Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,608,604 C→G 5.3% I140M (ATC→ATG rimI → ribosomal‑protein‑S18‑alanine N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,608,6040CG 5.3% 672.6 / 21.3 189I140M (ATC→ATGrimIribosomal‑protein‑S18‑alanine N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (79/99);  new base G (5/5);  total (84/105)
Fisher's exact test for biased strand distribution p-value = 7.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

Only 100 of 209 total aligned reads displayed.

AACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTACCGC  >  NC_000913/4608456‑4608752
                                                                                                                                                    |                                                                                                                                                    
 aCTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCcatca                                                                                                                                                     >  1:1082484/1‑149 (MQ=255)
    ggAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCcatcatca                                                                                                                                                  <  4:565180/149‑1 (MQ=255)
    ggAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCcatcatca                                                                                                                                                  <  2:464349/149‑1 (MQ=255)
           cgcgGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATGATGATGGCGtc                                                                                                                                           <  2:1354724/149‑2 (MQ=255)
            gcggcgTGGCGACACTATGGCTGTAAGTCCGTGCATCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAACACGCCATCATCATGGCGtta                                                                                                                                          >  2:1028916/1‑148 (MQ=255)
                cgTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCaa                                                                                                                                      <  4:1372930/149‑1 (MQ=255)
                     cGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGt                                                                                                                                 <  4:1104990/149‑1 (MQ=255)
                      gACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTa                                                                                                                                <  1:1141622/149‑1 (MQ=255)
                      gACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTa                                                                                                                                >  2:1239058/1‑149 (MQ=255)
                         acTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGt                                                                                                                             <  3:524735/149‑1 (MQ=255)
                           tATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTaa                                                                                                                           >  1:912849/1‑149 (MQ=255)
                             tGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATa                                                                                                                         >  4:906665/1‑149 (MQ=255)
                               gCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACa                                                                                                                       >  3:552559/1‑149 (MQ=255)
                                cTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACaa                                                                                                                      <  1:592005/149‑1 (MQ=255)
                                 tGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAg                                                                                                                     >  2:597685/1‑149 (MQ=255)
                                 tGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAg                                                                                                                     <  4:1110180/149‑1 (MQ=255)
                                   gAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGt                                                                                                                   >  1:967074/1‑149 (MQ=255)
                                    aaGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTg                                                                                                                  <  2:148612/149‑1 (MQ=255)
                                    aaGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTg                                                                                                                  <  4:484665/149‑1 (MQ=255)
                                    aaGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTg                                                                                                                  >  3:609901/1‑149 (MQ=255)
                                       tCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGaa                                                                                                               >  1:1271317/1‑149 (MQ=255)
                                       tCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGaa                                                                                                               >  4:255524/1‑149 (MQ=255)
                                          gTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGaataa                                                                                                            >  1:680321/1‑149 (MQ=255)
                                          gTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGaataa                                                                                                            >  4:829338/1‑149 (MQ=255)
                                          gTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGaataa                                                                                                            >  2:344764/1‑149 (MQ=255)
                                          gTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGaataa                                                                                                            <  2:1043608/149‑1 (MQ=255)
                                              ttCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGaa                                                                                                               >  3:531524/1‑142 (MQ=255)
                                              ttCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGaa                                                                                                        <  3:821619/149‑1 (MQ=255)
                                              ttCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGaa                                                                                                        <  3:1293781/149‑1 (MQ=255)
                                              ttCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGaa                                                                                                        >  1:644975/1‑149 (MQ=255)
                                              ttCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGaa                                                                                                        <  1:32971/149‑1 (MQ=255)
                                                cAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCcatcat                                                                                                                                                    >  1:1041983/1‑103 (MQ=255)
                                                 aaaCGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTg                                                                                                     <  2:138470/149‑1 (MQ=255)
                                                 aaaCGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTg                                                                                                     >  3:87902/1‑149 (MQ=255)
                                                 aaaCGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTg                                                                                                     <  2:662204/149‑1 (MQ=255)
                                                   aCGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTggg                                                                                                   >  2:785374/1‑149 (MQ=255)
                                                       tGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTg                                                                                               <  2:1122199/149‑1 (MQ=255)
                                                           gccATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTGCTGCCGCACCACGGACGGGCGCGAAGACGCCATCGTCTTGGCGTTGGCTGTCAGTATGTAATACAAGGTGGAATAATGAAGTGGGAGTGGAtt                                                                                           <  4:1400007/149‑1 (MQ=255)
                                                           gccATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGAtt                                                                                           >  1:350008/1‑149 (MQ=255)
                                                           gccATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGAtt                                                                                           >  2:911455/1‑149 (MQ=255)
                                                           gccATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACAGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGAtt                                                                                           >  1:237990/1‑149 (MQ=255)
                                                            ccATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGAttt                                                                                          >  4:1329184/1‑149 (MQ=255)
                                                             cATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGAc                                                                                                 <  3:1220285/141‑1 (MQ=255)
                                                             cATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGAc                                                                                                 >  4:1220285/1‑141 (MQ=255)
                                                                 gCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCttt                                                                                     <  1:426209/149‑1 (MQ=255)
                                                                 gCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCttt                                                                                     <  1:458874/149‑1 (MQ=255)
                                                                 gCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCttt                                                                                     <  3:757021/149‑1 (MQ=255)
                                                                  cccTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTg                                                                                    >  3:1032143/1‑149 (MQ=255)
                                                                        aCGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCg                                                                              <  4:1052714/149‑1 (MQ=255)
                                                                          gAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCtt                                                                                      >  4:425946/1‑139 (MQ=255)
                                                                          gAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGAt                                                                            >  4:194927/1‑149 (MQ=255)
                                                                          gAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGAt                                                                            >  3:365460/1‑149 (MQ=255)
                                                                            aaGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGa                                                                          >  3:627995/1‑149 (MQ=255)
                                                                            aaGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACAACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGa                                                                          >  4:842794/1‑149 (MQ=255)
                                                                               tttAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAAc                                                                       >  3:154774/1‑149 (MQ=255)
                                                                               tttAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAAc                                                                       <  3:794114/149‑1 (MQ=255)
                                                                                    gCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATg                                                                                                                                                <  4:538723/71‑1 (MQ=255)
                                                                                    gCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGt                                                                  <  1:1326282/149‑1 (MQ=255)
                                                                                    gCTTTAACGAGACGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGa                                                                                             >  1:775526/1‑122 (MQ=255)
                                                                                     cTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGtt                                                                 <  2:1431064/149‑1 (MQ=255)
                                                                                     cTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGtt                                                                 >  1:1193181/1‑149 (MQ=255)
                                                                                      tttAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGttt                                                                >  2:57711/1‑149 (MQ=255)
                                                                                         aaCGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTCCTTTGATGCCGATGAAACGCTGTTTAcc                                                             <  3:1366132/149‑1 (MQ=255)
                                                                                              ggCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGa                                                        <  4:1032143/149‑1 (MQ=255)
                                                                                              ggCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGa                                                        <  3:424713/149‑1 (MQ=255)
                                                                                              ggCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGa                                                        <  2:615737/149‑1 (MQ=255)
                                                                                               gCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGAc                                                       <  1:1277476/149‑1 (MQ=255)
                                                                                                 gacgaTTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTc                                                     <  4:1415245/149‑1 (MQ=255)
                                                                                                 gacgaTTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTc                                                     >  1:1380494/1‑149 (MQ=255)
                                                                                                 gacgaTTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTc                                                     <  2:773783/149‑1 (MQ=255)
                                                                                                 gacgaTTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTc                                                     >  4:944524/1‑149 (MQ=255)
                                                                                                    gaTTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCAtt                                                  >  4:717903/1‑149 (MQ=255)
                                                                                                         gccgcAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCg                                             <  3:1329184/149‑1 (MQ=255)
                                                                                                         gccgcAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCg                                             >  3:310061/1‑149 (MQ=255)
                                                                                                                ttACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCa                                      >  2:727854/1‑149 (MQ=255)
                                                                                                                ttACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCa                                      >  1:110388/1‑149 (MQ=255)
                                                                                                                  actacCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGc                                    >  1:364158/1‑149 (MQ=255)
                                                                                                                    tacCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTc                                                                                        >  2:274731/1‑95 (MQ=255)
                                                                                                                    tacCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTc                                                                                        <  1:274731/95‑1 (MQ=255)
                                                                                                                    tacCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCgg                                  <  3:745143/149‑1 (MQ=255)
                                                                                                                      ccccACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGAt                                >  4:762954/1‑149 (MQ=255)
                                                                                                                       cccACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATg                               >  1:245110/1‑149 (MQ=255)
                                                                                                                       cccACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATg                               <  3:906665/149‑1 (MQ=255)
                                                                                                                           ccacGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTc                           <  4:144078/149‑1 (MQ=255)
                                                                                                                            cacGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCt                          <  3:717903/149‑1 (MQ=255)
                                                                                                                             acggacggTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCtt                         <  1:601854/149‑1 (MQ=255)
                                                                                                                                gacggTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGAt                      <  2:1149497/149‑1 (MQ=255)
                                                                                                                                gacggTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGAt                      <  4:627995/149‑1 (MQ=255)
                                                                                                                                       gcgAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCg                                   >  3:110558/1‑129 (MQ=255)
                                                                                                                                         gAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTc             >  1:1446642/1‑149 (MQ=255)
                                                                                                                                         gAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTc             <  2:110388/149‑1 (MQ=255)
                                                                                                                                           aGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCAc           <  4:310061/149‑1 (MQ=255)
                                                                                                                                            gACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCAcc          >  4:1282733/1‑149 (MQ=255)
                                                                                                                                            aaCGCCATGATGATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCAcc          >  3:1186337/2‑149 (MQ=255)
                                                                                                                                            aaCGCCATGATGATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCAcc          >  2:913676/2‑149 (MQ=255)
                                                                                                                                            aaCGCCATGATGATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCAcc          >  4:1177214/2‑149 (MQ=255)
                                                                                                                                                 catcatcatGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTAc     >  4:536848/1‑149 (MQ=255)
                                                                                                                                                 catcatcatGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTAc     >  3:262735/1‑149 (MQ=255)
                                                                                                                                                 catcatcatGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTAc     >  3:991298/1‑149 (MQ=255)
                                                                                                                                                    catcatGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTACCGc  <  4:659196/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AACTGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTACCGC  >  NC_000913/4608456‑4608752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: