Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 850,996 C→A 5.5% intergenic (+31/+18) ompX → / ← opgE outer membrane protein X/OPG biosynthetic transmembrane phosphoethanolamine transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913850,9960CA 5.5% 640.3 / 16.7 179intergenic (+31/+18)ompX/opgEouter membrane protein X/OPG biosynthetic transmembrane phosphoethanolamine transferase
Reads supporting (aligned to +/- strand):  ref base C (75/94);  new base A (5/5);  total (80/99)
Fisher's exact test for biased strand distribution p-value = 7.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 181 total aligned reads displayed.

GGGTCTGCAGTTCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTT  >  NC_000913/850851‑851142
                                                                                                                                                 |                                                                                                                                                  
gggTCTGCAGTTCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCggg                                                                                                                                                 <  4:515925/149‑1 (MQ=255)
gggTCTGCAGTTCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCggg                                                                                                                                                 >  1:696391/1‑149 (MQ=255)
gggTCTGCAGTTCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCggg                                                                                                                                                 >  1:725464/1‑149 (MQ=255)
    cTGCAGTTCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCgg                                                                                                                                             <  2:775615/149‑1 (MQ=255)
     tGCAGTTCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCTGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGa                                                                                                                                            <  3:760835/149‑1 (MQ=255)
     tGCAGTTCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGa                                                                                                                                            >  4:804409/1‑149 (MQ=255)
        aGTTCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGAttt                                                                                                                                         <  1:378606/149‑1 (MQ=255)
         gTTCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGAtttt                                                                                                                                        <  3:228221/149‑1 (MQ=255)
          ttCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGAttttt                                                                                                                                       <  1:272381/149‑1 (MQ=255)
          ttCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGAttttt                                                                                                                                       <  3:1153464/149‑1 (MQ=255)
          ttCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCCCGAGAGGCGGAtttt                                                                                                                                        >  2:513381/1‑148 (MQ=255)
            cAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCCCGAGAGGCGGAtttttta                                                                                                                                     <  1:594107/149‑3 (MQ=255)
             aaCCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGAttttt                                                                                                                                       >  3:91010/1‑146 (MQ=255)
             aaCCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGtt                                                                                                                                    >  1:1086433/1‑149 (MQ=255)
             aaCCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGtt                                                                                                                                    >  1:1065121/1‑149 (MQ=255)
              aCCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGttt                                                                                                                                   <  2:1304067/149‑1 (MQ=255)
               cccGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGATCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGtttt                                                                                                                                  <  3:500051/149‑1 (MQ=255)
               cccGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGtttt                                                                                                                                  >  1:689490/1‑149 (MQ=255)
                  gATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTaa                                                                                                                               <  4:1213632/149‑1 (MQ=255)
                  gATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTaa                                                                                                                               >  4:1335159/1‑149 (MQ=255)
                  gATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTaa                                                                                                                               <  4:477596/149‑1 (MQ=255)
                    tGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAgg                                                                                                                             >  2:708036/1‑149 (MQ=255)
                      gAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGtt                                                                                                                           >  4:1127904/1‑149 (MQ=255)
                       aaaaCGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGAttttt                                                                                                                                       <  4:60537/136‑1 (MQ=255)
                         aaCGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCg                                                                                                                        <  2:1114695/149‑1 (MQ=255)
                           cGTTGCTCTGGTCTTCTCTTAAGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCggg                                                                                                                      <  1:519834/149‑1 (MQ=255)
                                   tGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAat                                                                                                              >  4:408508/1‑149 (MQ=255)
                                        ttCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTc                                                                                                                    <  1:184849/138‑1 (MQ=255)
                                        ttCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTc                                                                                                                    >  2:184849/1‑138 (MQ=255)
                                        ttCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCgg                                                                                                         >  3:931045/1‑149 (MQ=255)
                                         tctcTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGt                                                                                                        <  1:320224/149‑1 (MQ=255)
                                             ttACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCg                                                                                                          <  3:324128/143‑1 (MQ=255)
                                                 gAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGAt                                                                                                <  3:1335159/149‑1 (MQ=255)
                                                     agagCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGtt                                                                                            <  4:1180513/149‑1 (MQ=255)
                                                     agagCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGtt                                                                                            <  1:980011/149‑1 (MQ=255)
                                                      gagCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTg                                                                                           <  2:1201762/149‑1 (MQ=255)
                                                         ccGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTaa                                                                                                                               >  1:562742/1‑110 (MQ=255)
                                                         ccGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTaa                                                                                                                               <  2:562742/110‑1 (MQ=255)
                                                           gTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGt                                                                                                                     >  2:1424834/1‑118 (MQ=255)
                                                            tATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCg                                                                                                          >  4:1441566/1‑128 (MQ=255)
                                                            tATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCg                                                                                                          >  1:575850/1‑128 (MQ=255)
                                                            tATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATcc                                                                                     >  2:47882/1‑149 (MQ=255)
                                                                 gTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTg                                                                                           >  1:502696/1‑138 (MQ=255)
                                                                 gTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCtt                                                                                <  3:1038506/149‑1 (MQ=255)
                                                                  tAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTc                                                                               <  4:188502/149‑1 (MQ=255)
                                                                   aGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCt                                                                              <  4:765438/149‑1 (MQ=255)
                                                                             tAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGaa                                                                    >  2:1361646/1‑149 (MQ=255)
                                                                                 cACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTg                                                                <  3:86356/149‑1 (MQ=255)
                                                                                 cACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTg                                                                <  1:258015/149‑1 (MQ=255)
                                                                                  aCCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATc                                                                                      >  1:852319/1‑126 (MQ=255)
                                                                                  aCCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATc                                                                                      <  2:852319/126‑1 (MQ=255)
                                                                                  aCCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTgg                                                               <  4:1279924/149‑1 (MQ=255)
                                                                                  aCCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTgg                                                               >  3:872872/1‑149 (MQ=255)
                                                                                   ccTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGt                                                              <  4:355076/149‑1 (MQ=255)
                                                                                   ccTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGt                                                              <  4:872872/149‑1 (MQ=255)
                                                                                   ccTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGt                                                              <  2:1065121/149‑1 (MQ=255)
                                                                                   ccTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATACGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGACATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGt                                                              <  3:240657/149‑1 (MQ=255)
                                                                                    cTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTg                                                             >  3:441505/1‑149 (MQ=255)
                                                                                     tGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGa                                                            >  2:605784/1‑149 (MQ=255)
                                                                                       gATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGAttttt                                                                                                                                       <  2:230067/72‑1 (MQ=255)
                                                                                       gATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGAttttt                                                                                                                                       >  1:230067/1‑72 (MQ=255)
                                                                                         ttGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGta                                                        <  3:1280588/149‑1 (MQ=255)
                                                                                           gCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGt                                                              >  2:818381/1‑141 (MQ=255)
                                                                                           gCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGt                                                              <  1:818381/141‑1 (MQ=255)
                                                                                           gCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGtata                                                      <  2:1476978/149‑1 (MQ=255)
                                                                                            ccGGTGTTGGGTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCTACCTTCTGTAACTGGAAGTTGGTGATGTatat                                                     <  4:931045/149‑1 (MQ=255)
                                                                                                      ttACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCTGAGATGGTTGTAATCCACCt                                                                                 <  1:1178877/111‑1 (MQ=255)
                                                                                                      ttACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCt                                                                                 >  2:1178877/1‑111 (MQ=255)
                                                                                                      ttACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCt                                           <  2:1086433/149‑1 (MQ=255)
                                                                                                         ccGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTttc                                        <  4:1036381/149‑1 (MQ=255)
                                                                                                           gCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAGAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTttctt                                      <  1:1348430/149‑1 (MQ=255)
                                                                                                               cTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCa                                  <  1:405425/149‑1 (MQ=255)
                                                                                                                tAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAg                                 >  3:143077/1‑149 (MQ=255)
                                                                                                                 aaTCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGa                                >  4:579974/1‑149 (MQ=255)
                                                                                                                 aaTCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGa                                <  2:990101/149‑1 (MQ=255)
                                                                                                                  atgaCTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGat                               <  4:774165/146‑1 (MQ=255)
                                                                                                                  aTCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGat                               >  4:909982/1‑149 (MQ=255)
                                                                                                                   tCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGata                              >  1:505433/1‑149 (MQ=255)
                                                                                                                   tCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGata                              >  2:1268202/1‑149 (MQ=255)
                                                                                                                   tCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGata                              >  2:901464/1‑149 (MQ=255)
                                                                                                                      cTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATaa                           <  2:591947/149‑1 (MQ=255)
                                                                                                                       tttGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATaaa                          >  3:180523/1‑149 (MQ=255)
                                                                                                                          ggTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATTGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATTTATATCAGCCCGGCTCTCTTCTCAGATATAAAcag                       <  2:32453/149‑3 (MQ=255)
                                                                                                                          ggTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACgg                       <  2:29751/149‑1 (MQ=255)
                                                                                                                          ggTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACgg                       <  1:1361646/149‑1 (MQ=255)
                                                                                                                           gTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACggg                      >  3:1205905/1‑149 (MQ=255)
                                                                                                                               aaacaaaaaTCCGCCCCGAGAGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTtctc                                   >  1:145625/5‑132 (MQ=39)
                                                                                                                                ataAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTAttt                 >  4:295084/1‑149 (MQ=255)
                                                                                                                                ataAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTAttt                 <  1:871983/149‑1 (MQ=255)
                                                                                                                                 taAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGtt                                                                 <  4:223408/100‑1 (MQ=255)
                                                                                                                                 taAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTg                >  4:1024188/1‑149 (MQ=255)
                                                                                                                                     aaaTCCGCCCCGAGAGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGt            >  4:1398027/1‑149 (MQ=255)
                                                                                                                                      aaTCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGa               <  1:1409351/145‑1 (MQ=255)
                                                                                                                                      aaTCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGtt           >  1:643117/1‑149 (MQ=255)
                                                                                                                                       aTCCGCCCCGAGAGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTa          >  1:633766/1‑149 (MQ=255)
                                                                                                                                       aTCCGCCCCGAGAGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTa          >  2:689772/1‑149 (MQ=255)
                                                                                                                                            ccTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTc     <  4:449970/149‑1 (MQ=255)
                                                                                                                                            ccTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTc     >  3:555315/1‑149 (MQ=255)
                                                                                                                                              tctcGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCt   <  1:1296480/149‑1 (MQ=255)
                                                                                                                                               ctcGGGGCGGATTTTCGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCtt  <  3:405658/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GGGTCTGCAGTTCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATGTTAATTTCCTT  >  NC_000913/850851‑851142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: