Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,999,231 | T→A | 6.7% | T84S (ACT→TCT) | dcyD ← | D‑cysteine desulfhydrase, PLP‑dependent |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,999,231 | 0 | T | A | 6.7% | 742.4 / 31.3 | 223 | T84S (ACT→TCT) | dcyD | D‑cysteine desulfhydrase, PLP‑dependent |
Reads supporting (aligned to +/- strand): ref base T (101/102); new base A (8/7); total (113/110) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.51e-01 |
Only 100 of 227 total aligned reads displayed.
GCGCACTCTCCACATAACCTAGCGCGCCAAGAGCATTAGAACCGCCAACCGGAATGACATACGGGCGAAAGCCTTGTGCTTCGACTCGCGTCGCCAGCTCTTCCAGTTGGGCATTGGGATCGGTCAGTGCGTCGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTCCGAT > NC_000913/1998961‑1999493 | gcgcACTCTCCACATAACCTAGCGCGCCAAGAGCATTAGAACCGCCAACCGGAATGACATACGGGCGAAGGCCTTGTGCTTCGACTCGCGTCGCAAGCTCTTCCAGTTGGGCATTGGGATCGGTCAGTGCGTCGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGAAGTGCAGACCGAGTAGGGAAGCGACTGCGGGAGTCTg > 2:138779/1‑274 (MQ=255) ccctccgcacggaCCTAGCGCGACCAGTGCATTACAACCGCCAACCGGAATGAAAGCCGGGCGAAAGCGTGGGTGTTGGAGTGGCGTCGCCGGGTCGGCGAGTTGGGCATTGGGATCGGTGAGTGCGTCGCACATTGCAAGTTGGGAAGTGAACGGAGCCAGCAACAAACGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGGCAATAGGATTTTCCAGCAGCGCCACGCAGAGCAGACCGAGTTTGGCGGCGACTGCGGCAGTCTGCCGca < 4:191895/263‑1 (MQ=255) ccctcCACATCAACTAGAGCGCCACAGGCATTCGACCCGCCAACCCGAATTACATACGGGCGAAAGCCTTGTGCTTCGACTCGCGTCGCCAGCTCTTCCAGTTGGGCATTGGGATCGGTCAGTGCGTCGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATgg < 1:234308/277‑1 (MQ=255) aGCGCGCAAAGAGCATTAGAACCGCCAACCGGAATGACATACGGGCGAAAGCCTTGTGCTTCGACTCGCGTCGCCAGCTCTTCCAGTTGGGCATTGGGATCGGTCAGTGCGTCGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGc < 2:3554/255‑1 (MQ=255) cgcgcCAAGAGCATTAGAACCGCCAACCGGAATGACATACGGGCGCAAGCCTTGTGCTTCGACTCGCGTCGCCAGCTCTTCCAGTTGGGCATTGGGATCGGTCAGTGCGTCGCACATTTCGATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAAGAGGATTTTCCAGCAGCCCCACGCCGTGCAGCCCGAGTTTGGCCGCCACTGCGGCAGTCTTCCGCACCATGTTAGACTggtacc > 4:388194/1‑271 (MQ=255) ggCGAAAGCCTTGTGCTTCGACTCGCGTCGCCAGCTCTTCCAGTTGGGCATTGGGATCGGTCAGTGCGTCGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGca < 2:65241/216‑1 (MQ=255) gAAAGCCTTGTGCTTCGACTCGCGTCGCCAGCTCTTCCAGTTGGGCATTGGGATCGGTCAGTGCGTCGCACATTTCAATCTGGGTATTGAACAGATCCCGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATACGCGTATCCGCCCCTTTACGCagggacacc > 4:636952/1‑268 (MQ=255) tgtgCTTCGACTCGCGTCGCCAGCTCTTCCAGTTGGGCATTGGGATCGGTCAGTGCGTCGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCCCGCAGAGCATCTGCCGCgag > 2:674875/1‑275 (MQ=255) tGGGCATTGGGATCGGTCAGTGCGTCGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTGGGTTAGATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGAGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGGCGGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTCACGGCCCCTTCACGCATAGCGGCGCACGCGAGGAATTCCCGCTTCCGTCATTTATTGACCCCCAt > 4:581244/1‑275 (MQ=255) gTCAGTGCGTCGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGt > 6:1452183/1‑149 (MQ=255) tCGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGcac > 7:1223829/1‑149 (MQ=255) tCGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGccc < 2:414699/169‑1 (MQ=255) cGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGcaca < 8:2328/149‑1 (MQ=255) cGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTCCGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCg > 2:329039/1‑274 (MQ=255) acaTTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGGCGCACATgg < 5:39475/149‑1 (MQ=255) acaTTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATgg < 5:1682405/149‑1 (MQ=255) ttCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAg > 7:1109636/1‑149 (MQ=255) tCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGa < 7:1500250/149‑1 (MQ=255) aaTCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACt > 6:903984/1‑149 (MQ=255) tCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCTGCAGTCTGCCGCACATNGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCACAGCATCACCCGCGCGAATTTCCCNCTACAGTAATTTCTTACCGCCCATTCACACNNGGCTGACGTAATCCCGTTTCATCAACAttt > 3:110728/1‑276 (MQ=255) gggTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATc < 7:693890/149‑1 (MQ=255) gggTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATc < 5:53131/149‑1 (MQ=255) gTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGc < 7:17480/149‑1 (MQ=255) aagAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGcccc < 8:738628/147‑1 (MQ=255) aaCAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGc > 6:1486668/1‑149 (MQ=255) aaCAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGc > 5:600591/1‑149 (MQ=255) aTCCAGCCACAAGCGATTGCCTTTGGTTAAAGAGTTTTCTGGGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGGGTTTGGCAGCGACTGAGGCAGTCTTCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAAt < 4:348786/275‑1 (MQ=255) aTCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTaa > 5:340247/1‑149 (MQ=255) aTCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTaa < 5:734059/149‑1 (MQ=255) aGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAg > 7:338198/1‑149 (MQ=255) aGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAg > 6:1672957/1‑149 (MQ=255) gCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGccc > 1:216338/1‑135 (MQ=255) gCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGccc < 3:216338/135‑1 (MQ=255) gCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGc < 6:574128/149‑1 (MQ=255) gCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCt > 2:32150/1‑195 (MQ=255) aagagtgtGCGGTTGGTTAAATGGTTTTCTGGGGGTGTGCCGAAAGGATTTTCCAGCAGGGACACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGAACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAgcg < 4:502863/268‑1 (MQ=255) aaGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATc > 8:1680548/1‑149 (MQ=255) aaGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATc < 8:918752/149‑1 (MQ=255) aaGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATc > 5:1238992/1‑149 (MQ=255) ttGTCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCAcc > 6:211482/1‑149 (MQ=255) gTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTgg > 8:1113451/1‑112 (MQ=255) ggTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCa > 7:572593/1‑149 (MQ=255) ttAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGTCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCaga > 8:1057549/1‑149 (MQ=255) ttAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCaga > 8:635383/1‑149 (MQ=255) ttAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCaga > 8:487248/1‑149 (MQ=255) aaaTAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGc < 7:818159/149‑1 (MQ=255) tAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATc > 5:1014787/1‑149 (MQ=255) gTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATc < 5:1507389/118‑1 (MQ=255) tttCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCAc < 6:950592/135‑1 (MQ=255) ttCTGCGGTTGTTCCAATAGGATTTTCCAGCAGGGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGgcg < 2:265735/275‑1 (MQ=255) tGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAAAGCATCTGCCGCga < 7:128605/149‑1 (MQ=255) gTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGaaa > 7:1600153/1‑149 (MQ=255) tgCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTcc > 7:620335/1‑149 (MQ=255) cAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCTCGCAGAGCATCTGCCGCGAGAAATTCCAGc > 6:1038713/1‑149 (MQ=255) aaTAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTa < 5:908524/76‑1 (MQ=255) aaTAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATc > 8:1312432/1‑85 (MQ=255) tAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTa > 8:1499047/1‑149 (MQ=255) aGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGACATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGCGCGGCGTTGGCGCGCCGATAAACTCCAGCAGTgg > 1:153510/1‑279 (MQ=255) gATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCa > 7:1685027/1‑141 (MQ=255) gATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCa < 8:1685027/141‑1 (MQ=255) cagcagCGCCACGCAATGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTAt > 8:1616344/1‑149 (MQ=255) agcagcGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTAtt > 5:1122271/1‑149 (MQ=255) agcagcGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTAtt > 7:209620/1‑149 (MQ=255) agcagcGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTAtt > 6:1084259/1‑149 (MQ=255) gcagcGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACAt > 5:1529824/1‑57 (MQ=255) gcagcGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACAt < 6:1529824/57‑1 (MQ=255) ccgcgcCACGCAGGGCAAACCGAGTTTGGCATCGACATCGGCATGAGTACGAAAATGGTTGAACTGGGTAGCCCCGGCAGTAATAAGCGTATCTGCACCTTAACGCAGAGCATCTGCCGCGAGAAATGCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACggg < 4:329035/273‑1 (MQ=255) cagcGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTgc < 5:279069/149‑1 (MQ=255) tgagtccgggcgggCAGATCGGGTTTGGGGTCGCGTGGGGCAGTCTGCTGCCAATGGTTAGACTGGATCGGCCCGTAAGGAATAACCGGATATGCACATTCACGCAGGGGATCTGCCGCGAGAAATTCCAGCGTACGTAATTTATTGCAGCCCATGGCCATGGGGGGGACGTAATCCCTTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACggg < 4:422200/261‑1 (MQ=255) agcGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTgcc < 5:1084259/149‑1 (MQ=255) gcCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGTCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTgccgcc < 8:1143509/149‑1 (MQ=255) ccACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGccc < 5:1224991/149‑1 (MQ=255) cGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCAtt < 5:115469/149‑1 (MQ=255) acAGTGCAGACCGAGTTTGGGAGCGACTGCGGCAGTGTGCCGAAAATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTTCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTg > 8:108092/2‑149 (MQ=255) tGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCAt < 8:572593/149‑1 (MQ=255) tGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCAt < 6:639459/149‑1 (MQ=255) tGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCAt > 6:479842/1‑149 (MQ=255) cAGACCGNGTTTGGCGNCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCa < 3:54991/146‑1 (MQ=255) gACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATgggg < 5:685330/149‑1 (MQ=255) aCCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGATTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGTTAATCAGAAAAGCGCGGCAGATCTTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTCTGCAGTGGCa > 2:455962/1‑275 (MQ=255) ccGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGt > 5:117449/1‑149 (MQ=255) aGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGCGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGAc < 6:365943/149‑1 (MQ=255) gtctGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGCTAGTCCGAAAAGCGCGGCAGATATTCCCGCGGAGTTGGCCCGCCGATCAACTCCAGCCGTAGAAATCGGGTTAAATTATGCTATGGCaaaaac > 4:80861/4‑273 (MQ=255) gTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCt > 2:510270/1‑111 (MQ=255) tctaaccatgtGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTCAATTATGCAGTGGCATAACAGCCTCCg > 1:293187/11‑279 (MQ=255) ggCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCAt < 5:843059/149‑1 (MQ=255) gCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCa < 7:1417709/103‑1 (MQ=255) gCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCa > 8:1417709/1‑103 (MQ=255) cAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATcc < 8:811675/149‑1 (MQ=255) aGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATccc > 6:1598656/1‑149 (MQ=255) aGCGACGGCGGCAGTCTGCCGAACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTcc < 2:636957/274‑1 (MQ=255) ccatgtGCGGCAGACTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCg > 7:928176/6‑149 (MQ=255) gACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGtt < 6:1154692/149‑1 (MQ=255) gACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGtt < 8:445888/149‑1 (MQ=255) gACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGtt < 5:1452183/149‑1 (MQ=255) gtGCGGCAGACTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTtg > 7:50453/2‑149 (MQ=255) cTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCa > 6:314283/1‑96 (MQ=255) tGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTtga < 7:1049919/149‑1 (MQ=255) cAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGaaa > 6:1317201/1‑149 (MQ=255) tCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAAtt > 5:874145/1‑149 (MQ=255) | GCGCACTCTCCACATAACCTAGCGCGCCAAGAGCATTAGAACCGCCAACCGGAATGACATACGGGCGAAAGCCTTGTGCTTCGACTCGCGTCGCCAGCTCTTCCAGTTGGGCATTGGGATCGGTCAGTGCGTCGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAATTTCCCGTCCTAGATAATCAGAAAAGCGCGGCAGATATTCGAGCGGCGTTGGCGCGCCGATAAACTCCAGCCGTGGAAAACGGGTTAAATTATGCAGTGGCATAACAGCCTCCGAT > NC_000913/1998961‑1999493 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |