Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,882,515 A→C 5.1% R405R (CGT→CGG)  dnaA ← chromosomal replication initiator protein DnaA, DNA‑binding transcriptional dual regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,882,5150AC 5.1% 556.7 / 17.6 157R405R (CGT→CGG) dnaAchromosomal replication initiator protein DnaA, DNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base A (76/73);  new base C (3/5);  total (79/78)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

Only 100 of 158 total aligned reads displayed.

ATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTCCTGCAATGCCAGCAAGTCGCGCAGCGCCTCACGCACGAAGTCGA  >  NC_000913/3882369‑3882663
                                                                                                                                                  |                                                                                                                                                    
   tCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACgcgc                                                                                                                                                 >  3:868474/1‑149 (MQ=255)
       ggCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGTCCGCGCCaga                                                                                                                                             <  4:262712/149‑3 (MQ=255)
         ctctctTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGTCCGCGCCagatg                                                                                                                                           <  1:1090486/149‑5 (MQ=255)
         ctctctTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGa                                                                                                                                           >  1:1187683/1‑149 (MQ=255)
              ttCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCggg                                                                                                                                      >  1:910553/1‑149 (MQ=255)
              ttCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCg                                                                                                                                        <  4:536877/147‑1 (MQ=255)
              ttCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCg                                                                                                                                        >  3:536877/1‑147 (MQ=255)
                cACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGAt                                                                                                                                    <  4:868474/149‑1 (MQ=255)
                cACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGAt                                                                                                                                    <  2:1202890/149‑1 (MQ=255)
                cACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGAt                                                                                                                                    <  3:154716/149‑1 (MQ=255)
                 aCGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATc                                                                                                                                   <  1:24299/149‑1 (MQ=255)
                 aCGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATc                                                                                                                                   >  4:605811/1‑149 (MQ=255)
                 aCGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATc                                                                                                                                   <  3:1228763/149‑1 (MQ=255)
                        tGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCtt                                                                                                                            <  3:779428/149‑1 (MQ=255)
                           tCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGa                                                                                                                         >  4:814181/1‑149 (MQ=255)
                           tCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGa                                                                                                                         >  4:1453587/1‑149 (MQ=255)
                             gATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGaaa                                                                                                                       <  4:459717/149‑1 (MQ=255)
                             gATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGaaa                                                                                                                       >  2:768820/1‑149 (MQ=255)
                              aTCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAg                                                                                                                      >  2:224383/1‑149 (MQ=255)
                              aTCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAg                                                                                                                      <  3:527597/149‑1 (MQ=255)
                                cTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGga                                                                                                                    <  1:926453/149‑1 (MQ=255)
                                cTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGga                                                                                                                    <  2:47390/149‑1 (MQ=255)
                                cTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGga                                                                                                                    <  2:49307/149‑1 (MQ=255)
                                 ttACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGgag                                                                                                                   >  4:491023/1‑149 (MQ=255)
                                 ttACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGgag                                                                                                                   >  3:807942/1‑149 (MQ=255)
                                  tACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGAt                                                                                                                                    >  3:505311/1‑131 (MQ=255)
                                  tACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGgaga                                                                                                                  <  4:886739/149‑1 (MQ=255)
                                     ggcaggcaTGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATcc                                                                                                               >  3:642317/1‑149 (MQ=255)
                                              gAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGTCCGCGCCagat                                                                                                                                            <  3:926780/111‑4 (MQ=255)
                                              gAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTg                                                                                                      >  1:594846/1‑149 (MQ=255)
                                              gAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTg                                                                                                      >  1:1236916/1‑149 (MQ=255)
                                                aGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGAt                                                                                                    >  2:132994/1‑149 (MQ=255)
                                                aGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGAt                                                                                                    >  1:250047/1‑149 (MQ=255)
                                                 gCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATc                                                                                                   <  2:1104769/149‑1 (MQ=255)
                                                 gCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATc                                                                                                   >  3:797441/1‑149 (MQ=255)
                                                   aCCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCtt                                                                                                 <  2:1252006/149‑1 (MQ=255)
                                                     cgtcgtGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTgt                                                                                               <  2:149209/149‑1 (MQ=255)
                                                      gtcgtGTGGTCACGGCCACCAATCGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGAACGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTgta                                                                                              <  4:1202741/149‑1 (MQ=255)
                                                      gtcgtGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTgta                                                                                              <  4:452612/149‑1 (MQ=255)
                                                         gtgtGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTgtagta                                                                                           >  4:7200/1‑149 (MQ=255)
                                                          tgtgGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTAc                                                                                          >  1:50483/1‑149 (MQ=255)
                                                             ggTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTcc                                                                                       <  4:185754/149‑1 (MQ=255)
                                                               tCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGc                                                                                     >  1:102960/1‑149 (MQ=255)
                                                               tCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGc                                                                                     >  3:414768/1‑149 (MQ=255)
                                                                   ggCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCAcc                                                                                 >  1:698145/1‑149 (MQ=255)
                                                                          aaaCGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGTGGGATCGACGCTTGGAAAGGAGATCCGCGACCTTGATCTTGTAGTACTCCGCCACCGtcttct                                                                          <  4:396764/149‑1 (MQ=255)
                                                                          aaaCGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGtcttct                                                                          <  4:1029363/149‑1 (MQ=255)
                                                                             cGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTGCTGaa                                                                       <  2:997498/149‑1 (MQ=255)
                                                                               cATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAata                                                                     <  3:686069/149‑1 (MQ=255)
                                                                                aTCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAatat                                                                    >  2:708992/1‑149 (MQ=255)
                                                                                aTCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAAATCCGCGGCTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAatat                                                                    >  2:1262868/1‑149 (MQ=255)
                                                                                   gCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGTACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGt                                                                 >  4:535741/1‑149 (MQ=255)
                                                                                   gCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGt                                                                 >  1:168398/1‑149 (MQ=255)
                                                                                   gCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGt                                                                 >  2:189818/1‑149 (MQ=255)
                                                                                   gCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGt                                                                 <  3:743999/149‑1 (MQ=255)
                                                                                    ccAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATc                                                                                                   >  3:885344/1‑114 (MQ=255)
                                                                                      aaTCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTgta                                                                                              >  2:272475/1‑117 (MQ=255)
                                                                                      aaTCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGa                                                              <  2:259386/149‑1 (MQ=255)
                                                                                          tcCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGt                                                          >  3:1064956/1‑149 (MQ=255)
                                                                                          tcCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGt                                                          <  1:946937/149‑1 (MQ=255)
                                                                                            cGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGa                                                        <  4:1256427/149‑1 (MQ=255)
                                                                                             ggCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGAc                                                       >  2:1396651/1‑149 (MQ=255)
                                                                                                    tgtgGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGtttt                                                <  4:1064956/149‑1 (MQ=255)
                                                                                                      tgGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTc                                              <  3:212549/149‑1 (MQ=255)
                                                                                                      tgGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTc                                              <  2:739867/149‑1 (MQ=255)
                                                                                                      tgGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTc                                              <  2:797319/149‑1 (MQ=255)
                                                                                                      tgGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTc                                              <  1:15187/149‑1 (MQ=255)
                                                                                                      tgGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCGTCTGAATATTGTCGATGGTGACCAGTTTTTc                                              >  4:156026/1‑149 (MQ=255)
                                                                                                       ggTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTcc                                             <  3:1453587/149‑1 (MQ=255)
                                                                                                       ggTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTcc                                             <  2:386768/149‑1 (MQ=255)
                                                                                                        gTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCg                                                                                                                                        <  3:771992/57‑1 (MQ=255)
                                                                                                        gTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCg                                                                                                                                        >  4:771992/1‑57 (MQ=255)
                                                                                                        gTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAatat                                                                    <  4:1400251/125‑1 (MQ=255)
                                                                                                        gTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAatat                                                                    >  3:1400251/1‑125 (MQ=255)
                                                                                                        gTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTCCt                                            >  3:370040/1‑149 (MQ=255)
                                                                                                        gTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTCCt                                            >  4:1046961/1‑149 (MQ=255)
                                                                                                             tCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTCCTGCAAt                                       >  1:530534/1‑149 (MQ=255)
                                                                                                             tCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTCCTGCAAt                                       <  2:16079/149‑1 (MQ=255)
                                                                                                               aGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGtcttc                                                                           <  2:852524/111‑1 (MQ=255)
                                                                                                               aGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGtcttc                                                                           >  1:852524/1‑111 (MQ=255)
                                                                                                               aGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTCCTGCAAtcc                                     >  1:813143/1‑147 (MQ=255)
                                                                                                                gCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTCCTGCAATGcc                                    >  1:1035167/1‑149 (MQ=255)
                                                                                                                 ctctTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTCCTGCAATGCCa                                   >  1:940015/1‑149 (MQ=255)
                                                                                                                  tctTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTCCTGCAATGCCAg                                  >  2:1308762/1‑149 (MQ=255)
                                                                                                                          cAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTCCTGCAATGCCAGCAAGTcgc                          >  1:362111/1‑149 (MQ=255)
                                                                                                                                catcgccatcTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTCCTGCAATGCCAGCAAGTCGCGCAgcg                    >  4:1138572/1‑149 (MQ=255)
                                                                                                                                  tcgccatcTGGCGCGGACGCGCCCCCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTCCTGCAATGCCAGCAAGTCGCGCAGCGcc                  >  2:948925/1‑149 (MQ=255)
                                                                                                                                    gccatcTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTCCTGCAATGCCAGCAAGTCGCGCAGCGCCTc                <  1:1053775/149‑1 (MQ=255)
                                                                                                                                    gccatcTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTCCTGCAATGCCAGCAAGTCGCGCAGCGCCTc                <  2:937775/149‑1 (MQ=255)
                                                                                                                                     ccatcTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTCCTGCAATGCCa                                   >  2:189857/1‑129 (MQ=255)
                                                                                                                                     ccatcTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTCCTGCAATGCCa                                   <  1:189857/129‑1 (MQ=255)
                                                                                                                                      tcggtGGCGCGTCCGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTCCTGCAATGCCAGCAAGTCGCGCAGCGCCTcac              >  1:287099/5‑149 (MQ=255)
                                                                                                                                      catcTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTCCTGCAATGCCAGCAAGTCGCGCAGCGCCTcac              >  2:118838/1‑149 (MQ=255)
                                                                                                                                       atcTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATgg                                                           <  3:1331947/103‑1 (MQ=255)
                                                                                                                                       atcTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTCCTGCGATGCCAGCAAGTCGCGCAGCGCCTcacg             >  2:41038/1‑149 (MQ=255)
                                                                                                                                       atcTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTCCTGCAATGCCAGCAAGTCGCGCAGCGCCTcacg             <  4:452730/149‑1 (MQ=255)
                                                                                                                                         cTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTCCTGCAATGCCAGCAAGTCGCGCAGCGCCTcacgca           <  1:69843/149‑1 (MQ=255)
                                                                                                                                          tGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTCCTGCAATGCCAGCAAGTCGCGCAGCGCCTcacgcac          >  2:874748/1‑149 (MQ=255)
                                                                                                                                             cgcgGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTCCTGCAATGCCAGCAAGTCGCGCAGCGCCTCACGCACGaa       <  3:459227/149‑1 (MQ=255)
                                                                                                                                              gcgGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTCCTGCAATGCCAGCAAGTCGCGCAGCGCCTCACGCACGAAg      >  1:714280/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
ATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGATCGACGCTTGGAAAGGAGATCCGCGACTTTGATCTTGTAGTACTCCGCCACCGTCTTCTGAATATTGTCGATGGTGACCAGTTTTTCCTGCAATGCCAGCAAGTCGCGCAGCGCCTCACGCACGAAGTCGA  >  NC_000913/3882369‑3882663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑