Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,537,639 T→G 5.2% intergenic (‑45/+20) nanS ← / ← nanM 9‑O‑acetyl N‑acetylneuraminic acid esterase/N‑acetylneuraminic acid mutarotase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,537,6390TG 5.2% 470.9 / 15.5 134intergenic (‑45/+20)nanS/nanM9‑O‑acetyl N‑acetylneuraminic acid esterase/N‑acetylneuraminic acid mutarotase
Reads supporting (aligned to +/- strand):  ref base T (59/68);  new base G (5/2);  total (64/70)
Fisher's exact test for biased strand distribution p-value = 2.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.99e-01

Only 100 of 138 total aligned reads displayed.

TCTTCCCTGTCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGATACTCCGTAGG  >  NC_000913/4537491‑4537786
                                                                                                                                                    |                                                                                                                                                   
tCTTCCCTGTCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCt                                                                                                                                                     >  2:1340335/1‑149 (MQ=255)
  ttCCCTGTCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCttt                                                                                                                                                   >  1:1273796/1‑149 (MQ=255)
  ttCCCTGTCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCttt                                                                                                                                                   >  1:1458055/1‑149 (MQ=255)
   tCCCTGTCCGGGAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCTACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTa                                                                                                                                                  >  4:135927/1‑149 (MQ=255)
    cccTGTCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTAc                                                                                                                                                 >  3:889723/1‑149 (MQ=255)
      cTGTCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCg                                                                                                                                               >  3:782475/1‑149 (MQ=255)
       tGTCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCgg                                                                                                                                              >  3:948453/1‑149 (MQ=255)
        gTCCGGTAATGGCAGTCCTTCGTCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCggg                                                                                                                                             <  3:894904/149‑1 (MQ=255)
         tCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCgggg                                                                                                                                            <  2:207336/149‑1 (MQ=255)
         tCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCgggg                                                                                                                                            <  4:171450/149‑1 (MQ=255)
         tCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCgggg                                                                                                                                            >  2:858191/1‑149 (MQ=255)
         tCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCgggg                                                                                                                                            <  1:1424727/149‑1 (MQ=255)
          ccGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGc                                                                                                                                           <  1:175478/149‑1 (MQ=255)
          ccGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGc                                                                                                                                           <  2:918679/149‑1 (MQ=255)
           cGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGcc                                                                                                                                          >  1:608344/1‑149 (MQ=255)
             gTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCt                                                                                                                                        <  1:643317/149‑1 (MQ=255)
                aTGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGaa                                                                                                                                     <  4:697697/149‑1 (MQ=255)
                aTGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGaa                                                                                                                                     <  2:1330333/149‑1 (MQ=255)
                aTGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGaa                                                                                                                                     <  4:782475/149‑1 (MQ=255)
                 tGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAg                                                                                                                                    <  2:1208790/149‑1 (MQ=255)
                   gCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCggg                                                                                                                                             >  4:799456/1‑138 (MQ=255)
                   gCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCggg                                                                                                                                             <  3:799456/138‑1 (MQ=255)
                   gCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCg                                                                                                                                  >  2:581836/1‑149 (MQ=255)
                    cAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGt                                                                                                                                 <  4:258799/149‑1 (MQ=255)
                    cAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGt                                                                                                                                 <  3:1455123/149‑1 (MQ=255)
                    cAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGt                                                                                                                                 >  3:941769/1‑149 (MQ=255)
                     aGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGtt                                                                                                                                <  1:520736/149‑1 (MQ=255)
                      gTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTa                                                                                                                               <  2:1449942/149‑1 (MQ=255)
                         cTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGtt                                                                                                                            >  1:123998/1‑149 (MQ=255)
                          ttCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGttt                                                                                                                           >  2:845595/1‑149 (MQ=255)
                          ttCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGttt                                                                                                                           <  1:1104472/149‑1 (MQ=255)
                           tCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGtttt                                                                                                                          <  4:364212/149‑1 (MQ=255)
                                aTACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACt                                                                                                                     <  2:123998/149‑1 (MQ=255)
                                 tACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGtttt                                                                                                                          >  2:1266364/1‑143 (MQ=255)
                                  aCGCCATGGCATTAGACTGACAAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACtgt                                                                                                                   <  3:352347/149‑1 (MQ=255)
                                    gCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGa                                                                                                                 >  3:178297/1‑149 (MQ=255)
                                     ccATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGAc                                                                                                                <  1:2902/149‑1 (MQ=255)
                                       aTGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACtt                                                                                                              >  1:1234706/1‑149 (MQ=255)
                                        tGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACttt                                                                                                             >  4:196260/1‑149 (MQ=255)
                                         ggCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGtttt                                                                                                                          >  4:442267/1‑135 (MQ=255)
                                         ggCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGtttt                                                                                                                          <  3:442267/135‑1 (MQ=255)
                                            aTTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTttatta                                                                                                         >  2:168929/1‑149 (MQ=255)
                                             ttAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCTTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTa                                                                                                                       <  2:411769/134‑1 (MQ=255)
                                              tAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATc                                                                                                       <  3:202357/149‑1 (MQ=255)
                                                gACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCt                                                                                                     >  2:547995/1‑149 (MQ=255)
                                                  cTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGcc                                                                                                                                          >  4:889036/1‑110 (MQ=255)
                                                     aCCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTcaca                                                                                                <  2:1271075/149‑1 (MQ=255)
                                                         gCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGa                                                                                            <  2:470061/149‑1 (MQ=255)
                                                           aaCGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCTCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATc                                                                                          >  3:1200536/1‑149 (MQ=255)
                                                               gTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCaaaa                                                                                      <  1:1368129/149‑1 (MQ=255)
                                                                   gAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTACTTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTg                                                                                             <  4:1213410/138‑1 (MQ=255)
                                                                   gAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTg                                                                                             >  3:1213410/1‑138 (MQ=255)
                                                                       aTAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGtt                                                                                                                            <  4:1252618/103‑1 (MQ=255)
                                                                         aGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGt                                                                            <  2:733436/149‑1 (MQ=255)
                                                                           taataaTCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACt                                                                                                                     >  2:512060/1‑106 (MQ=255)
                                                                           taataaTCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACt                                                                                                                     <  1:512060/106‑1 (MQ=255)
                                                                            aataatCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCAc                                                                         <  2:1153085/149‑1 (MQ=255)
                                                                            aataatCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCAc                                                                         >  3:862521/1‑149 (MQ=255)
                                                                            aataatCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCAc                                                                         >  1:352491/1‑149 (MQ=255)
                                                                             ataatCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCGGTAAAGGGGCCCTgct                                                                                                                                     <  1:162273/88‑3 (MQ=38)
                                                                             ataatCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCGGTAAAGGGGCCCTgct                                                                                                                                     >  2:162273/1‑86 (MQ=37)
                                                                                    ggCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTgc                                                                 >  3:1134557/1‑149 (MQ=255)
                                                                                       gATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTgccgc                                                              >  4:649900/1‑149 (MQ=255)
                                                                                        atatTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTgccgcc                                                             <  1:845595/149‑1 (MQ=255)
                                                                                                 cGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTc                                                    <  4:1239846/149‑1 (MQ=255)
                                                                                                  gTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCa                                                   >  1:298838/1‑149 (MQ=255)
                                                                                                    tCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTg                                                                                                                         >  4:2523/1‑77 (MQ=255)
                                                                                                             tttCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGtt                                                                                                                                <  2:88392/61‑1 (MQ=255)
                                                                                                             tttCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGtt                                                                                                                                >  1:88392/1‑61 (MQ=255)
                                                                                                             tttCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCaataat                                        <  4:889723/149‑1 (MQ=255)
                                                                                                                 gcgTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAAt                                    >  1:1289461/1‑149 (MQ=255)
                                                                                                                  cgTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATa                                   >  1:822875/1‑149 (MQ=255)
                                                                                                                   gTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGaa                                                                                 <  3:551743/102‑1 (MQ=255)
                                                                                                                   gTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAg                                  >  2:931775/1‑149 (MQ=255)
                                                                                                                    tAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGa                                 <  1:1340335/149‑1 (MQ=255)
                                                                                                                     aaTTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGAc                                <  2:608344/149‑1 (MQ=255)
                                                                                                                      aTTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACt                               >  1:740051/1‑149 (MQ=255)
                                                                                                                       ttGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACta                              >  1:333681/1‑149 (MQ=255)
                                                                                                                       ttGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACta                              >  4:322239/1‑149 (MQ=255)
                                                                                                                          aaaTGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACtatta                           >  4:616323/1‑149 (MQ=255)
                                                                                                                           aaTGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACtattat                          >  3:126833/1‑149 (MQ=255)
                                                                                                                            aTGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTattatt                         >  3:1430894/1‑149 (MQ=255)
                                                                                                                               aCAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCAcc                                                 >  2:812187/1‑122 (MQ=255)
                                                                                                                               aCAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCAcc                                                 <  1:812187/122‑1 (MQ=255)
                                                                                                                                 aaaTTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCaataa                                         <  2:1448144/128‑1 (MQ=255)
                                                                                                                                 aaaTTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAgg                    <  2:740051/149‑1 (MQ=255)
                                                                                                                                 aaaTTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAgg                    <  3:322239/149‑1 (MQ=255)
                                                                                                                                 aaaTTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAgg                    <  2:1289461/149‑1 (MQ=255)
                                                                                                                                    ttGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCa                 >  2:300085/1‑149 (MQ=255)
                                                                                                                                     tGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCaa                <  1:986410/149‑1 (MQ=255)
                                                                                                                                        tAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGa             <  3:649900/149‑1 (MQ=255)
                                                                                                                                        cttcAGGGCCCCGGTAAAGGGGCCCTGCAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGa             >  3:1178277/4‑149 (MQ=255)
                                                                                                                                         ttcAGGGCCCCGGTAAAGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGAt            >  2:277803/3‑149 (MQ=255)
                                                                                                                                         ttcAGGGCCCCGGTAAAGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGAt            >  3:1255660/3‑149 (MQ=255)
                                                                                                                                         ttcAGGGCCCCGGTAAAGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGAt            >  1:317948/3‑149 (MQ=255)
                                                                                                                                         aGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGAt            >  3:1337807/1‑149 (MQ=255)
                                                                                                                                              ggCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAACCTGAATCCGTCACCGCTTTg                                                                  <  4:1039650/90‑1 (MQ=255)
                                                                                                                                              ggCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTg                                                                  >  3:1039650/1‑90 (MQ=255)
                                                                                                                                                   cTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGATACTCCGTAgg  >  3:1264602/1‑149 (MQ=255)
                                                                                                                                                   cTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGATACTCCGTAgg  >  1:48855/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCTTCCCTGTCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGATACTCCGTAGG  >  NC_000913/4537491‑4537786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: