Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,537,644 C→A 5.3% intergenic (‑50/+15) nanS ← / ← nanM 9‑O‑acetyl N‑acetylneuraminic acid esterase/N‑acetylneuraminic acid mutarotase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,537,6440CA 5.3% 460.7 / 10.8 133intergenic (‑50/+15)nanS/nanM9‑O‑acetyl N‑acetylneuraminic acid esterase/N‑acetylneuraminic acid mutarotase
Reads supporting (aligned to +/- strand):  ref base C (57/69);  new base A (5/2);  total (62/71)
Fisher's exact test for biased strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01

Only 100 of 133 total aligned reads displayed.

CTGTCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGATACTCCGTAGGCCC  >  NC_000913/4537497‑4537789
                                                                                                                                                   |                                                                                                                                                 
cTGTCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCg                                                                                                                                                  >  3:782475/1‑149 (MQ=255)
 tGTCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCgg                                                                                                                                                 >  3:948453/1‑149 (MQ=255)
  gTCCGGTAATGGCAGTCCTTCGTCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCggg                                                                                                                                                <  3:894904/149‑1 (MQ=255)
   tCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCgggg                                                                                                                                               <  2:207336/149‑1 (MQ=255)
   tCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCgggg                                                                                                                                               <  4:171450/149‑1 (MQ=255)
    ccGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGc                                                                                                                                              <  2:918679/149‑1 (MQ=255)
    ccGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGc                                                                                                                                              <  1:175478/149‑1 (MQ=255)
       gTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCt                                                                                                                                           <  1:643317/149‑1 (MQ=255)
          aTGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGaa                                                                                                                                        <  2:1330333/149‑1 (MQ=255)
          aTGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGaa                                                                                                                                        <  4:697697/149‑1 (MQ=255)
            ggCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAATAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTGCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGc                                                                                                                                      <  4:286314/149‑1 (MQ=255)
             gCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCggg                                                                                                                                                >  4:799456/1‑138 (MQ=255)
              cAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGt                                                                                                                                    >  3:941769/1‑149 (MQ=255)
              cAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGt                                                                                                                                    <  4:258799/149‑1 (MQ=255)
               aGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGtt                                                                                                                                   >  3:757124/1‑149 (MQ=255)
               aGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGtt                                                                                                                                   <  1:520736/149‑1 (MQ=255)
                gTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTa                                                                                                                                  <  2:1449942/149‑1 (MQ=255)
                gTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTa                                                                                                                                  <  2:1458055/149‑1 (MQ=255)
                   cTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGtt                                                                                                                               >  1:123998/1‑149 (MQ=255)
                    ttCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGttt                                                                                                                              >  2:845595/1‑149 (MQ=255)
                    ttCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGttt                                                                                                                              <  1:1104472/149‑1 (MQ=255)
                     tCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGtttt                                                                                                                             <  4:364212/149‑1 (MQ=255)
                          aTACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACt                                                                                                                        <  2:123998/149‑1 (MQ=255)
                           tACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGtttt                                                                                                                             >  2:1266364/1‑143 (MQ=255)
                           tACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGtttt                                                                                                                             <  1:1266364/143‑1 (MQ=255)
                            aCGCCATGGCATTAGACTGACAAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACtgt                                                                                                                      <  3:352347/149‑1 (MQ=255)
                              gCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGa                                                                                                                    <  2:459568/149‑1 (MQ=255)
                              gCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGa                                                                                                                    >  2:638851/1‑149 (MQ=255)
                              gCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGa                                                                                                                    >  3:178297/1‑149 (MQ=255)
                               ccATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGAc                                                                                                                   <  1:2902/149‑1 (MQ=255)
                                 aTGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACtt                                                                                                                 >  1:1234706/1‑149 (MQ=255)
                                  tGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGGCCCTTTACCGGGGCGCTGAAGCGTTAGTTTTGTACTGTGACttt                                                                                                                <  1:581836/149‑1 (MQ=255)
                                   ggCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGtttt                                                                                                                             <  3:442267/135‑1 (MQ=255)
                                   ggCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGtttt                                                                                                                             >  4:442267/1‑135 (MQ=255)
                                      aTTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTttatta                                                                                                            >  2:168929/1‑149 (MQ=255)
                                       ttAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCTTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTa                                                                                                                          <  2:411769/134‑1 (MQ=255)
                                       ttAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTa                                                                                                                          >  1:411769/1‑134 (MQ=255)
                                        tAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATc                                                                                                          <  3:202357/149‑1 (MQ=255)
                                        tAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATc                                                                                                          <  2:874696/149‑1 (MQ=255)
                                          gACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCt                                                                                                        >  2:547995/1‑149 (MQ=255)
                                            cTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGcc                                                                                                                                             <  3:889036/110‑1 (MQ=255)
                                            cTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGcc                                                                                                                                             >  4:889036/1‑110 (MQ=255)
                                                     aaCGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCTCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATc                                                                                             >  3:1200536/1‑149 (MQ=255)
                                                     aaCGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATc                                                                                             <  4:692422/149‑1 (MQ=255)
                                                         gTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCaaaa                                                                                         <  1:1368129/149‑1 (MQ=255)
                                                             gAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTACTTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTg                                                                                                <  4:1213410/138‑1 (MQ=255)
                                                             gAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTg                                                                                                >  3:1213410/1‑138 (MQ=255)
                                                             gAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGa                                                                                     <  2:1282510/149‑1 (MQ=255)
                                                                cATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATc                                                                                  <  2:1234706/149‑1 (MQ=255)
                                                                   aGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGt                                                                               <  2:733436/149‑1 (MQ=255)
                                                                   aGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGt                                                                               >  2:756595/1‑149 (MQ=255)
                                                                    gTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTc                                                                              >  2:1073053/1‑149 (MQ=255)
                                                                     taataaTCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACt                                                                                                                        <  1:512060/106‑1 (MQ=255)
                                                                     taataaTCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACt                                                                                                                        >  2:512060/1‑106 (MQ=255)
                                                                      aataatCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCAc                                                                            >  1:352491/1‑149 (MQ=255)
                                                                      aataatCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCAc                                                                            >  3:862521/1‑149 (MQ=255)
                                                                      aataatCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCAc                                                                            <  2:1153085/149‑1 (MQ=255)
                                                                       ataatCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCGGTAAAGGGGCCCTgct                                                                                                                                        <  1:162273/88‑3 (MQ=38)
                                                                            cGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCttt                                                                      <  2:2436/149‑1 (MQ=255)
                                                                              ggCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTgc                                                                    >  3:1134557/1‑149 (MQ=255)
                                                                                 gATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTgccgc                                                                 >  4:649900/1‑149 (MQ=255)
                                                                                  atatTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTgccgcc                                                                <  1:845595/149‑1 (MQ=255)
                                                                                           cGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTc                                                       <  4:1239846/149‑1 (MQ=255)
                                                                                            gTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCa                                                      >  1:298838/1‑149 (MQ=255)
                                                                                              tCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTg                                                                                                                            <  3:2523/77‑1 (MQ=255)
                                                                                              tCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTg                                                                                                                            >  4:2523/1‑77 (MQ=255)
                                                                                                atatAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGc                                                  >  2:542826/1‑149 (MQ=255)
                                                                                                       tttCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGtt                                                                                                                                   <  2:88392/61‑1 (MQ=255)
                                                                                                       tttCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCaataat                                           <  4:889723/149‑1 (MQ=255)
                                                                                                          cgcgTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCaa                                        <  4:178297/149‑1 (MQ=255)
                                                                                                            cgTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATa                                      >  1:822875/1‑149 (MQ=255)
                                                                                                             gTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGaa                                                                                    >  4:551743/1‑102 (MQ=255)
                                                                                                              tAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGa                                    <  1:1340335/149‑1 (MQ=255)
                                                                                                              tAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGa                                    <  1:1354838/149‑1 (MQ=255)
                                                                                                               aaTTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGAc                                   <  2:608344/149‑1 (MQ=255)
                                                                                                                aTTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACt                                  >  1:740051/1‑149 (MQ=255)
                                                                                                                 ttGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACta                                 >  1:333681/1‑149 (MQ=255)
                                                                                                                     aaTGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACtattat                             >  3:126833/1‑149 (MQ=255)
                                                                                                                      aTGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTattatt                            >  3:1430894/1‑149 (MQ=255)
                                                                                                                       tGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTc                           >  3:523022/1‑149 (MQ=255)
                                                                                                                         aCAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCAcc                                                    >  2:812187/1‑122 (MQ=255)
                                                                                                                           aaaTTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCaataa                                            >  1:1448144/1‑128 (MQ=255)
                                                                                                                           aaaTTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCaataa                                            <  2:1448144/128‑1 (MQ=255)
                                                                                                                           aaaTTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAgg                       <  2:1289461/149‑1 (MQ=255)
                                                                                                                             aTTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGc                     >  2:986410/1‑149 (MQ=255)
                                                                                                                              ttGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCa                    >  1:789103/1‑149 (MQ=255)
                                                                                                                              ttGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCa                    >  2:300085/1‑149 (MQ=255)
                                                                                                                               tGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCaa                   <  1:986410/149‑1 (MQ=255)
                                                                                                                                gATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAAt                  <  3:1432660/149‑1 (MQ=255)
                                                                                                                                  tAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGa                <  3:649900/149‑1 (MQ=255)
                                                                                                                                  cttcAGGGCCCCGGTAAAGGGGCCCTGCAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGa                >  3:1178277/4‑149 (MQ=255)
                                                                                                                                   ttcAGGGCCCCGGTAAAGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGAt               >  3:1255660/3‑149 (MQ=255)
                                                                                                                                   ttcAGGGCCCCGGTAAAGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGAt               >  1:317948/3‑149 (MQ=255)
                                                                                                                                   aGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGAt               >  3:1337807/1‑149 (MQ=255)
                                                                                                                                    gCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGATa              <  4:1311381/149‑1 (MQ=255)
                                                                                                                                      aGGGCCCCGGTAAAGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGa                                    <  1:844021/125‑1 (MQ=255)
                                                                                                                                        ggCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAACCTGAATCCGTCACCGCTTTg                                                                     <  4:1039650/90‑1 (MQ=255)
                                                                                                                                        ggCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTg                                                                     >  3:1039650/1‑90 (MQ=255)
                                                                                                                                             cTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGATACTCCGTAgg     >  3:1264602/1‑149 (MQ=255)
                                                                                                                                                tACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGATACTCCGTAGGccc  <  4:1207806/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CTGTCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGATACTCCGTAGGCCC  >  NC_000913/4537497‑4537789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: