                                                              <  4:457473/149‑1 (MQ=255)
             gCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATc                                                                                                                                        <  2:1246222/149‑1 (MQ=255)
              cAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATcc                                                                                                                                       <  4:767591/149‑1 (MQ=255)
              cAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATcc                                                                                                                                       <  2:528749/149‑1 (MQ=255)
              cAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATcc                                                                                                                                       <  1:1441933/149‑1 (MQ=255)
                gTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCaa                                                                                                                                     <  3:1061949/149‑1 (MQ=255)
                  aCTCATGCCGCATCCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACa                                                                                                                                   >  3:73078/1‑149 (MQ=255)
                    tCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATa                                                                                                                                 >  2:748906/1‑149 (MQ=255)
                    tCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATa                                                                                                                                 >  1:1368749/1‑149 (MQ=255)
                    tCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATa                                                                                                                                 >  3:1389409/1‑149 (MQ=255)
                    tCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATa                                                                                                                                 <  3:18342/149‑1 (MQ=255)
                    tCATGCCGCATCCGGCATGAGTACTATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATa                                                                                                                                 >  3:823460/1‑149 (MQ=255)
                    tCATGCCGCATCCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATa                                                                                                                                 >  4:638583/1‑149 (MQ=255)
                       tGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCt                                                                                                                              >  2:1236723/1‑149 (MQ=255)
                       tGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCt                                                                                                                              >  2:1376152/1‑149 (MQ=255)
                        gCCGGATTCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTa                                                                                                                             >  2:795797/1‑149 (MQ=255)
                           ggATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTaaaa                                                                                                                          <  1:297215/149‑1 (MQ=255)
                             aTGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAaat                                                                                                                        <  4:80348/149‑1 (MQ=255)
                               gCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAaataa                                                                                                                      <  3:1383791/149‑1 (MQ=255)
                                  gCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTaaaaa                                                                                                                         <  2:963283/143‑1 (MQ=255)
                                  gCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCa                                                                                                                   >  3:1174401/1‑149 (MQ=255)
                                  gCATGAGGACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCa                                                                                                                   >  2:800497/1‑149 (MQ=255)
                                         tACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGt                                                                                                            <  3:384753/149‑1 (MQ=255)
                                         tACCAAATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAATATCCAACATAGCTAAAAATAATCAGGCGAGt                                                                                                            >  1:423573/1‑149 (MQ=255)
                                          aCCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTg                                                                                                           <  4:173435/149‑1 (MQ=255)
                                           ccATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATATAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTgg                                                                                                          >  1:75466/1‑149 (MQ=255)
                                              tatCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTg                                                                                                           >  1:997089/1‑145 (MQ=255)
                                                   ttcctGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACtt                                                                                                  >  1:672222/1‑149 (MQ=255)
                                                        gAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAAtc                                                                                             >  4:1250617/1‑149 (MQ=255)
                                                            accgcgCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTa                                                                                         <  4:1400069/147‑1 (MQ=255)
                                                                   aaaTTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTcgcg                                                                                  <  4:515880/149‑1 (MQ=255)
                                                                    aaTTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCgg                                                                                 >  1:125232/1‑149 (MQ=255)
                                                                      ttCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGtt                                                                               >  1:1289370/1‑149 (MQ=255)
                                                                       tCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTa                                                                              <  3:419928/149‑1 (MQ=255)
                                                                       tCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTa                                                                              <  2:1289370/149‑1 (MQ=255)
                                                                          atatatTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACaa                                                                           >  4:1408046/1‑149 (MQ=255)
                                                                              atTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCtt                                                                       <  4:1130271/149‑1 (MQ=255)
                                                                              atTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCtt                                                                       >  3:232214/1‑149 (MQ=255)
                                                                                tGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTc                                                                     <  3:603773/149‑1 (MQ=255)
                                                                                tGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTc                                                                     <  4:823460/149‑1 (MQ=255)
                                                                                tGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTc                                                                     <  3:1303807/149‑1 (MQ=255)
                                                                                 gCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCg                                                                    <  4:1110819/149‑1 (MQ=255)
                                                                                  cAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGa                                                                   <  1:1236723/149‑1 (MQ=255)
                                                                                  cAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGa                                                                   <  1:1376152/149‑1 (MQ=255)
                                                                                  cAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGa                                                                   <  4:518091/149‑1 (MQ=255)
                                                                                   agagATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAg                                                                  >  4:1006889/1‑149 (MQ=255)
                                                                                   agagATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAg                                                                  >  4:397754/1‑149 (MQ=255)
                                                                                   agagATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAg                                                                  >  4:158848/1‑149 (MQ=255)
                                                                                   agagATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAg                                                                  >  4:310161/1‑149 (MQ=255)
                                                                                       aTCATATAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTaaa                                                              <  4:422054/149‑1 (MQ=255)
                                                                                        tCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAat                                                             >  3:47541/1‑149 (MQ=255)
                                                                                           tGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAAtata                                                          <  4:756115/149‑1 (MQ=255)
                                                                                            gTAGGCCTAATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAg                                                         <  4:1313885/149‑1 (MQ=255)
                                                                                              aGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATc                                                                                                                    <  3:672621/88‑1 (MQ=255)
                                                                                              aGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATc                                                                                                                    >  4:672621/1‑88 (MQ=255)
                                                                                                   tGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTa                                                                                         >  3:998993/1‑110 (MQ=255)
                                                                                                   tGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACg                                                  >  1:162897/1‑149 (MQ=255)
                                                                                                      tAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCtt                                               <  1:748906/149‑1 (MQ=255)
                                                                                                      tAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCtt                                               <  3:562889/149‑1 (MQ=255)
                                                                                                       aagcgaagcgCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTc                                              >  3:696986/1‑149 (MQ=255)
                                                                                                           gaagcgCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTcacca                                          >  1:1279221/1‑149 (MQ=255)
                                                                                                                   tCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTac                                  >  1:716320/1‑149 (MQ=255)
                                                                                                                   tCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTac                                  >  3:815389/1‑149 (MQ=255)
                                                                                                                     aGGAAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTacac                                <  1:309628/149‑1 (MQ=255)
                                                                                                                      ggCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAATGCTTCACCACGCCCTACAcc                               >  2:568286/1‑149 (MQ=255)
                                                                                                                       gCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGa                                                                   >  4:83021/1‑112 (MQ=255)
                                                                                                                       gCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCa                              <  3:249567/149‑1 (MQ=255)
                                                                                                                         aaTGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATa                            >  4:1041476/1‑149 (MQ=255)
                                                                                                                           tGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAAc                          <  1:115119/149‑1 (MQ=255)
                                                                                                                           tGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAAc                          <  3:61247/149‑1 (MQ=255)
                                                                                                                                 aaaaaaaGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCt                    >  4:980278/1‑149 (MQ=255)
                                                                                                                                 aaaaaaaGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCt                    >  4:1167992/1‑149 (MQ=255)
                                                                                                                                   aaaaaGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGt                  >  4:265232/1‑149 (MQ=255)
                                                                                                                                       aGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAg              >  3:555982/1‑149 (MQ=255)
                                                                                                                                         ccACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGaa            <  3:1292085/149‑1 (MQ=255)
                                                                                                                                          cACGGTTTATACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCt                    >  1:477392/1‑140 (MQ=255)
                                                                                                                                          cACGGTTTATACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCt                    <  2:477392/140‑1 (MQ=255)
                                                                                                                                          cACGGTTTATACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTaaa               >  4:704677/1‑145 (MQ=255)
                                                                                                                                          cACGGTTTATACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAAt           >  2:1199108/1‑149 (MQ=255)
                                                                                                                                          cACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAAt           >  3:768507/1‑149 (MQ=255)
                                                                                                                                          cACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAAt           >  2:1027024/1‑149 (MQ=255)
                                                                                                                                           aCGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGccc                                     <  1:1213340/122‑1 (MQ=255)
                                                                                                                                           aCGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGccc                                     >  2:1213340/1‑122 (MQ=255)
                                                                                                                                           aCGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATa          >  4:1371352/1‑149 (MQ=255)
                                                                                                                                             ggTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAg        >  3:325514/1‑149 (MQ=255)
                                                                                                                                              gTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAgc       <  2:967839/149‑1 (MQ=255)
                                                                                                                                              gTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAgc       <  4:813876/149‑1 (MQ=255)
                                                                                                                                                ataAACCGTGGCAAAATCCCACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAgcgc     <  4:59187/149‑1 (MQ=255)
                                                                                                                                                  aaaCCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACaa                                                                           <  2:1075308/77‑1 (MQ=255)
                                                                                                                                                  aaaCCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACaa                                                                           >  1:1075308/1‑77 (MQ=255)
                                                                                                                                                   aaCCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGCCCa  >  4:711589/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGCCCA  >  NC_000913/543063‑543358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: