BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,117,047	G→T	S59Y (TCC→TAC)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,117,047	0	G	T	97.7%	433.8 / NA	135	S59Y (TCC→TAC)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand): ref base G (1/2); new base T (60/70); total (63/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

CACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAA‑TTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAT  >  NC_000913/4116776‑4117318

cacaTGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTCCGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGTTTTCACGCTGGTTCGTAATCACGATAGCTGCAATTTGATCGGAACTGTTATCGGATTTCGCTAGCACTTCTACCAGCGTGTAGc                                                                                                                                                                                                                                                                               >  3:531632/1‑275 (MQ=255)

 caagggTCAAGGGTCCACTTCACTTTGGGGCCAGGAAAGTACGGGCCAATCACCAGACCGGTATTGCTGCGGTTATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCGGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCt                                                                                                                                                                                                                                                                              <  3:299388/271‑1 (MQ=255)

      gTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATGGATAGGCTTTCCGGTTTCTTTTTCCCAGACAATAATGGTTTCACGCTGGTTCGTAATACCCATAGCTGCAATTGGATCGGAACTGATATCGTCTTTCGCCAACACTTCTACCAGCGTTTAGCTTTgg                                                                                                                                                                                                                                                                          >  3:590752/1‑274 (MQ=255)

      gTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTggg                                                                                                                                                                                                                                                                         >  1:508742/1‑275 (MQ=255)

       tCGAGGATCCACTTCACTTTGGTGCCAGACAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTggg                                                                                                                                                                                                                                                                         <  2:658184/274‑1 (MQ=255)

          atgATCCACTTCACGTTGGGGCCATAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTGCTAACCCGTCACGTTTTAAATGCTCGCAGATGTCTGCGGTACGACGTCACTGGCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGAAAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGcc                                                                                                                                                                                                                                                                    <  4:517380/274‑1 (MQ=255)

           ggAACCACTTCACTTTGGGTCCAGAAAAGTACGGGTCAATCACCAGACCCGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTCTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGcc                                                                                                                                                                                                                                                                    <  1:85042/275‑1 (MQ=255)

           gaaTCCACTTCACTTTGGTGCCAGAAAAGTACGTGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGcc                                                                                                                                                                                                                                                                    <  1:531632/273‑1 (MQ=255)

                 acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCTCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTCCTTTTTCCCACACAATAGTGTTTTCACGCTGGTTCGTTCTACCTATAGCTGCCATTTGATCGGCACTGCTATCGGCTTTCGACACCACTTCTTCAAGCGTTAGGCTTTGGTTGGCCCACAttt                                                                                                                                                                                                                                                             >  4:187335/1‑276 (MQ=255)

                 acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCAAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTCCTACCAGCGTGTAGCTTTGGGTGGTCAAGactc                                                                                                                                                                                                                                                             >  2:498589/1‑273 (MQ=255)

                     cacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATTTCGGCTTTCGCACGCACTTCTACACGCTTGTTGCTTTGTGTGGTCCCGGTGTCCAt                                                                                                                                                                                                                                                         >  4:372270/1‑276 (MQ=255)

                        tttGGTGCCAGAAAAGTACTGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTGGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCAtt                                                                                                                                                                                                                                                        <  1:235786/274‑1 (MQ=255)

                           ggTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGt                                                                                                                                                                                                                                                    >  3:238555/1‑275 (MQ=255)

                             tGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGTTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTCCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGAGATCGGCTTTCGCCCGCACTTCTACCAGCCTGTAGCTTTGGGTGTCCCATAAGTCCTTTAGGTCg                                                                                                                                                                                                                                                  >  4:252135/1‑275 (MQ=255)

                             ggacataaaaGTCCGGGTCAATCAGCTGAACGGTGTTGCTGCGGATATGATCTTCTAAACCGTTACGTTTTCAAGGCTCACAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTGCCCATACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                                                                                                                                                                                                                   <  3:423507/268‑1 (MQ=255)

                               ccAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                                                                                                                                                                                                                               >  2:132198/1‑276 (MQ=255)

                                 aGAAAAGCACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAACCTTCTAAACCGTCACGCTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACCGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCAAGACAATAGTGGTTTCACGCGGGTTCGTAATACCGACAGCTGCAATTTGATCGGAACTGATATCGGCCTTCGCCAGCCATTCTACCAGCGTGTAGCTTTGGGTGGCCAGGATTTCCATTGGGttcggttc                                                                                                                                                                                                                                             >  2:187398/1‑269 (MQ=255)

                                  gAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGACCCGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGAACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAAGCGTGGTTTCACGCTGGTTCGTAACACCGATAGCTGCGATGTGATCGGAACTGATAGCGGCTTTCGCCACACCTTCAACATGCCGGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTc                                                                                                                                                                                                                                             >  3:240885/1‑275 (MQ=255)

                                       gTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTGTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATATATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTAcc                                                                                                                                                                                                                                         <  1:240885/274‑1 (MQ=255)

                                             gTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCCTTTTCTCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGAACTCCTACCAGCGTGTCGCTTTGGGTGGCACAGTTTTCCATTGGGTCGTGTTCTACCCAActggg                                                                                                                                                                                                                                 >  4:472453/1‑272 (MQ=255)

                                                                   ctgcGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGTCAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTTGTGTTCTACCCAATCTGGTTTTGGGTAGATTTGCTTaa                                                                                                                                                                                                             >  1:227032/1‑274 (MQ=255)

                                                                   ctgcGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGATTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTCGATCGGAACTGATATCGGCTTTCGCCAGCCATTCTACCAGCGTGTAGCTTTGGGTGGCCGAGATTTCCATTGGGTTGGTTTCATACCAACCTGGTTTTGGGTAGGTTTGCTCaa                                                                                                                                                                                                             >  3:486008/1‑274 (MQ=255)

                                                                       ggATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGTTGGCCCAGATTTCCATTTGGTCGTGTTCTACACAACCTGGTTTTGGGTAGTTTTTCTCACATTcgc                                                                                                                                                                                                       >  4:206600/1‑276 (MQ=255)

                                                                        gATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTTACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCTGTTAGCTTTGGGTGGCCCAGATTTCCCTTGGGTCGTTTTCTAACCAAACTGGTTTTGGGTAGGTTTGCTCACATTctct                                                                                                                                                                                                      >  4:45174/1‑273 (MQ=255)

                                                                        gATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGCCTTTCGCTAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCCGATTTCCATTGGGTCGTGTTCTATCCAACCTGGTTTTGGGTAGCTTTGCCCAAATTcgcg                                                                                                                                                                                                      >  2:356216/1‑276 (MQ=255)

                                                                             caTCTCCTAACACGTCAGGTTTTAAATGCGTGCAGATTTCGGCGGGACGACGGGACTGCCAGAAAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGAGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCg                                                                                                                                                                                                 <  4:241157/275‑1 (MQ=255)

                                                                              accttcgAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                                                                                                                                                                <  2:209555/274‑1 (MQ=255)

                                                                                cttctAACTCGTCACCTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCCCTGCCAGACATTGGCGTTATAGCTAGGCTTGCCGGTTTCTTTTTCCCATACAATATTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                                                                                                                                              <  4:701607/276‑1 (MQ=255)

                                                                                 ttctAACCCGTCACGTTTTAAATGCTCTACGTTTTCTTCGGTACGACGGCCCTGCCATCCAATTGCGTTATAGTTCGGCTTTCCCGTTTCTTTTTCCCAGACCATAGTGGTTTCCCGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                                                                                                                                              <  4:308025/275‑1 (MQ=255)

                                                                                  tctAAACCGTGACGGTTTAAATGCTCGCATATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACg                                                                                                                                                                                            <  4:564982/276‑1 (MQ=255)

                                                                                  tctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTTTAGCTTTGGGTGGCCCAGATTTCCCTTTGGTCGTGTTCTACCCCACCTGGTTTTGGGGAGCTTTGCTCAACTTCGCGCTGCGacac                                                                                                                                                                                             >  2:31388/1‑275 (MQ=255)

                                                                                  tctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTTTTCTACCCAACCTGGTTTTGGGTAGATTTGCTAAAATTCGCGGTGCGACACg                                                                                                                                                                                            >  2:688520/1‑276 (MQ=255)

                                                                                   ctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCCATTTGATCGGAACTGAAATCGTCTTTCTCCAGCACTTCTCCCAGCTCGCAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCCACCCAACTTGGCTTTGGGTAGTCTTTCTCTAATTCACCCTGTGacatc                                                                                                                                                                                            >  3:582453/1‑273 (MQ=255)

                                                                                   ctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTTGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCCGGGTTTGGGTAGATTTGCTCAACTTCGCGCTGCGACACg                                                                                                                                                                                            >  4:112709/1‑275 (MQ=255)

                                                                                   ctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTc                                                                                                                                                                                                         >  2:437257/1‑262 (MQ=255)

                                                                                    tAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCt                                                                                                                                                                                          <  2:454286/276‑1 (MQ=255)

                                                                                      aaCCTTCACGGTTTAAAGGCTCGCGGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTcgcg                                                                                                                                                                                                      <  4:437257/262‑1 (MQ=255)

                                                                                      aaCCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                                                                                                                                                                         <  2:112709/275‑1 (MQ=255)

                                                                                      aaCCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                                                                                                                                                                         <  2:372270/275‑1 (MQ=255)

                                                                                       aacGTCACGTTTTAACTGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                                                                                                                                                                         <  1:590752/272‑1 (MQ=255)

                                                                                           tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATgct                                                                                                                                                                                    >  2:25535/1‑273 (MQ=255)

                                                                                           tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGat                                                                                                                                                                                    >  2:513846/1‑275 (MQ=255)

                                                                                           tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCGCCAATTCGCTCTGCGAGCCGCTCAGGat                                                                                                                                                                                    >  3:356272/1‑275 (MQ=255)

                                                                                             aCGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCCGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACCCGCTacggatttt                                                                                                                                                                                 >  2:314864/1‑268 (MQ=255)

                                                                                             aCGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTGCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTAGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCCGCACTTCTACTAGCGTGTAGCTTTGGGTGGCCCAGAGTTCCATTGGGACGTGTTCTACCCGACCGGGTGTTGGGTAGATTTGCTGAGATTCGGGCTGCGACCCGCTAATGat                                                                                                                                                                                    >  2:16008/1‑273 (MQ=255)

                                                                                                       ttcTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                                                                       <  2:12968/274‑1 (MQ=255)

                                                                                                       tGCTCGCAGATTTTTGCGGTACGCCGGCACTGCCAGGCAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGTCAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGCTCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                                                                       <  2:149268/276‑1 (MQ=255)

                                                                                                       tGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCGGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACATGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                                                                       <  2:257196/276‑1 (MQ=255)

                                                                                                       tGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                                                                       <  2:206600/276‑1 (MQ=255)

                                                                                                       ggtgtgCAGCTTGCTGCGTTACGCACGCAATGCAAGACAATGGCGTTATGGTGAGGCTTGCCGGGTTATCTTTCCCGGACAAGAGTGGTTTGACGCTGATGCGTAATACCGATAGCTTTAATTGCATCGGAACTGGTAGCGGCTTTAGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTGTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                                                                       <  4:468502/271‑1 (MQ=255)

                                                                                                        ttatgccgATTTGCGCGGGTCGACGGCACTGCCAGACGATGGCTGCATATATGGGCTTGTCGATTTCTTTTTCCCAGACAATAGTGGTTACGCGCTGGGTCGTAATACCGTCAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTGCCAGCGTGTAGCTTGGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCGAATTCGCGCTGCGAAACGCTAATGATCTTGGCAGCGTGa                                                                                                                                                                       <  3:125160/268‑1 (MQ=255)

                                                                                                        acTCGCGGATTGCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCGTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATTTCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                                                                       <  1:586858/274‑1 (MQ=255)

                                                                                                                            cgacgGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTCCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATATCTGCCATTTGTTCGGAACTGATATCGGCTTTCCCCAGCCCTTCTCCCAGCGTGTAGCTTCGGGTGTCCCAGATTTTCATTGGTTCGGGTTTTACCCAACCTGGTTTTGGCTTGATTTGCTCAAATTCCCGCTTCTATCCGCTCATGACTTTGTCATCCTTGTCAATTTCCACCGCGCgggg                                                                                                                                                   >  4:233046/1‑274 (MQ=255)

                                                                                                                              acgGCACTGGCAGACAATGGCGTTATAGATAGGTTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGc                                                                                                                                                 <  2:252135/275‑1 (MQ=255)

                                                                                                                               cgGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAAtt                                                                                                                                                                                                          >  3:310675/1‑217 (MQ=255)

                                                                                                                               cgGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGc                                                                                                                                                 <  1:21628/274‑1 (MQ=255)

                                                                                                                                   actcgcAGACAGTGGGGTTTTGGCTAGGCTTGCGTGTTTGTGTTTCCCAGACAAAAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTTCAATTTGAGCGGAACCTATATCGGGTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg                                                                                                                                            <  3:171766/270‑1 (MQ=255)

                                                                                                                                   aCTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTcgcg                                                                                                                                                                                                      <  1:310675/217‑1 (MQ=255)

                                                                                                                                    cTGCCAGACAATTGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtgg                                                                                                                                           <  1:576717/275‑1 (MQ=255)

                                                                                                                                    cTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCACAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCCAATGATATTGGCAGCGTGTTCCTTTTCTACCGCGTggggcgtggtgtg                                                                                                                                          >  4:6941/1‑266 (MQ=255)

                                                                                                                                             aaTGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTc                                                                                                                                 <  2:6941/276‑1 (MQ=255)

                                                                                                                                               tGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACCCGCTAATGATATTTGCCTCGTGATCCATTACTACCGGGCGGGCGCTGGTGGTTCCCTggg                                                                                                                                  >  3:395174/1‑272 (MQ=255)

                                                                                                                                                  ggTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTTGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAgcg                                                                                                                            <  2:472453/275‑1 (MQ=255)

                                                                                                                                                    ttATAGATTGGCTTGCCAGTTTCTTTGTCCCCGACAATTGTGGTTACACGTTGGTTACTAATTCCGATAGCTGCAATTTTATCGGAACTAATATCGGCTTTCGCCAGCAGTTCTACGAACGTGTAGCTTTGGGTGGCCCAGAGTTCCCTTGGGTCGTGTTCTGCCCAACCTGGTTTTGGGTAGATTTGCTCAGATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTGCGACCGCGCGGGAGCTGGTGGTGCCCTAGTCGAGCGCa                                                                                                                          <  4:187398/276‑1 (MQ=255)

                                                                                                                                                       tagatagGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGACCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCTCGCGGGAGCTGGTGGTGGCCTGGTCGAGCGGAAc                                                                                                                        >  2:424096/1‑275 (MQ=255)

                                                                                                                                                          gttgtcTTGGCGGTTTGTTGTTCCAAGACCTATGTGGTTTCACGCTGGTTCGGAAGACCTATATCTTAAATTTGATCGGAATGTATATCGGCTTTCGCCAGCACTTCTACCAACGGGTAGCTTTGGGTGGCCCAGTTTGCCATTGGTTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGata                                                                                                                    <  4:425755/271‑1 (MQ=255)

                                                                                                                                                                 tGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTAccc                                                                                                                                                                                                                                        >  2:274542/1‑153 (MQ=255)

                                                                                                                                                                  gCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACg                                                                                                                                                                                            >  2:533290/1‑196 (MQ=255)

                                                                                                                                                                    cGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAc                                                                                                                                                                                                                                     <  4:274542/153‑1 (MQ=255)

                                                                                                                                                                     ggTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                                                                                                                                                                         <  4:533290/196‑1 (MQ=255)

                                                                                                                                                                      gTTTTTTTTACCAAGGGAATAGTGGTTGTACGCTGGTTGGTAATACCGATAAGTGCAATTTGATCGGAACTGGTATCGGCTTTCGCCAGGAGTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTGCCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCT‑GTCGAGCGCAACGATATATTTTTTTTCAg                                                                                                       <  4:132198/276‑1 (MQ=255)

                                                                                                                                                                                  cAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTaa                                                                                             <  1:395174/275‑1 (MQ=255)

                                                                                                                                                                                       gaTAGTGTTTTAACGCTGGTTAGTAATACCGATAGCTGAAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACATGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAA‑TTGTc                                                                                       <  2:213706/274‑1 (MQ=255)

                                                                                                                                                                                         tAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCCGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAA‑TTGTtcc                                                                                     >  1:313739/1‑275 (MQ=255)

                                                                                                                                                                                          ttgtgTTTCACCGTGGTTCGTAATCCCGATACGTGCAATTTGTTCGGAACTGATATCGGCTTTGGCCAGCACTTCTACCATCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAA‑TTGTCCCGt                                                                                   <  4:314864/272‑1 (MQ=255)

                                                                                                                                                                                          cgTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTg                                                                                                                                                                                                                                                       >  1:418974/2‑113 (MQ=255)

                                                                                                                                                                                          aGTGGTGTGCCGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAA‑TTGTCCCGt                                                                                   <  4:132093/276‑1 (MQ=255)

                                                                                                                                                                                             ggTTTCACGCTGGTTCGTAATGCCCATAGCTTCAATTTGGTCGGAACTGATAGCGGCTTTCGCCAGCACTTCTGCCAGCATTTAGCTTTGGGTGACCCAGATTTCCCATGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAA‑TTGTCCCGTAg                                                                                 <  3:528723/275‑1 (MQ=255)

                                                                                                                                                                                              gTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCCTGTTTAA‑TTGTCCCGTAg                                                                                 >  1:181907/1‑274 (MQ=255)

                                                                                                                                                                                               tttCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAA‑TTGTCCCGTAGTCa                                                                              >  2:295874/1‑276 (MQ=255)

                                                                                                                                                                                                 tCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCTAACCTGGTTTTGGGGAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATCGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAA‑TTGTCCCGTAGTCat                                                                             >  1:510657/1‑275 (MQ=255)

                                                                                                                                                                                                   aCGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGTAGCTGGTGGTGCCCTGGTCGACGGCAACGATATATTTTTTTTCCGTTCTGTTTTA‑TTTTCCCGTAGttttact                                                                          >  4:213706/1‑270 (MQ=255)

                                                                                                                                                                                                   aCGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCTTGTTTAA‑TTGTCCTGTGGTCattt                                                                           >  1:421750/1‑273 (MQ=255)

                                                                                                                                                                                                         gTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAACTTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCCAGCGTCCAGCTATATTTTTTTTCAGTCATGTTTAA‑TTGTCCCGTAGTTATATTAcc                                                                       >  3:298103/1‑272 (MQ=255)

                                                                                                                                                                                                          ttCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAA‑TTGTCCCGTAGTCATATTACAGCg                                                                    <  2:8934/275‑1 (MQ=255)

                                                                                                                                                                                                                   ccGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCACATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCCTTACGACCGCGCGGGCGCTGGTTGTGTCTTGGTCGAGCGCCACGATATATTTTTTTTCGTTCTTGTTTAAGTTGT‑CAGTTGTCATGTTTCACCGAAGGttttgt                                                          >  4:578864/1‑274 (MQ=255)

                                                                                                                                                                                                                      aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTTCCCTGGTCGGCCGCAACGATATCTTTTTTTTCAGTCATGTTTAA‑TTGTCCCGTAGTCATATTACTGCGAAGCTTTTTGtt                                                        >  1:246634/1‑275 (MQ=255)

                                                                                                                                                                                                                      aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAA‑TTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGtt                                                        >  3:174136/1‑275 (MQ=255)

                                                                                                                                                                                                                         gCTGCAATTTGATCAGAGCTGATATCGGCTTTCGCCAGCACTTCTACAAGCATGTAGCTGTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAA‑TTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGa                                                    <  2:187335/276‑1 (MQ=255)

                                                                                                                                                                                                                           tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAA‑TTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTTTGa                                                    >  1:570548/1‑274 (MQ=255)

                                                                                                                                                                                                                            gCAATTTGATCGGAACTGATATCGGCTTTCGCCTGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAA‑TTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAg                                                  <  2:271997/275‑1 (MQ=255)

                                                                                                                                                                                                                                         aaCTGATATCGGCTGTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAGCCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAA‑TTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGtt                                       <  1:298103/273‑1 (MQ=255)

                                                                                                                                                                                                                                              atatCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtg                                                                                                                                               >  2:670896/1‑165 (MQ=255)

                                                                                                                                                                                                                                              atatCGGATTTCGCAATACGTTCTACTAGCGTGTAGATTGTGGTGTCCCAGATTTCCATGGGTTCGTGTACTACCCAACCTGGTTTTGGGTAGATTTGCTCAACTTGGCGCTGCGACACGGTAATGATGTTGGCATCGTGGTCCAGTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCTAGCGCAACGATATATTTTTTTTCAGTCATGTTTAA‑TTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCtt                                  <  3:227032/273‑1 (MQ=255)

                                                                                                                                                                                                                                                 tCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg                                                                                                                                            <  4:670896/165‑1 (MQ=255)

                                                                                                                                                                                                                                                    gCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAA‑TTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCcac                         <  2:560826/276‑1 (MQ=255)

                                                                                                                                                                                                                                                        tCGCCAGAACTTTTACCAGCGTGTAGTTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAA‑TTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCacaac                      <  3:480876/275‑1 (MQ=255)

                                                                                                                                                                                                                                                                    cTACCAGCGTGTAGCTTTGGGTTGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAA‑TTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGc           <  1:174136/274‑1 (MQ=255)

                                                                                                                                                                                                                                                                          gCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAA‑TTGTCCCGTAGTCATATTCCAGCGAAGCTTTTTGTTCTGAAGGAGTTTTGGTTTCTTTTTCTGCCACAACACATATATCGCAAGGCaa    >  3:523648/1‑275 (MQ=255)

                                                                                                                                                                                                                                                                            gtgtAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAATCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAA‑TTGTCCAGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGGTGTTGTTTCCTTTTCCTCCACAACACAGATATCGCAAGGCAAAt  >  1:183050/1‑275 (MQ=255)

CACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAA‑TTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAT  >  NC_000913/4116776‑4117318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`