Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,537,640 T→G 5.2% intergenic (‑46/+19) nanS ← / ← nanM 9‑O‑acetyl N‑acetylneuraminic acid esterase/N‑acetylneuraminic acid mutarotase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,537,6400TG 5.2% 477.3 / 15.7 134intergenic (‑46/+19)nanS/nanM9‑O‑acetyl N‑acetylneuraminic acid esterase/N‑acetylneuraminic acid mutarotase
Reads supporting (aligned to +/- strand):  ref base T (59/68);  new base G (5/2);  total (64/70)
Fisher's exact test for biased strand distribution p-value = 2.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.75e-01

Only 100 of 137 total aligned reads displayed.

CTTCCCTGTCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGATACTCCGTAGG  >  NC_000913/4537492‑4537786
                                                                                                                                                    |                                                                                                                                                  
 ttCCCTGTCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCttt                                                                                                                                                   >  1:1273796/1‑149 (MQ=255)
  tCCCTGTCCGGGAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCTACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTa                                                                                                                                                  >  4:135927/1‑149 (MQ=255)
   cccTGTCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTAc                                                                                                                                                 >  3:889723/1‑149 (MQ=255)
     cTGTCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCg                                                                                                                                               >  3:782475/1‑149 (MQ=255)
      tGTCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCgg                                                                                                                                              >  3:948453/1‑149 (MQ=255)
       gTCCGGTAATGGCAGTCCTTCGTCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCggg                                                                                                                                             <  3:894904/149‑1 (MQ=255)
        tCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCgggg                                                                                                                                            <  4:171450/149‑1 (MQ=255)
        tCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCgggg                                                                                                                                            <  1:1424727/149‑1 (MQ=255)
        tCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCgggg                                                                                                                                            <  2:207336/149‑1 (MQ=255)
         ccGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGc                                                                                                                                           <  2:918679/149‑1 (MQ=255)
         ccGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGc                                                                                                                                           <  1:175478/149‑1 (MQ=255)
          cGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGcc                                                                                                                                          >  1:608344/1‑149 (MQ=255)
            gTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCt                                                                                                                                        <  1:643317/149‑1 (MQ=255)
               aTGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGaa                                                                                                                                     <  2:1330333/149‑1 (MQ=255)
               aTGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGaa                                                                                                                                     <  4:697697/149‑1 (MQ=255)
               aTGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGaa                                                                                                                                     <  4:782475/149‑1 (MQ=255)
                tGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAg                                                                                                                                    <  2:1208790/149‑1 (MQ=255)
                  gCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCggg                                                                                                                                             <  3:799456/138‑1 (MQ=255)
                  gCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCggg                                                                                                                                             >  4:799456/1‑138 (MQ=255)
                   cAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGt                                                                                                                                 >  3:941769/1‑149 (MQ=255)
                   cAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGt                                                                                                                                 <  3:1455123/149‑1 (MQ=255)
                   cAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGt                                                                                                                                 <  4:258799/149‑1 (MQ=255)
                    aGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGtt                                                                                                                                >  3:757124/1‑149 (MQ=255)
                    aGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGtt                                                                                                                                <  1:520736/149‑1 (MQ=255)
                     gTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTa                                                                                                                               <  2:1458055/149‑1 (MQ=255)
                        cTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGtt                                                                                                                            >  1:123998/1‑149 (MQ=255)
                         ttCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGttt                                                                                                                           >  2:845595/1‑149 (MQ=255)
                         ttCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGttt                                                                                                                           <  1:1104472/149‑1 (MQ=255)
                          tCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGtttt                                                                                                                          <  4:364212/149‑1 (MQ=255)
                                tACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGtttt                                                                                                                          >  2:1266364/1‑143 (MQ=255)
                                 aCGCCATGGCATTAGACTGACAAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACtgt                                                                                                                   <  3:352347/149‑1 (MQ=255)
                                   gCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGa                                                                                                                 >  2:638851/1‑149 (MQ=255)
                                   gCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGa                                                                                                                 >  3:178297/1‑149 (MQ=255)
                                   gCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGa                                                                                                                 <  2:459568/149‑1 (MQ=255)
                                    ccATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGAc                                                                                                                <  1:2902/149‑1 (MQ=255)
                                      aTGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACtt                                                                                                              >  1:1234706/1‑149 (MQ=255)
                                       tGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACttt                                                                                                             >  4:196260/1‑149 (MQ=255)
                                            ttAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCTTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTa                                                                                                                       <  2:411769/134‑1 (MQ=255)
                                            ttAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTa                                                                                                                       >  1:411769/1‑134 (MQ=255)
                                             tAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATc                                                                                                       <  2:874696/149‑1 (MQ=255)
                                               gACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCt                                                                                                     >  2:547995/1‑149 (MQ=255)
                                                 cTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGcc                                                                                                                                          <  3:889036/110‑1 (MQ=255)
                                                 cTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGcc                                                                                                                                          >  4:889036/1‑110 (MQ=255)
                                                 cTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTc                                                                                                   >  3:1296728/1‑149 (MQ=255)
                                                    aCCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTcaca                                                                                                <  2:1271075/149‑1 (MQ=255)
                                                        gCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGa                                                                                            <  2:470061/149‑1 (MQ=255)
                                                          aaCGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCTCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATc                                                                                          >  3:1200536/1‑149 (MQ=255)
                                                              gTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCaaaa                                                                                      <  1:1368129/149‑1 (MQ=255)
                                                                aaGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACt                                                                                    <  2:1347663/149‑1 (MQ=255)
                                                                  gAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTACTTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTg                                                                                             <  4:1213410/138‑1 (MQ=255)
                                                                  gAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCt                                                                                                                                        >  3:1164259/1‑95 (MQ=255)
                                                                  gAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGa                                                                                  <  2:1282510/149‑1 (MQ=255)
                                                                     cATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATc                                                                               <  2:1234706/149‑1 (MQ=255)
                                                                      aTAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGtt                                                                                                                            >  3:1252618/1‑103 (MQ=255)
                                                                        aGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGt                                                                            >  2:756595/1‑149 (MQ=255)
                                                                        aGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGt                                                                            <  2:733436/149‑1 (MQ=255)
                                                                         gTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTc                                                                           >  2:1073053/1‑149 (MQ=255)
                                                                          taataaTCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACt                                                                                                                     <  1:512060/106‑1 (MQ=255)
                                                                           aataatCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCAc                                                                         >  1:352491/1‑149 (MQ=255)
                                                                            ataatCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCGGTAAAGGGGCCCTgct                                                                                                                                     <  1:162273/88‑3 (MQ=38)
                                                                            ataatCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCGGTAAAGGGGCCCTgct                                                                                                                                     >  2:162273/1‑86 (MQ=37)
                                                                                 cGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCttt                                                                   <  2:2436/149‑1 (MQ=255)
                                                                                      gATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTgccgc                                                              >  4:649900/1‑149 (MQ=255)
                                                                                                cGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTc                                                    <  4:1239846/149‑1 (MQ=255)
                                                                                                 gTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCa                                                   >  1:298838/1‑149 (MQ=255)
                                                                                                   tCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTg                                                                                                                         >  4:2523/1‑77 (MQ=255)
                                                                                                   tCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTg                                                                                                                         <  3:2523/77‑1 (MQ=255)
                                                                                                     atatAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGc                                               >  2:542826/1‑149 (MQ=255)
                                                                                                            tttCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGtt                                                                                                                                <  2:88392/61‑1 (MQ=255)
                                                                                                            tttCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGtt                                                                                                                                >  1:88392/1‑61 (MQ=255)
                                                                                                            tttCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCaataat                                        <  4:889723/149‑1 (MQ=255)
                                                                                                                gcgTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAAt                                    >  1:1289461/1‑149 (MQ=255)
                                                                                                                 cgTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATa                                   >  1:822875/1‑149 (MQ=255)
                                                                                                                  gTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGaa                                                                                 <  3:551743/102‑1 (MQ=255)
                                                                                                                  gTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGaa                                                                                 >  4:551743/1‑102 (MQ=255)
                                                                                                                   tAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGa                                 <  1:1340335/149‑1 (MQ=255)
                                                                                                                    aaTTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGAc                                <  2:608344/149‑1 (MQ=255)
                                                                                                                     aTTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACt                               >  1:740051/1‑149 (MQ=255)
                                                                                                                      ttGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACta                              >  1:333681/1‑149 (MQ=255)
                                                                                                                      ttGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACta                              >  4:322239/1‑149 (MQ=255)
                                                                                                                         aaaTGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACtatta                           >  4:616323/1‑149 (MQ=255)
                                                                                                                            tGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTc                        >  3:523022/1‑149 (MQ=255)
                                                                                                                              aCAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCAcc                                                 >  2:812187/1‑122 (MQ=255)
                                                                                                                              aCAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCAcc                                                 <  1:812187/122‑1 (MQ=255)
                                                                                                                                aaaTTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCaataa                                         >  1:1448144/1‑128 (MQ=255)
                                                                                                                                aaaTTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCaataa                                         <  2:1448144/128‑1 (MQ=255)
                                                                                                                                aaaTTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAgg                    <  3:322239/149‑1 (MQ=255)
                                                                                                                                  aTTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGc                  >  2:986410/1‑149 (MQ=255)
                                                                                                                                    tGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCaa                <  1:986410/149‑1 (MQ=255)
                                                                                                                                     gATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAAt               <  3:1432660/149‑1 (MQ=255)
                                                                                                                                       cttcAGGGCCCCGGTAAAGGGGCCCTGCAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGa             >  3:1178277/4‑149 (MQ=255)
                                                                                                                                        ttcAGGGCCCCGGTAAAGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGAt            >  2:277803/3‑149 (MQ=255)
                                                                                                                                        ttcAGGGCCCCGGTAAAGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGAt            >  1:317948/3‑149 (MQ=255)
                                                                                                                                        ttcAGGGCCCCGGTAAAGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGAt            >  3:1255660/3‑149 (MQ=255)
                                                                                                                                        aGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGAt            >  3:1337807/1‑149 (MQ=255)
                                                                                                                                         gCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGATa           <  4:1311381/149‑1 (MQ=255)
                                                                                                                                           aGGGCCCCGGTAAAGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGa                                 <  1:844021/125‑1 (MQ=255)
                                                                                                                                             ggCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAACCTGAATCCGTCACCGCTTTg                                                                  <  4:1039650/90‑1 (MQ=255)
                                                                                                                                             ggCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTg                                                                  >  3:1039650/1‑90 (MQ=255)
                                                                                                                                                  cTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGATACTCCGTAgg  >  3:1264602/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CTTCCCTGTCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGATACTCCGTAGG  >  NC_000913/4537492‑4537786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: