Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,608,607 C→G 5.3% I141M (ATC→ATG rimI → ribosomal‑protein‑S18‑alanine N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,608,6070CG 5.3% 676.5 / 19.6 189I141M (ATC→ATGrimIribosomal‑protein‑S18‑alanine N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (79/100);  new base G (5/5);  total (84/105)
Fisher's exact test for biased strand distribution p-value = 7.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 213 total aligned reads displayed.

TGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTACCGCTGA  >  NC_000913/4608459‑4608755
                                                                                                                                                    |                                                                                                                                                    
 ggAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCcatcatca                                                                                                                                                     <  4:565180/149‑1 (MQ=255)
 ggAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCcatcatca                                                                                                                                                     >  1:243264/1‑149 (MQ=255)
  gAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCcatcatcat                                                                                                                                                    >  4:1333157/1‑149 (MQ=255)
   aaaaaCGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATg                                                                                                                                                   >  4:223681/1‑149 (MQ=255)
   aaaaaCGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATg                                                                                                                                                   <  2:1472969/149‑1 (MQ=255)
        cgcgGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATGATGATGGCGtc                                                                                                                                              <  2:1354724/149‑2 (MQ=255)
                   gACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGc                                                                                                                                            >  1:626381/1‑140 (MQ=255)
                   gACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTa                                                                                                                                   <  1:1141622/149‑1 (MQ=255)
                   gACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTa                                                                                                                                   >  2:1239058/1‑149 (MQ=255)
                    acacTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTAt                                                                                                                                  <  4:137608/149‑1 (MQ=255)
                    acacTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTAt                                                                                                                                  >  3:143153/1‑149 (MQ=255)
                      acTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGt                                                                                                                                >  4:745143/1‑149 (MQ=255)
                        tATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCcatcatca                                                                                                                                                     <  2:367222/126‑1 (MQ=255)
                        tATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTaa                                                                                                                              >  1:912849/1‑149 (MQ=255)
                          tGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATa                                                                                                                            >  4:906665/1‑149 (MQ=255)
                            gCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACa                                                                                                                          >  3:552559/1‑149 (MQ=255)
                              tGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACCACCCCCCACGGACGGTCGCGGAGACGCCATCATCCTGGCGTTGCCAATCAGTATGTAAAACCAgg                                                                                                                       >  4:823799/1‑149 (MQ=255)
                                 aaGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTg                                                                                                                     <  4:484665/149‑1 (MQ=255)
                                 aaGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTg                                                                                                                     <  1:702478/149‑1 (MQ=255)
                                    tCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGaa                                                                                                                  >  1:1271317/1‑149 (MQ=255)
                                       gTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGaataa                                                                                                               <  2:1043608/149‑1 (MQ=255)
                                          cTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGa                                                                                                            >  3:659196/1‑149 (MQ=255)
                                           ttCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGaa                                                                                                                  <  4:531524/142‑1 (MQ=255)
                                           ttCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGaa                                                                                                           <  3:821619/149‑1 (MQ=255)
                                           ttCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGaa                                                                                                           <  1:32971/149‑1 (MQ=255)
                                             cAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGt                                                                                                         <  3:756898/149‑1 (MQ=255)
                                              aaaCGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTg                                                                                                        <  2:662204/149‑1 (MQ=255)
                                              aaaCGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTg                                                                                                        >  3:87902/1‑149 (MQ=255)
                                               aaCGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATGATGATGGCGtct                                                                                                                                             >  1:836508/1‑109 (MQ=255)
                                                aCGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTggg                                                                                                      >  2:785374/1‑149 (MQ=255)
                                                    tGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTg                                                                                                  <  2:1122199/149‑1 (MQ=255)
                                                      ccgccATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGa                                                                                                <  3:1333157/149‑1 (MQ=255)
                                                        gccATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGAtt                                                                                              <  2:868628/149‑1 (MQ=255)
                                                        gccATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGAtt                                                                                              >  1:350008/1‑149 (MQ=255)
                                                            ttGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCt                                                                                          <  2:47888/149‑1 (MQ=255)
                                                             tGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCtt                                                                                         <  2:912849/149‑1 (MQ=255)
                                                             tGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCtt                                                                                         <  2:58995/149‑1 (MQ=255)
                                                             tGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCtt                                                                                         <  4:1048449/149‑1 (MQ=255)
                                                              gCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCttt                                                                                        <  4:1151511/149‑1 (MQ=255)
                                                              gCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCttt                                                                                        <  1:458874/149‑1 (MQ=255)
                                                              gCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCttt                                                                                        <  1:597685/149‑1 (MQ=255)
                                                                 cTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGAt                                                                                     <  3:11192/149‑1 (MQ=255)
                                                                    tACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGcc                                                                                  >  4:1321882/1‑149 (MQ=255)
                                                                       gAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCtt                                                                                         >  4:425946/1‑139 (MQ=255)
                                                                       gAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCtt                                                                                         <  3:425946/139‑1 (MQ=255)
                                                                            tttAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAAc                                                                          <  3:794114/149‑1 (MQ=255)
                                                                                 gCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATg                                                                                                                                                   <  4:538723/71‑1 (MQ=255)
                                                                                 gCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGt                                                                     <  1:1326282/149‑1 (MQ=255)
                                                                                  cTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGtt                                                                    <  2:1431064/149‑1 (MQ=255)
                                                                                  cTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGtt                                                                    <  2:1011772/149‑1 (MQ=255)
                                                                                  cTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGtt                                                                    >  1:1193181/1‑149 (MQ=255)
                                                                                   tttAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGttt                                                                   >  3:415647/1‑149 (MQ=255)
                                                                                      aaCGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTAcc                                                                <  3:308162/149‑1 (MQ=255)
                                                                                       aCGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCt                                                               >  3:728302/1‑149 (MQ=255)
                                                                                           ggCGACGATTCGCCGCAATTACTTCCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGa                                                           <  4:127874/149‑1 (MQ=255)
                                                                                           ggCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGa                                                           <  1:1078744/149‑1 (MQ=255)
                                                                                           ggCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGa                                                           <  2:615737/149‑1 (MQ=255)
                                                                                           ggCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGa                                                           <  1:679522/149‑1 (MQ=255)
                                                                                            gCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGAc                                                          <  1:1277476/149‑1 (MQ=255)
                                                                                              gacgaTTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTc                                                        <  2:773783/149‑1 (MQ=255)
                                                                                              gacgaTTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTc                                                        >  1:1380494/1‑149 (MQ=255)
                                                                                              gacgaTTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTc                                                        >  4:944524/1‑149 (MQ=255)
                                                                                              gacgaTTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTc                                                        >  4:188243/1‑149 (MQ=255)
                                                                                               acgaTTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCa                                                       >  4:832012/1‑149 (MQ=255)
                                                                                                cgaTTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCAt                                                      <  1:236274/149‑1 (MQ=255)
                                                                                                         gcAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGcc                                             >  4:1012881/1‑149 (MQ=255)
                                                                                                           aaTTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTg                                           >  3:357924/1‑149 (MQ=255)
                                                                                                             ttACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCa                                         >  1:110388/1‑149 (MQ=255)
                                                                                                             ttACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCa                                         >  2:727854/1‑149 (MQ=255)
                                                                                                               actacCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGc                                       >  1:364158/1‑149 (MQ=255)
                                                                                                                 tacCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTc                                                                                           <  1:274731/95‑1 (MQ=255)
                                                                                                                 tacCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTc                                                                                           >  2:274731/1‑95 (MQ=255)
                                                                                                                     ccaccaCGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGa                                    <  3:611216/146‑1 (MQ=255)
                                                                                                                     ccaccaCGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGt                                 <  3:762954/149‑1 (MQ=255)
                                                                                                                      caccacGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGtt                                <  4:143153/149‑1 (MQ=255)
                                                                                                                          acggacggTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACCAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCtt                            <  3:1259099/149‑1 (MQ=255)
                                                                                                                           cggacggTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTg                           <  3:1321882/149‑1 (MQ=255)
                                                                                                                             gacggTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGAt                         <  4:627995/149‑1 (MQ=255)
                                                                                                                             gacggTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGAt                         <  2:1149497/149‑1 (MQ=255)
                                                                                                                                ggTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTAc                      <  3:944524/149‑1 (MQ=255)
                                                                                                                                 gTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACa                     <  2:915100/149‑1 (MQ=255)
                                                                                                                                  tCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAg                    >  4:689955/1‑149 (MQ=255)
                                                                                                                                  tCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAg                    >  3:531354/1‑149 (MQ=255)
                                                                                                                                    gcgAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCg                                      <  4:110558/129‑1 (MQ=255)
                                                                                                                                      gAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTc                <  2:110388/149‑1 (MQ=255)
                                                                                                                                        aGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCAc              <  4:310061/149‑1 (MQ=255)
                                                                                                                                         gACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCAcc             >  1:245589/1‑149 (MQ=255)
                                                                                                                                            gCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCgg                                               >  3:1193724/1‑112 (MQ=255)
                                                                                                                                            gCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCgg                                               <  4:1193724/112‑1 (MQ=255)
                                                                                                                                              catcatcatGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTAc        >  4:536848/1‑149 (MQ=255)
                                                                                                                                              catcatcatGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTAc        >  3:262735/1‑149 (MQ=255)
                                                                                                                                              caccatcgtGCCGCTGCCAATCAGAATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTAc        >  4:1405553/4‑149 (MQ=255)
                                                                                                                                                tcatcatGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTACCg      >  4:1059094/1‑149 (MQ=255)
                                                                                                                                                  atcatGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTg                                                                                                        <  3:535868/49‑1 (MQ=255)
                                                                                                                                                  atcatGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTACCGCt    >  2:208778/1‑149 (MQ=255)
                                                                                                                                                  atcatGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTACCGCt    >  2:1391542/1‑149 (MQ=255)
                                                                                                                                                  atcatGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTACCGCt    <  1:57711/149‑1 (MQ=255)
                                                                                                                                                  atcatGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTACCGCt    >  4:875837/1‑149 (MQ=255)
                                                                                                                                                   tcatGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTACCGCTg   >  1:413457/1‑149 (MQ=255)
                                                                                                                                                    catGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTACCGCTGa  <  3:1059094/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTACCGCTGA  >  NC_000913/4608459‑4608755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: