Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,395,455 G→A 5.2% A464T (GCA→ACA)  nnr → bifunctional NAD(P)H‑hydrate repair enzyme; C‑terminal domain ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase and N‑terminal domain NAD(P)H‑hydrate epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,395,4550GA 5.2% 448.0 / 10.2 135A464T (GCA→ACA) nnrbifunctional NAD(P)H‑hydrate repair enzyme; C‑terminal domain ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase and N‑terminal domain NAD(P)H‑hydrate epimerase
Reads supporting (aligned to +/- strand):  ref base G (57/71);  new base A (5/2);  total (62/73)
Fisher's exact test for biased strand distribution p-value = 2.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.71e-01

Only 100 of 140 total aligned reads displayed.

CGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAAT  >  NC_000913/4395307‑4395601
                                                                                                                                                    |                                                                                                                                                  
 ggAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGc                                                                                                                                                   >  4:163319/1‑149 (MQ=255)
     ccGTGGTCGCCGCCCACCCTGACGGTTTAGGCATTATTGTTGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCACCCTGTGCAGGc                                                                                                                                               <  3:1280086/149‑1 (MQ=255)
       gTGGTCGCCCCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCtg                                                                                                                                             >  2:976951/1‑149 (MQ=255)
        tGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCtgt                                                                                                                                            <  4:100172/149‑1 (MQ=255)
           tCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTc                                                                                                                                         <  4:1263921/149‑1 (MQ=255)
             gccgccCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTcgc                                                                                                                                       <  3:316774/149‑1 (MQ=255)
             gccgccCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTcgc                                                                                                                                       >  2:30334/1‑149 (MQ=255)
             gccgccCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCCGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTcgc                                                                                                                                       <  3:1015041/149‑1 (MQ=255)
                      ccTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCa                                                                                                                                    <  1:1062770/143‑1 (MQ=255)
                      ccTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCa                                                                                                                                    >  2:1062770/1‑143 (MQ=255)
                       cTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCg                                                                                                                             <  4:910906/149‑1 (MQ=255)
                        tGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCgg                                                                                                                            <  1:578801/149‑1 (MQ=255)
                         gACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGc                                                                                                                           >  3:699033/1‑149 (MQ=255)
                             cTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCt                                                                                                                       <  2:189380/149‑1 (MQ=255)
                              tttAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGAGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTg                                                                                                                      >  1:1000295/1‑149 (MQ=255)
                               ttAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGa                                                                                                                     >  2:72029/1‑149 (MQ=255)
                                tAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGAc                                                                                                                    <  2:563118/149‑1 (MQ=255)
                                tAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGAc                                                                                                                    <  3:86118/149‑1 (MQ=255)
                                   gCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTa                                                                                                                 >  3:413957/1‑149 (MQ=255)
                                   gCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTa                                                                                                                 <  3:649204/149‑1 (MQ=255)
                                   gCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTa                                                                                                                 >  3:157497/1‑149 (MQ=255)
                                   gCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTa                                                                                                                 >  4:1249230/1‑149 (MQ=255)
                                    cATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTAc                                                                                                                <  1:194864/149‑1 (MQ=255)
                                       tattGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGCGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTgg                                                                                                             <  1:309894/149‑1 (MQ=255)
                                        attGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTggc                                                                                                            <  1:688787/149‑1 (MQ=255)
                                                cGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGtt                                                                                                    >  4:1375252/1‑149 (MQ=255)
                                                 ggAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGttt                                                                                                   >  4:1035670/1‑149 (MQ=255)
                                                 ggAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGttt                                                                                                   <  2:1339731/149‑1 (MQ=255)
                                                  gAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGtttt                                                                                                  <  4:157497/149‑1 (MQ=255)
                                                  gAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGtt                                                                                                    <  1:1215112/147‑1 (MQ=255)
                                                  gAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGtt                                                                                                    >  2:1215112/1‑147 (MQ=255)
                                                   aaaTGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTg                                                                                                 <  1:407533/149‑1 (MQ=255)
                                                      tGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGaa                                                                                              >  1:62287/1‑149 (MQ=255)
                                                        cAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAAcg                                                                                            >  3:1121371/1‑149 (MQ=255)
                                                        cAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAAcg                                                                                            >  4:854450/1‑149 (MQ=255)
                                                         aGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAAcgc                                                                                           >  4:15170/1‑149 (MQ=255)
                                                         aGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAAcgc                                                                                           >  4:835624/1‑149 (MQ=255)
                                                           gCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAAcgcgc                                                                                         >  1:671114/1‑149 (MQ=255)
                                                           gCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAAcgcgc                                                                                         <  4:2557/149‑1 (MQ=255)
                                                             aTGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCgg                                                                                       <  2:294797/149‑1 (MQ=255)
                                                               ggCGAGCGGCGGCATGGGCGATGTGCGCTCTGGTATTATTGGCGCATTGCTGGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCTGCAGCTGCCGTACTTGCGGCGCGGTTTGGAACGCGCCGGa                                                                                     >  2:1420142/1‑149 (MQ=255)
                                                                  gAGCGGCGGCATGGGCGATGTGCCCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGc                                                                                  <  2:542082/149‑1 (MQ=255)
                                                                          gcATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAAcc                                                                          >  2:54355/1‑149 (MQ=255)
                                                                             tGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGAt                                                                       <  1:762868/149‑1 (MQ=255)
                                                                             tGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGAt                                                                       <  2:317756/149‑1 (MQ=255)
                                                                              gggCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGAtc                                                                      <  2:197017/149‑1 (MQ=255)
                                                                              gggCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGAtc                                                                      >  3:364779/1‑149 (MQ=255)
                                                                                 cGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATctct                                                                   <  1:122480/149‑1 (MQ=255)
                                                                                  gATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCtt                                                                  >  2:805813/1‑149 (MQ=255)
                                                                                      tgCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTcc                                                              >  1:511577/1‑149 (MQ=255)
                                                                                      tgCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTcc                                                              <  3:1417827/149‑1 (MQ=255)
                                                                                       gCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCa                                                             <  2:899162/149‑1 (MQ=255)
                                                                                          ctctGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTGGCGGTATGATGCAGCCTGTGCAGGCTGAGTCACGCGCGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACTCGCGGGATGCTGGCAACCGATCTCTTGTCcgcgg                                                          <  2:1172487/149‑2 (MQ=255)
                                                                                           tctGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCt                                                         >  2:1285648/1‑149 (MQ=255)
                                                                                           tctGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCt                                                         >  3:458098/1‑149 (MQ=255)
                                                                                             tGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTAc                                                       >  4:324752/1‑149 (MQ=255)
                                                                                              ggTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACa                                                      >  1:272119/1‑149 (MQ=255)
                                                                                                tattatTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGc                                                    <  3:324752/149‑1 (MQ=255)
                                                                                                tattatTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGc                                                    >  3:1162450/1‑149 (MQ=255)
                                                                                                  ttattGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGt                                                  >  1:1310007/1‑149 (MQ=255)
                                                                                                  ttattGGCGCATTGCATGGGCAAAAACTGTAGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGACAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGt                                                  >  4:1221386/1‑149 (MQ=255)
                                                                                                        gcgcATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGtt                                            <  3:1217776/149‑1 (MQ=255)
                                                                                                          gcATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTaa                                          <  4:699033/149‑1 (MQ=255)
                                                                                                          gcATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTaa                                          >  3:382838/1‑149 (MQ=255)
                                                                                                             ttGCTTGGGCAAAAACTGTCGCCGTATGTTGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAAccc                                       <  1:141772/149‑1 (MQ=255)
                                                                                                             ttGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAAccc                                       <  3:7167/149‑1 (MQ=255)
                                                                                                                   gggCAAAATCTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGt                                 <  1:1420142/149‑1 (MQ=255)
                                                                                                                     gCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGa                               <  3:835624/149‑1 (MQ=255)
                                                                                                                     gCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGa                               >  4:463927/1‑149 (MQ=255)
                                                                                                                       aaaaaCTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACt                             >  2:1355220/1‑149 (MQ=255)
                                                                                                                        aaaaCTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTg                            >  1:470116/1‑149 (MQ=255)
                                                                                                                         aaaCTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGa                           >  2:605078/1‑149 (MQ=255)
                                                                                                                           aCTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATa                         >  4:953425/1‑149 (MQ=255)
                                                                                                                             tGTCGCCGTATTATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATaaa                       >  4:445792/1‑149 (MQ=255)
                                                                                                                               tCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATaaaaa                     <  1:605078/149‑1 (MQ=255)
                                                                                                                               tCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATaaaaa                     <  4:1162450/149‑1 (MQ=255)
                                                                                                                               tCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATaaaaa                     <  4:382838/149‑1 (MQ=255)
                                                                                                                               tCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATaaaaa                     <  1:979393/149‑1 (MQ=255)
                                                                                                                                cGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAAc                    <  1:722351/149‑1 (MQ=255)
                                                                                                                                 gCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGTTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAAcc                   <  3:172655/149‑1 (MQ=255)
                                                                                                                                  ccGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCa                  <  1:742073/149‑1 (MQ=255)
                                                                                                                                      gcgacacAGCCTGCACAGGCTGCGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCat                 >  2:295680/7‑146 (MQ=255)
                                                                                                                                      atgatgCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCatgat              <  3:993196/149‑1 (MQ=255)
                                                                                                                                        gacacAGCCTGCACAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCAtgatga            >  2:710368/5‑149 (MQ=255)
                                                                                                                                         acacAGCCTGCACAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGaa           >  2:401824/4‑149 (MQ=255)
                                                                                                                                         acacAGCCTGCACAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGaa           >  4:184648/4‑149 (MQ=255)
                                                                                                                                          cacAGCCTGCACAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAAt          >  3:1015938/3‑149 (MQ=255)
                                                                                                                                           gCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATc         <  1:904635/149‑1 (MQ=255)
                                                                                                                                           gCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATc         <  3:163319/149‑1 (MQ=255)
                                                                                                                                           gCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATc         >  1:317426/1‑149 (MQ=255)
                                                                                                                                             aGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGa       <  2:313108/149‑1 (MQ=255)
                                                                                                                                             aGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGa       >  4:1430404/1‑149 (MQ=255)
                                                                                                                                             aGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGa       <  4:1297182/149‑1 (MQ=255)
                                                                                                                                             aGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGa       <  3:1035670/149‑1 (MQ=255)
                                                                                                                                             aGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGa       <  3:1082442/149‑1 (MQ=255)
                                                                                                                                             aGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGa       <  3:1375252/149‑1 (MQ=255)
                                                                                                                                              gCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGACCGCGCGGGATGCTGGCAACCGATCTCTTTTCCGCGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAATCCATGATGAACCGAg      <  4:1076247/149‑1 (MQ=255)
                                                                                                                                                 tgtgCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATaaaaa                     <  2:1002807/131‑1 (MQ=255)
                                                                                                                                                 tgtgCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATaaaaa                     >  1:1002807/1‑131 (MQ=255)
                                                                                                                                                  gtgCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAAt  >  3:208848/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAAT  >  NC_000913/4395307‑4395601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: