Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 832,252 T→A 6.7% intergenic (+16/+216) rhlE → / ← ybiA ATP‑dependent RNA helicase/DUF1768 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913832,2520TA 6.7% 410.5 / 14.2 116intergenic (+16/+216)rhlE/ybiAATP‑dependent RNA helicase/DUF1768 family protein
Reads supporting (aligned to +/- strand):  ref base T (46/62);  new base A (4/4);  total (50/66)
Fisher's exact test for biased strand distribution p-value = 7.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.06e-01

Only 100 of 116 total aligned reads displayed.

GTGGGGAAGGTGGCGCAAAATCTGCAAGCGCGAAACCTGCAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAG  >  NC_000913/832106‑832399
                                                                                                                                                  |                                                                                                                                                   
gTGGGGAAGGTGGCGCAAAATCTGCAAGCGCGAAACCTGCAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTAt                                                                                                                                                   <  1:715404/149‑1 (MQ=255)
   gggAAGGTGGCGCAAAATCTGCAAGCGCGAAACCTGCAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGcc                                                                                                                                                >  4:493370/1‑149 (MQ=255)
    ggAAGGTGGCGCAAAATCTGCAAGCGCGAAACCTGCAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGccc                                                                                                                                               <  4:1372719/149‑1 (MQ=255)
        ggTGGCGCAAAATCTGCAAGCGCGAAACCTGCAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCATAGCCCGGCa                                                                                                                                           <  4:1395079/149‑1 (MQ=255)
        ggTGGCGCAAAATCTGCAAGCGCGAAACCTGCAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCATAGCCCGGCa                                                                                                                                           <  1:14282/149‑1 (MQ=255)
         gTGGCGCAAAATCTGCAAGCGCGAAACCTGCAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCATAGCCCGGCAt                                                                                                                                          >  4:92831/1‑149 (MQ=255)
           ggCGCAAAATCTGCAAGCGCGAAACCTGCAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCa                                                                                                                                        <  1:1160593/149‑1 (MQ=255)
           ggCGCAAAATCTGCAAGCGCGAAACCTGCAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCa                                                                                                                                        <  2:1001497/149‑1 (MQ=255)
            gcgcAAAATCTGCAAGCGCGAAACCTGCAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAg                                                                                                                                       <  2:1319159/149‑1 (MQ=255)
             cgcAAAATCTGCAAGCGCGAAACCTGCAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCATAGCCCGGCAtaaaa                                                                                                                                      <  3:92831/149‑5 (MQ=255)
              gcAAAATCTGCAAGCGCGAAACCTGCAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGc                                                                                                                                     >  1:1161047/1‑149 (MQ=255)
                aaaaTCTGCAAGCGCGAAACCTGCAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCtg                                                                                                                                   <  1:197860/149‑1 (MQ=255)
                 aaaTCTGCAAGCGCGAAACCTGCAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCtga                                                                                                                                  >  4:1209467/1‑149 (MQ=255)
                 aaaTCTGCAAGCGCGAAACCTGCAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCtga                                                                                                                                  <  2:896455/149‑1 (MQ=255)
                  aaTCTGCAAGCGCGAAACCTGCAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCtgat                                                                                                                                 <  1:1255544/149‑1 (MQ=255)
                     cTGCAAGCGCGAAACCTGCAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGaa                                                                                                                              <  2:1352295/149‑1 (MQ=255)
                     cTGCAAGCGCGAAACCTGCAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGaa                                                                                                                              <  4:945203/149‑1 (MQ=255)
                      tGCAAGCGCGAAACCTGCAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAAc                                                                                                                             <  3:134115/149‑1 (MQ=255)
                      tGCAAGCGCGAAACCTGCAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAAc                                                                                                                             <  1:856846/149‑1 (MQ=255)
                          aGCGCGAAACCTGCAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAAc                                                                                                                         >  3:993207/1‑149 (MQ=255)
                               gAAACCTGCAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCaaa                                                                                                                    >  2:1076585/1‑149 (MQ=255)
                               gAAACCTGCAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCaaa                                                                                                                    >  1:1453273/1‑149 (MQ=255)
                                    cTGCAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGc                                                                                                               <  3:758285/149‑1 (MQ=255)
                                      gCAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCt                                                                                                             >  3:1004656/1‑149 (MQ=255)
                                      gCAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCt                                                                                                             <  1:306149/149‑1 (MQ=255)
                                       cAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCtga                                                                                                                                  <  1:608958/127‑1 (MQ=255)
                                       cAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCtga                                                                                                                                  >  2:608958/1‑127 (MQ=255)
                                        aGAAAAACCGTCTCGCTGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGa                                                                                                           <  3:1244198/149‑1 (MQ=255)
                                         gAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGAt                                                                                                          >  4:815517/1‑149 (MQ=255)
                                         gAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATAAACAAACGCAAAACTGCCTGAt                                                                                                          >  3:151763/1‑149 (MQ=255)
                                          aaaaaCCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATg                                                                                                         >  2:927645/1‑149 (MQ=255)
                                             aaCCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATgcgc                                                                                                      >  1:902440/1‑149 (MQ=255)
                                              aCCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGc                                                                                                               >  2:718081/1‑139 (MQ=255)
                                              aCCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGc                                                                                                               <  1:718081/139‑1 (MQ=255)
                                                cGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTAc                                                                                                   >  1:199387/1‑149 (MQ=255)
                                                  tctcGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGc                                                                                                 <  4:537431/149‑1 (MQ=255)
                                                  tctcGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGc                                                                                                 <  4:302625/149‑1 (MQ=255)
                                                      gccgccTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTAt                                                                                             >  4:657227/1‑149 (MQ=255)
                                                        cgccTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCa                                                                                           <  3:815517/149‑1 (MQ=255)
                                                        cgccTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCa                                                                                           <  2:1024975/149‑1 (MQ=255)
                                                         gccTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAg                                                                                          >  1:947747/1‑149 (MQ=255)
                                                         gccTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCAGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAg                                                                                          <  2:1331516/149‑1 (MQ=255)
                                                            tCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGcc                                                                                       >  1:1096289/1‑149 (MQ=255)
                                                             cGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCt                                                                                      <  4:993207/149‑1 (MQ=255)
                                                                 gATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGc                                                                                                               >  4:85321/1‑120 (MQ=255)
                                                                    gCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGaa                                                                               <  3:1023650/149‑1 (MQ=255)
                                                                     ccAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAAc                                                                              <  3:203677/149‑1 (MQ=255)
                                                                      cAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTAt                                                                                                                                                   <  2:972523/79‑1 (MQ=255)
                                                                       aaaCCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACaaa                                                                                                                          <  2:1148427/103‑1 (MQ=255)
                                                                       aaaCCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACaaa                                                                                                                          >  1:1148427/1‑103 (MQ=255)
                                                                       aaaCCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAActc                                                                            >  1:84739/1‑149 (MQ=255)
                                                                                ggCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAatata                                                                   <  4:145068/149‑1 (MQ=255)
                                                                                ggCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAatata                                                                   >  4:343650/1‑149 (MQ=255)
                                                                                 gCGAACAACAACGTCGCCGCCGTCCGCGTAAGCCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAatatat                                                                  >  4:892878/1‑149 (MQ=255)
                                                                                  cGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATAtt                                                                 <  2:1453273/149‑1 (MQ=255)
                                                                                  cGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATAtt                                                                 <  2:1314183/149‑1 (MQ=255)
                                                                                  cGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATAtt                                                                 <  2:1262065/149‑1 (MQ=255)
                                                                                     acaacaacGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGaa                                                              <  2:543888/149‑1 (MQ=255)
                                                                                     acaacaacGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGaa                                                              <  3:474447/149‑1 (MQ=255)
                                                                                       aacaacGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAAtt                                                            >  4:719749/1‑149 (MQ=255)
                                                                                        acaacGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAAttt                                                           <  3:1109391/149‑1 (MQ=255)
                                                                                         caacGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTTCGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTg                                                          <  3:892878/149‑1 (MQ=255)
                                                                                             gTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATg                                                      <  3:9046/149‑1 (MQ=255)
                                                                                                gccgcgGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGTCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCtt                                                   <  4:620072/149‑1 (MQ=255)
                                                                                                  cgccgTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCg                                                                                                                                              >  2:952116/1‑56 (MQ=255)
                                                                                                  cgccgTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCg                                                                                                                                              <  1:952116/56‑1 (MQ=255)
                                                                                                  cgccgTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCtttt                                                 <  1:849535/149‑1 (MQ=255)
                                                                                                     cgTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTa                                              <  4:766582/149‑1 (MQ=255)
                                                                                                      gTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAg                                             <  2:114252/149‑1 (MQ=255)
                                                                                                       tCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGcc                                                                                       <  3:574648/106‑1 (MQ=255)
                                                                                                       tCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGcc                                                                                       >  4:574648/1‑106 (MQ=255)
                                                                                                       tCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAgg                                            >  3:1386335/1‑149 (MQ=255)
                                                                                                         cgcgTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGAATGCTTTTGTAGGcc                                          <  2:1081086/149‑1 (MQ=255)
                                                                                                            gTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGa                                       <  1:800608/149‑1 (MQ=255)
                                                                                                                 ccTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGcc                                                                                                              >  4:778038/1‑73 (MQ=255)
                                                                                                                 ccTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGcc                                                                                                              <  3:778038/73‑1 (MQ=255)
                                                                                                                  cTGCCGCTGCGCAGTAATCTTTTATGCCGGGTTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGc                                 <  3:994757/149‑1 (MQ=255)
                                                                                                                  cTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGc                                 >  1:345447/1‑149 (MQ=255)
                                                                                                                  cTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGc                                 <  3:343650/149‑1 (MQ=255)
                                                                                                                     ccGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGtt                              <  2:345447/149‑1 (MQ=255)
                                                                                                                      cGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGttt                             <  1:1076585/149‑1 (MQ=255)
                                                                                                                            gcAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTAcgccg                       >  3:493309/1‑149 (MQ=255)
                                                                                                                            gcAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTAcgccg                       >  1:10483/1‑149 (MQ=255)
                                                                                                                              aGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCa                     >  2:321193/1‑149 (MQ=255)
                                                                                                                              aGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCa                     >  3:299958/1‑149 (MQ=255)
                                                                                                                              aGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGAAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATGCGGCGTACAcgcctta                     <  4:1352291/149‑4 (MQ=25)
                                                                                                                                tAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATc                   >  4:1288981/1‑149 (MQ=255)
                                                                                                                                tAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATc                   >  3:306576/1‑149 (MQ=255)
                                                                                                                                  aTCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCa                                                                                           >  4:1387802/1‑75 (MQ=255)
                                                                                                                                  aTCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCa                                                                                           <  3:1387802/75‑1 (MQ=255)
                                                                                                                                         aTGCCGGGCATAGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATa                                     >  2:203563/1‑122 (MQ=255)
                                                                                                                                         aTGCCGGGCATAGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATa                                     <  1:203563/122‑1 (MQ=255)
                                                                                                                                         aTGCCGGGCATAGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTc          >  4:501497/1‑149 (MQ=255)
                                                                                                                                          tGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAttt           >  2:552677/1‑147 (MQ=255)
                                                                                                                                          tGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAttt           <  1:552677/147‑1 (MQ=255)
                                                                                                                                           gCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTcac        >  3:908577/1‑149 (MQ=255)
                                                                                                                                             cGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCacaa      <  3:1290203/149‑1 (MQ=255)
                                                                                                                                               ggCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCacaaca    >  4:153741/1‑149 (MQ=255)
                                                                                                                                                 cTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAg  >  3:630192/1‑149 (MQ=255)
                                                                                                                                                 cTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAg  <  3:657227/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GTGGGGAAGGTGGCGCAAAATCTGCAAGCGCGAAACCTGCAGAAAAACCGTCTCGCCGCCTCGGCGATGCCAAACCGGCAGGCGAACAACAACGTCGCCGCCGTCCGCGTAAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAG  >  NC_000913/832106‑832399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: