Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,877,558 G→C 6.9% intergenic (‑93/+147) yidB ← / ← gyrB DUF937 family protein/DNA gyrase, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,877,5580GC 6.9% 454.0 / 18.0 129intergenic (‑93/+147)yidB/gyrBDUF937 family protein/DNA gyrase, subunit B
Reads supporting (aligned to +/- strand):  ref base G (60/60);  new base C (4/5);  total (64/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

TATTTCCCCGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATT  >  NC_000913/3877410‑3877706
                                                                                                                                                    |                                                                                                                                                    
  tttCCCCGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAgatgat                                                                                                                                                    <  4:1285076/149‑1 (MQ=255)
     ccccGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGAtctc                                                                                                                                                 <  1:757102/149‑1 (MQ=255)
     ccccGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGAtctc                                                                                                                                                 <  1:1280991/149‑1 (MQ=255)
      cccGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTcc                                                                                                                                                >  1:820065/1‑149 (MQ=255)
       ccGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAgatgat                                                                                                                                                    <  3:251268/144‑1 (MQ=255)
       ccGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAgatgat                                                                                                                                                    >  4:251268/1‑144 (MQ=255)
         gCATCGCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATCATCTCCGcc                                                                                                                                             <  2:480781/149‑1 (MQ=255)
            tCGCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCtt                                                                                                                                          <  1:256036/149‑1 (MQ=255)
              gCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCtttt                                                                                                                                        >  4:153654/1‑149 (MQ=255)
              gCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCtttt                                                                                                                                        <  2:1297809/149‑1 (MQ=255)
                  ttCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTta                                                                                                                                    <  3:365528/149‑1 (MQ=255)
                    cAGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTtata                                                                                                                                  <  4:595998/149‑1 (MQ=255)
                      gAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCt                                                                                                                                      <  2:855822/143‑1 (MQ=255)
                      gAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGc                                                                                                                                >  4:1160607/1‑149 (MQ=255)
                           gCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGcttctt                                                                                                                           <  3:1424689/149‑1 (MQ=255)
                             aCCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGc                                                                                                                         <  3:1017032/149‑1 (MQ=255)
                              ccGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGcc                                                                                                                        <  1:827276/149‑1 (MQ=255)
                                gACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCgg                                                                                                                      >  3:1390033/1‑149 (MQ=255)
                                 aCAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGa                                                                                                                     >  2:1353407/1‑149 (MQ=255)
                                  cAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGAt                                                                                                                    <  1:301376/149‑1 (MQ=255)
                                   aaCTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATg                                                                                                                   >  3:686828/1‑149 (MQ=255)
                                     cTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGcttc                                                                                                                             <  4:816156/137‑1 (MQ=255)
                                     cTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGcttc                                                                                                                             >  3:816156/1‑137 (MQ=255)
                                      ttCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCtttttattt                                                                                                                                   <  3:1415105/130‑6 (MQ=255)
                                      ttCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCtttttattt                                                                                                                                   >  4:1415105/1‑125 (MQ=255)
                                      ttCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATgctg                                                                                                                <  4:1001636/149‑3 (MQ=255)
                                      ttCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATgcgg                                                                                                                >  1:1311013/1‑149 (MQ=255)
                                      ttCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATgcgg                                                                                                                >  4:780135/1‑149 (MQ=255)
                                         atcaAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTACAGCTTCTTGCCGGATGCGGCGt                                                                                                             >  4:1201016/1‑149 (MQ=255)
                                           caAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGa                                                                                                           <  4:1333205/149‑1 (MQ=255)
                                            aaaaaGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGaa                                                                                                          >  1:234269/1‑149 (MQ=255)
                                             aaaaGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAAc                                                                                                         >  3:1038711/1‑149 (MQ=255)
                                                  ccccATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCtt                                                                                                    >  2:583774/1‑149 (MQ=255)
                                                   cccATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTa                                                                                                   >  3:35555/1‑149 (MQ=255)
                                                    ccATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTAt                                                                                                  >  3:20619/1‑149 (MQ=255)
                                                    ccATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTAt                                                                                                  >  3:501461/1‑149 (MQ=255)
                                                    ccATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTAt                                                                                                  <  1:747602/149‑1 (MQ=255)
                                                    ccATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATc                                                                                                 >  2:1311241/1‑149 (MQ=255)
                                                     cATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATgcgg                                                                                                                >  1:691356/1‑134 (MQ=255)
                                                     cATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATc                                                                                                 <  3:306247/149‑1 (MQ=255)
                                                       tGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTCGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCg                                                                                               >  4:213131/1‑149 (MQ=255)
                                                             tCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAc                                                                                         >  1:1443995/1‑149 (MQ=255)
                                                             tCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAc                                                                                         >  1:579504/1‑149 (MQ=255)
                                                                 cTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCt                                                                                                                                      <  4:180253/100‑1 (MQ=255)
                                                                 cTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCt                                                                                                                                      >  3:180253/1‑100 (MQ=255)
                                                                  tGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAt                                                                                    >  4:1400495/1‑149 (MQ=255)
                                                                  tGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGAGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTta                                                                                                                                    >  3:1049963/1‑101 (MQ=255)
                                                                    tAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCa                                                                                  <  4:686828/149‑1 (MQ=255)
                                                                    tAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCa                                                                                  <  3:153654/149‑1 (MQ=255)
                                                                    tAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCa                                                                                  <  3:1160607/149‑1 (MQ=255)
                                                                     aaaGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCAt                                                                                 <  1:528728/149‑1 (MQ=255)
                                                                      aaGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATg                                                                                >  2:1024334/1‑149 (MQ=255)
                                                                          gTTACGTGTTGACCCAAAAGGTATAGATTTGCGGGATGATAATTGCGGTTTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGaaaa                                                                            <  3:1201016/149‑1 (MQ=255)
                                                                             aCGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTc                                                                         <  4:121807/149‑1 (MQ=255)
                                                                               gtgtTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCaa                                                                       <  1:903127/149‑1 (MQ=255)
                                                                               gtgtTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCaa                                                                       <  1:686734/149‑1 (MQ=255)
                                                                                 gtTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATa                                                                     <  4:501461/149‑1 (MQ=255)
                                                                                  ttGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATAc                                                                    >  1:131341/1‑149 (MQ=255)
                                                                                      cccAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTg                                                                <  2:248561/149‑1 (MQ=255)
                                                                                       ccAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGc                                                               >  4:767997/1‑149 (MQ=255)
                                                                                        cAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTta                                                                                                                                    <  1:536420/79‑1 (MQ=255)
                                                                                        cAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTta                                                                                                                                    >  2:536420/1‑79 (MQ=255)
                                                                                        cAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCa                                                              <  2:131341/149‑1 (MQ=255)
                                                                                        cAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCa                                                              >  1:422000/1‑149 (MQ=255)
                                                                                         aaaaaGTATAGATTTGCGGGATGATAATTTCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATATATTGCaa                                                             >  4:652090/1‑149 (MQ=255)
                                                                                         aaaaaGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCaa                                                             >  3:284861/1‑149 (MQ=255)
                                                                                          aaaaGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAg                                                            >  2:270911/1‑149 (MQ=255)
                                                                                               tataGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGAtttt                                                       >  2:461237/1‑149 (MQ=255)
                                                                                               tataGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCAGGCCTACAAAATCATGAAAATTCAATACATTGCAAGAtttt                                                       >  2:655915/1‑149 (MQ=255)
                                                                                                ataGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACaaaa                                                                                     >  2:244250/1‑118 (MQ=255)
                                                                                                ataGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTc                                                      >  3:255021/1‑149 (MQ=255)
                                                                                                ataGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTc                                                      <  4:1255962/149‑1 (MQ=255)
                                                                                                ataGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTc                                                      <  1:1353407/149‑1 (MQ=255)
                                                                                                  aGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGcc                                                                                                      <  3:1241177/99‑1 (MQ=255)
                                                                                                   gATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGaaa                                                                             >  2:108089/1‑123 (MQ=255)
                                                                                                   gATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGaaa                                                                             <  1:108089/123‑1 (MQ=255)
                                                                                                   gATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGa                                                           <  4:1133807/141‑1 (MQ=255)
                                                                                                   gATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGa                                                           >  3:1133807/1‑141 (MQ=255)
                                                                                                    aTTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAg                                                  >  2:1198329/1‑149 (MQ=255)
                                                                                                    aTTCGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAg                                                  <  3:213131/149‑1 (MQ=255)
                                                                                                     tttGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTc                                                      <  4:577795/144‑1 (MQ=255)
                                                                                                     tttGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTc                                                      >  3:577795/1‑144 (MQ=255)
                                                                                                        gCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCt                                              <  2:820065/149‑1 (MQ=255)
                                                                                                             atgatAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATaa                                         >  3:228460/1‑149 (MQ=255)
                                                                                                                atAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCg                                      >  1:1230604/1‑149 (MQ=255)
                                                                                                                  aaTTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTa                                    <  1:1198329/149‑1 (MQ=255)
                                                                                                                    ttGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAgc                                  <  3:283325/149‑1 (MQ=255)
                                                                                                                        ggATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCAt                              >  4:1146313/1‑149 (MQ=255)
                                                                                                                             gCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGc                         >  4:121101/1‑149 (MQ=255)
                                                                                                                                  taagaaaaGGGCGGAGATCATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAATGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGCAGGCCTGATAAGCGTAGCGCATCAGGCACGCt                    >  3:911024/5‑149 (MQ=255)
                                                                                                                                  aaTAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCt                    <  3:767997/149‑1 (MQ=255)
                                                                                                                                   aTAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTc                   <  4:20619/149‑1 (MQ=255)
                                                                                                                                        aaGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATg              <  1:1024334/149‑1 (MQ=255)
                                                                                                                                          gggCGGAGATCATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGt            >  1:1016447/1‑149 (MQ=255)
                                                                                                                                           ggCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGtt           >  1:218675/1‑149 (MQ=255)
                                                                                                                                           ggCGGAGATCATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGtt           >  2:742641/1‑149 (MQ=255)
                                                                                                                                            gCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTa          >  2:396623/1‑149 (MQ=255)
                                                                                                                                              ggAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAgc        <  4:35555/149‑1 (MQ=255)
                                                                                                                                               gagaTGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAgcg       >  2:1220875/1‑149 (MQ=255)
                                                                                                                                                agaTGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAgcgc      >  4:714217/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TATTTCCCCGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGCCCTTTTCTTATAGCTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCACGCTCGCATGGTTAGCGCCATT  >  NC_000913/3877410‑3877706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: