Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,622,348:1 | +A | 5.1% | coding (115/639 nt) | purN → | phosphoribosylglycinamide formyltransferase 1 |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,622,348 | 1 | . | A | 5.1% | 1068.9 / 53.4 | 255 | coding (115/639 nt) | purN | phosphoribosylglycinamide formyltransferase 1 |
Reads supporting (aligned to +/- strand): ref base . (124/118); new base A (6/7); total (130/125) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.82e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-03 |
Only 100 of 259 total aligned reads displayed.
CACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGAAAATCGGTATCATCAAAGCCTCTGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGCCGGGCGTTTGCTGAACATTCACCCTTCTCTGCTGCCGAAATATCCCGGATTACACACCCATCGTCAGGCGCTGGAAAATGGCG > NC_000913/2622084‑2622618 | cACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTACTCAATGCCAACGGTGAAAACGCGTGGAAAATCGGTATCATCAAAGCCTCTGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGAGTTCg > 2:113255/1‑274 (MQ=255) cAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGAAAATCGGTATCATCAAAGCCTCTGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTCTTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACCCATACGCTCATCCCCAgcac > 4:357449/1‑273 (MQ=255) cTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGAAAATCGGTATCATCAAAGCCTCTGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGa < 2:94348/274‑1 (MQ=255) aCGGTGAAAACGCGTGGAAAATCGGTATCATCAAAGCCTCTGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGAcc > 2:27934/1‑275 (MQ=255) gAAAACGCGTGGAAAATCGGTATCATCAAAGCCTCTGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGa < 2:484663/274‑1 (MQ=255) atcaAAGCCTCTGATTCCGAAAAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTa < 2:332900/274‑1 (MQ=255) atcaAAGCCTCTGATCCCTAACAATGCGTGGTTATCGAATATAGAATATTGTGGGGCTTATGTCCGGAAACGGAAGGAAGTAACAGGTAATTATTGACGCCTGTAAAAACAACAAAATTAAAGGCACCGTACGGGCAGGTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTGGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGAGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTa < 4:234663/274‑1 (MQ=255) ctctGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑a > 8:277693/1‑149 (MQ=255) tctGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑Ac > 6:800469/1‑149 (MQ=255) ctGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGAtgt > 1:159492/1‑276 (MQ=255) tGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTTCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCCGTCTTAAGCAATCAGCCAG‑Aca > 4:552880/1‑147 (MQ=255) gATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑Acgcg > 8:1002829/1‑149 (MQ=255) ttCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGtt > 5:1202395/1‑149 (MQ=255) tCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTc < 6:961083/149‑1 (MQ=255) tCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTc < 8:1482493/149‑1 (MQ=255) tCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCa < 2:541559/263‑1 (MQ=255) cGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCgg > 7:1251353/1‑149 (MQ=255) gAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGc > 8:1107569/1‑149 (MQ=255) caacGTGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGAACGCG‑TCGGCCtt < 7:371796/149‑1 (MQ=255) caacGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCtt < 7:1107569/149‑1 (MQ=255) aacGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGAACGCG‑TCGGCCtta < 7:275647/149‑2 (MQ=255) gcgTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTg > 7:653542/1‑146 (MQ=255) ggTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGACGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACgcgc < 7:1453024/149‑1 (MQ=255) aTCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCAt > 2:37010/1‑227 (MQ=255) ataatGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGc > 7:222184/1‑149 (MQ=255) aatGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCgg < 6:1046181/149‑1 (MQ=255) atGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCGGGCTGGTTTTATGCGCATtct > 2:625039/1‑275 (MQ=255) tGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGt > 6:1532821/1‑149 (MQ=255) tGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGt > 5:833344/1‑149 (MQ=255) gAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACTCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTa < 7:877654/149‑1 (MQ=255) tatTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGAACGCG‑TCGGCCttat < 5:309204/126‑3 (MQ=255) tatTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCgg < 6:1641138/143‑1 (MQ=255) tatTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTg > 7:435309/1‑149 (MQ=255) tgtgGTGCNTATTTCCGNNAACGGAAGTAATTTACAGGCAATNATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGc < 3:64881/147‑1 (MQ=255) gtggtgCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAAc < 6:1202395/149‑1 (MQ=255) gtgCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAAcg < 4:655936/122‑1 (MQ=255) gtgCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCa > 6:1504643/1‑149 (MQ=255) tgCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCAt > 8:704770/1‑149 (MQ=255) ttATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCt < 8:454175/112‑1 (MQ=255) tATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTg < 4:186582/176‑1 (MQ=255) ttCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCCACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATTTACGCACCCGATGTGTTCGGTCTGGCTGGTTTTATGCGCATTTTCAGCCCGGCGTTTGtccc > 4:433231/1‑271 (MQ=255) ccGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATc < 8:577716/149‑1 (MQ=255) ggCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGc > 5:202092/1‑149 (MQ=255) ggCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGtc < 2:433235/267‑1 (MQ=255) gCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGcc > 6:369709/1‑149 (MQ=255) cAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATc > 2:689566/1‑145 (MQ=255) cAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCa < 8:1251353/149‑1 (MQ=255) aaCGGAAGTAATTTACAGGCAATTATTGACGCCTGT‑AAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAgc > 6:996770/1‑149 (MQ=255) cGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATa < 2:212459/134‑1 (MQ=255) aaGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGt < 5:1528444/149‑1 (MQ=255) aaGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGCCGGGc < 2:657193/275‑1 (MQ=255) tAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTg > 8:950132/1‑149 (MQ=255) aaTTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGa > 6:297827/1‑149 (MQ=255) aTTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGAc > 5:784633/1‑149 (MQ=255) tttACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACa < 5:1622087/149‑1 (MQ=255) tttACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACa < 6:1363177/149‑1 (MQ=255) gCAATTATTGACGCCTGTAAACCCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGAACGCG‑TCGGCCttatt > 6:58568/1‑84 (MQ=38) gCAATTATTGAAGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGa > 7:958947/1‑140 (MQ=255) cAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGaa > 6:1613086/1‑149 (MQ=255) aCGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGtt > 1:44082/1‑67 (MQ=255) ccTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCgg < 8:605901/149‑1 (MQ=255) ccTGAAAAACCAACAAATTTAAAGGCACATTACGGGCAGTTTACAGTAATAAGGCAG‑ACGCGTTCGGCCTTGCACGCTGCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACACTCGTGAAGCCTATGCCCgg < 6:532650/149‑1 (MQ=255) cTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCtt > 5:494494/1‑70 (MQ=255) tGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGa > 8:421587/1‑149 (MQ=255) tGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACg < 1:185860/175‑1 (MQ=255) aaCCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATAGCCAGCGCGTTTGACAGTCGTGAAGCTTATGACCGGGAGTTGa < 8:39637/149‑1 (MQ=255) aacaaAATTAAGGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCa > 7:1352464/1‑149 (MQ=255) acaaAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATAc > 6:1301407/1‑94 (MQ=255) acaaAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGa < 7:856916/139‑1 (MQ=255) acaaAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGt > 6:869250/1‑141 (MQ=255) acaaAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGt < 5:869250/141‑1 (MQ=255) acaaAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCAt > 8:1325388/1‑149 (MQ=255) aaaaTTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGa < 7:500762/149‑1 (MQ=255) aaaaTTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGa > 5:621488/1‑149 (MQ=255) aaaTTAAAGGCACCGTACGGGCAGTTTTCATCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGt < 8:1160664/119‑1 (MQ=255) aaaTTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGt > 7:1160664/1‑119 (MQ=255) aaTTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATa < 4:749726/89‑1 (MQ=255) ttAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATc > 7:719542/1‑149 (MQ=255) ttAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATc < 8:1640225/149‑1 (MQ=255) caagggtgccGTACGGGTAGTTTTTAGCAATAGNCCCG‑GCGCGTTCGGCCTTGACCGCGNCCGCCCAGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCNTGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGCCGGGCGTTTGCTGAACATTCACCCTTCTCTGCTGCCGAAATATCCCGGATTaca < 3:163085/271‑1 (MQ=255) ggCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAg > 5:433599/1‑129 (MQ=255) ggCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAg < 6:433599/129‑1 (MQ=255) ggCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGtt > 8:1432830/1‑131 (MQ=255) ggCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACAt > 6:664237/1‑149 (MQ=255) tACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCa > 8:1585324/1‑149 (MQ=255) aCGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCAc < 5:1349763/149‑1 (MQ=255) cAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGAt < 5:59160/149‑1 (MQ=255) cAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGAt < 7:1629710/149‑1 (MQ=255) ttttCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGAtgtg < 6:855492/149‑1 (MQ=255) aGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGAtt < 8:870714/114‑1 (MQ=255) cAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTgc < 8:1083533/149‑1 (MQ=255) aaTAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTgct > 5:1011076/1‑149 (MQ=255) aTAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTgctg > 6:762798/1‑149 (MQ=255) tAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTgctgg > 7:1631496/1‑149 (MQ=255) tAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTgctgg < 5:996770/149‑1 (MQ=255) aGGCCGAACGCG‑TCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTgctggct > 8:31090/1‑149 (MQ=255) aGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTgctggct > 7:1070983/1‑149 (MQ=255) ggCCC‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGCATTGCAACGCATACGCTCCTCGCCCGCGCCATTGACAGCCGTGAAACCTATGACCGGGAGTTGATTCATGACATCGACATGCACGCACCCGATGTGGTCGTGCGCGCTTGCTTTATGCGCATGCTCAGCACGGCGTCTGTCTCCCACCATACCGAGCGTTTGATGCACAGTCCCCCTTCTCGGCTCCCGACATCACCCGGTTTCCATACCCATCCTCATGCGCTGGAGATTGGca > 4:517341/1‑274 (MQ=255) cG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGtt > 5:901946/1‑149 (MQ=255) g‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGttt > 5:1051998/1‑149 (MQ=255) | CACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGAAAATCGGTATCATCAAAGCCTCTGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCG‑ACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGCCGGGCGTTTGCTGAACATTCACCCTTCTCTGCTGCCGAAATATCCCGGATTACACACCCATCGTCAGGCGCTGGAAAATGGCG > NC_000913/2622084‑2622618 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |