Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,807,638 T→G 6.5% E154A (GAA→GCA)  waaQ ← lipopolysaccharide core biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,807,6380TG 6.5% 2326.8 / 123.5 684E154A (GAA→GCA) waaQlipopolysaccharide core biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base T (307/330);  new base G (23/22);  total (330/354)
Fisher's exact test for biased strand distribution p-value = 7.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.49e-01

Only 100 of 699 total aligned reads displayed.

GCCTCGCTGTTGCAGCGCATCGATAACCTTAGAAAATTTATCGTTATCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTAAGTGTGTGAAGCTTTTTTTCCAAATACCATGCTGCCGATGACCAT  >  NC_000913/3807490‑3807785
                                                                                                                                                    |                                                                                                                                                   
 ccTCGCTGTTGCAGCGCATCGATAACCTTAGAAAATTTATCGTTATCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGACCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTc                                                                                                                                                    <  3:1180585/149‑1 (MQ=255)
  cTCGCTGTTGCAGCGCATCGATAACCTTAGAAAATTTATCGTTATCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCg                                                                                                                                                   <  3:640702/149‑1 (MQ=255)
  cTCGCTGTTGCAGCGCATCGATAACCTTAGAAAATTTATCGTTATCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCg                                                                                                                                                   <  2:1433706/149‑1 (MQ=255)
  cTCGCTGTTGCAGCGCATCGATAACCTTAGAAAATTTATCGTTATCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCg                                                                                                                                                   >  2:255127/1‑149 (MQ=255)
   tCGCTGTTGCAGCGCATCGATAACCTTAGAAAATTTATCGTTATCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCgg                                                                                                                                                  >  2:1287554/1‑149 (MQ=255)
   tCGCTGTTGCAGCGCATCGATAACCTTAGAAAATTTATCGTTATCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCgg                                                                                                                                                  >  3:1150519/1‑149 (MQ=255)
   tCGCTGTTGCAGCGCATCGATAACCTTAGAAAATTTATCGTTATCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCgg                                                                                                                                                  <  1:227442/149‑1 (MQ=255)
        gTTGCAGCGCATCGATAACCTTAGAAAATTTATCGTTATCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTaa                                                                                                                                             >  4:162190/1‑149 (MQ=255)
            cAGCGCATCGATAACCTTAGAAAATTTATCGTTATCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTGCGGCGTAACTCa                                                                                                                                         <  2:686614/149‑1 (MQ=255)
              gcgcATCGATAACCTTAGAAAATTTATCGTTATCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCAtt                                                                                                                                       <  4:1207302/149‑1 (MQ=255)
              gcgcATCGATAACCTTAGAAAATTTATCGTTATCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCAtt                                                                                                                                       >  3:1287372/1‑149 (MQ=255)
              gcgcATCGATAACCTTAGAAAATTTATCGTTATCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCAtt                                                                                                                                       <  2:830617/149‑1 (MQ=255)
              gcgcATCGATAACCTTAGAAAATTTATCGTTATCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTGCGGCGTAACTCAtt                                                                                                                                       >  1:337569/1‑149 (MQ=255)
                      aTAACCTTAGAAAATTTATCGTTATCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTc                                                                                                                               >  4:1142226/1‑149 (MQ=255)
                       tAACCTTAGAAAATTTATCGTTATCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCg                                                                                                                              <  1:538844/149‑1 (MQ=255)
                         aCCTTAGAAAATTTATCGTTATCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGgt                                                                                                                            <  3:1419890/149‑1 (MQ=255)
                         aCCTTAGAAAATTTATCGTTATCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGgt                                                                                                                            <  4:793088/149‑1 (MQ=255)
                           cTTAGAAAATTTATCGTTATCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGgtgt                                                                                                                          <  4:925955/149‑1 (MQ=255)
                           cTTAGAAAATTTATCGTTATCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGgtgt                                                                                                                          >  1:1219951/1‑149 (MQ=255)
                            ttAGAAAATTTATCGTTATCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTa                                                                                                                         >  1:451217/1‑149 (MQ=255)
                                    tttATCGTTATCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCgg                                                                                                                 >  3:224402/1‑149 (MQ=255)
                                    tttATCGTTATCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCgg                                                                                                                 <  2:1219951/149‑1 (MQ=255)
                                     ttATCGTTATCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGt                                                                                                                <  2:720461/149‑1 (MQ=255)
                                     ttATCGTTATCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGt                                                                                                                <  4:1402514/149‑1 (MQ=255)
                                           ttATCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCg                                                                                                                  >  4:1247294/1‑141 (MQ=255)
                                           ttATCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATAcc                                                                                                          <  3:551671/149‑1 (MQ=255)
                                              tCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTaa                                                                                                       <  2:335744/149‑1 (MQ=255)
                                               cccAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCAttgttg                                                                                                                                   >  1:672451/1‑120 (MQ=255)
                                               cccAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAAt                                                                                                      >  3:788770/1‑149 (MQ=255)
                                                ccAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATg                                                                                                     <  1:381531/149‑1 (MQ=255)
                                                 cAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATAc                                                                                                           <  4:363960/142‑1 (MQ=255)
                                                 cAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATgg                                                                                                    >  4:862292/1‑149 (MQ=255)
                                                 cAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATgg                                                                                                    >  4:1353197/1‑149 (MQ=255)
                                                  aaCACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGc                                                                                                   >  1:581753/1‑149 (MQ=255)
                                                       ttAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCAttgtt                                                                                                                                    <  2:58370/111‑1 (MQ=255)
                                                       ttAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAg                                                                                              >  1:1148095/1‑149 (MQ=255)
                                                         aaaTATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTGCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGa                                                                                            <  3:61201/149‑1 (MQ=255)
                                                            tatCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCg                                                                                         >  1:504792/1‑149 (MQ=255)
                                                                 gACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAGTTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATaaa                                                                                    <  2:1476154/149‑1 (MQ=255)
                                                                 gACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATaaa                                                                                    <  4:178460/149‑1 (MQ=255)
                                                                     cgcTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTAc                                                                                <  3:1081963/149‑1 (MQ=255)
                                                                            ggTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGAt                                                                                       <  4:40538/135‑1 (MQ=255)
                                                                                ggATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAAt                                                                                                                    <  1:26989/102‑1 (MQ=255)
                                                                                   tGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATAtgt                                                                  <  3:1445457/149‑1 (MQ=255)
                                                                                    gACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGaaa                                                                                                                     <  3:429816/97‑1 (MQ=255)
                                                                                    gACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGaaa                                                                                                                     >  4:429816/1‑97 (MQ=255)
                                                                                    gACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATAc                                                                                                           >  4:743336/1‑107 (MQ=255)
                                                                                    gACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATAc                                                                                                           <  3:743336/107‑1 (MQ=255)
                                                                                    gACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATAtgtg                                                                 >  3:496679/1‑149 (MQ=255)
                                                                                      caacaTAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTa                                                               >  3:911479/1‑149 (MQ=255)
                                                                                       aacaTAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTAc                                                              >  3:205229/1‑149 (MQ=255)
                                                                                       aacaTAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTAc                                                              <  4:679946/149‑1 (MQ=255)
                                                                                       aacaTAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTAc                                                              >  4:256579/1‑149 (MQ=255)
                                                                                          aTAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCgt                                                           <  2:934173/149‑1 (MQ=255)
                                                                                          aTAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCgt                                                           >  1:726033/1‑149 (MQ=255)
                                                                                          aTAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTGCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCgt                                                           <  4:641253/149‑1 (MQ=255)
                                                                                              tGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTAt                                                       >  2:774634/1‑149 (MQ=255)
                                                                                               gATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTAtt                                                      <  1:779737/149‑1 (MQ=255)
                                                                                               gATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTGCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTAtt                                                      >  3:297521/1‑149 (MQ=255)
                                                                                                aTCTTTTACGCCCAGGGTATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATaaa                                                                                    <  2:474281/118‑1 (MQ=255)
                                                                                                 tCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTgg                                                    <  2:1076838/149‑1 (MQ=255)
                                                                                                   ttttACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGgcg                                                  >  3:34461/1‑149 (MQ=255)
                                                                                                   ttttACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGgcg                                                  >  4:1090613/1‑149 (MQ=255)
                                                                                                    tttACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGgcgc                                                 <  1:1435306/149‑1 (MQ=255)
                                                                                                    tttACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGgcgc                                                 <  4:1048586/149‑1 (MQ=255)
                                                                                                         gCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTAAgt                                            >  4:555802/1‑149 (MQ=255)
                                                                                                           ccAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTAAgtgt                                          <  3:72010/149‑1 (MQ=255)
                                                                                                           ccAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTAAgtgt                                          >  2:226622/1‑149 (MQ=255)
                                                                                                             aGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTAAgtgtgt                                        >  1:1192641/1‑149 (MQ=255)
                                                                                                                gCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTAAGTGTGTGaa                                     <  4:14334/149‑1 (MQ=255)
                                                                                                                   tCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTAAGTGTGTGAAGCt                                  >  4:716715/1‑149 (MQ=255)
                                                                                                                   tCTAATCCCCGGCGCATCTTCTGCCAGCAATCTTCGGCGTACATGATTGTTGTGTAAGTGTAGAAATCCGTAATACTTAATGGCTCAAGGACCGATAAATTACTGTTAACCCCACGGGCACCGTGTATTGGCGCAAAGTGTTTTAAGCt                                  <  2:1015945/149‑1 (MQ=255)
                                                                                                                     tAATTCCCGGCGCATCTTCTTCCAGCAATCTGCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATAGCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTAAGTGTGTGAAGCttt                                >  4:1265683/1‑149 (MQ=255)
                                                                                                                     tAATTCCCGGCGCATCATCTTCCAGCAATCTTCGGCGTAACTCATAGTTGTGCCGGTGTAGAAATCGGTAATACCTAATGGCTCaa                                                                                               >  4:222779/1‑86 (MQ=255)
                                                                                                                      aaTTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTAtt                                                      <  2:1365676/126‑1 (MQ=255)
                                                                                                                      aaTTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTAAGTGTGTGAAGCtttt                               <  1:1073554/149‑1 (MQ=255)
                                                                                                                        ttCCCGGCGCATCTTCTTCCAGCAATCTGCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTAAGTGTGTGAAGCtttttt                             <  1:943268/149‑1 (MQ=255)
                                                                                                                         tCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTTGGTGTAGAAATCGGTAATTCCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACTGTGTATTGGCTCTAAATGTGTGAAGAttttttt                            <  2:418616/149‑1 (MQ=255)
                                                                                                                         tCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTAAGTGTGTGAAGCttttttt                            >  2:1158288/1‑149 (MQ=255)
                                                                                                                          cccGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTaa                                              >  4:318025/1‑130 (MQ=255)
                                                                                                                           ccGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTAAGTGTGTGAAGCTTTTTTTcc                          <  2:641303/149‑1 (MQ=255)
                                                                                                                           ccGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTAAGTGTGTGAAGCTTTTTTTcc                          <  3:168429/149‑1 (MQ=255)
                                                                                                                               cgcATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTAAGTGTGTGAAGCTTTTTTTCCAAAt                      <  1:180513/149‑1 (MQ=255)
                                                                                                                                gcATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTAAGTGTGTGAAGCTTTTTTTCCAAATa                     <  1:1028417/149‑1 (MQ=255)
                                                                                                                                 cATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTAAGTGTGTGAAGCTTTTTTTCCAAATAc                    <  3:697977/149‑1 (MQ=255)
                                                                                                                                   tcttctTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAAt                                                                                   <  3:365389/84‑1 (MQ=255)
                                                                                                                                   tcttctTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTAAgtgtgt                                        <  2:868967/127‑1 (MQ=255)
                                                                                                                                   tcttctTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTAAgtgtgt                                        >  1:868967/1‑127 (MQ=255)
                                                                                                                                   tcttctTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTAAGTGTGTGAAGCTTTTTTTCCAAATACCa                  <  3:864635/149‑1 (MQ=255)
                                                                                                                                   tcttctTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTAAGTGTGTGAAGCTTTTTTTCCAAATACCa                  >  1:1276314/1‑149 (MQ=255)
                                                                                                                                        ttcCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAata                                                                     <  1:1339369/93‑1 (MQ=255)
                                                                                                                                            aGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGa                                                                                            >  3:1360/1‑66 (MQ=255)
                                                                                                                                            aGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATAtg                                                                   >  2:979174/1‑91 (MQ=255)
                                                                                                                                            aGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTAAGTGTGTGAAGCTTTTTTTCCaaa                       >  4:596700/1‑135 (MQ=255)
                                                                                                                                             gCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTAAGTGTGTGAAGCTTTTTTTCCAAATACCATGCTGCCGAt        >  3:1252129/1‑149 (MQ=255)
                                                                                                                                               aaTCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTAAgtgtg                                         <  1:1106508/114‑1 (MQ=255)
                                                                                                                                               aaTCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTAAGTGTGTGAAGCTTTTTTTCCAAATACCATGCTGCCGATGa      <  4:483358/149‑1 (MQ=255)
                                                                                                                                               aaTCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTAAGTGTGTGAAGCTTTTTTTCCAAATACCATGCTGCCGATGa      <  1:1392917/149‑1 (MQ=255)
                                                                                                                                                   ttCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTAAGTGTGTGAAGCTTTTTTTCCAAATACCATGCTGCCGATGACCAt  >  2:528995/1‑149 (MQ=255)
                                                                                                                                                   ttCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTAAGTGTGTGAAGCTTTTTTTCCAAATACCATGCTGCCGATGACCAt  <  2:1098361/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCTCGCTGTTGCAGCGCATCGATAACCTTAGAAAATTTATCGTTATCCCAACACTTAAATATCTGACGCGCTGTCGGTTGGATGACAACATAATGATCTTTTACGCCCAGGGCATCTAATTCCCGGCGCATCTTCTTCCAGCAATCTTCGGCGTAACTCATTGTTGTGTCGGTGTAGAAATCGGTAATACCTAATGGCTCAAGGACCGATAAATTACGCTCAACAATATGTGTACCGTGTATTGGCGCTAAGTGTGTGAAGCTTTTTTTCCAAATACCATGCTGCCGATGACCAT  >  NC_000913/3807490‑3807785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: